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【Objective】The objective of this paper was to understand the content and enrichment characteristics of trace elements in soil and plant(root) of wild Berberis in Guizhou Province and to provide the theoretical reference for cultivation of wild Berberis. 【Method】The contents of trace elements in soil and plant(root) were determined, and the correlation of trace elements between soil and plant(root) and the enrichment ability of plant(root) to trace elements were discussed. 【Result】Total amount of soil trace elements of Berberis guizhouensis Ying, B. pruinosa var. barresiana Ahrendt, B. weiningensis Ying, B. tsienii Ying and B. deinacantha Schneid. was Fe > Mn > Zn > Cu > Mo; B. veitchii Schneid., B. wilsonae var. guhtzunica(Ahrendt) Ahrendt, B.pingbaensis M.T.An was Fe>Mn>Cu>Zn>Mo. The highest content of the soil total amount of Cu was B. veitchii, the total amount of Fe was B. tsienii, the total amount of Mn was B. weiningensis, the total amount of Mo was B. deinacantha, the total amount of Zn was B. weiningensis. The highest content of the soil available amount of Cu was B. guizhouensis, the available amount of Fe was B. veitchii, the available amount of Mn and Mo was B. weiningensis, the available amount of Zn was B.wilsonae var.guhtzunica.The highest content of Cu and Mn in the roots of was B.wilsonae var.guhtzunica., Fe was B. deinacantha, Mo was B. deinacantha, Zn was B. tsienii. 【Conclusion】The trace elements between soil and plant(root) were correlated to some extent. The enrichment coefficient of Cu of B. guizhouensis was the highest and was greater than 1, and the other trace elements enrichment coefficient was less than 1.The content of the available trace elements in the soil of non-area of Berberis in Guizhou Province was more obvious than that of the whole area. [ABSTRACT FROM AUTHOR]