1. Single-Nucleotide Polymorphisms and Markers of Oxidative Stress in Healthy Women.
- Author
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Minlikeeva, Albina N., Browne, Richard W., Ochs-Balcom, Heather M., Marian, Catalin, Shields, Peter G., Trevisan, Maurizio, Krishnan, Shiva, Modali, Ramakrishna, Seddon, Michael, Lehman, Teresa, and Freudenheim, Jo L.
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SINGLE nucleotide polymorphisms , *GENETIC markers , *OXIDATIVE stress , *WOMEN'S health , *MOLECULAR carcinogenesis , *THIOBARBITURIC acid test - Abstract
Purpose: There is accumulating evidence that oxidative stress is an important contributor to carcinogenesis. We hypothesized that genetic variation in genes involved in maintaining antioxidant/oxidant balance would be associated with overall oxidative stress. Methods: We examined associations between single nucleotide polymorphisms (SNPs) in MnSOD, GSTP1, GSTM1, GPX1, GPX3, and CAT genes and thiobarbituric acid-reactive substances (TBARS), a blood biomarker of oxidative damage, in healthy white women randomly selected from Western New York (n = 1402). We used general linear models to calculate age-adjusted geometric means of TBARS across the variants. We also examined the associations within strata of menopausal status. Results: For MnSOD, being heterozygous was associated with lower geometric means of TBARS (less oxidative stress), 1.28 mg/dL, compared to homozygous T-allele or homozygous C-allele,1.35 mg/dL, and 1.31 mg/dL correspondingly (p for trend = 0.01). This difference remained among postmenopausal women, 1.40 mg/dL for TT, 1.32 mg/dL for TC, and 1.34mg/dL for CC (p for trend 0.015); it was attenuated among premenopausal women. SNPs in the other genes examined (GSTP1, GSTM1, GPX1, GPX3, and CAT) were not associated with TBARS. Conclusions: Our findings suggest that genetic variation in MnSOD gene may be associated with oxidative status, particularly among postmenopausal women. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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