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Landfills are engineered, heterogeneously contaminated sites containing large reservoirs of paper waste. Cellulose degradation is an important process within landfill microbial ecology, and these anoxic, saturated environments are prime locations for discovery of cellulases that may offer improvements on industrial cellulose degradation efforts. We sampled leachate from three locations within a municipal landfill, a leachate collection cistern, and groundwater from an adjacent aquifer to identify cellulolytic populations and their associated cellulases. Metagenomic sequencing identified wide-spread and taxonomically diverse cellulolytic potential, with a notable scarcity of predicted exocellulases. 16S rRNA amplicon sequencing detected nine landfill microorganisms enriched in a customized leachate medium amended with microcrystalline cellulose or common paper stocks. Paper-enrichment cultures showed competition dynamics in response to the specific composition (lignin: hemi-cellulose: cellulose) of the different paper stocks. From leachate biomass, four novel cellulolytic bacteria were isolated, including two with the capacity for cellulolysis at industrially relevant temperatures. None of the isolates demonstrated exocellulase activity, consistent with the metagenome-based predictions. However, there was very little overlap between metagenome-derived predicted cellulolytic organisms, organisms enriched on paper sources, or the isolates, suggesting the landfill cellulolytic community is at low abundance but able to rapidly respond to introduced substrates. [ABSTRACT FROM AUTHOR]

To study the impact of faults on the enrichment and mobility of shale oil in the Gulong area, representative rock samples were selected in this paper. Based on geochemical data and chemical kinetics methods, coupled with shale oil enrichment and mobility analysis techniques, the shale oil generation quantity and in situ oil content were evaluated from the perspectives of shale oil generation and micro migration, and the mobility of shale oil was revealed. At the same time, the hydrocarbon expulsion efficiency (HEE) of shale was qualitatively and quantitatively characterized, combined with the development of faults. The research results indicate that the study area mainly develops organic-rich felsic (ORF)/organic-containing felsic (OCF) shale, their proportion in both wells exceeds 65%, and the resource amount is the largest in this type of lithofacies. The development of a fault controls the enrichment of shale oil, and the in situ oil content and oil saturation index (OSI) of the shale in well Y58, which is close to the fault, are significantly worse than those in well S2. Well Y58 has 9.52 mg/g and 424.83 mg/g TOC respectively, while well S2 has 11.34 mg/g and 488.73 mg/g TOC respectively. The fault enhanced the migration of shale oil, increasing the efficiency of oil expulsion. As a result, the components with weak polarity or small molecules, such as saturated hydrocarbons and low carbon number n-alkanes, are prone to migration, reducing the mobility of shale oil. [ABSTRACT FROM AUTHOR]

Aiming to address whether coal-bed methane and shale gas can form helium-rich gas reservoirs, this paper employs geochemical research methods to analyze the content of uranium (U) and thorium (Th) in coal and shale, as well as the helium content in coal-bed and shale gas reservoirs. An objective evaluation of the helium-generating potential and helium-bearing properties of coal and shale is provided. It is observed that although the content of U and Th in coal and shale is significantly higher than in other rocks, resulting in relatively more helium production from radioactive decay, the large amount of natural gas generated by coal and shale exerts a serious dilution effect on helium, making it difficult for coal beds and shale to enrich helium. The organic carbon content of coal is much higher than that of shale, leading to a greater generation of natural gas from coal beds compared to shale. Consequently, the helium content of coal-bed gas is much lower than that of shale gas. The helium rich shale gas and coal bed gas found in a few areas are attributed to the helium supply from other rocks in the gas reservoir, which is mostly distributed on or near the old granite masses, or in the tectonic active zones. In addition to capturing some of the helium produced by the coal beds and shales themselves, helium from other rocks, particularly from ancient basement rocks, is also captured, though this is not common. [ABSTRACT FROM AUTHOR]

Simple Summary: The B cell receptor (BCR) is a membrane-bound protein complex that is required for the normal development of B cells. BCR signalling is also involved in the pathogenesis of B cell cancers. While there is substantial literature on genomic analyses of the BCR, there are limited proteomic studies of receptor structure and its interactions with neighbouring proteins. This is partly due to the location of the BCR in the surface-membrane lipid environment that has limited the ability to enrich the complex for proteomic analysis. Here, we report an enrichment technique that can be used for mass spectrometry analyses of the BCR from live B cells. B cells are central to the adaptive immune response and provide long-lasting immunity after infection. B cell activation is mediated by the surface membrane-bound B cell receptor (BCR) following recognition of a specific antigen. The BCR has been challenging to analyse using mass spectrometry (MS) due to the difficulty of isolating and enriching this membrane-bound protein complex. There are approximately 120,000 BCRs on the B cell surface; however, depending on the B cell activation state, there may be hundreds-of-millions to billions of proteins in a B cell. Consequently, advanced proteomic techniques such as MS workflows that use purified proteins to yield structural and protein-interaction information have not been published for the BCR complex. This paper describes a method for enriching the BCR complex that is MS-compatible. The method involves a Protein G pull down on agarose beads using an intermediary antibody to each of the BCR complex subcomponents (CD79a, CD79b, and membrane immunoglobulin). The enrichment process is shown to pull down the entire BCR complex and has the advantage of being readily compatible with further proteomic study including MS analysis. Using intermediary antibodies has the potential to enrich all isotypes of the BCR, unlike previous methods described in the literature that use protein G-coated beads to directly pull down the membrane IgG (mIgG) but cannot be used for other mIg isotypes. [ABSTRACT FROM AUTHOR]

Simple Summary: Contemporary best practices for working with exhibit animals in zoological facilities incorporate behavioral research, theories on operant learning, animal welfare principles, and practical experience to provide animals with largely positive experiences when interacting with their caretakers and their environment. These best practices must be more fully implemented to increase ambassador animal welfare based on taxonomic and individual animal characteristics, experience with present and past caretakers, and ambassador program objectives. This implementation requires training staff to modify animal behavior using the most positive and least intrusive interactions possible. We rigorously consider how to apply best practices to meet ambassador program objectives and animal welfare needs by ensuring appropriate institutional support, the selection of the most suitable animals for the program's objectives, and the creation of an environment where animals recognize that they can choose whether to interact with humans and participate in programs, as well as exert control over their environment. Our synthesis provides a framework showing how staff can continually assess and address the behavioral response of ambassador animals to their environment—along with their interactions with humans during and outside of program usage—with the goal of meeting our most current and evolved criteria for ensuring high animal welfare. There is an ethical need to document and develop best practices for meeting ambassador animals' welfare needs within the context of meeting zoo and aquarium program objectives. This is because ambassador animals experience direct and frequent contact with humans. This paper rigorously synthesizes behavioral research and theory, contemporary practices, and personal experiences to offer key concepts that can be applied to meet ambassador animal welfare needs. These key concepts include addressing an animal's recognition of choice and control, the use of the most positive and least intrusive effective interventions when training animals to participate in programming, and an overall reduction in aversive strategy use. Our model for increasing ambassador animal welfare focuses on seven main areas of concern, including the following: choosing the most suitable animal for the program; choosing the human with the right skills and knowledge for the program; using the most positive, least intrusive, effective training methods; developing a strong trusting relationship between trainer and animal; developing a comprehensive enrichment program; the need for institutional support; and creating opportunities for animals to practice species-appropriate behaviors. Our model will provide guidelines for improved ambassador animal welfare that can be refined with future research. [ABSTRACT FROM AUTHOR]

