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Objective To investigate the expression of microRNA-363-3p （miR-363-3p） and sex-determining region Y-box protein 4（SOX4） mRNA in the non-small-cell lung cancer （NSCLC） tissues and their effects on the epithelmesenchymal transformation （EMT). Methods Cancer tissues and adjacent tissues of 84 patients with NSCLC who underwent surgical resection were collected. Real-time fluorescence quantitative PCR was used to detect miR-363-3p and SOX4 mRNA in the cancer tissues and adjacent tissues, and immunohistochemistry was used to detect the E-cadherin and Vimentin expression in the cancer tissues and adjacent tissues. The mRNA expression levels of miR-363-3p and SOX4 in the cancer tissues of patients with different pathological characteristics were compared. Spearman's rank correlation test was used to analyze the correlation between miR-363-3p, SOX4 mRNA expression and E-cadherin and Vimentin in the cancer tissues. The interaction of miR-363-3p and SOX4 in EMT process was analyzed using relative excess risk due to interaction （RERI), attributable proportion due to interaction （AP） and interaction index （SI）. Results The expression of miR-363-3p in the cancer tissues was lower than that in the adjacent tissues, and the expression of SOX4 mRNA was higher than that in the adjacent tissues （both P<0. 05）. The mRNA expression levels of miR-363-3p and SOX4 in the cancer tissues were not related to gender, age, smoking history or tumor diameter （all P>0. 05), but was related to differentiation degree and TNM stage (all P<0. 05). The positive expression rate of E-cadherin in the cancer tissues was lower than that in the adjacent tissues, and the positive expression rate of Vimentin was higher than that in the adjacent tissues （both P<0. 05）. The miR-363-3p was positively correlated with the expression of E-cadherin (r=0. 491，P<0. 05), and was negatively correlated with the expression of Vimentin （r=–0. 506，P<0. 05). SOX4 mRNA was negatively correlated with E-cadherin expression (r=–0. 527, P<0. 05), and was positively correlated with Vimentin expression （r=0. 463，P<0. 05). The miR-363-3p and SOX4 in the cancer tissues had negative interactions with EMT process （RERI=29. 639， AP=70. 91, SI=3. 656). Conclusion The abnormal expression of miR-363-3p and SOX4 mRNA in NSCLC tissues is related to EMT, and the two have negative interaction with EMT process. [ABSTRACT FROM AUTHOR]

环状RNAs高度稳定，且不易被核酸外切酶降解，能够存在于外泌体和血液中。其在食管鳞癌、胃癌、结 直肠癌、肝细胞癌、胰腺导管癌中表达异常，具有微小RNA海绵、参与蛋白翻译、调控基因转录及剪切等功能，通过 多种信号通路在tl述消化系肿瘤发生、发展及转移中发挥重要调控作用，并且可以作为分子标志物用于消化道肿 瘤的诊断和预后判断。 [ABSTRACT FROM AUTHOR]

Objective To investigate the expression and mutations of DNA mismatch repair ( MMR) of Lynch syn-drome in the members of a Chinese Hui family. Methods The propositus was male, 37 years old, the forth generation, Hui nationality, had rectal poorly differentiated adenocarcinoma. There were six generations existing ( Hui nationality), and a total of 14 patients with colorectal cancer were found including 7 living cases. The peripheral blood and tumor tissue DNA was collected from 6 cases of patients and 5 healthy controls. The microsatellite instability ( MSI ) was detected through DNA sequencing, the expression of MMR protein was measured using immunohistochemistry, and MMR gene muta-tions was detected by PCR amplification. Results The MSI detection of tumor tissues in the family members demonstrated MSI-H.The immunohistochemistry showed that MLH1 protein was negative. Two new missense mutation sites ( c.264G>T and c.265G>T) in the first exon of MLH1 gene were found in 6 patients and 1 normal individual, and c.264G>T mu-tation led to early termination of MLH1 protein translation on this site. Conclusion There is MLH1 mutation in Hui family with Lynch syndrome, that is the missense muntation site c.264G>T in the first exon of MLH1 gene. [ABSTRACT FROM AUTHOR]

目的 系统评价质子泵抑制剂(PPI)使用与胃底腺息肉发病的关系. 方法 计算机检索CBM、CNKI、WANFANG Data、VIP Data、PubMed、Embase数据库中PPI使用与胃底腺息肉发病关系的相关文献,检索时间截至2015年2月20日. 由2名研究者根据纳入标准与排除标准独立筛选文献,采用纽卡斯尔-渥太华量表标准评价纳入文献质量,运用RevMan5.3及STATA11.0软件进行Meta分析. 结果 共纳入6个队列研究,研究对象34 701例,其中胃底腺息肉1 513例. Meta分析结果显示,PPI使用与胃底腺息肉的发病存在关联(RR=2.46,95% CI为1.37~4.43,P<0.01);亚组分析显示,PPI服用1年以上者胃底腺息肉发生率高于不足1年者(RR=3.12,95%为CI为1.72~5.67,P<0.05);且随着PPI服用时间延长,二者相关性有增加趋势. 敏感性分析表明,本Meta分析结果稳定可靠,Egger检验提示可能存在发表偏倚. 结论 PPI使用可能与胃底腺息肉发病有关. [ABSTRACT FROM AUTHOR]

目的：观察完整结肠系膜切除术（CME）治疗右半结肠癌的短期效果及安全性。方法选择右半结肠癌患者96例，随机分为CME组和传统手术组各48例，比较两组手术时间、术中出血量、淋巴结清扫数目、患者住院天数及术后并发症发生情况。结果两组均完整地切除了癌灶和系膜组织。两组手术时间、术中出血量、住院天数及术后并发症发生率比较，P均＞0．05；CME组术中淋巴结清扫数目为（17．7±1．9）枚，传统手术组为（15．8±2．4）枚，P＜0．05。结论 CME可以完整地切除癌灶和系膜组织，实现淋巴结清扫的最大化，且不增加手术风险及术后并发症发生率。 [ABSTRACT FROM AUTHOR]