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Background: Gap junction remodeling is an important cause of ventricular arrhythmia in heart failure. However, it remains unclear whether renal denervation (RDN) regulates gap junction remodeling in heart failure. To explore the effect of RDN on gap junction remodeling in dogs with high-pacing-induced heart failure. Methods: Fifteen dogs were randomly divided into control (n=5), heart failure (HF) (n=5), and RDN+HF (n=5) group. A high-pacing-induced-heart failure model was established using rapid right ventricular pacing for 4 weeks. The RDN+HF group underwent surgical and chemical ablation of both renal arteries before 4 weeks rapid right ventricular pacing. After 4 weeks, echocardiography, High-Performance Liquid Chromatography-Mass Spectrometry test for norepinephrine and epinephrine, and pathological analysis were performed in the above 3 groups. Further, immunohistochemical staining was used to detect tyrosine hydroxylase, ChaT, connexin 43 (Cx43), and connexin 40 (Cx40). Connexin 43 and Cx40 expression was detected by western blotting. Transmission electron microscopy was used to observe the gap junction. Results: Compared to the control group, myocardial fibrosis and sympathetic hyperactivity were observed in the HF group. Immunohistochemical staining and western blotting showed that Cx40 expression and Cx43 expression was significantly reduced in the HF group. Compared with the HF group, the RDN+HF group showed reduced sympathetic hyperactivity, Cx40 expression, Cx40/Cx43 ratio, and increased Cx43 expression. Conclusion: Renal denervation alleviates gap junction remodeling in high-pacinginduced heart failure dogs. [ABSTRACT FROM AUTHOR]

A meta-analysis and trial sequential analysis was conducted to evaluate the risk of COVID-19 in patients with atopic dermatitis (AD). Seven studies were included in the analysis, which showed that the risk of COVID-19 in AD patients was similar to that of control subjects. The analysis also suggested that AD patients may have better hygiene habits and reduced social interaction, which could contribute to a lower infection risk. However, the impact of AD on the severity of COVID-19 was not analyzed. It is recommended that AD patients continue to practice prevention measures and consider teledermatology for follow-up visits. [Extracted from the article]

In this paper, a metamaterial electromagnetic energy harvester constructed via the capacitive loading of metal circular split rings is presented. Each energy-harvesting cell is loaded with a resistance that imitates the input impedance of a rectifier circuit. Specifically, the metamaterial energy harvester has high selective harvesting for left- and right-handed circularly polarized waves. Here, the energy absorption is mostly induced by the resistive load; thus, effective energy harvesting can be achieved. Moreover, the proposed energy harvester exhibits a high-efficiency harvesting for right-handed circularly polarized waves over a wide range of incident angles. Further, a transmission line model is adopted to interpret the energy harvesting mechanism, which shows that a good impedance matching and low dielectric loss can further enhance the harvesting efficiency. To demonstrate the design, a 15 15 unit-cell prototype is fabricated and measured, and the measured results reasonably agree with the simulated ones. [ABSTRACT FROM AUTHOR]

Our aim was to obtain details of the condition of the scimitar-horned oryx (Oryx dammah) and to elucidate the gut microbial diversity of captive individuals of this species. For the first time, 16S rRNA barcoding was used to characterise the faecal microbiota of five captive scimitar-horned oryx individuals. A total of 15 prokaryotic phyla were identified in the five samples. Including Firmicutes (53.40-72.01%), Bacteroidetes (12.94-23.72%) and Proteobacteria (1.03-31.74%), accounting for > 96% of all the sequences. At the family level, there were < 3.5% unclassified sequences. In conclusion, these data revealed similarities and differences in gut microbial diversity across the scimitar-horned oryx individuals. These observations advance the current understanding of the bacterial ecosystems in these endangered animals under captivity. [ABSTRACT FROM AUTHOR]

