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1. In vitro and in vivo antioxidant effects of the ethanolic extract of Swertia chirayita

Author

Chen, Yue, Huang, Bo, He, Jingsheng, Han, Li, Zhan, Yichao, and Wang, Youwei

Subjects
*HEPATOTOXICOLOGY, *MEDICINAL plants, *ALTERNATIVE medicine, *ANALYSIS of variance, *ANIMAL experimentation, *ANTIOXIDANTS, *BIOLOGICAL assay, *BIOPHYSICS, *DRUG toxicity, *ENZYMES, *FLAVONOIDS, *GLUTATHIONE, *HISTOLOGICAL techniques, *KIDNEYS, *LIVER, *RESEARCH methodology, *MICE, *POLYPHENOLS, *RESEARCH funding, *STATISTICS, *SUPEROXIDE dismutase, *MALONDIALDEHYDE, *PLANT extracts, *DATA analysis, *OXIDATIVE stress, *PREVENTION
Abstract

Abstract: Ethnopharmacological relevance: Swertia chirayita, a medicinal herb endemic to the Tibetan region, is used as a special remedy for liver disorders. The hepatoprotective activity of its plant extracts has been associated with its antioxidant activity. This paper aims to investigate the in vitro and in vivo antioxidant effects of Swertia chirayita extracts (SCE). Materials and methods: Antioxidant ability of Swertia chirayita was investigated by employing several established in vitro methods. In vivo antioxidant activity was tested against CCl4-induced toxicity in mice. The levels and activities of malondialdehyde (MDA) and antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), were then assayed using standard procedures. Results: SCE exhibited strong antioxidant ability in vitro. The liver and kidney of CCl4-intoxicated animals exhibited a significant (p <0.001) decrease in SOD, CAT, and GSH levels. Additionally, these organs exhibited a significant (p <0.001) increase in MDA level. CCl4 did not exhibit toxicity on mice treated with SCE and Vitamin E. The effects of Swertia chirayita (three dosages) were comparable to those of Vitamin E, except in MDA level in the liver and GSH level in the kidney (p <0.05). Conclusion: This study suggests that the ethanolic extract of Swertia chirayita possesses in vitro and in vivo antioxidant effects. This supports the traditional use of Swertia chirayita in Tibetan medicine to cure liver diseases. [Copyright &y& Elsevier]

Published
2011
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2. Antinociceptive and anti-inflammatory activities of Phlomis umbrosa Turcz extract

Author

Shang, Xiaofei, Wang, Jinhui, Li, Maoxing, Miao, Xiaolou, Pan, Hu, Yang, Yaoguang, and Wang, Yu

Subjects
*PAIN, *PREVENTIVE medicine, *MEDICINAL plants, *ALTERNATIVE medicine, *ANALYSIS of variance, *ANIMAL experimentation, *ANTI-inflammatory agents, *BIOPHYSICS, *COMPUTER software, *DRUG toxicity, *HIGH performance liquid chromatography, *RESEARCH methodology, *MICE, *RESEARCH funding, *STATISTICAL hypothesis testing, *STATISTICS, *T-test (Statistics), *THIN layer chromatography, *PLANT extracts, *DATA analysis, *PHARMACODYNAMICS
Abstract

Abstract: Phlomis umbrosa Turcz has been used as the traditional medicine for thousands of years in China. In this paper, the acetic acid-induced writhing test, the hot plate test, the carrageenan-induced paw edema test, the xylene-induced ear swelling test, and the acetic acid-induced Evans blue leakage and leukocyte infiltration test were used to investigate the antinociceptive and anti-inflammatory activities of the aqueous extract of this plant (25, 50 and 100mg/kg i.p.). Good dose-dependent effects were obtained in most of these tests, except in the hot plate test and the acetic acid-induced Evans blue leakage test. TLC and HPLC analyses showed iridoid glucosides were the main compositions of this extract. These findings suggested that the aqueous extract of P. umbrosa has significant antinociceptive and anti-inflammatory activities. [Copyright &y& Elsevier]

Published
2011
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