Rare earth elements (REEs) have attracted much attention because of their unique physical and chemical properties. The root system is the plant organ most directly in contact with REEs, and it is critical to understand the mechanisms of interaction between the two. This paper investigates the effects of REEs on plant enrichment and fractionation, as well as on various developmental and toxicity indices of the root system. REEs are more likely to be deposited on the root surface under the influence of root secretion. The complexation between the two affects the uptake and fractionation of REEs and the altered pattern of root secretion. The toxicity mechanisms of REEs on plant root cells were lied in: (1) REEs generate reactive oxygen species after entering the plant, leading to oxidative stress and damage to plant cells; (2) REEs with higher charge-to-volume ratios compete for organic ligands with or displace Ca2+, further disrupting the normal function of plant root cells. It was shown that the sensitivity of inter-root microorganisms to REEs varied depending on the content and physicochemical properties of REEs. The paper also concluded with a meta-analysis of phytotoxicity induced by REEs, which showed that REEs affect plant physiological parameters. REEs, as a source of oxidative stress, triggered lipid peroxidation damage in plants and enhanced the activity of antioxidant enzymes, thus revealing the significant toxicity of REEs to plants. The phytotoxic effects of REEs increased with time and concentration. These results help to elucidate the ecotoxicology of rare earth-induced phytotoxicity. [Display omitted] • REEs mainly accumulate in roots with LREEs easily moving into above-ground plants. • Stimulated by REEs, the composition and exudation of root secretions can be altered. • REEs affect root cell function by displacing Ca2+ and inducing ROS. • Interactions between root secretions, microbes, and REEs influence plant growth. • Meta-Analysis reveals the diverse mechanisms of REEs-induced phytotoxicity. [ABSTRACT FROM AUTHOR]

Quaternary shale gas resources are abundant in the world, but Quaternary shale contains a lot of pore water, which affects the enrichment of shale gas. At present, the controlling effect of pore water on gas enrichment in Quaternary shale is not clear. Taking the Quaternary shale of Qaidam Basin, China as an example, this paper systematically studies the characteristics of pore water in Quaternary shale through X-ray diffraction rock analysis, nuclear magnetic resonance, methane isothermal adsorption and other experiments, and reveals the controlling effect of pore water on shale gas enrichment. The results show that clay shale and silty shale are mainly developed in Quaternary shale. The clay shale is more hydrophilic, and water mainly exists in micropores and mesopores. Silty shale is less hydrophilic, and water mainly exists in mesopores and macropores. Pore water controls the formation of shale gas by the content of potassium and sodium ions, controls the adsorption of shale gas by occupying the adsorption point on the pore surface, controls the flow of shale gas by occupying the pore space, and controls the occurrence of shale gas by forming water film. Therefore, pore water has multiple controlling effects on shale gas enrichment. This achievement is significant in enriching shale gas geological theory and guide shale gas exploration. [ABSTRACT FROM AUTHOR]

The paper presents laboratory and pilot flotation tests of the R-66 reagent on samples of mixed copper ore from the Konyrat deposit. The medium regulator reagent was supplied to the main flotation. Laboratory tests of the technology of flotation concentration on ore with a copper content of 0,31 % in a closed cycle in the optimal mode: R-66 - 100 g/t, sodium sulfide - 150 g/t, xanthate - 45 g/t, blowing agent - 60 g/t allows to extract copper into concentrate up to 85 %. At the same time, the copper content in the concentrate after two cleanings was reached at the level of 4,5 %, sulfur 42,3 %. At the same time, the R-66 reagent (100 g/t) makes it possible to completely eliminate the use of lime (1 000 g/t) in the ore dressing scheme. [ABSTRACT FROM AUTHOR]

Lead is one of the most toxic heavy metals released into the environment through industrial sources. Its direct determination is often a problem due to the presence of relatively complex matrices as well as low content. Thus, the additional separation and preconcentration steps are necessary in the analytical procedures. Carbon nanotubes (CNTs) continue to attract significant interest for these purposes as they exhibit a high specific surface area, exceptional porosities, and numerous adsorption sites. The modified CNTs with active groups, reagents, or materials have been widely explored using more mutual interactions that can significantly improve their sorption capacity and selectivity. This paper summarizes the recent developments from 2017 in the application of carbon nanotubes for the separation of Pb(II) and its enrichment/removal from the matrix components. Attention is given to oxidized CNTs, their modification with complexing compounds, functionalization with metal oxides and polymers, new nanocomposites, and carbon nanotube membranes. [ABSTRACT FROM AUTHOR]

In recent years, as the demand for precision nutrition is continuously increasing, scientific studies have shown that high-purity eicosapentaenoic acid ethyl ester (EPA-EE) functions more efficiently than mixed omega-3 polyunsaturated fatty acid preparations in diseases such as hyperlipidemia, heart disease, major depression, and heart disease; therefore, the market demand for EPA-EE is growing by the day. In this paper, we attempt to review EPA-EE from a whole-manufacturing-chain perspective. First, the extraction, refining, and ethanolysis processes (fish oil and ethanol undergo transesterification) of EPA-EE are described, emphasizing the potential of green substitute technologies. Then, the method of EPA enrichment is thoroughly detailed, the pros and cons of different methods are compared, and current developments in monomer production techniques are addressed. Finally, a summary of current advanced strategies for dealing with the low oxidative stability and low bioavailability of EPA-EE is presented. In conclusion, understanding the entire production process of EPA-EE will enable us to govern each step from a macro perspective and accomplish the best use of EPA-EE in a more cost-effective and environmentally friendly way. [ABSTRACT FROM AUTHOR]

A modern approach to animal nutrition involves the use of sustainable and environmentally safe solutions with maximum efficiency and the provision of all required nutrients. It is necessary to search for new methods of animal feed formulation and enrichment to meet these requirements. This paper presents an innovative spray biosorption method fortification of feeds for laying hens with valuable micronutrients (Cu, Mn, Zn, and Fe). The study aimed to evaluate the effectiveness of the method in vitro (extraction in water and ammonium citrate) and in vivo tests on laying hens. Experiments were conducted on a laboratory scale (up to 1 kg of feed was enriched), semi-technical scales (up to 1000 kg of feed was enriched), and the possibility of enriching feed in a spouted bed column was also investigated to test this method for an industrial scale. The influence of process conditions on biosorption efficiency (total concentration of micronutrients in solution 5000–15,000 g/L, the low rate of enrichment liquid 3.33·10–8 to 1.33·10–7 m3/s, material spraying time 68–720 s) was also investigated. A group of 108 Lohmann Brown laying hens was randomly divided into six experimental groups (including a control group). Each group of hens was fed a compound feed in which all or part of their micronutrient requirements were replaced with material enriched by the innovative method for 90 days. Egg characteristics, sensory evaluation, and micronutrient content were analysed, and in most cases, better results were obtained than with feeds enriched by standard methods. High-quality eggs were obtained, and egg production reached 95–96 %. The amount of micronutrients in the egg content was higher than the standard value for conventional farming for all research groups. A significant improvement in eggshell quality was also observed. The results are promising, and micronutrient-enriched eggs can be functional foods with higher micronutrient content. • An innovative spray sorption method for enriching feed materials was developed. • The feed mixture was enriched with micronutrients Cu, Mn, Zn, and Fe. • Research was conducted on laboratory and semi-technical scales. • The obtained high-quality feed material was tested on laying hens. [ABSTRACT FROM AUTHOR]

