1. 趋化因子 CCL2 对人脐静脉内皮细胞 ICAM-1 表达的影响.
- Author
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刘丹, 田孝祥, 张效林, 高乃婧, and 闫承慧
- Abstract
Objective: To observe the effect of C-C motif ligand2 (CCL2) on the expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs cells were isolated and cultured in vitro. HUVECs were plated into 6-well plate, when cells were grown to 80-90 %, CCL2 overexpression plasmid [pcDNA3.1 (+)-CCL2] or CCL2 small interfering RNA (si-CCL2) was transfected into HUVECs. At different time points after transfection (12 h, 24 h and 48 h), HUVECs were collected for RNA and protein extraction. The mRNA expressions of CCL2 and ICAM-1 were examined using quantitative RT-PCR. The protein expressions of CCL2 and ICAM-1 were examined using western blotting. Results: (1) Compared with pcDNA3.1 (+) group, the expressions of CCL2 mRNA and protein were significantly higher in pcDNA3.1 (+)-CCL2 group; Compared with si-Control group, the expressions of CCL2 mRNA and protein were significantly decreased in si-CCL2 group. (2) Compared with pcDNA3.1 (+) group, pcDNA3.1 (+)-CCL2 significantly increased the mRNA and protein expressions of ICAM-1 in HUVECs, and si-CCL2 significantly inhibited the mRNA and protein expressions of ICAM-1 in HUVECs compared with si-Control group. Conclusions: CCL2 could affect ICAM-1 expression in HUVECs, which may provide a theoretical basis for clarifying the mechanisms of atherosclerosis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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