Ahigh performance metamaterial device for electromagnetic energy harvesting is presented. The unit cell of the metamaterial device consists of four identical omega ring resonators arranged in rotational symmetry, and each omega ring is loaded with a 50 Ω resistor that mimics the input impedance of a rectifier circuit. A proof-of-concept prototype is designed for operation at the frequency of 5.8 GHz. Here, the efficient energy harvesting is validated by the energy dissipated in each resistor. Based on the full absorption concept, the metamaterial device achieves a harvesting efficiency up to 93.1% under normal incidence. In addition, the energy harvester shows polarization independent and wide-angle incident frequency responses with good harvesting characteristics over the entire operating range. The surface current distribution is analyzed to gain an insight into the energy harvesting mechanism. Moreover, the harvester prototype is manufactured and measured, and the simulated and measured results are in good agreement. Due to the enhanced electromagnetic energy harvesting efficiency, the proposed device is a good candidate for microwave power transmission. [ABSTRACT FROM AUTHOR]

In this study, the design, realization, and characterization of an ultrathin triple-band polarization-insensitive wide-angle metamaterial absorber are reported. The metamaterial absorber comprises a periodic array of modified six-fold symmetric snowflakeshaped resonators with strip spiral line load, which is printed on a dielectric substrate backed by a metal ground plane. It is shown that the absorber exhibits three distinct near-unity absorption peaks, which are distributed across C, X, Ku bands, respectively. Owing to the six-fold symmetry, the absorber is insensitive to the polarization of the incident radiation. In addition, the absorber shows excellent absorption performance over wide oblique incident angles for both transverse electric and transverse magnetic polarizations. Simulated surface current and field distributions at the three absorption peaks are demonstrated to understand the absorption mechanism. Particularly, the absorption modes come from the fundamental and high-order dipole resonances. Furthermore, the experimental verification of the designed absorber is conducted, and the measured results are in reasonable agreement with the simulated ones. The proposed ultrathin (∼0.018λ0, λ0 corresponding to the lowest peak absorption frequency) compact (0.168λ0×0.168λ0 corresponding to the area of a unit cell) absorber enables potential applications such as stealth technology, electromagnetic interference and spectrum identification. [ABSTRACT FROM AUTHOR]

Background: While desmosomal junctions and gap junction remodeling are among the arrhythmogenic substrates, the fate of desmosomal and gap junctions in high-pacinginduced heart failure remains unclear. This aim of this study was to determine the fate of desmosomal junctions in high-pacing-induced heart failure. Methods: Dogs were randomly divided into 2 equal groups, a high-pacing-induced heart failure model group (heart failure group, n = 6) and a sham operation group (control group, n = 6). Echocardiography and cardiac electrophysiological examination were performed. Cardiac tissue was analyzed by immunofluorescence and transmission electron microscopy. The expression of desmoplakin and desmoglein-2 proteins was detected by western blot. Results: A significant decrease in ejection fraction, significant cardiac dilatation, diastolic and systolic dysfunction, and ventricular thinning occurred after 4 weeks in high-pacinginduced dog model of heart failure. Effective refractory period action potential duration at 90% repolarization was prolonged in the heart failure group. Immunofluorescence analysis and transmission electron microscopy demonstrated connexin-43 lateralization accompanies desmoglein-2 and desmoplakin remodeling in the heart failure group. Western blotting showed that the expression of desmoplakin and desmoglein-2 proteins was higher in heart failure than in normal tissue. Conclusion: Desmosome (desmoglein-2 and desmoplakin) redistribution and desmosome (desmoglein-2) overexpression accompanying connexin-43 lateralization were parts of a complex remodeling in high-pacing-induced heart failure. [ABSTRACT FROM AUTHOR]

This article is a meta-analysis and trial sequential analysis that evaluates the risk of bone fractures in patients with atopic dermatitis (AD). The study includes four high-quality studies from different regions, with a total of 409,366 AD patients and 1,704,553 control subjects. The analysis shows that AD patients have an elevated risk of bone fractures compared to non-AD control subjects, with a risk ratio of 1.10. The study suggests that clinicians should be aware of the increased fracture risk in AD patients and further research is needed to understand the mechanisms behind this association. [Extracted from the article]

Starting from the transformation and upgrading of traditional culture, the optimization and development of traditional industries, and the innovation of traditional models, this paper used cultural entrepreneurship to trigger the thematic business engine, and explored Zhucheng City with its unique dinosaur culture. It proposed "Three-integration" rolling development mode to build Zhucheng into a new highland of cultural entrepreneurship and create new competitiveness under the background of agricultural and rural modernization development. This paper also explored from the perspective of multi-subject collaboration of cultural entrepreneurship. Government needs to establish rolling development pilot projects and promote their application; enterprises need to play the engine role of thematic business through the mode of "getting larger to help smaller ones"; the masses need to pay attention to the reaction of culture to the economy, so as to promote the wave of high-level promotion of cultural inheritance and urban-rural integration. [ABSTRACT FROM AUTHOR]

