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Urban comprehensive carrying capacity is an important guarantee and external representation of regional sustainable development. Based on urban comprehensive carrying capacity, this paper constructed a performance evaluation index system of urban comprehensive carrying capacity, and used entropy method, urban comprehensive carrying capacity measurement model and urban sustainable development model to measure spatial and temporal comprehensive carrying capacity of Harbin City in Heilongjiang Province from 2012 to 2017. The results show that: 1) index weight analysis suggested the regional development mode in Harbin still followed an epitaxial development mode, which pursued the expansion of scale and the growth of total amount of regional development, neglecting the effective utilization of resources and the improvement of structural benefits. 2) In the pressure system, the index of resource support has dropped sharply. The index of environmental capacity and social progress has risen circuitously, while the degree of agglomeration and the value of transportation facilities have risen steadily; in the pressure system, the index of population development and economic growth tended to fluctuate, while energy consumption and environmental pollution showed a more synchronous change in characteristics, and the livable demand remained at a high level. 3) The carrying capacity index of resources and environment in Harbin has been declining, and the acquired carrying capacity index fluctuated. The long-term regional development model has severely impaired the carrying capacity of resources and environment. 4) The comprehensive carrying capacity of Harbin has clear spatial differentiation characteristics. Finally, the paper proposes that location conditions, economic development level, government regulation, and science and technology are the main factors influencing the spatial differentiation of urban comprehensive carrying capacity. [ABSTRACT FROM AUTHOR]