1. Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas.
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Burucúa, Mercedes M., Risalde, María A., Cheuquepán, Felipe A., Quintana, Silvina, Pérez, Sandra E., Cantón, Germán J., Moore, Dadin P., Odeón, Anselmo C., Agulló-Ros, Irene, Scioli, María Valeria, Barbeito, Claudio, Morrell, Eleonora L., and Marín, Maia S.
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PROTEIN expression , *PORCINE reproductive & respiratory syndrome , *TUMOR necrosis factors , *NITRIC-oxide synthases , *PLACENTA , *CYCLOOXYGENASE 2 , *INFLAMMATORY mediators - Abstract
Bovine alphaherpesvirus type 1 (BoAHV-1) is associated with respiratory and reproductive syndromes. Until present the immunologic mechanisms involved in BoAHV-1 abortion are partially known. We studied key elements of the innate immune response in the placentas and fetal lungs from cattle experimentally-inoculated with BoAHV-1. These tissues were analyzed by histopathology. Furthermore, virus identification was performed by qPCR and the expression of the inflammatory cytokines such as tumor necrosis factor-alpha, interleukin 1-alpha and inflammatory mediators like inducible nitric oxide synthase and cyclooxeganse-2 was evaluated by immunohistochemistry. The viral transplacental infection was confirmed by the detection of BoAHV-1 by qPCR in the placenta and fetal organs, which revealed mild inflammatory lesions. Inducible nitric oxide synthase immunolabelling was high in the lungs of infected fetuses and placentas, as well as for tumor necrosis factor-alpha in the pulmonary parenchyma and cyclooxeganse-2 in fetal annexes. However, the expression of interleukin 1-alpha was weak in these organs. To our knowledge, this is the first study that provides strong evidence of an early immune response to BoAHV-1 infection in the conceptus. Advances in the knowledge of the complex immunological interactions at the feto-maternal unit during BoAHV-1 infection are needed to clarify the pathogenesis of abortion. • BoAHV-1 transplacental infection causes an early immune response at the feto-maternal unit. • BoAHV-1 induces protein expression of iNOS in fetal lung and placenta. • BoAHV-1 induces protein expression of TNFα in fetal lungs and COX-2 in the placenta. • The expression of pro-inflammatory mediators in the placenta by BoAHV-1 might trigger the abortion or control the infection. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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