In this paper, the mineralogical composition, concentrations, distribution, and modes of occurrence of the trace elements in coal from the Anjialing coal seam 9 in the Pingshuo mining district, Ningwu coalfield, were studied using optical microscopy, X-ray powder diffraction (XRD), inductively coupled plasma optical emission spectrometry (ICP-OES), inductively coupled plasma mass spectrometry (ICP-MS), and sequential chemical extraction procedures (SCEPs). The identified minerals included mainly kaolinite, boehmite, pyrite, calcite, quartz, and muscovite. Compared to other hard coal from around the world, the coal from seam 9 was enriched with lithium (Li); slightly enriched with gallium (Ga), hafnium (Hf), zirconium (Zr), and mercury (Hg); typically enriched with lead (Pb), and depleted in arsenic (As). The results of the SCEPs analysis showed that Li, Ga, Zr, and Hf were mainly associated with clay minerals. Arsenic mainly occurred in its silicate and sulfide forms in pyrite and Pb was mainly associated with aluminosilicate, sulfide, and carbonate minerals. [ABSTRACT FROM AUTHOR]

Interactions of microplastics (MPs) biofilm with antibiotic resistance genes (ARGs) and antibiotics in aquatic environments have made microplastic biofilm an issue of keen scholarly interest. The process of biofilm formation and the degree of ARGs enrichment in the presence of antibiotic-selective pressure and the impact on the microbial community need to be further investigated. In this paper, the selective pressure of ciprofloxacin (CIP) and illumination conditions were investigated to affect the physicochemical properties, biomass, and extracellular polymer secretion of polyvinyl chloride (PVC) microplastic biofilm. In addition, relative copy numbers of nine ARGs were analyzed by real-time quantitative polymerase chain reaction (qPCR). In the presence of CIP, microorganisms in the water and microplastic biofilm were more inclined to carry associated ARGs (2–3 times higher), which had a contributing effect on ARGs enrichment. The process of pre-microplastic biofilm formation might have an inhibitory effect on ARGs (total relative abundance up to 0.151) transfer and proliferation compared to the surrounding water (total relative abundance up to 0.488). However, in the presence of CIP stress, microplastic biofilm maintained the abundance of ARGs (from 0.151 to 0.149) better compared to the surrounding water (from 0.488 to 0.386). Therefore, microplastic biofilm act as abundance buffer island of ARGs stabilizing the concentration of ARGs. In addition, high-throughput analyses showed the presence of antibiotic-resistant (Pseudomonas) and pathogenic (Vibrio) microorganisms in biofilm under different conditions. The above research deepens our understanding of ARGs enrichment in biofilm and provides important insights into the ecological risks of interactions between ARGs, antibiotics, and microplastic biofilm. [Display omitted] • Characteristics of PVC biofilm formation by different conditions were summarized. • Selective pressure of CIP had enrichment effect on ARGs in water and biofilm. • PVC biofilm created conditions for selective pressure to generate ARGs. • Biofilm maintained the abundance of ARGs better than the surrounding water. • Characterization of bacterial community composition in PVC biofilm was studied. [ABSTRACT FROM AUTHOR]

Terrigenous nutrients discharged into seawater played an important role in controlling the marine primary productivity. Study on the horizontal and vertical distribution characteristics of nutrients was essential in eutrophication and sea-air exchange study. In this paper, the nutrient concentrations and their distribution in the surface microlayer and three sub-surface layers of the Yellow River estuary were measured. The horizontal and vertical distribution characteristic of nutrients was analyzed, so did the potential eutrophication assessment and the nutrient enrichment of the surface microlayer. The results showed that nutrient concentrations decreased from the estuary to the shore horizontally. NH4+-N and NO2--N increased vertically. NO3--N and PO4³-P decreased vertically. Eutrophication occurred in the micro-layer and the sub-surfaces. The Yellow River estuary suffered an eutrophication outbreak risk. The enrichment of NH4+-N, NO3--N and NO2--N was significant in April 2018, nor did PO43-- P. [ABSTRACT FROM AUTHOR]

In Pagin 2014 I provided a new account of pragmatic enrichment. Building on the theory of coherence relations defended by Andrew Kehler, I proposed a four step scale of coherence strength. According to the account, free enrichment takes place, subject to constraints, when it raises the degree of coherence. It turned out that there is an intriguing interaction between coherence raising and determiner semantics: certain determiners license coherence raising while others tend to block them. In this paper I investigate the phenomenon. I try to identify the determiner properties that license coherence raising, and provide an explanation of why they do. 1. A problem for an earlier theory by the author of pragmatic enrichments is noted and discussed. 2. It is observed that enrichments fail in certain quantifier contexts. 3. An explanation of this phenomenon is proposed. [ABSTRACT FROM AUTHOR]

In this paper, amphiphilic hydroxyapatite (HAP) nanoparticles were modified by dibutyl phosphate (DBP) via covalent bonding. The modified HAP particles (m-HAP) were employed as building blocks to construct oil-in-water (O/W) Pickering emulsion, that displayed an excellent performance on the enrichment of organic pollutants dissolved in wastewater by extracting the organic molecules into the oil phase. Environment-friendly organic solvent hexanol was selected as oil phase and three types of monochlorophenol (2-chlorophenol, 3-chlorophenol, 4-chlorophenol) were chosen as model pollutants in simulated wastewater. Two types of natural water were also tested as a proof of principle. The enrichment percentage of chlorophenols was up to 98% in 140 s, following first order kinetics. Thermodynamic study suggested that the enrichment process is spontaneous and exothermic. The external environment of the protocells, such as pH, ionic strength and the natural organic matter have been investigated. This study provides a novel, convenient and environment-friendly approach for enrichment and removal of trace organic pollutants in wastewater. Image 1 • A novel approach for enrichment of trace organic pollutants by protocells prepared from modified HAP nanomaterials. • Extremely speed and huge capacity of enrichment was achieved by self-assembled protocells. • Super anti-interference ability on the enrichment of chlorophenols. [ABSTRACT FROM AUTHOR]

Uranium metal and its compounds have been of great interest to physicists and chemists due to its use for both civil and military applications, e.g. production of electricity, use in the medical field and for making nuclear weapons. This review paper describes the occurrence, chemistry and metallurgy of the element 'uranium', its conversion to stable compounds such as yellow cake, uranium tetrafluoride and uranium hexafluoride and the enrichment technologies and uses for both civil and military purposes. The paper is meant for ready reference for students and teachers in connection with the recent spate of interest shown in nuclear power generation in Pakistan and abroad. [ABSTRACT FROM AUTHOR]

Early life events can exert a powerful influence on both the pattern of brain architecture and behavioral development. The paper examines the nature of nervous system plasticity, the nature of functional connectivities in the nervous system, and the application of connectography to better understand the concept of a functional neurology that can shed light on approaches to instruction in preschool and primary education. The paper also examines the genetic underpinnings of brain development such as synaptogenesis, plasticity, and critical periods as they relate to numerosity, language and perceptual development. Discussed is how the child's environment in school and home interact with and modify the structures and functions of the developing brain. The role of experience for the child is to both maintain and expand the child's early wiring diagram necessary for effective cognitive as well as neurological development beyond early childhood. [ABSTRACT FROM AUTHOR]

