The late Paleozoic glacial-to-postglacial turnover evolved complexly across Gondwana. Successions bearing volcaniclastic material that can be radiometrically dated provide crucial information about the timing of those climate events. The southernmost part of the Paraná Basin, for instance, has a high-precision geochronological framework. The eastern sector of this basin (Paraná State and north of Santa Catarina State), however, lacks radiometric ages, but conversely, has a more complete stratigraphic record, and paleontological information still poorly explored for the purpose of biostratigraphic correlation. This work examines the glacial-to-postglacial interval in the Paraná State, represented by the upper Itararé Group (Taciba Formation; glacial) and lower–middle Rio Bonito Formation (postglacial). Sedimentological, paleontological and geochemical data from outcrops, cores and well logs were used to decipher the timing, paleoclimatic and paleogeographic scenarios of this transition. The examined succession comprises four stacked units (U1 to U4, from older to younger). Diamictite-dominated units (U1 and U3), here interpreted as consecutive glaciation–deglaciation events, are separated by non-glacial, continental to shallow marine deposits, commonly bearing fossil plants and coal seams (U2). An important transgression followed the first deglaciation, which is equivalent to the " Eurydesma transgression", based on the presence of marine invertebrates of the homonymous fauna in the Passinho Shale. U2 holds elements of two different floras, i.e., Phyllotheca – Gangamopteris (P–G), predating the " Eurydesma transgression", and Glossopteris – Brasilodendron (G–B), above the transgression. Therefore, U2 is interpreted as an interglacial interval, once it records a climate improving before the last glacial episode of U3, which is further supported by relatively high values of the Chemical Index of Alteration (CIA). Deposits of U3, associated with a decrease in the value of the CIA, are unconformably overlain by U4. The occurrence of coal-bearing postglacial facies (U4) associated with the G–B Flora, coincides with an increase in the CIA values. Sediment transport was toward the SW in all units and in the same direction it is observed an overall thinning of U1 and U3 and thickening of U2. The interglacial P–G Flora of the study area correlates with postglacial southernmost floras, based on U–Pb CA-TIMS Asselian ages of tonsteins. Correlation of the Eurydesma fauna-containing Passinho Shale with equivalent successions with high-precision age control in southern Africa, allowed us to position both deglaciations of the Taciba Formation (U1 and U3) in the Asselian. These findings suggest that U1 and U3 record two early Permian glacial episodes, with the younger one (U3) disappearing southward. Accordingly, our results indicate that the glacial-to-postglacial turnover was diachronous along the eastern belt of the Paraná Basin, being progressively older southward, considering that interglacial fossil plant assemblages in the eastern margin correspond in time to postglacial assemblages farther south. • The examined succession includes two glaciation-deglaciation stages insterspersed by interglacial deposits and an uppermost postglacial interval. • The interglacial stage is time equivalent to postglacial deposits farther south, suggesting that the last glaciation was limited to NE Paraná Basin • Biostratigraphy based on floral and faunal assemblages indicates an early Permian age. • The glacial–postglacial transition is diachronous along the eastern margin of the basin. [ABSTRACT FROM AUTHOR]