1. Cytotoxicity of Cymbopogon citratus (DC) Stapf fractions, essential oil, citral, and geraniol in human leukocytes and erythrocytes.
- Author
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Mendes Hacke, Ana Carolina, D'Avila da Silva, Fernanda, Lima, Dhésmon, Rebuglio Vellosa, José Carlos, Teixeira Rocha, João Batista, Marques, Jacqueline Aparecida, and Pereira, Romaiana Picada
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MEDICINAL plants , *ESSENTIAL oils , *TERPENES , *SOLVENTS , *LEUCOCYTES , *ANIMAL experimentation , *HEMOLYSIS & hemolysins , *CELL membranes , *CELL physiology , *PHYTOCHEMICALS , *CRUSTACEA , *ELECTRON microscopy , *CELL survival , *LEAVES , *ACETIC acid , *DESCRIPTIVE statistics , *DOSE-effect relationship in pharmacology , *PLANT extracts , *ERYTHROCYTES , *ETHANOL , *CYTOTOXINS - Abstract
Our recently published paper demonstrated that ethyl acetate fractions obtained from Cymbopogon citratus (DC.) Stapf (C. citratus) leaves, which are consumed as infusion in folk medicine due to their therapeutic properties, are rich in polyphenols and exhibit promising antioxidant activity by acting through different mechanisms in vitro. However, studies regarding the toxicity of these fractions are necessary to investigate their safe use in future biomedical applications. This study aimed to investigate the toxicity of ethyl acetate (obtained in acidic and basic conditions and after the essential oil removal from the leaves) and chloroform fractions, essential oil, and its pure constituents, citral and geraniol. The toxicity of C. citratus samples was evaluated by using Artemia salina (A. salina) and human blood cells (leukocytes and erythrocytes). The A. salina lethality assay demonstrated that C. citratus fractions were moderately toxic with LC 50 values ranging from 146.12 to 433.15 μg mL−1, whereas the essential oil and isolated compounds were highly toxic with LC 50 lower than 100 μg mL−1. Leukocyte viability decreased after incubation in the presence of the fractions obtained after the essential oil removal from the plant leaves, as well as in the presence of essential oil, citral and geraniol. The same samples increased the osmotic fragility of erythrocytes, and field emission gun scanning electron microscopy (FESEM) analysis revealed significant changes in cell morphology. Interestingly, our results suggest that the previous removal of essential oil from plant leaves facilitated the extraction of cytotoxic compounds from C. citratus. It was demonstrated that C. citratus ethyl acetate and chloroform fractions, essential oil, as well citral and geraniol were considered toxic to A. salina , cytotoxic to human blood cells and showed to induce alterations in the erythrocyte membrane at higher concentrations. These fractions will be further investigated to identify the phytochemicals involved in the observed cytotoxic effects and explored using in vivo models. [Display omitted] • Cymbopogon citratus samples and isolated compounds were toxic for Artemia salina. • Citral, geraniol and essential oil increased osmotic fragility in human erythrocytes. • FESEM analysis showed that some samples changed erythrocytes morphology. • The removal of essential oil from leaves enhanced the extraction of cytotoxic compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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