Microplastics (MPs) and Nanoplastics (NPs) accumulated in the environment have been identified as a major global issue due to their potential harm to wildlife. Current research in the detection of MPs is well established. However, the detection of NPs remains challenging. The aim of this paper is to investigate the detection of polystyrene (PS) NPs on a super-hydrophobic substrate using surface-enhanced Raman spectroscopy (SERS) technology after high-speed centrifugation of PS NPs and AgNPs. The hydrophobic substrate reduces the contact area of droplet, concentrating PS NPs and AgNPs on a small spot, which eliminates the random distribution of nano particles. The condensed PS NPs and AgNPs improve the SERS intensity, reproductivity and detection sensitivity. The results show that SERS measurement on a hydrophobic substrate could significantly improve the detection sensitivity of PS NPs, with the detection limits of PS NPs as low as 0.5 mg/L (500 nm PS NPs) and 1 mg/L (100 nm PS NPs). The study provides an effective and rapid method for the detection of NPs at trace concentration, demonstrating more possibility for the future detection of trace NPs in the aquatic environment. [Display omitted] • High speed centrifugation could enrich PS nanoparticles (NPs) in aqueous samples. • Nano particles can be condensed in a small spot on the hydrophobic substrate. • SERS intensity of PS NPs was improved using the super hydrophobic substrate. • The detection limits of PS NPs could reach as low as 1 mg/L. [ABSTRACT FROM AUTHOR]

The life cycle carbon emission factor (measured by t-CO 2 /GW h) of nuclear power is much lower than those of fossil fueled power generation technologies. However, the fact of nuclear energy being a low carbon power source comes with many assumptions. These assumptions range from system and process definitions, to input–output definitions, to system boundary and cut-off criteria selections, and life cycle inventory dataset. However, there is a somewhat neglected but critical aspect – the design aspect. This refers to the impacts on the life cycle carbon emissions from the change in design parameters related to nuclear power. The design parameters identified in this paper include: (1) the uranium ore grade, (2) the critical process technologies, represented by the average initial enrichment concentration of 235 U in the reactor fuel, and (3) the size of the nuclear power reactor (measured by the generating capacity). If not properly tested, assumptions in the design aspect can lead to an erroneous estimation on the life cycle carbon emission factor of nuclear power. In this paper, a methodology is developed using the Process Chain Analysis (PCA) approach to quantify the impacts of the changes in the selected design parameters on the life cycle carbon emission factor of nuclear power. The concept of doing so broadens the scope of PCAs on energy systems from “one-off” calculation to analysis towards favorable/preferred designs. The findings from the analyses can serve as addition to the life cycle inventory database for nuclear power as well as provide indications for the sustainability of nuclear energy systems. [ABSTRACT FROM AUTHOR]

A large number of materials exist which have been labeled as low activation structural materials (LAM). Most often, these materials have been designed in order to substitute-out or completely remove elements that become activated and contribute significantly to shut-down activity after being irradiated by neutrons in a reactor environment. To date, one of the fundamental principles from which LAMs have been developed is that natural elemental compositions are the building blocks of LAMs. Thus, elements such as Co, Al, Ni, Mo, Nb, N and Cu that produce long-lived decay products are significantly reduced or removed from the LAM composition. These elements have an important part to play in the composition of steels and the removal/substitution can have a negative impact on materials properties such as yield stress and fracture toughness. This paper looks in more detail at whether using isotopic selection of the more mechanically desirable, but prohibited due to activation, elements can improve matters. In particular, this paper focuses on the activation of Eurofer. Carefully chosen isotopically enriched elements, which are normally considered to be on the prohibited element list, are added to EUROFER steel as potential alloying elements. The EUROFER activation results show that some prohibited elements can be used as alloying elements in LAM steels, providing the selected isotopes do not have a significant impact on waste disposal rating or shut-down dose. The economic implications of isotopically enriching elements and the potential implications for decommissioning are considered. It is shown that the addition of molybdenum and nickel in small concentrations (<2% mass) could have the potential to increase availability to such an extent that the capital investment associated with isotope enrichment is negated and profits from electricity sale increased. Another important issue for materials exposed to neutron irradiation is the production of gases, in particular helium, as a result of nuclear interactions. Helium accumulation in materials can cause embrittlement and so mitigating the rate of production is an important consideration when selecting materials. The second part of this paper considers whether helium production can be reduced in CuCrZr by isotopic tailoring. CuCrZr is a candidate bonding material for tungsten at first wall locations, however it suffers from degradation due to helium production. Inventory calculations show that isotopically enriching the copper, in CuCrZr, can reduce helium production by approximately 50%. However, cost–benefit analysis suggests that the cost of enriching copper is not cost-effective due to the high price of electromagnetic enrichment that is required for copper. [ABSTRACT FROM AUTHOR]

Abstract: This paper describes the quantification of extracellular carbonic anhydrase (eCA) concentrations in the sea surface microlayer (SML), the boundary layer between the ocean and the atmosphere of the Indo‐West Pacific. We demonstrated that the SML is enriched with eCA by 1.5 ± 0.7 compared to the mixed underlying water. Enrichment remains up to a wind speed of 7 m s−1 (i.e., under typical oceanic conditions). As eCA catalyzes the interconversion of HCO3− and CO2, it has been hypothesized that its enrichment in the SML enhances the air‐sea CO2 exchange. We detected concentrations in the range of 0.12 to 0.76 nM, which can enhance the exchange by up to 15% based on the model approach described in the literature. [ABSTRACT FROM AUTHOR]

In this paper, the recent development of extended consecutive-interpolation 4-node quadrilateral element (XCQ4), which goes beyond certain limitations of conventional methods, is further employed to study dynamic and static thermoelastic fracture problems. In particular, static stress intensity factors (SIFs) and transient dynamic responses of two-dimensional (2-D) stationary cracks in elastic solids under thermal and thermal-mechanical loadings are investigated. In addition, simulation of quasi-static crack propagation under thermal-mechanical loading condition is also performed. To precisely capture the singularity and discontinuity of temperature, heat flux, and displacements due to the presence of crack, the unknown field variables of mechanical displacements and temperature are approximated in terms of the XCQ4 framework by different enrichment strategies, which include either the use of the conventional branch functions or the new ramp function associated with Heaviside function. Only adiabatic condition is considered on the crack surfaces. For quasi-static crack growth analysis, the maximum hoop stress criterion, which is also valid for thermo-elastic fracture mechanics, is adopted. For transient crack analyses, the inertial effect is taken into account for the interaction integral in the consideration of DSIFs. Through eight numerical examples for thermoelastic fracture problems, the validity of the XCQ4 is confirmed by a comparison of the obtained results to those derived from different approaches in previous studies. In addition, the accuracy, performance, and superior advantages of the XCQ4 over standard extended finite element approach are also demonstrated. [ABSTRACT FROM AUTHOR]

Purpose The conservation of resources (COR) theory provides a theoretical foundation for work-family research. The purpose of this paper is to investigate thoroughly the associations between threat of or actual loss of resources as well as gain of resources and work-family interaction, employing COR assumptions and measures.Design/methodology/approach A sample of 216 working mothers filled out a questionnaire that included conservation of resources evaluation and scales measuring work-family conflict (WFC) and enrichment. Analyses of variance were performed to test the hypothesized associations.Findings WFC and family-work conflict (FWC) were positively correlated with the threat of and actual loss of resources; family-work enrichment (FWE) was positively correlated with the gain of resources. Participants who reported higher threat of loss of resources compared to gain of resources reported high levels of WFC and FWC; those who reported higher loss of resources compared to gain of resources reported high levels of FWC. In addition, participants who reported gains that outweighed losses (whether actual loss or simply threat of loss) reported higher levels of FWE.Originality/value The findings support using the COR theory as a theoretical basis for work-family research and emphasize the detrimental role of threat of loss of resources. Theoretical and practical implications are discussed. [ABSTRACT FROM AUTHOR]

