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• Efficient extracellular proton transfer (EPT) was achieved in anaerobic digestion. • Efficient EPT promoted the participation of water in methane generation. • Efficient EPT facilitated the proton motive force generation and ATP synthesis. • A new principle of EPT propelling energy-conserving methanogenesis was proposed. Anaerobic digestion (AD) is a promising technology to realize the conversion from organic matters to methane, which is highly mediated by syntrophic microbial community via mutualistic interactions. However, small energy available in methanogenic conversion usually limits the metabolic activity. To adapt such energy-limited environment, efficient energy conservation is critical to support active physiological functions of anaerobic consortia for methanogenic metabolism. In this study, the contribution of extracellular proton transfer (EPT) enhancement to achieving energy-conserving methanogenesis in AD was explored. Proton-conductive medium (PCM) was applied to construct efficient proton transport pathway, and a large number of protons from extracellular water were found available to upregulate methanogenesis in AD, as indicated by the increase in the content of 2H (D) in methane molecules (over 40.7%), among which CO 2 -reduction-to-CH 4 was effectively enhanced. The increases of adenosine triphosphate (ATP) concentration (+54.1%) and gene expression activities related to ATPase (+100.0%) and proton pump (+580.1%) revealed that enhanced EPT by PCM promoted transmembrane proton motive force generation to facilitate ATP synthesis. Based on genome-centric metatranscriptomic analyses, MAG14, MAG63 and MAG61 with high energy conservation activity displayed most pronounced positive response to the EPT enhancement. In these core MAGs, the metabolic pathway reconstruction and the key genes activity identification further proved that EPT enhancement-driven efficient ATP synthesis stimulated the cross-feeding of carbon and proton/electron to facilitate microbial mutualism, thereby resulting in the high energy-conserving methanogenesis. Overall, our work provides new insights into how EPT enhancement drives high energy-conserving methanogenesis, expanding our understanding of the ecological role of EPT in AD. [Display omitted] [ABSTRACT FROM AUTHOR]

• Magnetic biochar addition caused a loosed structure of protein for boosting CH 4 yield. • Fe 2 O 3 and FeO of magnetic biochar first bound with proteins via hydrogen bond. • The C=O, C N and N H of protein were subsequently destroyed by magnetic biochar. • Hydrolysis-acidification and syntrophic metabolisms were promoted by magnetic biochar. The addition of composite conductive materials is being increasingly recognized as a promising strategy to enhance anaerobic digestion (AD) performance. However, the influence of these materials on protein hydrolysis has been poorly documented. Here, a novel magnetic biochar derived from oil sludge and straw was synthesized using different iron sources and successfully applied in sludge AD. Experimental results revealed that magnetic biochar modified by Fe2+ exhibited excellent electron transfer capacity, moderate magnetization, diverse functional groups (e.g. C=O, C-O=O-), and abundant iron distribution. These characteristics significantly enhanced the hydrolysis of tryptophan-like components, leading to increased methane production (144.44 mL gVS−1 vs 79.72 mL gVS−1 in the control test). Molecular docking analysis revealed that the binding of magnetic biochar related Fe2+ and Fe3+, onto sludge proteins via hydrogen bond played a key role in promoting subsequent protein hydrolysis. Additionally, the noteworthy conservation of protein structures from α-helix and β-sheet to random coil, along with the breakdown of the amide I-associated C=O group and amide III-related C N and N H bonds following the addition of magnetic biochar, accelerated the degradation of sludge protein. Observation of variations in protease activity, coenzyme F420, electron transfer system (ETS), and conductivity within the AD systems, particularly the enrichment of Methanospirillum and Methanosaeta archaea , as well as the Petrimonas, Comamonas, and Syntrophomonas bacteria, suggested that magnetic biochar facilitated a conducive environment by improving hydrolysis-acidification and the direct interspecies electron transfer (DIET) process for acetoclastic methanogens. Moreover, metabolic pathways further proved that tryptophan metobalism and acetoclastic methanogenesis were both facilitated by magnetic biochar. This study provides an in-depth understanding of the impact of magnetic biochar on protein hydrolysis in sewage sludge AD. [Display omitted] [ABSTRACT FROM AUTHOR]

• Electrochemical pretreatment (EPT) inactivated methanogenesis without chemicals. • EPT–AD achieved carbon fixation 2.5 times via VFA accumulation (2563±308 mg COD/L). • Interfacial thermodynamics analysis revealed sludge/microbes' aggregation tendency. • Protein–protein interactions between MMCE, S–layer, HDR and alaS were investigated. Capturing the carbon in volatile fatty acids (VFA) produced from the anaerobic digestion (AD) of sewage sludge has the potential to not only provide economic benefits but also reduce greenhouse gas production. This study demonstrates a chemical–free method to collect VFA from an AD instead of methane that involves electrochemical pretreatment (EPT) of sludge. Experimental results show that applying 15 V EPT for 45 min enhances acidogenesis and selectively inhibits methanogenesis, leading to a substantial VFA accumulation (2563.1 ± 307.9 mg COD/L) and achieving 2.5 times more carbon fixation than via methane production. Interfacial thermodynamic analysis shows that EPT induces a decrease in both the repulsive electrostatic energy (from 152.9 kT to 12.2 kT) and the energy barrier (from 57.0 kT to 2.6 kT) in the sludge, leading to increased sludge aggregation and entrapment of microorganisms. Molecular docking sheds lights on how the methanogens interacts with the organic matter released from EPT (e.g., alanine–tRNA ligase), showing that these interactions potentially interfere with the proteins that are associated with the activities of the methanogens and the electron transfer pathways, thereby impeding methanogenesis. Integrating EPT into AD therefore facilitates the recovery of valuable VFA and the capture of carbon from freshwater sludge, providing notable economic and environmental benefits in sewage sludge treatment. [Display omitted] [ABSTRACT FROM AUTHOR]

• 45 °C for 48 h and 55 °C for extra 48 h was the optimal sludge pre-treatment method. • Low-temperature thermal hydrolysis was improved with increasing solids content. • The optimal pre-treatment enhanced methane yield by 66.39 %. • Pre-treatment and sludge thickening led to increased abundance of specific microbes. • Foaming was observed at an organic loading rate of 2.00 g VS/(L Reactor ·day). This study investigated the impact of two low-temperature thermal pre-treatments on continuous anaerobic reactors' performance, sequentially fed with sludge of different total solids content (∼3 % and ∼6 %) and subjected to progressively increasing Organic Loading Rates (OLR) from 1.0 to 2.5 g volatile solids/(L Reactor ⋅day). Assessing pre-treatments' influence on influent sludge characteristics revealed enhanced organic matter hydrolysis, facilitating sludge solubilization and methanogenesis; volatile fatty acids concentration also increased, particularly in pre-treated sludge of ∼6 % total solids, indicating improved heating efficiency under increased solids content. The reactor fed with sludge pre-treated at 45 °C for 48 h and 55 °C for an extra 48 h exhibited the highest methane yield under all applied OLRs, peaking at 240 ± 3.0 mL/g volatile solids at the OLR of 2.5 g volatile solids/(L Reactor ⋅day). 16S rRNA gene sequencing demonstrated differences in the reactors' microbiomes as evidence of sludge thickening and the different pre-treatments applied, which promoted the release of organic matter in diverse concentrations and compositions. Finally, the microbial analysis revealed that specific foam-related genera increased in abundance in the foam layer of reactors' effluent bottles, dictating their association with the sludge foaming incidents that occurred inside the reactors during their operation at 2.0 g volatile solids/(L Reactor ⋅day). [Display omitted] [ABSTRACT FROM AUTHOR]

