1. Cryptotanshinone Suppressed Inflammatory Cytokines Secretion in RAW264.7 Macrophages through Inhibition of the NF-κB and MAPK Signaling Pathways.
- Author
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Tang, Shu, Shen, Xiao-Yan, Huang, He-Qing, Xu, Suo-Wen, Yu, Yang, Zhou, Chang-Hua, Chen, Shao-Rui, Le, Kang, Wang, Yu-Hua, and Liu, Pei-Qing
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CYTOKINES , *MACROPHAGES , *HERBS , *ANTIOXIDANTS , *ANTI-inflammatory agents , *POLYSACCHARIDES , *ENZYME-linked immunosorbent assay , *TUMOR necrosis factors , *PHOSPHORYLATION , *INTERLEUKIN-6 - Abstract
Cryptotanshinone (CTS), a major constituent extracted from the medicinal herb Salvia miltiorrhiza Bunge, has well-documented antioxidative and anti-inflammatory effects. In the present study, the pharmacological effects and underlying molecular mechanisms of CTS on lipopolysaccharide (LPS)-induced inflammatory responses were investigated. By enzyme-linked immunosorbent assay, we observed that CTS reduced significantly the production of proinflammatory mediators (tumor necrosis factor-α and interleukin-6) induced by LPS in murine macrophage-like RAW264.7 cells. Mechanistically, CTS inhibited markedly the phosphorylation of mitogen-activated protein kinases (MAPKs), including ERK1/2, p38MAPK, and JNK, which are crucially involved in regulation of proinflammatory mediator secretion. Moreover, immunofluorescence and western blot analysis indicated that CTS abolished completely LPS-triggered nuclear factor-κB (NF-κB) activation. Taken together, these data implied that NF-κB and MAPKs might be the potential molecular targets for clarifying the protective effects of CTS on LPS-induced inflammatory cytokine production in macrophages. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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