Metal mesh transparent electrodes present a promising alternative to Indium-Tin Oxide (ITO) due to their adjustable period and favourable trade-off between transmittance and conductivity. In this work, a template-free, non-high temperature hybrid additive manufacturing approach of the polyacrylonitrile (PAN)/Cu core--shell structure high-resolution metal mesh flexible transparent electrode (FTE) is proposed. The electric field-driven (EFD) 3D printing method was employed to print ultra-fine lines with widths as low as 1 μm. The composite-plating process combining electroless plating and electroplating has solved the structural defects of single deposition process. The prepared FTE has an excellent conductivity down to 1 Ω/sq and 89% light transmission (at 550 nm). Its exceptional mechanical properties and environmental stability make it suitable for diverse working environments. Even after undergoing 2000 bends at a radius of 3 mm, the resistance change rate remains as low as 1.4%. The resistance exhibits an approximately 8% change rate in the acid--base environment experiments conducted over 72 h. The flexible touch screen prepared with this FTE exhibits excellent writing performance in both flat and curved working scenarios. Furthermore, the significant potential of this FTE in the field of optoelectronics is effectively demonstrated through its exceptional luminescent performance in electroluminescent devices. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the association between IL-25, IL-33 and AD more generally. Methods: Databases, including PubMed, Web of Science, EMBASE, Scopus, CNKI and Sinomed were searched. Based on the criteria, publications were collected. The evaluation of study quality was through Newcastle-Ottawa Scale (NOS). Fixed or random effect model was selected according to the between-study heterogeneity to evaluate the association. The analysis procedure and the construction plots were using Review Manager 5.3 software. Results: Six studies were included. A total of 282 subjects were included from four studies to analyze the association between IL-25 and AD. The level of IL-25 was significantly elevated in AD patients, comparing with the control subjects (SMD = 0.89, 95% CI: 0.64, 1.14, p < 0.05). For IL-33, a total of 247 subjects were included from two studies, and the level of IL-33 was also significantly elevated in AD patients comparing to the control subjects (SMD = 0.49, 95% CI: 0.19, 0.80, p < 0.05). Conclusions: The serum levels of IL-25, IL-33 are elevated in AD patients of this study. The IL-25 and IL-33 are significantly associated with the risk of AD. Further studies with larger samples, in multiple countries and focused on different age groups are need. [ABSTRACT FROM AUTHOR]

Mutations in ATP13A2 cause Kufor-Rakeb syndrome, an autosomal recessive form of juvenile-onset atypical Parkinson's disease (PD). Recent work tied ATP13A2 to autophagy and other cellular features of neurodegeneration, but how ATP13A2 governs numerous cellular functions in PD pathogenesis is not understood. In this study, the ATP13A2-deficient mouse developed into aging-dependent phenotypes resembling those of autophagy impairment. ATP13A2 deficiency impaired autophagosome-lysosome fusion in cultured cells and in in vitro reconstitution assays. In ATP13A2-deficient cells or Drosophila melanogaster or mouse tissues, lysosomal localization and activity of HDAC6 were reduced, with increased acetylation of tubulin and cortactin. Wild-type HDAC6, but not a deacetylase-inactive mutant, restored autophagosome-lysosome fusion, antagonized cortactin hyperacetylation, and promoted lysosomal localization of cortactin in ATP13A2-deficient cells. Mechanistically, ATP13A2 facilitated recruitment of HDAC6 and cortactin to lysosomes. Cortactin overexpression in cultured cells reversed ATP13A2 deficiency-associated impairment of autophagosome-lysosome fusion. PD-causing ATP13A2 mutants failed to rescue autophagosome-lysosome fusion or to promote degradation of protein aggregates and damaged mitochondria. These results suggest that ATP13A2 recruits HDAC6 to lysosomes to deacetylate cortactin and promotes autophagosome-lysosome fusion and autophagy. This study identifies ATP13A2 as an essential molecular component for normal autophagy flux in vivo and implies potential treatments targeting HDAC6-mediated autophagy for PD. [ABSTRACT FROM AUTHOR]