Simple Summary: Farm animal welfare is an important issue for both farmers and the general public. In order to monitor animal welfare on a large population of finishing pig farms in the UK, the "Real Welfare" project developed a methodology for on-farm assessment based on regular measurement of some key animal-based measures, so-called welfare outcomes. This paper presents estimates of the percentage of these different welfare outcomes over the years since the inception of the scheme. Between 2016 and 2019, the mean percentage of pigs in the mainstream herd showing four mandatory welfare outcomes (pigs requiring hospitalization, lame pigs, pigs with severe tail lesions, and pigs with severe body marks) was very low and a decreasing trend was observed for all outcomes except severe tail lesions. These data give good representation of the overall situation in the UK finishing pig population. Farm animal welfare is an increasingly important issue, leading to the need for an efficient methodology to deliver accurate benchmarking. The "Real Welfare" project developed a methodology based on regular recording of a limited number of animal-based measures, so-called welfare outcomes, which allows faster and easier on-farm assessment of finishing pig welfare. The objective of this paper is to estimate, with sufficient robustness and confidence, the prevalence of different mandatory and optional welfare outcomes in the mainstream herd of the finishing farms in the UK based on the "Real Welfare" scheme data and to assess the changes in prevalence over time, inspection visits and seasons. The mean overall prevalence of the four mandatory welfare outcomes (pigs requiring hospitalization, lame pigs, pigs with severe body marks, and pigs with severe tail lesions) was very low (≤0.2%) and a significant decreasing trend was observed for the first three of these mandatory welfare outcomes since the inception of the scheme. This result might reflect either a reduction in factors giving rise to welfare problems in the mainstream herd or increasing awareness about management of compromised pigs. Additional data are required to clarify these possibilities, but both represent improved pig welfare. [ABSTRACT FROM AUTHOR]

The Pennsylvanian coals in the UK were deposited in a low-lying delta plain which was periodically inundated by the sea. This resulted in high S contents, mainly due to early diagenetic pyrite. Based on a statistical analysis of the geochemical data, pyrite is the major host for As, Se, Hg, Tl and Pb and an important host for Mo, Cd, Ni, Sb and Cu. All of these elements are enriched in the coals compared with non-marine mudrocks in the sequence, as is Ge and Be, although theirs is an organic association. The pattern of enrichment in the coals is similar to that in marine shales and coals overlain by marine shales, demonstrating the seawater influence. Goldschmidt was a pioneer in the geochemical study of coals and this paper will demonstrate that many of his ideas have stood the test of time. [ABSTRACT FROM AUTHOR]

Organohalide-respiring bacteria can be difficult to enrich and isolate, which can limit research on these important organisms. The goal of this research was to develop a method to rapidly (minutes to days) enrich these organisms from a mixed community. The method presented is based on the hypothesis that organohalide-respiring bacteria would be more hydrophobic than other bacteria as they dehalogenate hydrophobic compounds. The method developed tests this hypothesis by separating a portion of putative organohalide-respiring bacteria, those phylogenetically related to Dehalococcoides mccartyi, at the interface between a hydrophobic organic solvent and an aqueous medium. This novel partial separation technique was tested with a polychlorinated biphenyl-enriched sediment-free culture, a tetrachloroethene-enriched digester sludge culture, and uncontaminated lake sediment. Significantly higher fractions, up to 20.4 times higher, of putative organohalide-respiring bacteria were enriched at the interface between the medium and either hexadecane or trichloroethene. The selective partial separation of these putative organohalide-respiring bacteria occurred after 20 min, strongly suggesting that the separation was a result of physical-chemical interactions between the cell surface and hydrophobic solvent. Dechlorination activity postseparation was verified by the production of cis-dichloroethene when amended with tetrachloroethene. A longer incubation time of 6 days prior to separation with trichloroethene increased the total number of putative organohalide-respiring bacteria. This method provides a way to quickly separate some of the putative organohalide-respiring bacteria from other bacteria, thereby improving our ability to study multiple and different bacteria of potential interest and improving knowledge of these bacteria. IMPORTANCE: Organohalide-respiring bacteria, bacteria capable of respiring chlorinated contaminants, can be difficult to enrich, which can limit their predictable use for the bioremediation of contaminated sites. This paper describes a method to quickly separate Dehalococcoides-like bacteria, a group of organisms containing organohalide-respiring bacteria, from other bacteria in a mixed community. From this work, Dehalococcoides-like bacteria appear to have a hydrophobic cell surface, facilitating a rapid (20 min) partial separation from a mixed culture at the surface of a hydrophobic liquid. This method was verified in a polychlorinated biphenylenriched sediment-free culture, an anaerobic digester sludge, and uncontaminated sediment. The method described can drastically reduce the amount of time required to partially separate Dehalococcoides-like bacteria from a complex mixed culture, improving researchers' ability to study these important bacteria. [ABSTRACT FROM AUTHOR]

Introduction: Bread is the staple food of most Muslims and can be considered to be a component with a remarkable effect on satiety and appetite during Ramadan fasting. This study aimed to present the recent advances in investigating the effect of different types of bread and enrichments on satiety and appetite. Methods: In this paper, articles focusing on the effect of various bread types (including enriched bread) on satiety and appetite, particularly during fasting were reviewed. Articles were found in databases such as ISI, PubMed and Google Scholar. Results: Different bread types with lower glycemic index are recommended for Ramadan fasting, especially for the Sahur meal, due to better satiety and sglycemic control. In addition, fermentable dietary fibers, such as arabinoxylans, ß-glucan, fructans, and resistant starch, can influence appetite through fermentation in the colon by saccharolytic bacteria and gastrointestinal tract releasing hormones changes. Consumption of wholemeal bread results in the moderation of satiety and starvation. Barely, oat and rye breads demonstrate the better improvement of satiety compared to white wheat bread due to their higher fiber content, probiotic ingredients and steadier glycemia. On the other hand, use of protein-rich breads can result in delayed gastric emptying, steadier insulin levels and higher satiety. Beta-glucan enrichment shows similar significant results in terms of reducing hunger and increasing satiety by influencing the appetite and satiety and regulating hormones such as insulin, ghrelin and PYY. Fiber enrichment and probiotics (Fructo-oligosaccharides/Inulin) could also be considered in this regard. Conclusion: During Ramadan fasting, barley bread, oat bread and wholegrain wheat bread could be suggested as the preferred bread types to be incorporated into the main meals to improve satiety and decrease hunger feeling. [ABSTRACT FROM AUTHOR]

A simple, affordable point of care test (POCT) is necessary for on-site detection of coronavirus disease 2019 (COVID-19). The lateral flow assay (LFA) has great potential for use in POCT mainly because of factors such as low time consumption, low cost, and ease of use. However, it lacks sensitivity and limits of detection (LOD), which are essential for early diagnostics. In this study, we proposed a non-powered preconcentrator (NPP) based on nanoelectrokinetics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Antigen (Ag) lateral flow assay. The non-powered preconcentrator is composed of glass fiber-based composite paper and ion permselective material, and it can be simply operated by force balancing gravitational, capillary, and depletion-induced forces. The proposed approach helps enrich the SARS-CoV-2 viral nucleocapsid (N) proteins based on a 10-min operation, and it improved the LOD by up to 10-fold. The corresponding virus enrichment, which was evaluated using the reverse-transcriptase polymerase chain reaction (RT-PCR), revealed an improvement in ΔCt values > 3. We successfully demonstrated the enhancement of the NPP-assisted LFA, we extended to applying it to clinical samples. Further, we demonstrated an affordable, easy-to-implement form of LFA by simply designing NPP directly on the LFA buffer tube. [ABSTRACT FROM AUTHOR]

