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The article studies the experience of Jesuit missionaries who worked in the "Austral Missions" (1750-1753) in the current territory of the Province of Buenos Aires. The sources that support the research are letters and reports from Jesuits and members of the colonial government, referring to the foundation, functioning and abandonment of that "pueblos de indios". It will be critically analyzed seeking to understand the effects of this experience on the subjectivity of religious people. Therefore, in addition to working with sources, we will also make use of comparisons with situations of a similar nature, in other mission areas, as well as the debate with historiography. [ABSTRACT FROM AUTHOR]