Search

In recent years, developing rapid, simple, and effective new methods to monitor food safety is standing out enough to be noticed. Electrospinning is an easy, versatile, and low-cost technique to fabricate multifunctional micro-and nanomaterials. The combination of electrospinning and biosensors is one of the most promising strategies and is expected to replace traditional detection materials. This paper reviews the research progress of electrospinning in food detection, including electrospinning materials generally used in the food field with their benefits of high aspect ratio, large specific surface area, tunable porosity, easy functionalization, easy handling, mechanical stability, such as natural polymers, synthetic polymers or the mixture of both. Besides, we also emphatically introduced the application of their intrinsic characteristics combined with biosensing platforms and list the sensitivity and detection limit of various methods and materials in detection, as well as their application potential, and the challenges faced by the on-site detection and the large-scale commercial applications in the food industry as future exploration headings, giving new thinking of nanomaterial applications for food safety detection. [ABSTRACT FROM AUTHOR]

A novel, integrated, real‐time electrochemical loop‐mediated isothermal amplification (LAMP) device for pathogen detection in food was developed. The electrochemical LAMP device for detection of the vt gene in E. coli O157 : H7 and invA gene in Salmonella enterica was implemented with DNA intercalating probes‐methylene blue. To improve assay efficiency, the optimal concentration of MB was determined to be 30 μM. The real‐time electrochemical LAMP results of gene serial dilutions were compared to the fluorescence‐based detection method. For E. coli O157 and Salmonella, the assay detection limits were 10 DNA copies and 1 DNA copy, respectively, in a reaction time of less than 30 min. The electrochemical and optical LAMP method analyses were realized with 10 % milk to simulate the turbid environment of real food samples. In real‐time LAMP of Salmonella in a turbid environment, the detection limit of 10 copies without pretreatment was possible using the electrochemical method only. Our electrochemical device was used for bacterial detection in 150 turbid food samples, including juice, milk, and soy milk. This electrochemical device has many advantages, including size, portability, high specificity, and high sensitivity, while also being a user‐friendly device that provides stable signals. Thus, to the best of our knowledge, this new electrochemical device demonstrates the best performance for bacterial detection in the rapid contaminated food detection category. [ABSTRACT FROM AUTHOR]