1. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells.
- Author
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Xiao-Tian Zhang, Chun-Jiang Yu, Jian-Wei Liu, Yan-Ping Zhang, Chao Zhang, Chen-Xue Song, Jing-Shu Xie, Jing-Ying Sai, Jing-Tong Zheng, and Fang Wang
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REACTIVE oxygen species , *CELL physiology , *COMPARATIVE studies , *STATISTICAL correlation , *CYTOKINES , *ENZYME-linked immunosorbent assay , *GENE expression , *INFLAMMATION , *INSULIN resistance , *CHINESE medicine , *TYPE 2 diabetes , *POLYMERASE chain reaction , *T-test (Statistics) , *WESTERN immunoblotting , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *IN vitro studies - Abstract
We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P < 0.001); however, QJJ restored HepG2 cell survival (P < 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (all P < 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (all P < 0.05). Furthermore, high-dose QJJ reduced both TNF-α and IL-6 levels as compared to the IR group (all P < 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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