• Dead sludge biomass skewed DNA-based functional predictions for anaerobic digestion. • PMA-seq is a promising approach for assessing the active biomass of anaerobic sludge. • Potential predictive value of dead-biomass DNA for process-relevant metabolites. • Two universal primer candidates for amplicon sequencing of anaerobic sludge. DNA-based monitoring of microbial communities that are responsible for the performance of anaerobic digestion of sewage wastes has the potential to improve resource recoveries for wastewater treatment facilities. By treating sludge with propidium monoazide (PMA) prior to amplicon sequencing, this study explored how the presence of DNA from dead microbial biomass carried over with feed sludge may mislead process-relevant biomarkers, and whether primer choice impacts such assessments. Four common primers were selected for amplicon preparation, also to determine if universal primers have sufficient taxonomic or functional coverage for monitoring ecological performance; or whether two domain-specific primers for Bacteria and Archaea are necessary. Anaerobic sludges of three municipal continuously stirred-tank reactors in Victoria, Australia, were sampled at one time-point. A total of 240 amplicon libraries were sequenced on a Miseq using two universal and two domain-specific primer pairs. Untargeted metabolomics was chosen to complement biological interpretation of amplicon gene-based functional predictions. Diversity, taxonomy, phylogeny and functional potentials were systematically assessed using PICRUSt2, which can predict community wide pathway abundance. The two chosen universal primers provided similar diversity profiles of abundant Bacteria and Archaea, compared to the domain-specific primers. About 16 % of all detected prokaryotic genera covering 30 % of total abundances and 6 % of PICRUSt2-estimated pathway abundances were affected by PMA. This showed that dead biomass in the anaerobic digesters impacted DNA-based assessments, with implications for predicting active processes, such as methanogenesis, denitrification or the identification of organisms associated with biological foams. Hence, instead of running two sequencing runs with two different domain-specific primers, we propose conducting PMA-seq with universal primer pairs for routine performance monitoring. However, dead sludge biomass may have some predictive value. In principal component analysis the compositional variation of 239 sludge metabolites resembled that of 'dead-plus-alive' biomass, suggesting that dead organisms contributed to the potentially process-relevant sludge metabolome. [Display omitted] [ABSTRACT FROM AUTHOR]

• HTL aqueous from mixed sludge cake at ≤ 340 °C was readily biodegradable aerobically. • HTL aqueous was 50 % and 32 % biodegradable under mesophilic and thermophilic AD. • HTL aqueous obtained at 360 °C was highly inhibitory for mesophilic/thermophilic AD. • HTL aqueous from mixed sludge cake was found unsuitable for dark fermentation. • HTL aqueous aerobic treatment and mesophilic AD were identified as feasible options. Aerobic treatment, mesophilic anaerobic digestion, thermophilic anaerobic digestion, and dark fermentation were evaluated for on-site biological treatment of municipal sludge derived HTL aqueous. For all four described batch test scenarios, municipal sludge-derived HTL aqueous samples obtained under 290–360 °C and 0–30 min retention time were used. In the aerobic respirometric tests, HTL aqueous samples resulted in a five-day biochemical oxygen demand range of 40.75 g/L (350 °C-25.6 min) to 54 g/L (325 °C-0 min). The calculated aerobic biodegradability index showed that approximately 50 % of the organics in HTL aqueous were easily biodegradable. Mesophilic and thermophilic biochemical methane potential tests resulted in specific yields of 151–179 mL CH 4 /g chemical oxygen demand (COD) and 103–122 mL CH 4 /g COD, respectively. HTL aqueous obtained under 360 °C-15 min condition caused total inhibition in both mesophilic and thermophilic anaerobic digestion. Possible causes for this inhibition were pyridine, pyrrolidinone, piperidinone, pyridinol, and phenolic compounds, which were higher in abundance in the 360 °C-15 min sample. HTL aqueous was found unfit for hydrogen production in dark fermentation due to inhibitory composition. In summary, on-site biological treatment of HTL aqueous was found to be most suitable under aerobic and mesophilic anaerobic conditions. [Display omitted] [ABSTRACT FROM AUTHOR]

• Optimal deployment of thermal hydrolysis and anaerobic digestion was studied. • The proposed characteristics-based AD–TH–AD achieved maximum net energy output. • Maximum net energy output increased by 48.6% compared with traditional TH–AD. • Refractory compounds formed in TH were firstly evaluated by simulated A2O process. • Characteristics-based AD–TH–AD reduced refractory compounds by over 15.2%. Thermal hydrolysis (TH) is widely employed in combination with anaerobic digestion (AD) to efficiently treat primary sludge and waste-activated sludge in municipal wastewater treatment plants. In this study, four different scenarios—conventional AD (S1), TH–AD (S2), AD–TH–AD (S3), and characteristics-based AD–TH–AD (S4, primary AD only for primary sludge)—were evaluated to determine the optimal deployment of TH and AD for treating primary sludge and waste-activated sludge to maximize net energy output. The maximum net energy output of 4899 MJ/t-TS fed (per ton total solids of sludge fed) was achieved in S4 when assuming the recovered heat was only used for AD heating and surplus heat was wasted, and the net energy output of S4 was 70.8 % higher than that of S1 and 48.6 % higher than that of S2. This remarkable improvement was attributed to a reduction of > 15.2 % in refractory compounds, resulting in a 17 % increase in methane yield. Importantly, this study provides the first comparison of refractory compounds between inter-thermal hydrolysis (inter-TH) and pre-thermal hydrolysis (pre-TH) using a simulated A2O process. Overall, this study provides innovative insights and strategies for enhancing the TH and AD process performance based on the specific characteristics of sewage sludge derived from wastewater treatment plants. [Display omitted] [ABSTRACT FROM AUTHOR]

Coupling anaerobic digestion and algae cultivation has attracted attention as a sustainable means of treating high-strength wastewaters. In such a scenario, nutrients from the liquid anaerobic digestate are used by algae to produce biomass. However, use of full-strength digestate results in poor algal growth and nutrient removal. Most researchers have overcome this challenge by diluting digestate 10–30 fold prior to algae growth but such dilution rates demand large amounts of fresh water, posing challenges for scale-up. The objectives of this study were to 1) assess whether ammonium, turbidity, and heavy metals in digestate were the primary sources of inhibition for a highly-nutrient tolerant strain of Chlorella sorokiniana, and, 2) develop a biological pretreatment strategy to overcome algal growth inhibition on full strength digestate. Ammonia toxicity, turbidity, and heavy metals have been commonly hypothesized as the source of algal growth inhibition, but our results showed that these factors were not critical inhibitors of C. sorokiniana. Dose response studies showed that C. sorokiniana could grow robustly on 3,500 mg/L ammonium. Regardless, full strength digestates of wastewater sludge and food waste were very inhibitory to C. sorokiniana. We utilized a pretreatment approach using activated sludge which led to robust algal growth on full-strength digestate. High growth rates of 250–500 mg/L/d were achievable on pretreated digestates despite very high ammonium levels of ∼2,000 mg/L. Pretreating digestate also led to significantly faster algal nutrient uptake compared to untreated digestate (p < 0.001). Image 1 • Algal growth was inhibited on full-strength anaerobic digestates. • Growth inhibition was not due to ammonia, turbidity, or heavy metals. • sorokiniana grew rapidly on ammonium concentrations up to 3.5 g/L. • Aerobic bacteria pretreatment removed inhibitors yielding robust algal growth. • Ypothesize that organic compounds in digestate are inhibitory to algae. [ABSTRACT FROM AUTHOR]

Abstract Mesophilic batch anaerobic digesters fed by different substrates were set up to identify the role of substrate rheology in anaerobic digestion performance while operating below the toxic level. Five substrates of different rheological behaviour but at the same amount of organic matters were prepared by addition of different amount of an inert material (0, 0.03, 0.07, 0.11, and 0.20 g) per g of waste activated sludge (WAS). To gain a comprehensive insight, the interactive relationship between substrate rheology, physico-chemical properties and biogas production as well as digestate dewaterability was investigated. The results proved that better access of microorganisms to organic matters improved the digester performance and led to 19.29% and 12.5% increase in biogas yield and VS removal efficiency, respectively. Moreover, the statistical analysis showed that consistency index and loss modulus of sludge could be employed as promising indications for biogas yield while yield stress could predict dewaterability of digestate as far as the other physico-chemical properties remained unchanged. During digestion measurement of consistency index and loss modulus of digestate could be performed as a reliable tool to monitor biogas production. Graphical abstract Image 1 Highlights • Improvement of anaerobic digester performance by increasing substrate fluidity. • Biogas production increased linearly by reduction of consistency index of substrate. • Biogas production increased linearly by reduction of loss modulus of substrate. • Dewaterability improved linearly by reduction of yield stress of sludge. • Rheological measurements can be used as a tool to monitor anaerobic digestion process. [ABSTRACT FROM AUTHOR]

Humification plays an important role in the sewage sludge stabilization treatment and probably influences the end-product ecotoxicity, which are not fully understood for high-solid anaerobic digestion (AD) process. This study monitored the aromaticity degree of humic acid-like fraction (HA) and detected digestate phytotoxicity during a 48-d high-solid AD. Significant repolymerization of aromatic structures in HA. Electrical conductivity (EC) of digestate changed negatively with the aromaticity degree in HA, and the digestate with high EC had low seed germination index (SGI). A positive correlation between SGI and the aromaticity degree of HA was found. Aromatic repolymerization of HA supplies more aromatic electron-rich sites to form neo-humic polymers and to determine the distribution of organic and/or inorganic phytotoxic compounds; thus, aromatic repolymerization of HA may control the digestate phytotoxicity by reducing excessive salinity. The results showed the effect of HA repolymerization on digestate stabilization and phytotoxicity reduction during the high-solid AD process. The inadequate phytotoxicity reduction efficiency (SGI ≤ 17%) may be ascribed to unaccomplished HA repolymerization, creating a demand for humification intensification studies. [ABSTRACT FROM AUTHOR]

