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Activated carbon (AC) supported palladium cobalt bimetallic nanoparticles (PdCo@AC NPs) were obtained by green synthesis method using Cinnamomum verum (C. Verum) extract. The obtained NPs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Crystallography (XRD), Transmission Electron Microscope (TEM) and Ultraviolet Visible (UV-VIS) spectroscopy, and the functional groups and morphology of the nanoparticle were elucidated. The resulting particle size was found to be 2.467 nm. NPs were evaluated using Cyclic Voltammetry (CV), Scan Rate (SR), and Differential Pulse Voltammetry (DPV) techniques for potential dopamine sensors application. According to the obtained DPV results, Limit of Detection (LOD) and Limit of Quantitation (LOQ) values are found to be 5.68 pM and 17.21 pM, respectively. It was also observed that AC supported PdCo nanoparticles obtained from C. verum extract sensed dopamine quite well. Besides, to examine the antibacterial properties of NPs, antibacterial analyzes were performed with Escherichia coli (E. Coli) and Staphylococcus aureus (S. Aureus). It was observed that it showed good antibacterial properties against gram positive (S. aureus) and gram negative (E. coli) bacteria. The study gave important results in terms of the synthesis of bimetallic NPs using the green synthesis method and their usability in different areas. With this study, it was observed that a good antibacterial dopamine sensor were obtained with the successful biogenic synthesis of AC supported PdCo bimetallic NPs. [ABSTRACT FROM AUTHOR]