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Objective: To explore the chemical constituents for the seeds of Cicer arietinum Linn., and lay the foundation for clarifying the nutritional function and medicinal value of the title plants from the perspective of pharmacodynamic material basis. Methods: The 75% ethanol extract of C. arietinum was dispersed in water, and extracted extensively by petroleum ether, ethyl acetate, and n-butanol, respectively, to obtain different extraction. The n-butanol extract was isolated and purified by normal phase silica gel, Sephadex LH-20, and semi-preparative high performance liquid chromatography. The structures of the purified compounds were elucidated by 1 H and 13C-NMR spectroscopy. Results: Eight compounds were identified from the seeds of C. arietinum. Their structures were identified as (+)pinitol (1), ethyl-α-D-galactopyranoside (2), L-tryptophan (3), β-sitosterol (4), β-daucosterol (5), methyl linoleate (6), linoleic acid (7), and quercetin 3-O-β -D-glucopyranoside (8). Conclusion: Methyl linoleate (6) and quercetin 3-O-β -Dglucopyranoside (8) were identified firstly from the seeds of C. arietinum. The structure of previous reported ethyl α-L-galactopyranoside in the seeds of C. arietinum was confirmed as ethyl α-D-galactopyranoside (2). [ABSTRACT FROM AUTHOR]

Objective: To study the chemical constituents of lignans and flavonoids from the Mongolian medicinal material Clematis aethusifolia Turcz., and to explore the basis of its active substances. Methods: The whole plants of C. aethusifolia was extracted by 75% ethanol. The extract was concentrated in vacuo to remove alcohol and suspended in water. And then, it was partitioned with petroleum ether, ethyl acetate, and n-butanol, respectively. The n-butanol extract was isolated and purified by various column chromatography techniques. The structures of the purified compounds were identified by MS (Mass spectrometry), 1H and 13C NMR (Nuclear magnetic resonance) spectroscopy. Results: Three lignans and three flavonoids were identified from C. aethusifolia. Their chemical structures were elucidated as (+) -syringaresinol-4'-O-茁-D-glucopyranoside (1), (+)-pinoresionol-4'-O-茁-D-glucopyranoside (2), (7R,8S,7'R,8'S,7'S,8'R)-4'-guaiacylglyceryl-medioresinol-4-O-茁-D-glucopyranoside (3), quercetin (4), quercetin 3-O-茁-D-rutinoside (5), and quercetin-7-O-茁-D-glucopyranoside (6), respectively. Conclusion: Compounds 3 and 6 were identified for the first time from C. aethusifolia, and compound 3 was discovered firstly from the genus of Clematis. [ABSTRACT FROM AUTHOR]