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The dual quaternion plays an advantageous role in the field of robotic kinematics and dynamics due to its compact expression. It is well known that quaternion can be easily implemented as algebraic operations on vectors through matrix operators. Similarly, the dual matrix operators can convert dual quaternion operations to matrix operations. However, it has not received adequate attention, and thus there are very few applications at present. With the dual matrix operators of dual quaternion, this paper re-verifies the equivalency between the conjugate formula of unit dual quaternion and dual Euler–Rodrigues formula. To combine this equivalence with the homomorphic mapping of Lie groups, a theoretical correlation of the current hand-eye calibration methods is established. Motivated by the attempt to establish a more complete scheme for hand-eye calibration, a new simultaneous method based on the conjugate formula of dual quaternion is proposed. The method verifies that the rotation problem can be extended to the rigid-body transformation problem via the dual algebra. Finally, the simulation and experimental validations for the hand–eye calibration method are given. • The conjugate formula of unit dual quaternion is re-verified via the dual operations. • A new simultaneous method is defined for hand-eye calibration. • The theoretical correlation with the existing methods is established. [ABSTRACT FROM AUTHOR]