This paper is concerned with the prediction of heat transfer in composite materials with uncertain inclusion geometry. To numerically solve the governing equation, which is defined on a random domain, an approach based on the combination of the Extended finite element method (X-FEM) and the spectral stochastic finite element method is studied. Two challenges of the extended stochastic finite element method (X-SFEM) are choosing an enrichment function and numerical integration over the probability domain. An enrichment function, which is based on knowledge of the interface location, captures the C-continuous solution in the spatial and probability domains without a conforming mesh. Standard enrichment functions and enrichment functions tailored to X-SFEM are analyzed and compared, and the basic elements of a successful enrichment function are identified. We introduce a partition approach for accurate integration over the probability domain. The X-FEM solution is studied as a function of the parameters describing the inclusion geometry and the different enrichment functions. The efficiency and accuracy of a spectral polynomial chaos expansion and a finite element approximation in the probability domain are compared. Numerical examples of a two-dimensional heat conduction problem with a random inclusion show the spectral PC approximation with a suitable choice of enrichment function is as accurate and more efficient than the finite element approach. Though focused on heat transfer in composite materials, the techniques and observations in this paper are also applicable to other types of problems with uncertain geometry. [ABSTRACT FROM AUTHOR]

This paper statistically quantifies the lifecycle greenhouse gas (GHG) emissions from six distinct reactor-based (boiling water reactor (BWR), pressurized water reactor (PWR), light water reactor (LWR), heavy-water-moderated reactor (HWR), gas-cooled reactor (GCR), fast breeder reactor (FBR)) nuclear power generation systems by following a two-step approach that included (a) performing a review of the lifecycle assessment (LCA) studies on the reactor-based nuclear power generation systems; and (b) statistically evaluating the lifecycle GHG emissions (expressed in grams of carbon dioxide equivalent per kilowatt hour, gCO2e/kWh) for each of the reactor-based nuclear power generation systems to assess the role of different types of nuclear reactors in the reduction of the lifecycle GHG emissions. Additionally, this study quantified the impacts of fuel enrichment methods (centrifuge, gaseous diffusion) on GHG emissions. The mean lifecycle GHG emissions resulting from the use of BWR (sample size, N = 15), PWR (N = 21), LWR (N = 7), HWR (N = 3), GCR (N = 1), and FBR (N = 2) in nuclear power generation systems are 14.52 gCO2e/kWh, 11.87 gCO2e/kWh, 20.5 gCO2e/kWh, 28.2 gCO2e/kWh, 8.35 gCO2e/kWh, and 6.26 gCO2e/kWh, respectively. The FBR nuclear power generation systems produced the minimum lifecycle GHGs. The centrifuge enrichment method produced lower GHG emissions than the gaseous diffusion enrichment method. [ABSTRACT FROM AUTHOR]

Phytomining of noble metals (NMs) offers a promising possibility of metal extraction at sites where traditional mining activities or recovering NMs from low-grade minerals are not competitive. In addition to conventional mining, producing NMs from secondary resources strengthening the circular economy has been paid worldwide attention. The review presented in this paper links three scientific areas as the essential elements to form the phytomining chain of NMs. The accumulation of NMs in plants is the first step, referred as the phytoextraction process. This is followed by heightening the concentration of NMs via the enrichment stage. Eventually, although less well understood, extraction methods of NMs from biomass solid remains as well as from diverse secondary sources particularly incineration ashes are discussed that assist to visualize the potential pathways in phytomining. •Processes and opportunities of noble metals (NMs) phytomining were deeply researched. The review presented in this paper link scientific areas as essential elements to form the phytomining chain of NMs. • Prize and market analysis of NMs to determine the demand side of phytomining. •Summary of phytoextraction and phytomining research and results. •Review and comparison of enrichment and extraction methods of NMs. •Focusing on the review of NMs extraction from solid biomass remains. •Analysis of profitable grades and concentration of NMs before extraction methods. [ABSTRACT FROM AUTHOR]

Relevance theorists and others have argued that what a speaker ‘says’ is determined by contextual factors to a greater extent than allowed for by Grice. This dispute has consequences for what Grice called generalized conversational implicatures. Contextualists have argued that the propositions that Grice thinks of as implicated in a generalized way should be treated as part of what is said. Griceans, on the other hand, by insisting that these are conversationally implicated, are committed to postulating more minimal propositions for the role of what is said. This paper examines whether such minimal propositions play a role in utterance understanding. Three pragmatic processing models, which embody some of the assumptions of the rival philosophical views, are postulated. The results of a series of experiments designed to test the predictions of these processing models are reported. Our results lend some initial support to a model in which minimal and contextually enriched interpretations are constructed in parallel, with a bias towards the enriched interpretations. However, the final section of the paper suggests a number of ways in which the issue of the role of minimal propositions still remains open for empirical exploration. [Copyright &y& Elsevier]

Measurement of natural stable isotope signatures of insects has proven to be a useful method in determining their natal origin, feeding strategies and mating behavior, and has spawned a new discipline of insect isotope forensics. However the complexity of measuring isotope signatures using elemental analysis-isotope ratio mass-spectrometry (EA-IRMS) has encountered significant technical barriers, which together with costs, have hindered the advancement and wide spread adoption of this potentially useful approach. In the last decade simpler technologies to measure stable isotope ratios in samples based on laser spectroscopy have been developed. We foresee that these instruments will fuel the advancement and adoption of insect isotope forensics into pest management programs because they are easy to use, more affordable, and complement existing marking tools. However prior to the adoption of these new technologies it is necessary to test their fitness for purpose and explore their limitations. In this paper we set out to test the suitability of using combustion module-cavity ring-down spectroscopy (CM-CRDS) for measuring isotope ratios in an entomological context, covering marking of insects, mating behavior and dietary assessment. We carried out a series of experiments to compare the isotope measurements made with the CM-CRDS with the traditional method of EA-IRMS. Initially we compared measurements of carbon isotope values of a variety of common diet components used in insect rearing, which covered a wide range of carbon isotope values. There was good agreement in the isotope values obtained, using the CM-CRDS and the EA-IRMS with similar accuracy and precision. Secondly we compared the 2 methods to measure carbon isotope values of the common cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) samples, and again there was good agreement in the values obtained. Thirdly we tested whether the CM-CRDS could be used to determine the paternity of spermataphores, based on the differential isotope signatures of the paternal and maternal moths, as a result of differences in their rearing diets. The CM-CRDS proved to be fit for the purpose of measuring isotope values in the spermataphores of common cutworm and suggested that the CM-CRDS technology would be suitable for these isotope based mating studies in moths if the spermatophores were of sufficient mass. Finally we explored a number of the issues surrounding CM-CRDS and isotope technologies in general, e.g., logistical considerations, economics of operation, sample size, etc. We conclude that CM-CRDS is a suitable instrument for measuring stable isotope carbon signatures in moths, and most probably other sufficiently large insects and that CM-CRDS could be easily used in both operational and research contexts. [ABSTRACT FROM AUTHOR]

In this paper, we report that fast evaporation of volatile solvent from cellulose acetate (CA) solution layer containing a proper nonvolatile alcohol can lead to the formation of relatively uniform nanoporous CA membrane. We propose that in the phase-separation process, the molecules of the nonvolatile alcohol aggregate to constitute the polymer-lean nuclei (PLN). These PLN exist stably (but not evaporate), grow continuously, and develop finally into the pores on the membrane. Relevant evidences are provided to support the proposed mechanism. This work implies a possible approach to generate uniform nanopores on polymer membrane. [ABSTRACT FROM AUTHOR]

