1. MicroRNA-361-5p Inhibits Tumorigenesis and the EMT of HCC by Targeting Twist1.
- Author
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Yin, Liang-Chun, Xiao, Gang, Zhou, Rui, Huang, Xiao-Ping, Li, Ning-Lei, Tan, Can-Liang, Xie, Feng-Jiao, Weng, Jun, and Liu, Li-Xin
- Subjects
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CARCINOGENESIS , *CELL proliferation , *CELL lines , *HEPATOCELLULAR carcinoma , *OXIDOREDUCTASES , *POLYMERASE chain reaction , *TRANSCRIPTION factors , *WESTERN immunoblotting , *BIOINFORMATICS , *DISEASE progression , *MICRORNA , *EPITHELIAL-mesenchymal transition - Abstract
MicroRNA-361-5p (miR-361-5p) is a tumor suppressor miRNA that is dysregulated in several types of human cancer. However, the functional significance of miR-361-5p in hepatocellular carcinoma (HCC) is unclear. This study explored the biological function of miR-361-5p in regulating the progression of HCC and the underlying molecular mechanism. RT-qPCR analysis showed that miR-361-5p was downregulated in HCC tissues and cell lines. Functional analysis revealed that miR-361-5p acted as a tumor suppressor, inhibiting cell proliferation, migration, and invasion in HCC cell lines. Bioinformatics analyses identified Twist1 as a direct target of miR-361-5p, which was validated by dual-luciferase reporter assays, RT-qPCR, and western blotting. Rescue experiments indicated that Twist1 may mediate the tumor-suppressive effect of miR-361-5p in HCC cells, and this was supported by the effect of miR-361-5p on inhibiting the epithelial-mesenchymal transition (EMT) by targeting Twist1. This study is the first to suggest that miR-361-5p inhibits tumorigenesis and EMT in HCC by targeting Twist1. These findings are valuable for the diagnosis and clinical management of HCC. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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