Post-anaerobic digestion (PAD) treatment technologies have been suggested for anaerobic digestion (AD) to improve process efficiency and assure hygenization of organic waste. Because AD reduces the amount of organic waste, PAD can be applied to a much smaller volume of waste compared to pre-digestion treatment, thereby improving efficiency. In this study, dewatered digestate cakes from two different AD plants were thermally hydrolyzed and dewatered, and the liquid fraction was recirculated to a semi-continuous AD reactor. The thermal hydrolysis was more efficient in relation to methane yields and extent of dewaterability for the cake from a plant treating waste activated sludge, than the cake from a plant treating source separated food waste (SSFW). Temperatures above 165 °C yielded the best results. Post-treatment improved volumetric methane yields by 7% and the COD-reduction increased from 68% to 74% in a mesophilic (37 °C) semi-continuous system despite lowering the solid retention time (from 17 to 14 days) compared to a conventional system with pre-treatment of feed substrates at 70 °C. Results from thermogravimetric analysis showed an expected increase in maximum TS content of dewatered digestate cake from 34% up to 46% for the SSFW digestate cake, and from 17% up to 43% in the sludge digestate cake, after the PAD thermal hydrolysis process (PAD-THP). The increased dewatering alone accounts for a reduction in wet mass of cake leaving the plant of 60% in the case of sludge digestate cake. Additionaly, the increased VS-reduction will contribute to further reduce the mass of wet cake. [ABSTRACT FROM AUTHOR]

In this study, the occurrence and fate of 49 pharmaceuticals and personal care products (PPCPs) were investigated in an anaerobic-anoxic-oxic (A2/O) wastewater treatment plant (WWTP) for seven consecutive days using 24-h composite sampling technique. Special emphasis was placed to understand the distribution of PPCPs in dissolved and adsorbed phase, and to evaluate PPCP fate in different treatment units. Among the 49 PPCPs, 40 PPCPs in influent, 36 in effluent, 29 in sludge and 23 in suspended solids were detected at least once during sampling. Non-steroidal anti-inflammatory drugs (NSAIDs) and a stimulant were predominant PPCPs in influent whereas antibiotics were predominant in sludge, effluent and suspended solids. Removal efficiencies from the aqueous phase based on the dissolved PPCPs showed variable contribution in removing different PPCPs under screen and grit chamber, anaerobic treatment, anoxic treatment, oxic treatment and sedimentation-UV treatments, with the highest removal percentage by anaerobic process in terms of both individual and overall treatment. Mass load analysis showed that 352 g PPCPs enter the WWTP daily while 14.5 g and 58.1 g were discharged through effluent and excess sludge to the receiving sea water and soil applications, respectively. Mass balance analysis based on both aqueous and suspended PPCPs showed 280 g (79.4%) mass of influent PPCPs was lost along the wastewater treatment processes, mainly due to degradation/transformation. [ABSTRACT FROM AUTHOR]

Microwave (MW) sludge pretreatment systems are usually limited to a frequency of 2.45 GHz and the heating frequency is constrained by commercially available hardware. Studies using MW heating at this frequency have reported negative net energy balance (output energy as methane minus input electrical energy). This necessitates further research into more efficient thermal pretreatment technologies. In this research, a novel and highly efficient radio frequency (RF) pretreatment system at a frequency of 13.56 MHz was designed, implemented, and tested for the first time. The system was custom-designed based on the dielectric characteristics of thickened waste activated sludge (TWAS) to achieve a very efficient and uniform heating system. The effects of three factors including pretreatment method (RF vs. MW), final temperature (60, 90 and 120 °C), and stationary (holding) time (0, 1 and 2 h) on sludge solubilization and performance of mesophilic batch anaerobic digestion were evaluated simultaneously. Energy measurements were also made to compare the efficiency of the custom-designed RF and conventional MW heating systems. The differences in sludge disintegration (solubilization) using the RF and MW pretreatment systems were negligible (P > 0.05). No statistically significant difference was also observed between the two pretreatment systems in terms of mesophilic biogas production rate and extent (P > 0.05). The energy efficiency of the RF pretreatment system was measured between 67.3 and 95.5% for the temperature range of 25–120 °C which was significantly higher than that of the MW system efficiency which varied from 37 to 43%. Overall, the average input energy of the RF system was less than half of the energy consumed during the operation of the MW system to achieve a same target temperature. Considering the results of this research, the RF heating at a frequency of 13.56 MHz is suggested as an effective and energy-efficient technique for thermal hydrolysis of TWAS. [ABSTRACT FROM AUTHOR]

To test the feasibility and practicability of the process combing hydrothermal pretreatment for dewatering with biogas production for full utilization of sewage sludge, hydrothermal/alkaline hydrothermal pretreatments and in turn anaerobic digestion of the filtrates obtained after dewatering the pretreated sludge were performed at bench- and pilot-scales. The hydrothermal temperature fell within the range of 140 °C–220 °C and the pretreatment time varied from 30 min to 120 min. For the alkaline hydrothermal pretreatment the pH value of the sludge was adjusted to 9.0–11.0 by adding Ca(OH) 2 . The results showed that the dewaterability of the sewage sludge was improved with increasing pretreatment temperature but the impact of the pretreatment time was not significant. The addition of Ca(OH) 2 gave better performance on the subsequent mechanical dewatering of the pretreated sludge compared to pure hydrothermal pretreatment, and the higher the pH value was, the better the dewaterability of the pretreated sludge was. The conditions of 180 °C/30 min and 160 °C/60 min/pH = 10.0 (for hydrothermal and alkaline hydrothermal pretreatments, respectively) resulted in relatively good results in the theoretical energy balance, which were verified in the pilot-scale tests. Based on the data from the pilot tests, the alkaline hydrothermal process realized self-sufficiency in energy at the cost of a proper amount of CaO. [ABSTRACT FROM AUTHOR]

The present study examines the relationship between the degree of solubilization and biodegradability of wastewater sludge in anaerobic digestion as a result of low-temperature thermal pre-treatment. The main effect of thermal pre-treatment is the disintegration of cell membranes and thus solubilization of organic compounds. There is an established correlation between chemical oxygen demand (COD) solubilization and temperature of thermal pre-treatment, but results of thermal pre-treatment in terms of biodegradability are not well understood. Aiming to determine the impact of low temperature treatments on biogas production, the thermal pre-treatment process was first optimized based on an experimental design study on waste activated sludge in batch mode. The optimum temperature, reaction time and pH of the process were determined to be 80 °C, 5 h and pH 10, respectively. All three factors had a strong individual effect (p < 0.001), with a significant interaction effect for temp. pH 2 (p = 0.002). Thermal pre-treatments, carried out on seven different municipal wastewater sludges at the above optimum operating conditions, produced increased COD solubilization of 18.3 ± 7.5% and VSS reduction of 27.7 ± 12.3% compared to the untreated sludges. The solubilization of proteins was significantly higher than carbohydrates. Methane produced in biochemical methane potential (BMP) tests, indicated initial higher rates (p = 0.0013) for the thermally treated samples ( k hyd up to 5 times higher), although the ultimate methane yields were not significantly affected by the treatment. [ABSTRACT FROM AUTHOR]

