1. Evaluation of optimum conditions for Achyranthes bidentata polysaccharides encapsulated in cubosomes and immunological activity in vitro.
- Author
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Ou, Ning, Sun, Yaqing, Zhou, Shuzhen, Gu, Pengfei, Liu, Zhengguang, Bo, Ruonan, Hu, Yuanlaing, Liu, Jiaguo, and Wang, Deyun
- Subjects
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POLYSACCHARIDES , *AMARANTHACEAE , *DRUG delivery systems , *CHINESE medicine , *IMMUNOLOGICAL adjuvants - Abstract
Cubosomes, as biocompatible carriers in drug delivery systems, consist of curved bicontinuous lipid bilayers. With a honeycombed structure divided into two internal aqueous channels, cubosomes could be used for many bioactive ingredients. Achyranthes bidentata polysaccharides (ABPs) are isolated from the roots of Achyranthes bidentata , used in Chinese herbal medicine, and present a noticeable effect as an immunomodulator. This study investigates the optimal preparation of combined cubosome-ABP (Cub-ABP) nanoparticles using response surface methodology and explores their characteristics and stability. The encapsulation efficiency of optimized Cub-ABPs was 72.59%. In-vitro stability studies demonstrated the stability of Cub-ABPs and cubosome nanoparticles without ABPs; both were stable for up to 25 days. Safe concentrations of Cub-ABPs and cubosome nanoparticles without ABPs are 104.06 μg/mL and 208.13 μg/mL with comparatively low cytotoxicity against lymphocytes. Moreover, the feasible immunomodulatory effects of Cub-ABPs were determined by evaluating their proliferation and change of CD4 + /CD8 + ratio on splenic lymphocytes in vitro . Proliferation and flow cytometry studies revealed that, compared with free ABPs and blank cubosomes, Cub-ABPs proved more effective in promoting lymphocyte proliferation and in triggering the transformation of T-lymphocytes into T h -cells. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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