1. Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver.
- Author
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Zhang, Ding-Ding, Wang, Dan-Dan, Wang, Zhang, Wang, Yang-Bin, Li, Guo-Xi, Sun, Gui-Rong, Tian, Ya-Dong, Han, Rui-Li, Li, Zhuan-Jian, Jiang, Rui-Rui, Liu, Xiao-Jun, Kang, Xiang-Tao, and Li, Hong
- Subjects
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LIPID synthesis , *LIVER , *CHICKENS , *PHYSIOLOGICAL control systems , *ESTROGEN , *LUCIFERASES , *SEX hormones , *CHICKEN diseases - Abstract
Few studies have been conducted regarding the biological function and regulation role of gga-miR-221-5p in the liver. We compared the conservation of miR-221-5p among species and investigated the expression pattern of gga-miR-221-5p, validating the direct target genes of gga-miR-221-5p by dual luciferase reporter assay, the biological function of gga-miR-221-5p in the liver was studied by gga-miR-221-5p overexpression and inhibition. Furthermore, we explored the regulation of gga-miR-221-5p and its target genes by treatment with estrogen and estrogen antagonists in vivo and in vitro. The results showed that miR-221-5p was highly conserved among species, expressed in all tested tissues and significantly downregulated in peak-laying hen liver compared to pre-laying hen liver. Gga-miR-221-5p could directly target the expression of elongase of very long chain fatty acids 6 (ELOVL6) and squalene epoxidase (SQLE) genes to affect triglyceride and total cholesterol content in the liver. 17β-estradiol could significantly inhibit the expression of gga-miR-221-5p but promote the expression of ELOVL6 and SQLE genes. In conclusion, the highly conservative gga-miR-221-5p could directly target ELOVL6 and SQLE mRNAs to affect the level of intracellular triglyceride and total cholesterol. Meanwhile, 17β-estradiol could repress the expression of gga-miR-221-5p but increase the expression of ELOVL6 and SQLE, therefore promoting the synthesis of intracellular triglyceride and cholesterol levels in the liver of egg-laying chicken. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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