• CA treatment directly on the sludge in anaerobic digestion boosted methane production. • HA deconstruction and redistribution enhance sludge solubilization and hydrolysis. • CA treatment at low dosage can release HA surfactants and proteins e.g. hydrolases. • A smart mutex-switch CA treatment strategy was proposed for practical application. Humic acids were known to inhibit sewage sludge anaerobic digestion (AD) and many studies have been investigated to deal with the inhibitory effect of humic acids (HA) in raw sludge feeding the digestor. However, HA could also be in-situ produced during the sludge AD process with a significant amount, but the relieving of the inhibitory effect posed by the in-situ produced HA has been historically ignored. Thus, this study attempted to use citric acid (CA), an easily biodegradable polyatomic acid, to directly treat sludge in the anaerobic digestor instead of the feeding sludge, for alleviating inhibition caused by the in-situ produced HA. Results showed that methane production of AD batches with CA pretreatment at 0.06∼0.10 g/g TS on digestor sludge were effectively enhanced, with the highest production being 2.89 times of that observed in control AD without CA treatment and 2.08 times of that achieved by contrast AD with CA pretreatment on raw sludge. However, the AD batches with CA treatment at low dosages of 0.02∼0.04 g/g TS had lower methane production, during which sludge solubilization and hydrolysis were enhanced initially associated with released HA surfactant and hydrolytic enzymes; then later inhibited since higher structurally sound HA in SP easily rebind proteinaceous substrates and enzymes again. Whilst CA treatment at 0.06∼0.10 g/g TS could promote sludge solubilization and hydrolysis throughout the sludge AD process. This dosage-dependent effect of CA treatment can be caused by its discriminating impact on HA structure and distribution. At low dosages, complexes of HA-proteins (including hydrolases) can be decrosslinked and transferred to the soluble phase (SP), thus enhancing solubilization and hydrolysis initially. However, the released HA in SP could rebind more organics and hydrolases during AD, resulting in augmented inhibition. While the dosage increased, HA were drastically deconstructed with aromatic cores in SP migrating to extracellular polymeric substances, preventing binding effects on organics and hydrolases. Accordingly, a smart mutex-switch operational strategy of CA treatment was proposed. The results suggested, to alleviate the inhibitory effect of HA on sludge AD, more attention should be given to HA produced and accumulated in AD rather than raw sludge so that the CA treatment directly on sludge in the digestor can be a promising method. [Display omitted] [ABSTRACT FROM AUTHOR]

• High polysaccharide availability determined strong sludge humification in AD. • THP/160 °C promoted polysaccharide availability via decomposition and hydrolysis. • THP/180 °C reduced polysaccharide availability by heterocyclization to melanoidins. • THP influenced both polyphenol synthesis and humus condensation pathways in AD. • Melanoidins distinguishment is vital for humification investigation of THP samples. Humus accumulation during sludge stabilization is attracting increasing attention because of its land use potential. This study investigated the effects of thermal hydrolysis pretreatment (THP) on sludge humification and the related regulatory mechanisms during anaerobic digestion (AD). The sludge subjected to AD with THP at 160 °C (AnD-160) exhibited 7.3% and 8.5% higher total extractable carbon (TEC) and humic acid carbon (HAC) concentrations than the unpretreated sludge, respectively, while the sludge subjected to AD with THP at 180 °C (AnD-180) exhibited 1.4% and 7.0% lower concentrations. Humification of AnD-160 and AnD-180 were enhanced and inhibited, respectively. THP influenced sludge humification during AD through both intracellular polyphenol synthesis and extracellular humus condensation pathways related to polysaccharide metabolism. The 160 °C-pretreated sludge exhibited higher polysaccharide availability (more soluble polysaccharides and reducing sugars) than the other samples and thus had more active intracellular polyphenol metabolism during AD. The polyphenol accumulation stimulated the synthesis of related condensation enzymes (polyphenol oxidase and quinone oxidoreductases), facilitating extracellular HA production during AD. However, THP at 180 °C resulted in the highest production of melanoidins with C O-C heterocycles through C = O and C H sites of polysaccharides (778.46 vs. 193.87 R.U. nm2-mL/g for the unpretreated sample). This heterocyclization decreased the polysaccharide availability and inhibited intracellular polyphenol synthesis, consequently inhibiting condensation enzymes supply and extracellular HA production during AD. Overall, this study highlights the crucial but neglected role of polysaccharides in sludge humification during AD: the availability of polysaccharides affected by their occurrence state and molecular structure could determine the humification, which can be regulated by THP at different temperature. [Display omitted] [ABSTRACT FROM AUTHOR]

The occurrence of emerging organic micropollutants (OMPs) in sewage sludge has been widely reported; nevertheless, their fate during sludge treatment remains unclear. The objective of this work was to study the fate of OMPs during mesophilic and thermophilic anaerobic digestion (AD), the most common processes used for sludge stabilization, by using raw sewage sludge without spiking OMPs. Moreover, the results of analytical chemistry were complemented with biological assays in order to verify the possible adverse effects (estrogenic and genotoxic) on the environment and human health in view of an agricultural (re)use of digested sludge. Musk fragrances (AHTN, HHCB), ibuprofen (IBP) and triclosan (TCS) were the most abundant compounds detected in sewage sludge. In general, the efficiency of the AD process was not dependent on operational parameters but compound-specific: some OMPs were highly biotransformed (e.g. sulfamethoxazole and naproxen), while others were only slightly affected (e.g. IBP and TCS) or even unaltered (e.g. AHTN and HHCB). The MCF-7 assay evidenced that estrogenicity removal was driven by temperature. The Ames test did not show point mutation in Salmonella typhimurium while the Comet test exhibited a genotoxic effect on human leukocytes attenuated by AD. This study highlights the importance of combining chemical analysis and biological activities in order to establish appropriate operational strategies for a safer disposal of sewage sludge. Actually, it was demonstrated that temperature has an insignificant effect on the disappearance of the parent compounds while it is crucial to decrease estrogenicity. [ABSTRACT FROM AUTHOR]

(Hydro)thermal treatments of sewage sludge is a promising option that can simultaneously target safe waste disposal, energy recovery, and nutrient recovery/recycling. The speciation of phosphorus (P) in sludge is of great relevance to P reclamation/recycling and soil application of sludge-derived products, thus it is critical to understand the effects of different treatment techniques and conditions on P speciation. This study systematically characterized P speciation (i.e. complexation and mineral forms) in chars derived from pyrolysis and hydrothermal carbonization (HTC) of municipal sewage sludges. Combined sequential extraction and P K-edge X-ray absorption near edge structure (XANES) spectroscopy analysis revealed the dependence of P transformation on treatment conditions and metal composition in the feedstocks. Pyrolysis of sludges decreased the relative abundance of phytic acid while increased the abundance of Al-associated P. HTC thoroughly homogenized and exposed P for interaction with various metals/minerals, with the final P speciation closely related to the composition/speciation of metals and their affinities to P. Results from this study revealed the mechanisms of P transformation during (hydro)thermal treatments of sewage sludges, and might be applicable to other biosolids. It also provided fundamental knowledge basis for the design and selection of waste management strategies for better P (re)cycling and reclamation. [ABSTRACT FROM AUTHOR]

Understanding which/how antibiotic resistance genes (ARGs) contribute to increased acquisition of resistance by pathogens in aquatic environments are challenges of profound significance. We explored the co-occurrence and removal versus enrichment of ARGs and human bacterial pathogens (HBPs) in municipal sewage sludge digesters. We combined metagenomic detection of a wide spectrum of 323 ARGs and 83 HBPs with a correlation-based statistical approach and charted a network of their co-occurrence relationships. The results indicate that most ARGs and a minor proportion of HBPs (mainly Collinsella aerofaciens, Streptococcus salivarius and Gordonia bronchialis ) could not be removed by anaerobic digestion, revealing a biological risk of post-digestion sludge in disseminating antibiotic resistance and pathogenicity. Moreover, preferential co-occurrence patterns were evident within one ARG type (e.g., multidrug, beta-lactam, and aminoglycoside) and between two different ARG types (i.e., aminoglycoside and beta-lactam), possibly implicating co-effects of antibiotic selection pressure and co-resistance on shaping antibiotic resistome in sewage sludge. Unlike beta-lactam resistance genes, ARGs of multidrug and macrolide-lincosamide-streptogramin tended to co-occur more with HBPs. Strikingly, we presented evidence that the most straightforward biological origin of an ARG-species co-occurring event is a hosting relationship. Furthermore, a significant and robust HBP-species co-occurrence correlation provides a proper scenario for nominating HBP indicators (e.g., Bifidobacterium spp. are perfect indicators of C. aerofaciens ; r = 0.92–0.99 and P-values < 0.01). Combined, this study demonstrates a creative and effective network-based metagenomic approach for exploring ARG hosts and HBP indicators and assessing ARGs acquisition by HBPs in human-impacted environments where ARGs and HBPs may co-thrive. [ABSTRACT FROM AUTHOR]