Microsatellites or simple sequence repeats (SSRs) consist of tandem repeats of di- to pentanucleotide motifs with conserved flanking sequences. These conserved sequences have been used for designing suitable primers for amplification of the SSR loci using PCR and reflects polymorphism in the form of differences in the length of amplified product. Microsatellite markers were developed in Luffa cylindrica using the biotin-streptavidin enrichment approach. Plasmid DNA was isolated from 100 white colonies and out of which 50 colonies were selected for sequencing. The sequenced fragments were checked for vector contamination using VecScreen software and most of them were found contaminated. Only 11 were without contamination but no microsatellite repeats were found within these sequences, when analyzed with WebSat software. This paper reports the first attempt of isolation of microsatellite markers in Luffa through enrichment method. [ABSTRACT FROM AUTHOR]

Production of chicken meat is an important branch of food industry. Generally, poultry meat farmers aim to produce birds with superior body weight and feed conversion ratio. However, in line with current developments, there are other parameters that must to be taken into account such as lower body fat deposition and improvement in the nutrient composition of products corresponding to consumer requirements. Nowadays, extra focus has been given to designing and enrichment foods as functional foods, with components that have beneficial effects on human wellbeing. In definition, functional foods contain particular nutrients and / or non-nutrients that have an effect on human health, beyond what is usually known as nutritional effects. There are several compounds in foods that improve consumer's health status. Among them the polyunsaturated fatty acids (PUFAs, n-6 and n-3 series) and conjugated linoleic acid (CLA) are the most functional and bioactives components of lipids. Chicken meat has been a common objective of nutritional modification because the absorbed dietary fatty acids from the small intestine directly enter into the chicken tissue lipids. This paper tries to review the recent findings in the area of chicken meat enrichment using dietary fats. [ABSTRACT FROM AUTHOR]

The increasing demand for rare earth elements (REEs) for modern industry has led to a surge in mining activities and consequently has released these metals into the environment. Intensifying REEs in a habitat has impacts on its ecosystem, but on the other side, it also provides the opportunity to recover REEs from low-grade minerals. Phytomining has emerged as an ecologically sound technique to extract these valuable elements from contaminated soils where traditional mining is not competitive. This paper presents and reviews the concept of REE phytomining from three scientific areas. The accumulation of rare earth metals in plants is the first stage, referred to as the phytoextraction process. This is followed by elevating REE concentrations into bio-ores via the enrichment phase. Ultimately, extraction is the final step to complete the phytomining pathway for reclaiming REEs in brownfield land. [Display omitted] • Analyzing the price and market of REEs to identify the demand side of phytomining. •Summarizing the studies regarding phytoextraction and phytomining of REEs. •Scrutinizing the enrichment and extraction approaches of REEs. •Focusing on the extraction of REEs from biomass and incineration solid remains. •Evaluating the viability of REE phytomining from the technical and economical perspectives. [ABSTRACT FROM AUTHOR]

Sample preparation is of vital importance for proteomic analysis because of the high complexity of biological samples. The rapid development of novel nanomaterials with various compositions, morphologies, and proper surface modifications provides a category of powerful tools for the sample preparation for protein analysis. In this paper, we have summarized recent progresses for the applications of novel nanomaterials in sample preparation for the analysis of proteomes, especially for phosphoproteomes, glycoproteomes, and peptidoms. Several kinds of novel nanomaterials were also discussed for their use in other kinds of proteomics analysis. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

Food has been industrially fortified (or enriched) with vitamins and minerals for 90 years. Cereal grains, specifically wheat flour, maize flour and rice, are good vehicles for fortification because they are available in large amounts in most countries around the world. The purpose of this paper is to review the evidence for the public-health impact of fortification, describe challenges to worldwide fortification, and list tools that are available to aid in the design, monitoring and evaluation of food-fortification programs. There is strong and consistent evidence that folic-acid fortification of cereal grains improves serum folate and reduces neural tube defects, and that iron-fortification increases serum ferritin levels. The results for hemoglobin are equivocal: some studies report increases, others decreases, and others no change in hemoglobin after cereal-grain fortification commenced with several nutrients. The two main challenges to worldwide fortification are that many countries that could benefit from cereal-grain fortification do not undertake this public-health intervention, and that few countries consistently report monitoring or evaluation data that suggests they are keeping track of the implementation of fortification. Finally, to overcome these challenges, many tools are available or under development to help with the design, monitoring and evaluation of food-fortification programs. [ABSTRACT FROM AUTHOR]

Biocement based on microbially induced carbonate precipitation has become a new construction material for soil cementation. One of the key elements of biocement is urease-producing bacteria (UPB). This paper presents a new method to enrich UPB from waste activated sludge (WAS) under a non-sterile condition so the cultivation of bacteria can be carried out in large-scale for field applications. Using this method, the cost of bacteria cultivation can be reduced by up to 30%. The UPB culture enriched using this method is highly urease active and capable of hydrolyzing urea for calcium carbonate precipitation. After treating sand columns using biocement made of this type of UPB via equal molar cementation solution of 2 M, the unconfined compressive strength of the sand increased to 2.7 MPa and the permeability reduced to a level of 10−6 m/s. These results are comparable with those obtained for biotreatment using pure UPB. Thus, the UPB cultivating method introduced in this paper has the potential to reduce the cost of soil improvement without compromising the performance. • UPB is enriched from WAS by using selective conditions. • The enrichment of UPB is under non-sterile condition. • Sterilization takes up 30% of total cost for MICP process. • The enriched UPB is capable to do soil improvement. [ABSTRACT FROM AUTHOR]

It has been reported that more than 90% of cancer patients are died from cancer metastasis. Circulating tumor cells (CTCs) could detach from solid tumors to form new lesions via blood transport and play an important role in cancer metastasis and progression. As part of the liquid biopsy, the investigation and analysis toward CTCs are of great importance for prognosis assessment and tumor precision medical treatment. Unfortunately, the enrichment of circulating tumor cells has been a huge challenge due to the fact that CTCs are very rare and vulnerable. Thus, a number of effective strategies have been developed for the enrichment of CTCs. This paper discusses the advantages and disadvantages of label-free and label-based methods commonly used in the isolation of CTCs. In particular, we systematically review the most recent advances in the combination of microfluidic chips and biosensing for the enrichment of circulating tumor cells. Finally, we put forward the current barriers that need to be overcome and developmental trends in the CTCs research. [ABSTRACT FROM AUTHOR]

This paper presents numerical simulations of transient dynamic fracture behaviors and quasi-static crack growth in cracked functionally graded composites using the enhanced extended consecutive-interpolation quadrilateral element (XCQ4). The mechanical properties of functional composites are assumed to vary continuously in spatial coordinates. In terms of XCQ4 modeling, only one function of ramp type (either linear, quadratic or cubic order), alternatively to the usual four branch functions, is used as crack-tip enrichment, somewhat reducing the complexity in implementation. Another advantage of employing the ramp function, which is not derived from asymptotic solutions, lies in the less additional degrees of freedom (DOFs) required for the branch functions, saving the computational effort. The merits of the developed approach are demonstrated through our numerical experiments, which are devoted to both dynamic loading with stationary cracks and quasi-static crack growth simulation in FGMs composites. The accuracy of the developed model is verified by comparing the computed results with respect to reference solutions derived from analytical solution, other numerical methods, and experimental data. • Transient dynamic fracture parameters in cracked FGM composites is studied using the XCQ4 element. • Quasi-static crack growth in FGM composites using the XCQ4 is also analyzed. • High accuracy in comparison with other numerical approaches is obtained. • The ramp function is used as crack tip-enrichment in inhomogeneous materials like FGM. • The ramp function requires less additional DOFs than the ordinary branch functions. [ABSTRACT FROM AUTHOR]

