1. GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage1[w][OPEN].
- Author
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Kong, Yingzhen, Gongke Zhou, Abdeen, Ashraf A., Schafhauser, James, Richardson, Beth, Ahnodjo, Melani A., Jung, Jiyoung, Wicker, Louise, Mohnen, Debra, Western, Tamara, and Hahn, Michael G.
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ARABIDOPSIS , *MUCILAGE , *IMMUNOLOGY , *ENDOPLASMIC reticulum , *GENES - Abstract
The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; Atlg02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATLS is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATLS is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATLS gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgat15-1 mutants with the wild-type AtGATLS gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgat15-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgat15-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATLS might function in the regulation of the final size of the mucilage rhamnogalacturonan I. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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