In this study, laboratory scale digesters were operated to simulate potential shocks to the Anaerobic Digestion (AD) process at a 350 ML/day wastewater treatment plant. The shocks included high (42 °C) and low (32 °C) temperature (either side of mesophilic 37 °C) and a 20% loading of fats, oil and grease (FOG; 20% w:v). These variables were explored at two sludge retention times (12 and 20 days) and two organic loading rates (2.0 and 2.5 kgTS/m 3 day OLR). Metagenomic and metabolomic approaches were then used to characterise the impact of operational shocks in regard to temperature and FOG addition, as determined through monitoring of biogas production, the microbial profile and their metabolism. Results showed that AD performance was not greatly affected by temperature shocks, with the biggest impact being a reduction in biogas production at 42 °C that persisted for 32 ± 1 days. The average biogas production across all digesters at the completion of the experiment was 264.1 ± 76.5 mL/day, with FOG addition observed to significantly promote biogas production (+87.8 mL/day). Metagenomic and metabolomic analyses of the digesters indicated that methanogens and methane oxidising bacteria (MOB) were low in relative abundance, and that the ratio of oxidising bacteria (methane, sulphide and sulphate) with respect to sulphate reducing bacteria (SRB) had a noticeable influence on biogas production. Furthermore, increased biogas production correlated with an increase in short chain fatty acids, a product of the addition of 20% FOG. This work demonstrates the application of metagenomics and metabolomics to characterise the microbiota and their metabolism in AD digesters, providing insight to the resilience of crucial microbial populations when exposed to operational shocks. [ABSTRACT FROM AUTHOR]

• Advanced process control (APC) tools are an alternative for upgrading WWTPs. • Improvement of sludge thickening by APC is key to increase methane yield. • Optimized anaerobic digestion increase WWTP energy self-sufficiency up to 53%. • APC reduction in reagents and in sludge management also reduce CAPEX return. On their path to becoming sustainable facilities, it is required that wastewater treatment plants reduce their energy demand, sludge production, and chemical consumption, as well as increase on-site power generation. This study describes the results obtained from upgrading the sludge line of a full-scale wastewater treatment plant over 6 years (2015–2021) using three advanced process control strategies. The advanced process control tools were designed with the aim of (i) enhancing primary and secondary sludge thickening, (ii) improving anaerobic digestion performance, and (iii) reducing chemical consumption in the sludge line. The results obtained show that the use of advanced process control tools allows for optimising sludge thickening (increasing solids content by 9.5%) and anaerobic digestion (increasing both the removal of volatile solids and specific methane yield by 10%, respectively), while reducing iron chloride and antifoam consumption (by 75% and 53%, respectively). With the strategies implemented, the plant increased its potential energy self-sufficiency from 43% to 51% and reduced de-watered sludge production by 11%. Furthermore, the upgrade required a low investment, with a return of capital expense (CAPEX) in 1.98 years, which presents a promising and affordable alternative for upgrading existing wastewater treatment plants. [Display omitted] [ABSTRACT FROM AUTHOR]

Large amount of sludge has been a great trouble and raised significant concerns in China. This paper reviewed the current situation of sludge production, management, treatment and disposal in China. Total sludge production in China had an average annual growth of 13% from 2007 to 2013, and 6.25 million tons dry solids was produced in 2013. Per Capita sludge production in China is lower than that in developed countries. However, sludge management is poor in China. Administrative agents of sludge are not in accordance with each other. Laws and regulations of sludge management are incomplete and sometimes unrealistic. As to sludge treatment and disposal, many technical routes have been applied in China. Thickening, conditioning, and dewatering are three most used treatment methods, while application ratios of stabilization and drying are low in China. More than 80% of sludge is disposed by improper dumping in China. Regarding proper disposal, sanitary landfill is the commonest, followed by land application, incineration and building materials. According to the overall situation of China, “thickening-anaerobic digestion-dewatering-land application” is the priority technical route of sludge treatment and disposal. Good changes, current challenges and future perspectives of this technical route in China were analyzed and discussed in details. [ABSTRACT FROM AUTHOR]

Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances. In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate. 16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process. The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions. [ABSTRACT FROM AUTHOR]

In this study, the anaerobic digestion of thermally hydrolyzed wasted sludge (THWS) with a high concentration of ammonia was carried out through combining with an ammonia stripping and an electrolyzed water system (EWS). The EWS produced acidic water (pH 2–3) at the anode and alkaline water (pH 11–12) at the cathode with an electro-diaphragm between the electrodes that could be applied to ammonia stripping. The ammonia stripping efficiency was strongly dependent on the pH and aeration rate, and the ammonium ion removal rate followed pseudo-first-order kinetics. From the BMP test, the methane yield of THWS after ammonia stripping using the EWS was 2.8 times higher than that of the control process (raw THWS without ammonia stripping). Furthermore, both methane yield and ammonium removal efficiency were higher in this study than in previous studies. Since ammonia stripping with the EWS does not require any chemicals for pH control, no precipitated sludge is produced and anaerobic microorganisms are not inhibited by cations. Therefore, ammonia stripping using the EWS could be an effective method for digestion of wastewater with a high concentration of ammonium nitrogen. [ABSTRACT FROM AUTHOR]

Anaerobic codigestion (AcoD) is a proven option to significantly boost biogas production while utilizing existing digesters and infrastructure. The aim of the present research was to conduct an exhaustive study regarding anaerobic codigestion of mixed sewage sludge and crude glycerol considering impacts on organic load, hydraulic load, process performance and microbial community. The methane potential of crude glycerol varied from 370 mL CH 4 ·g −1 VS to 483 mL CH 4 ·g −1 VS for different samples tested. The half maximal inhibitory concentration of crude glycerol was 1.01 g VS L −1 , and the primary mechanism of inhibition was through overload from rapid fermentation rather than the presence of toxic compounds in the crude glycerol. In continuous operation over 200 days, feeding glycerol at up to 2% v/v, increased organic load by up to 70% and resulted in a 50% increase in methane production. Glycerol dosing resulted in no change in apparent dewaterability, with both codigestion and control reactors returning values of 22%–24%. Members of the phylum Thermotogae emerged as a niche population during AcoD of sewage sludge and glycerol; however there was no gross change in microbial community structure and only minimal changes in diversity. AcoD did not result in synergisms between sewage sludge and crude glycerol. Actually, at dose rate up to 2% v/v glycerol dosing is still an effective strategy to increase the organic loading rate of continuous anaerobic digesters with minimal impact of the hydraulic retention time. Nonetheless, the dose rate must be managed to: (i) prevent process inhibition and (ii) ensure sufficient degradation time to produce a stable biosolids product. [ABSTRACT FROM AUTHOR]

Filamentous bacteria such as Microthrix parvicella can cause serious foaming and floating sludge problems in anaerobic digesters fed with sewage sludge. The sewage sludge and oil co-fermenting laboratory-scale biogas digesters in this study were fed with substrates from a foaming-prone full-scale biogas plant containing the filamentous bacterium M . parvicella . At 37 °C, in both pneumatically mixed digesters a highly viscous and approximately 3 cm thick floating sludge was observed. A gradual increase of the temperature from 37 °C to 56 °C led to a significant decrease in the floating sludge thickness, which correlated with a strong decrease in the abundance of M. parvicella in the digestate. Furthermore, the stepwise temperature increase allowed for an adaption of the microbial community and prevented process failure. The study indicates that already a moderate temperature increase from 37 °C to 41 °C might help to control the M. parvicella abundance in full-scale biogas plants. [ABSTRACT FROM AUTHOR]

Nonylphenol (NP) compounds are toxic and persistent chemicals that are not fully degraded either in natural or engineered systems. Current knowledge indicates that these compounds concentrate in sewage sludge. Therefore, investigating the degradation patterns and types of metabolites formed during sludge treatment are important for land application of sewage sludge. Unfortunately, the information on the fate of nonylphenol compounds in sludge treatment is very limited. This study aims to investigate the biodegradation patterns of nonylphenol diethoxylate (NP2EO) in aerobic batch digesters. For this purpose, two NP2EO spiked and two control laboratory aerobic batch digesters were operated. The spiked digester contained 3 mg/L NP2EO in the whole reactor content. The compounds of interest (parent compound and expected metabolites) were extracted with sonication and analyzed by gas chromatography-mass spectrometry (GC–MS) as a function of time. Results showed that, following the day of spike, NP2EO degraded rapidly. The metabolites observed were nonylphenol monoethoxylate (NP1EO), NP and dominantly, nonylphenoxy acetic acid (NP1EC). The mass balance over the reactors indicated that the total mass spiked was highly accounted for by the products analyzed. The time dependent analysis indicated that the parent compound degradation and daughter product formation followed first order kinetics. The digester performance parameters analyzed (VS and COD reduction) indicated that the spike of NP2EO did not affect the digester performance. [ABSTRACT FROM AUTHOR]

