Search

A phytochemical investigation onEuphorbia alatavicaBoiss resulted in the isolation of nine compounds, including two new ones, alatavolide and alatavoic acid(1–2). Chemical structures of these compounds were established on the basis of 1D, 2D NMR, and HR-MS techniques, and by comparison with data reported in the literature. Compounds1,2,4,6,8,and9were screened for cytotoxicity using the MTT assay. Among these compounds, the new compound2showed moderate cytotoxicity against Hela, MCF-7 and A549 cell lines (IC50values of 16.4 ± 3.2, 14.5 ± 2.8, 22.3 ± 3.1 μM, respectively), while the known compound8exhibited the most potent cytotoxicity with the IC50values of 6.5 ± 3.1, 1.9 ± 0.9, 8.6 ± 3.5 μM, respectively. [ABSTRACT FROM AUTHOR]

A total synthesis of (±)-cis-5-hydroxycalamenene 1 has been achieved from tetralone 5, which in turn was prepared from 5-methoxy-α-tetralone 3. Grignard reaction of compound 5 with isopropylmagnesium chloride, followed by dehydration and aromatization, provided the substituted naphthalene 7 whose conversion to (±)-cis-5-hydroxycalamenene 1 was accomplished by demethylation, formylation, and hydrogenation. [ABSTRACT FROM AUTHOR]

Two new triterpenoid saponins, named cernuaside C and D, have been isolated from Pulsatilla cernua (Thunb.) Bercht. et Opiz. The structures of the two new triterpenoid saponins were elucidated as 3-O-β-D-xylopyranosyl(1 → 2)-[α-L-rhamnopyranosyl(1 → 3)]-α-L-arabinopyranosyl oleanolic acid 28-α-L-rhamnopyranosyl(1 → 4)-β-D-glucopyranosyl(1 → 6)-β-D-glucopyranoside. (1) and 3-O-α-L-arabinopyranosyl (1 → 3)-α-L-rhamnopyranosyl(1 → 2)-α-L-arabinopyranosyl oleanolic acid 28-O-β-D-glucopyranoside (2) by 1D, 2D-NMR techniques, ESI-MS analysis as well as chemical methods. [ABSTRACT FROM AUTHOR]