Background: The wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, because it is well adapted to various ecological niches and their corresponding pathogen environments. Immunological competence is a crucial factor involved in adapting to a changing environment and fighting pathogen infection in animals. In this study, the peripheral blood transcriptome of wolves was generated via RNA-seq to advance understanding of the wolf immunome, with a special focus on the major histocompatibility complex class I (MHC I) and toll-like receptor (TLR) gene families, which are involved in pathogen recognition and defense. Results: The blood transcriptomic libraries of eight wolves originating from Tibet and Inner Mongolia were sequenced, and approximately 383 million reads were generated. Using a genome-guided assembly strategy, we obtained 123,851 unigenes, with a mean length of 845 bp and an N50 length of 1121 bp. On the basis of BLAST searches against the NCBI non-redundant protein database (Nr), a total of 36,192 (29.22%) unigenes were annotated. For functional classification, 24,663 unigenes were assigned to 13,016 Gene Ontology (GO) terms belonging to 51 sub-categories of the three main GO categories. Additionally, 7682 unigenes were classified into 6 Kyoto Encyclopedia of Genes and Genomes (KEGG) categories, in which the most represented functional sub-categories were signal transduction and the immune system, and 16,238 unigenes were functionally classified into 25 Eukaryotic Orthologous Groups (KOG) categories. We observed an overall higher ω (dN/dS) value at antigen-binding sites (ABSs) than at non-ABS regions as well as clear evidence of intergenic/intragenic recombination events at wolf MHC I loci. Additionally, our analysis revealed that carnivorous TLRs were dominated by purifying selection, with mean ω values at each TLR locus ranging from 0.173 to 0.527. However, we also found significant instances of positive selection that acted on several codons in pathogen recognition domains and were linked to species-specific differences in pathogen recognition. Conclusions: This study represents the first attempt to characterize the blood transcriptome of the wolf and to highlight the value of investigating the immune system. Balancing selection and recombination have contributed to the historical evolution of wolf MHC I genes. Moreover, TLRs in carnivores have undergone adaptive evolution against the background of purifying selection, and a high level of adaptive evolution was detected in the wolf TLR system. [ABSTRACT FROM AUTHOR]

Curcumin possesses strong anti-inflammatory, anti-rheumatoid and anti-oxidative activities, and has the potential to inhibit nuclear factor-κB (NF-κB) signaling. Cartilage damage in osteoarthritis (OA) is largely mediated by interleukin-1β (IL-1β) via activation of various transcription factors, including NF-κB and activator protein-1. The aim of the present study was to determine whether IL-1β induces matrix metalloproteinase-13 (MMP-13) expression and inhibits type II collagen expression, as well as to examine whether cell proliferation may be inhibited by curcumin through the inhibition of NF-κB signaling. The effects of curcumin were investigated in rat articular chondrocyte cell cultures treated with IL-1β in the presence or absence of curcumin or the NF-κB inhibitor pyrrolidine dithiocarbamate. Western blotting and reverse transcription-quantitative polymerase chain reaction were conducted to evaluate protein and mRNA expression levels of type II collagen, MMP-13, NF-κB inhibitor α (IκBα), phosphorylated-IκBα and NF-κB subunit p65/RelA. Western blotting and immunofluorescence were performed to examine the effects of curcumin on the expression, phosphorylation and nuclear translocation of NF-κB-associated proteins. The effects of curcumin on cell proliferation were evaluated by Cell Counting Kit-8 (CCK-8). Curcumin was demonstrated to inhibit the IL-1β-induced activation of NF-κB by suppressing IκBα phosphorylation and p65/RelA nuclear translocation. These events were associated with the downregulation of MMP-13 expression and the upregulation of type II collagen expression, both of which are considered to be NF-κB targets. CCK-8 assays revealed that co-treatment with curcumin resulted in increased proliferation in IL-1β-treated chondrocytes. These findings implicated curcumin as a naturally occurring anti-inflammatory agent for the treatment of OA via inhibition of NF-κB signaling. [ABSTRACT FROM AUTHOR]