• IntensiCarbTM (IC) is a novel technology that allows simultaneous thickening and fermentation in a single treatment step. • IC improved both VFA and hydrolysis yields compared to control fermenter. • IC produced condensate at higher quality with no solids and low nutrient contents. • Applying vacuum to the fermenter leads to doubling the volumetric organic loading. • Applying vacuum has no negative impact on fermentative bacteria and methanogens. This study assessed the feasibility of a novel vacuum-enhanced anaerobic digestion technology, referred to as IntensiCarbTM (IC), under mild vacuum pressure (110 mbar), compared to a control (conventional fermenter), and evaluated the impact of the vacuum on the activities of various microbial groups. Both fermenters (test and control) were operated with mixed (50% v/v) municipal sludge at solids concentrations of 2–2.5%, pH of 7.8–8.1, 40–45 °C, a theoretical solids retention time (SRT) of 3 days with different hydraulic retention times (HRT). The intensification factor (IF) of the IC, defined as SRT/HRT, was controlled at 1.3 and 2.0. Simultaneous thickening and fermentation intensification were achieved. Compared with the control, the IC, despite the shorter HRTs, achieved 29.5 to 90.2% increase in the VFA yield (79 to 116 mg ΔVFA/ g VSS vs 61 mg ΔVFA/ g VSS), and 16.2% to 56.4% increase (280 to 377 mg ΔsCOD/ g VSS vs 241 mg ΔsCOD/ g VSS), in the hydrolysis yield. Fermentate from the IC exhibited comparable specific denitrification rates to acetate. Further, the solids-free condensate contained low nutrient concentrations, and thus was far superior to a typical centrates from dewatering as a carbon source. No adverse effects of vacuum on the activity of fermentative bacteria and methanogens were observed. This study demonstrated that the IC can be deployed as an intensification technology for both fermentation and anaerobic digestion of biosolids with the additional significant advantage, i.e. elimination of sidestream ammonia treatment requirements. [Display omitted] [ABSTRACT FROM AUTHOR]

• A novel green magnetic straw-based biochar (MSBC) was prepared. • MSBC enhanced volatile fatty acid and methane production from sludge. • MSBC promoted the intra- and extracellular electron transfer efficiency. • MSBC improved acetoclastic methanogenesis and direct interspecies electron transfer. Anaerobic digestion (AD) of waste activated sludge (WAS) is usually limited by the low generation efficiency of methane. The addition of composite conductive materials (CMs) is a promising strategy to enhance AD performance. In this study, a new green magnetic-straw-based biochar (MSBC) was synthesised by a simple ball-milling/carbonisation method, and its effects on AD performance of sludge were investigated. Experimental results showed that the as-synthesised MSBC had an intrinsic graphene-oxide-like structure, with Fe species serving as electroactive sites; these characteristics translate into a high electron transfer (ET) capability. After adding MSBC, the volatile fatty acid production and methane yield were significantly increased by 14.13% and 45.36%, respectively. Analysis of the changes in the ET system activities, hydrogenase activities, Cyt-C concentrations and the electron transfer capacity of the sludge sample with and without the MSBC revealed that the MSBC enhanced intracellular ET and changed the extracellular ET pathway from indirect interspecies hydrogen transfer to direct interspecies electron transfer (DIET), which would be responsible for increasing methane production and proportion in the biogas. However, further analyses of key enzyme activities and the microbial community indicated that the MSBC reinforces the methanogenesis pathway by creating a favourable environment (i.e., by enhancing hydrolysis-acidification and DIET-based CO 2 reduction) for acetoclastic methanogens. These findings, however, are expected to provide an important reference for developing CMs application in AD. [Display omitted] [ABSTRACT FROM AUTHOR]

• Anaerobic digestion (AD) with isoelectric-point (pI) pretreatment was evaluated. • pI pretreatment shortened solids retention time (SRT) of AD of sludge to 5 days. • pI pretreatment promoted methane yield and its content in biogas at different SRTs. • pI pretreatment improved the economic and environmental benefits from AD of sludge. The prolonged reaction period and low methane yield have become a pivotal bottleneck in the anaerobic digestion (AD) of waste activated sludge (WAS), severely limiting its use in bioenergy. This study evaluated the long-term semi-continuous AD of WAS with isoelectric-point (pI) pretreatment in terms of the reaction period, methane yield, material resource recovery, and economic and environmental benefits. The experimental results show that after pI pretreatment, at the same solids retention time (SRT), the average methane yield and methane content in the biogas of WAS increased by 120.9% and 15.5%, respectively. The average methane yield from AD with pI pretreatment at a 5-day SRT was 1.6 times that of the AD without pI pretreatment at a 15-day SRT, indicating that pI pretreatment significantly (P < 0.001) increased the methane yield from the AD of WAS at different SRTs, even at an ultra-short SRT. Statistical analyses of the changes in the concentrations of soluble organic matter in the AD process with and without pI pretreatment confirmed that pI pretreatment enhanced the biochemical reactions related to the transformation of sludge organic matter during the whole AD process, but not the initial organic solubilisation and hydrolysis of the sludge. Furthermore, pI pretreatment recovered the NaHCO 3 , Na 2 S, and multivalent metals from the sludge, indicating that AD with pI pretreatment efficiently recovered both bioenergy and material resources from the sludge. Economic and environmental analyses of AD with and without pI pretreatment further indicated that with pI pretreatment, the annual economic benefit of AD increases by 162% and carbon emissions decreases by approximately 2.63 × 107 kg CO 2 /year compared with conventional AD. These findings serve as an important reference for the development of a novel AD technology for sludge that incorporates second-generation pretreatment and operates at an ultra-short SRT. [Display omitted] [ABSTRACT FROM AUTHOR]

• Anaerobic digestion (AD) of sludge with MOF-808 was investigated. • MOF-808 enhanced methane production and its content in biogas from AD of sludge. • MOF-808 improved the hydrolysis degree and rate of sludge organic matters. • MOF-808 accelerated the bioconversion of intermediate products to methane. • MOF-808 promoted the proton transfer and changed the methanogenic pathway. The bioconversion of waste activated sludge (WAS) into methane is usually limited by the poor hydrolysis of sludge and/or poor syntrophic methanogenesis during anaerobic digestion (AD). In this study, the underlying mechanism of MOF-808 enhancing hydrolysis and syntrophic methanogenesis during AD process of WAS was investigated. Experimentally, with the effects of MOF-808 (150 mg MOF-808/g Volatile Solid (VS)), the methane production and the proportion of methane in biogas increased by approximately 26.7% and 15.6%, respectively, and the lag phase of methanogenesis decreased by 50.8%, which indicate that MOF-808 enhanced the generation efficiency of methane. The changes in activities of main hydrolytic enzymes with and without MOF-808 (150 mg MOF-808/g VS) during AD process revealed that MOF-808 improved the enzymatic hydrolysis of sludge, and the abiotic hydrolysis of sludge extracellular organic substances by MOF-808 shows that the maximum proportion and the initial increasing rate of low-molecular weight fractions increased by 60% and 583.7%, respectively, indicating that MOF-808 can greatly enhance the hydrolysis degree and rate of sludge via abiotic effect. These demonstrate that MOF-808 enhanced both biological and abiotic hydrolysis of sludge during AD. In addition, changes in the concentrations of acetate kinase and volatile fatty acids (VFAs) with and without MOF-808 (150 mg MOF-808/g VS) during AD process showed that MOF-808 accelerated the bioconversion of VFAs to methane, suggesting MOF-808 has a positive effect on syntrophic metabolism for methanogenesis. Moreover, further analyses of the microbial community structure of sludge samples with and without MOF-808 (150 mg MOF-808/g VS) showed that MOF-808 enriched hydrogen-producing bacteria and mixotrophic methanogens (i.e. Methanosarcina), and changed the methanogenic pathway via accelerating proton transfer between syntrophic anaerobes, especially improving the reduction of CO 2 to methane, and resulting in highly efficient syntrophic methanogenesis. These findings, however, may provide an important reference for enhancing AD efficiency of WAS based on MOF-like materials. [Display omitted] [ABSTRACT FROM AUTHOR]

• FW:FSS ratios caused large variations of microbial community composition and populations dynamics. • Traceable microbial clustering was shaped by different FW:FSS ratios. • Methanogenic community altered its trophic groups and energy conserving strategy to ensure methane generation. • Microbial symbiosis and interspecies competition were observed in thermophilic microbiome. A self-stabilizing microbial community lays the foundation of the efficient biochemical reactions of the anaerobic digestion (AD) process. Despite extensive profiling of microbial community dynamics under varying operating parameters, the effects of food waste (FW) to feeding sewage sludge (FSS) ratios on the microbial assembly, functional traits, and syntrophic interspecies interactions in thermophilic microbial consortia remain poorly understood. Here, we investigated the long-term impacts of the FW: FSS ratio on the thermophilic AD microbiome using genome-centric metagenomics. Both the short reads (SRs) assembly, and the iterative hybrid assembly (IHA) of SRs and nanopore long reads (LRs) were used to reconstruct metagenome-assembled genomes (MAGs) and four microbial clusters were identified, demonstrating different microbial dynamics patterns in response to varying FW:FSS ratios. Cluster C1-C3 were comprised of full functional members with genetic potentials in fulfilling empirical AD biochemical reactions, wherein, syntrophic decarboxylating acetogens could interact with methanogens, and some microbes could be energized by the electron bifurcation mechanism to drive thermodynamics unfavorable reactions. We found the co-existence of both acetogenic and hydrogenotrophic methanogens in the AD microbiome, and they altered their trophic groups to scavenge the methanogenic substrates in ensuring the methane generation in digesters with different FW:FSS ratios. Another interesting observation was that two phylogenetically close Thermotogota species showed a possible strong competition on carbon source inferred by the nearly complete genetic overlap of their relevant pathways. : [Display omitted] [ABSTRACT FROM AUTHOR]

