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Objective To investigate the effects of metformin on the proliferation, apoptosis and migration ability of human hepatocellular carcinoma cells HCCLM3. Methods HCCLM3 cells were treated with different concentrations of metformin. Cell proliferation was assessed by CCK-8 assay. The expression of related apoptosis genes, Bax and Bcl-2 were determined by RT-PCR. Cell migration was determined by scratch experiment. Results Metformin decreased the proliferation of HCCLM3 cells in a dose-dependent and time-dependent manner and the cell proliferation inhibition rate was significantly higher than the control group (P < 0.05) . The migration of HCCLM3 was inhibited when the concentration of metformin reaches 10mmol/L. The expression of Bax mRNA was up-regulated, while Bcl-2 was down-regulated after 48h treated. Conclusion Metformin can inhibit HCCLM3 cell proliferation and migration, and affect the expression of apoptosis related gene Bax and Bcl-2 in a concentration and time-dependent manner. [ABSTRACT FROM AUTHOR]

Currently, the top k query algorithms about the uncertain XML data can not deal with continuous uncertain data. The SPCProTJFast algorithm is proposed, which improves the traditional merging algorithm, combines with continuous uncertain data filtering methods, implements the top-k query algorithm over continuous uncertain XML data. In order to avoid the impact of too small probability limit on filtering effect, the HPCProTJFast algorithm is proposed, which delays the handling of continuous types of nodes and visits the continuous nodes only when the entire twig that meets the probability condition are acquired. Experimental results show that, in terms of the execution time and the filtration efficiency, these two algorithms are more efficient than the ProTJFast algorithm that deals with continuous uncertain data directly, and the HPCProTJFast algorithm is the most efficient. [ABSTRACT FROM AUTHOR]