1. Ral GTPase is essential for actin dynamics and Golgi apparatus distribution in mouse oocyte maturation.
- Author
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Sun, Ming-Hong, Hu, Lin-Lin, Zhao, Chao-Ying, Lu, Xiang, Ren, Yan-Ping, Wang, Jun-Li, Cui, Xiang-Shun, and Sun, Shao-Chen
- Subjects
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GOLGI apparatus , *BIOLOGICAL transport , *OVUM , *GUANOSINE triphosphatase , *ACTIN , *SLEEP spindles , *BIRD migration - Abstract
Background: Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse oocyte maturation. Results: Our results showed that RalA/B expressed at all stages of oocyte maturation, and they were enriched at the spindle periphery area after meiosis resumption. The injection of RalA/B siRNAs into the oocytes significantly disturbed the polar body extrusion, indicating the essential roles of RalA/B for oocyte maturation. We observed that in the RalA/B knockdown oocytes the actin filament fluorescence intensity was significantly increased at the both cortex and cytoplasm, and the chromosomes were failed to locate near the cortex, indicating that RalA/B regulate actin dynamics for spindle migration in mouse oocytes. Moreover, we also found that the Golgi apparatus distribution at the spindle periphery was disturbed after RalA/B depletion. Conclusions: In summary, our results indicated that RalA/B affect actin dynamics for chromosome positioning and Golgi apparatus distribution in mouse oocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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