During the last decade, biochars have been considered as attractive and eco-friendly materials with various applications including wastewater treatment, energy production and soil amendments. However, the important nitrogen losses during biochars production using the pyrolysis process have limited their potential use in agriculture as biofertilizer. Therefore, it seems necessary to enrich these biochars with nitrogen sources before their use in agricultural soils. This paper is the first comprehensive review on the assessment of biomass type and the biochars' properties effects on N recovery efficiency from aqueous solutions as well as its release and availability for plants when applying the N-enriched chars in soils. In particular, the N recovery efficiency by raw biochars versus the type of the raw feedstock is summarized. Then, correlations between the adsorption performance and the main physico-chemical properties are established. The main mechanisms involved during ammonium (NH 4 –N) and nitrates (NO 3 –N) recovery process are thoroughly discussed. A special attention is given to the assessment of the biochars physico-chemical modification impact on their N recovery capacities improvement. After that, the application of these N-enriched biochars in agriculture and their impacts on plants growth as well as methane and nitrous oxide greenhouse gas emissions reduction are also discussed. Finally, the main future development and challenges of biochars enrichment with N from wastewaters and their valorization as biofertilizers for plants growth and greenhouse gas (GHG) emissions reduction are provided. This systematic review is intended to promote the real application of biochars for nutrients recovery from wastewaters and their reuse as eco-friendly fertilizers. [Display omitted] • Biochars have low nitrogen contents which limit their use in agriculture. • Biochars hybrid modifications allow high recovery yields of ammonium and nitrates. • N-laden biochars promote the seedling stage and the vegetative growth. • N-enriched biochars significantly reduce the N 2 O and CH 4 emissions. • N-enriched biochars promote sustainability and circular economy concepts. [ABSTRACT FROM AUTHOR]

Abstract: This paper describes a convenient and sensitive methodology for Salmonella Typhimurium (S. Typhimurium) detection in microfluidic channels using positive dielectrophoresis (pDEP) driven on-line enrichment and fluorescent nanoparticles label. In the protocol, S. Typhimurium was first incubated with the anti-S. Typhimurium antibody conjugated RuBpy-doped fluorescent nanoparticles (Ab S. Typhimurium-FNPs). After incubation, the reacted mixture of S. Typhimurium and Ab S. Typhimurium-FNPs bioconjugates was directly introduced into the pDEP-based microfluidic system. On applying an AC voltage to the electrodes in a pDEP frequency region, the FNPs labeled S. Typhimurium cells moved to the electrodes and accumulated in the electrode gap, while the free Ab S. Typhimurium-FNPs were flowed away. The enriched FNPs labeled S. Typhimurium were detected by fluorescence microscopy at the focused detection areas. This new method constructed a simultaneous on-line enrichment and detection platform, eliminated the separation and washing-out steps usually required for FNPs label involved bioassay. Utilizing signal amplification effects produced by pDEP on-line enrichment and FNPs label, the detection limit for S. Typhimurium in deionized water is 56 colony forming units per milliliter (cfu/mL). We applied the established approach to detecting artificially contaminated bottled mineral water samples; S. Typhimurium of 110cfu/mL was accurately detected. It is believed that the proposed method will find wide applications in biomedicine fields demanding higher sensitive bacterial identification. [Copyright &y& Elsevier]

Background Virtual screening in the form of similarity rankings is often applied in the early drug discovery process to rank and prioritize compounds from a database. This similarity ranking can be achieved with structural similarity measures. However, their general nature can lead to insufficient performance in some application cases. In this paper, we provide a link between ranking-based virtual screening and fragment-based data mining methods. The inclusion of binding-relevant background knowledge into a structural similarity measure improves the quality of the similarity rankings. This background knowledge in the form of binding relevant substructures can either be derived by hand selection or by automated fragment-based data mining methods. Results In virtual screening experiments we show that our approach clearly improves enrichment factors with both applied variants of our approach: the extension of the structural similarity measure with background knowledge in the form of a hand-selected relevant substructure or the extension of the similarity measure with background knowledge derived with data mining methods. Conclusion Our study shows that adding binding relevant background knowledge can lead to significantly improved similarity rankings in virtual screening and that even basic data mining approaches can lead to competitive results making hand-selection of the background knowledge less crucial. This is especially important in drug discovery and development projects where no receptor structure is available or more frequently no verified binding mode is known and mostly ligand based approaches can be applied to generate hit compounds. [ABSTRACT FROM AUTHOR]

Near infrared spectroscopy (NIRS) has been proved to be a powerful analytical tool in different fields. However, because of the low sensitivity in near infrared region, it is a significant challenge to detect trace analytes with normal NIRS technique. A novel enrichment technique called fluidized bed enrichment has been developed recently to improve sensitivity of NIRS which allows a large volume solution to pass through within a short time. In this paper, fluidized bed enrichment method was applied in the determination of trace dimethyl fumarate in milk. Macroporous styrene resin HZ-816 was used as adsorbent material, and 1 L solution of dimethyl fumarate was run to pass through the material for concentration. The milk sample was pretreated to remove interference matters such as protein, fat, and then passed through the material for enrichment; after that, diffuse reflection NIR spectra were measured for the analyte concentrated on the material directly without any elution process. The enrichment and spectral measurement procedures were easy to operate. NIR spectra in 900-1,700 nm were collected for dimethyl fumarate solutions in the concentration range of 0.506-5.060 μg/mL and then used for multivariate calibration with partial least squares (PLS) regression. Spectral pretreatment methods such as multiplicative scatter correction, first derivative, second derivative, and their combinations were carried out to select the optimal PLS model. Root mean square error of cross-validation calculated by leave-one-out cross-validation is 0.430 μg/mL with ten PLS factors. Ten samples in an independent test set were predicted by the model with the mean relative error of 5.33 %. From the results shown in this work, it can be concluded that the NIR technique coupled with on-line enrichment method can be expanded for the determination of trace analytes, and its applications in real liquid samples like milk and juice may also be feasible. [ABSTRACT FROM AUTHOR]

We open this Special Feature on stable isotopes in mammalian research with a beginner's guide, an introduction to the novice and a refresher to the well-versed. In this guide we provide the background needed to understand the more advanced papers that follow. We describe the basic principles of isotopic fractionation and discrimination, briefly explain the processes that govern isotopic incorporation into animal tissues, list some innovative studies, and provide cautionary notes and caveats. In addition to discussing the uses of natural abundance we present the concepts and applications of enriched isotopes and the potential combination of these 2 methodologies. We end with descriptions of analytical and conceptual developments that we believe will be cardinal to the future of isotopic analyses in mammalian research. [ABSTRACT FROM AUTHOR]

We consider a stoichiometric population model of two producers and one consumer. Stoichiometry can be thought of as the tracking of food quality in addition to food quantity. Our model assumes a reduced rate of conversion of biomass from producer to consumer when food quality is low. The model is open for carbon but closed for nutrient. The introduction of the second producer, which competes with the first, leads to new equilibria, new limit cycles, and new bifurcations. The focus of this paper is on the bifurcations which are the result of enrichment. The primary parameters we vary are the growth rates of both producers. Secondary variable parameters are the total nutrients in the system, and the producer nutrient uptake rates. The possible equilibria are: no-life, one-producer, coexistence of both producers, the consumer coexisting with either producer, and the consumer coexisting with both producers. We observe limit cycles in the latter three coexistence combinations. Bifurcation diagrams along with corresponding representative time series summarize the behaviours observed for this model. [ABSTRACT FROM PUBLISHER]