• Light exposure enhances the performance for mesophilic digestion of sludge. • Light exposure significantly promotes electrical conductivity of biomass. • The component of TB-EPS is more affected than those of LB-EPS in light conditions. • Light exposure alters the combination of DIET microbes on the carbon felt. • Higher EEC of EPS is conducive to DIET process under light conditions. Light as an environmental factor can affect the process of anaerobic digestion, but there is no systematic study in municipal wastewater sludge mesophilic digestion. In this study, the effects of light on the performance of the anaerobic digestion system and photo-anaerobic microbiota (PAM) were evaluated in lighted anaerobic batch digesters (LABRs). The methane yield from the reactor under the dark condition (LABR0) was 179.2 mL CH 4 /g COD, which was lower than 305.4 mL CH 4 /g COD and 223.0 mL CH 4 /g COD (n = 3, p < 0.05) from reactors under the light intensity of 3600 lm (LABR1) and 7200 lm (LABR2), respectively. The dominant genera in the bacterial and archaeal communities were Bacillus and Methanosarcina under light conditions, Enterococcus and Methanobacterium under dark conditions. And these two bacteria acted as electroactive bacterial genera, indicating that light changes the combination of direct interspecies electron transfer (DIET) microbial partners and activates the DIET pathway for methane production. The electron conduction pathways analysis further suggests that biological DIET (bDIET) between microbial biomass, rather than DIET via conductive material (cDIET) between microbes and conductive materials, is promoted and behaves as the dominant factor enhancing methane production under light conditions. The morphology of microorganisms and the amount and properties of EPS corroborate these views. Our findings are guided to anaerobic digester constructions under the outdoor environment with light exposure. [Display omitted] [ABSTRACT FROM AUTHOR]

• MFs increase the abundances of eARGs during sludge anaerobic digestion. • The increase of eARGs release by MFs has no significant selectivity. • The potential host number of eARGs also increased with MFs exposure. • Increased eARGs release could attribute to the upregulated type IV secretion system. • Increased eARGs may promote ARGs spread and should be considered in sludge handling. Microplastic fibers (MFs), as the most frequently detected microplastic shape in sewage sludge, have posed emerging concern for sludge treatment and disposal. However, the effect of MFs on antibiotic resistance genes (ARGs), especially extracellular ARGs (eARGs) during sludge treatment remains far from explicit. Therefore, this study investigated the potential impact of MFs on eARGs during sludge anaerobic digestion (AD), a commonly used sludge treatment method, through long-term operation. The qPCR results showed that both absolute and relative abundances of eARGs increased with the MFs exposure during sludge AD. The average absolute and relative abundances of eight tested eARGs in the AD reactor with the highest MFs dosage (170 items/gTS) were 1.70 and 2.15 times higher than those in the control AD reactor. The metagenomics results further comfirmed the increase of eARGs abundance during sludge anaerobic digestion after MFs exposure and the enhancement did not show significant selectivity. The identification of the potential hosts of eARGs suggested the host numbers of eARGs also increased with MFs exposure, which may suggest enhanced horizonal transformation as a result of increased eARGs. Further exploring the mechansims showed that the genes involved in type IV secretion system was upregulated after MFs exposure, suggesting the active release of eARGs was enhanced with MFs exposure. In contrast, the MFs may not affect the passive release of eARGs as its impact on cell membrance damage was insignificant. The enhanced eARGs in sludge AD process may further accelerate the transport of ARGs in environment, which should be considered during sludge treatment and disposal. [Display omitted] [ABSTRACT FROM AUTHOR]

• Mass balance of 11 PFAAs in a laboratory wastewater system was investigated. • Total mass of PFAAs increased by 112% in the system. • Mass increase of PFAAs: activated sludge > sewer process > anaerobic digestion. • Mass of 11 PFAAs in the effluent was 5 times higher than that in digested sludge. The fate and formation of perfluoroalkyl acids (PFAAs) have been investigated during wastewater treatment processes but studies for the entire urban wastewater system comprising the sewage transport and wastewater and sludge treatment processes are scarce. This work performs an integrated assessment of the formation and fate of PFAAs in the urban wastewater system together with their behavior in separate components of the system. To achieve this, PFAAs were monitored over five weeks in a laboratory-scale urban wastewater system comprising sewer reactors, a wastewater treatment reactor, and an anaerobic sludge digester. The system was fed with real domestic wastewater. The total mass of 11 PFAAs flowing out of the laboratory wastewater system significantly (p < 0.05) increased by 112 ± 14 (mean ± standard error)% compared to that entering the system. Formation of PFAAs was observed in all three biological processes of the system. In anaerobic sewer process, perfluoropentanoic acid (PFPeA), perfluoroheptanoic acid (PFHpA), and perfluorooctane sulfonate (PFOS) exhibited significant formation (p < 0.05) with the mass flow increased by 79 ± 24%, 109 ± 31%, and 57 ± 17%, respectively. During the wastewater treatment process, perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), and perfluorododecanoic acid (PFDoDA) demonstrated significant increase (p < 0.05) in their mass flows by 176 ± 56%, 92 ± 21%, and 516 ± 184%, respectively. In contrast, only PFHxA was found to significantly (p < 0.05) increase by 130 ± 40% during anaerobic digestion process. The total mass of 11 PFAAs discharged through the effluent (201 ± 24 ng day−1) was 5 times higher than that through the digested sludge (29 ± 6 ng day−1). [Display omitted] [ABSTRACT FROM AUTHOR]

Antibiotic resistance has become a serious threat to human health. Sewage treatment plant (STP) is one of the major sources of antibiotic resistance genes (ARGs) in natural environment. High-throughput sequencing-based metagenomic approach was applied to investigate the broad-spectrum profiles and fate of ARGs in a full scale STP. Totally, 271 ARGs subtypes belonging to 18 ARGs types were identified by the broad scanning of metagenomic analysis. Influent had the highest ARGs abundance, followed by effluent, anaerobic digestion sludge and activated sludge. 78 ARGs subtypes persisted through the biological wastewater and sludge treatment process. The high removal efficiency of 99.82% for total ARGs in wastewater suggested that sewage treatment process is effective in reducing ARGs. But the removal efficiency of ARGs in sludge treatment was not as good as that in sewage treatment. Furthermore, the composition of microbial communities was examined and the correlation between microbial community and ARGs was investigated using redundancy analysis. Significant correlation between 6 genera and the distribution of ARGs were found and 5 of the 6 genera included potential pathogens. This is the first study on the fate of ARGs in STP using metagenomic analysis with high-throughput sequencing and hopefully would enhance our knowledge on fate of ARGs in STP. [ABSTRACT FROM AUTHOR]

• An urban mining concept is introduced to recover phosphorus from digested sludge. • High gradient magnetic separators (HGMS) can recover vivianite from digested sludge. • A pilot scale HGMS showed a vivianite recovery of 80% from digested sludge. • Vivianite recovery allows for 60% recovery of phosphorus from influent wastewater. • The concentrate had a vivianite content of 0.8 g/g and low heavy metal content. Phosphorus (P) is an essential resource for food production and chemical industry. Phosphorus use has to become more sustainable and should include phosphorus recycling from secondary sources. About 20% of P ends up in sewage sludge, making this a substantial secondary P source. There is currently a technological gap to recover P from sludge locally at wastewater treatment plants (WWTP) that remove P by dosing iron. Vivianite (Fe 3 (PO 4) 2 •8(H 2 O)) is the main iron phosphate mineral that forms during anaerobic digestion of sewage sludge, provided that enough iron is present. Vivianite is paramagnetic and can be recovered using a magnetic separator. In this study, we have scaled up vivianite separation from lab-scale to bench- and pilot-scale. Bench-scale tests showed good separation of vivianite from digested sewage sludge and that a pulsation force is crucial for obtaining a concentrate with a high P grade. A pilot-scale magnetic separator (capacity 1.0 m3/h) was used to recover vivianite from digested sewage sludge at a WWTP. Recirculating and reprocessing sludge allows over 80% vivianite recovery within three passes. A concentrated P-product was produced with a vivianite content of up to 800 mg/g and a P content of 98 mg/g. P recovery is limited by the amount of P bound in vivianite and can be increased by increased iron dosing. With sufficient iron dosing, the vivianite content can be increased, and subsequently more P can be recovered. This would allow compliance with existing German legislation, which requires a P recovery larger than 50%. [Display omitted] [ABSTRACT FROM AUTHOR]

