1. A paper-based lateral flow immunochromatographic sensor for the detection of tricyclazole in rice.
- Author
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Liu, Yang, Guo, Lingling, Liu, Liqiang, Xu, Liguang, Kuang, Hua, Xu, Xinxin, and Xu, Chuanlai
- Subjects
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BROWN rice , *FLOW sensors , *RICE blast disease , *GOLD nanoparticles , *DETECTION limit - Abstract
Tricyclazole is commonly used to prevent rice blast to meet the carbohydrate intake needs of half of the global population, and a large number of toxicological reports indicate that monitoring of tricyclazole is necessary. Here, we analyzed the structure of tricyclazole and designed different hapten derivatization strategies to prepare a high-performance monoclonal antibody (half inhibition concentration of 1.61 ng/mL), and then a lateral flow immunochromatographic sensor based on gold nanoparticles for the detection of tricyclazole in rice, with a limit of detection of 6.74 μg/kg and 13.58 μg/kg in polished and brown rice, respectively. The recoveries in rice were in the range of 84.6–107.4%, no complex pretreatment was required for comparison with LC-MS/MS, and the comparative analysis demonstrated that our method had good accuracy and precision. Therefore, the developed lateral flow immunochromatographic analysis was a reliable and rapid means for the on-site analysis of tricyclazole in rice. • The anti-tricyclazole monoclonal antibody possessed a half-maximal inhibitory concentration of 1.61 ng/mL. • A paper-based lateral flow immunochromatographic sensor for the rapid and on-site analysis of tricyclazole was developed. • The sensor provided a limit of detection of 6.74 μg/kg and 13.58 μg/kg in polished and brown rice, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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