Search

Showing total 2 results
2 results

1. Molecular PET Imaging of Cyclophosphamide Induced Apoptosis with 18F-ML-8.

Author

Yao, Shaobo, Hu, Kongzhen, Tang, Ganghua, Gao, Siyuan, Tang, Caihua, Yao, Baoguo, Nie, Dahong, Sun, Ting, and Jiang, Shende

Subjects
*ADENOCARCINOMA, *CANCER treatment, *ANIMAL experimentation, *APOPTOSIS, *BIOLOGICAL models, *BIOPHYSICS, *DEOXY sugars, *HIGH performance liquid chromatography, *IMMUNOASSAY, *INJECTIONS, *INTRAVENOUS therapy, *RESEARCH methodology, *MICE, *PROBABILITY theory, *RADIOPHARMACEUTICALS, *RESEARCH funding, *STAINS & staining (Microscopy), *POSITRON emission tomography, *TUMORS, *XENOGRAFTS, *CYCLOPHOSPHAMIDE, *DATA analysis software, *DESCRIPTIVE statistics, *IN vitro studies, *ONE-way analysis of variance
Abstract

In this paper, a novel small-molecular apoptotic PET imaging probe, 18F-ML-8 with a malonate motif structure, is presented and discussed. After study, the small tracer that belongs to a member of ApoSense family is proved to be capable of imaging merely apoptotic regions in the CTX treated tumor-bearing mice. The experimental result is further confirmed by in vitro cell binding assays and TUNEL staining assay. As a result, 18F-ML-8 could be used for noninvasive visualization of apoptosis induced by antitumor chemotherapy. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

2. Myocardial lipidomics profiling delineate the toxicity of traditional Chinese medicine Aconiti Lateralis radix praeparata.

Author

Cai, Yamei, Gao, Yue, Tan, Guangguo, Wu, Si, Dong, Xin, Lou, Ziyang, Zhu, Zhenyu, and Chai, Yifeng

Subjects
*LIPID metabolism, *HEART analysis, *MEDICINAL plants, *ALTERNATIVE medicine, *ANIMAL experimentation, *ARRHYTHMIA, *BIOMARKERS, *BIOCHEMISTRY, *BIOPHYSICS, *COMPARATIVE studies, *DOSE-effect relationship in pharmacology, *DRUG toxicity, *ELECTROCARDIOGRAPHY, *HISTOLOGICAL techniques, *LIQUID chromatography, *MASS spectrometry, *PHENOMENOLOGY, *RESEARCH methodology, *LIPID peroxidation (Biology), *MICE, *MYOCARDIUM, *PHOSPHOLIPIDS, *RESEARCH funding, *PLANT roots, *T-test (Statistics), *PLANT extracts, *PILOT projects, *STATISTICAL significance, *DATA analysis software, *DESCRIPTIVE statistics
Abstract

Abstract: Ethnopharmacological relevance: The lateral root of Aconitum has been popularly used in traditional Chinese medicine (TMC) known as Fuzi which is beneficial for the treatment of various diseases, such as rheumatism, painful joints, syncope and bronchial asthma. However, it has a potential carditoxicity with a relatively narrow margin of safety. Aim of the study: This paper was designed to explore the mechanisms of Fuzi's toxicity and find out potential tissue-specific biomarkers of toxic effects. Material and methods: A myocardial lipidomics based on ultraperformance lipid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF MS) was developed to compare three cardiac lipid extraction methods and investigate the changes of lipids in mice heart of three different dosage groups. In addition, we concurrently inspected the biochemical parameters in plasma, observed the histology of the heart and recorded the electrocardiogram (ECG). Results: The cardiotoxicity of Fuzi was dose-dependent, and the high-dose group obviously manifested the heart damage in histology and a certain degree of arrhythmia. Significant changes of 14 lipid metabolites which primarily involved in phospholipid metabolism, sphingolipid metabolism, saturated fatty acid oxidation and unsaturated fatty acid peroxidation were identified and considered as the potential biomarkers of Fuzi toxicity. Conclusion: The lipidomics approach is helpful to search potential tissue-specific biomarkers and understand the underlying mechanisms of Fuzi toxicity on the heart. [Copyright &y& Elsevier]

Published
2013
Full Text
View/download PDF