• Cation exchange resin (CER) pretreatment removed organic-binding metals from sludge • CER pretreatment collapsed stability of extracellular polymeric substances • CER pretreatment promoted solubilisation, hydrolysis, acidification and methanation • CER pretreatment enhanced the proportion of methane in the biogas • CER pretreatment enriched the methylotrophic methanogen The application of anaerobic digestion (AD) to treat waste activated sludge (WAS) still exhibits some limitations, such as low methane production. In this study, cation exchange resin (CER) pretreatment was explored to enhance the efficiency of the AD of WAS. Based on the response surface methodology, the optimal conditions for CER pretreatment were reaction time of 7.4 h, 33.8 g CER (wet weight) /g volatile solids and sludge total solids of 2.4%. Under these optimal CER pretreatment conditions, approximately 30% of metals were removed from the WAS, particularly organic-binding metals. This metal removal disrupted the structures of extracellular polymer substances and led to sludge deflocculation, thereby releasing large amounts of organic substances from the sludge solids. Batch AD experiments showed that CER pretreatment increased the maximal production of volatile fatty acids and methane by 565.7% and 80.5%, respectively. Additionally, CER pretreatment promoted each stage of AD (i.e. solubilisation, hydrolysis, acidification and methanation) and the corresponding activities of key enzymes. Experimental results for semi-continuous AD further confirmed that CER pretreatment enhanced the proportion of methane in the biogas (from 62.75 ± 2.14% to 73.96 ± 0.99%) and the production of methane. An analysis of changes in the microbial communities demonstrated that CER pretreatment enhanced the abundance of microorganisms involved in hydrolysis, acidification and acetification and changed the major methanogenic pathway from acetoclastic methanogens to methylotrophic methanogens. These findings are expected to provide a reference for developing new pretreatment methods for enhancing anaerobic biodegradability of organic matters. [Display omitted] [ABSTRACT FROM AUTHOR]

• Large amounts of HAs were released from sludge during THP. • Structural characteristics of HAs were altered during THP. • Influences of HAs on sludge digestion varied with the concentration and structure. • HAs inhibited sludge hydrolysis but improved methanogenesis at low concentration. Humic-like acids (HAs) are abundant in sewage sludge but mainly bonded with solids. Thus, their influences are often neglected in conventional digestion processes. Currently thermal hydrolysis pretreatment (THP) has been widely adopted in sludge anaerobic digestion (AD) to enhance hydrolysis of complex matters and further to improve methane production. However, the impacts of enhanced release of HAs and the mechanisms involved are not well understood and need to be further investigated because the substantial amounts of HAs present in AD could severely threaten the sludge AD processes. Results in the present study indicated that the concentration of soluble HAs in sludge was elevated by 90 times due to the THP, from 8 mg/L in raw sludge to 727 mg/L in the pretreated sludge hydrolyzed at 180 °C. Moreover, the structural characteristics of soluble HAs, including aromatic condensation degree, elemental composition and functional group, also showed substantial differences with the increased temperature of the THP. Furthermore, the release of HAs presented significant influences on sludge digestion. Acidification rate was inhibited by over 50% with 0.4 g/L of HAs, whereas methanogenesis was improved by nearly 200% with 0.8 g/L HAs and inhibited about 50% with 2.0 g/L. The activities of proteinase and co-enzyme F 420 were decreased by 20% and increased by 19%, respectively, under HAs stress at 0.6 g/L for 5 days. Moreover, molecular structural changes of soluble HAs also contributed to the influences. Especially, the E4/E6 value representing the degree of HAs aromatic condensation and C/N ratio of soluble HAs were closely correlated with their inhibition degree to sludge hydrolysis. The findings of this study demonstrate that the influences of HAs are evident and also vary to the different steps of anaerobic digestion processes, which shall not be negligible during the sludge digestion that is with THP. Due to the rate-limiting step was methanogenesis in the AD process of pretreated sludge by thermal hydrolysis, HAs concentration was recommended at low level, for example around 1.0 g/L, to accelerate or not limit methanogenesis. [Display omitted] [ABSTRACT FROM AUTHOR]

• Integration of HTC to biogas plant enhances biogas production and ammonium recovery. • Hydrochar can serve as a combustible or as a phosphorous source in agriculture. • Pharmaceuticals in sewage sludge digestate reduced below detection limit after HTC. • Heavy metals concentrated in hydrochar but were below legislative limits. This study aimed to assess the role of hydrothermal carbonisation (HTC) in digestate processing in centralised biogas plants receiving dewatered sludge from regional wastewater treatment plants and producing biomethane and fertilisers. Chemically conditioned and mechanically dewatered sludge was used as such (total solids (TS) 25%) or as diluted (15% TS) with reject water in 30 min or 120 min HTC treatments at 210 °C, 230 °C or 250 °C, and the produced slurry was filtered to produce hydrochars and filtrates. The different hydrochars contributed to 20–55% of the original mass, 72–88% of the TS, 74–87% of the energy content, 71–92% of the carbon, above 86% of phosphorous and 38–64% of the nitrogen present in the original digestates. The hydrochars' energy content (higher heating values were 11.3–12.2 MJ/kg-TS) were similar to that of the digestates, while the ash contents increased (from 43% up to 57%). HTC treatments produced filtrates in volumes of 42–76% of the dewatered digestate, having a soluble chemical oxygen demand (SCOD) of 28–44 g/L, of which volatile fatty acids (VFAs) contributed 10–34%, and methane potentials of 182–206 mL-CH 4 /g-SCOD without any major indication of inhibition. All 32 pharmaceuticals detected in the digestates were below the detection limit in hydrochars and filtrates, save for ibuprofen and benzotriazole in filtrate, while heavy metals were concentrated in the hydrochars but below the national limits for fertiliser use, save for mercury. The integration of HTC to a centralised biogas plant was extrapolated to enhance the annual biogas production by 5% and ammonium recovery by 25%, and the hydrochar was estimated to produce 83 GJ upon combustion or to direct 350 t phosphorous to agriculture annually. [Display omitted] [ABSTRACT FROM AUTHOR]

• A novel strategy to generation efficiency of methane from sludge is proposed. • Isoelectric-point (pI) pretreatment enhanced interfacial hydrophobic attractions. • pI pretreatment increased abiotic forces driving interfacial enzymatic reactions. • pI pretreatment promoted the electron transfer efficiency in sludge. • pI pretreatment improved the abundances of hydrogenotrophic methanogens. The usefulness of waste activated sludge (WAS) as an energy source is limited by the poor generation efficiency of methane from WAS, which is mainly due to the complex nature of sludge and low abundance of functional microbes. In this study, the interfacial thermodynamics, electron transfer and microbial community of sludge were investigated to reveal the enhancing effects of isoelectric-point (pI) pretreatment on the efficiency of methane generation from WAS. Experimentally, after pI pretreatment, the methane production potential, maximum methane production rate and maximum methane proportion in the biogas increased by 122.2%, 154.4% and 17.4%, respectively, indicating that pI pretreatment enhanced the generation efficiency of methane. Analyses of changes in the solid-liquid interfacial non-covalent interaction energy, electron transfer capacity (ETC) and reductive peak potential values of sludge samples with and without pI pretreatment during a 170-day methanogenic fermentation period revealed that pI pretreatment enhanced the self-driven solid-liquid interfacial hydrophobic attractions of sludge, increased the abiotic driving forces of interfacial enzymatic reactions, promoted the electron transfer efficiency and lowered the barrier of the reduction reaction. It was thus hypothesised that these changes would be responsible for increasing methane production, which was confirmed by the correlation analyses between the interfacial free energy (IFE) and ETC versus daily methane production. Moreover, statistical analyses of the differences between the microbial communities of sludge samples with and without pI pretreatment during fermentation demonstrated that pI pretreatment significantly (P < 0.05) improved the relative abundances of the main functional microbes with respect to hydrolysis, acidification and methanation. A further investigation of the relationships of IFE and ETC with the relative abundances of the main genera of methanogens indicated that the hydrophobic attraction of sludge surface and a high ETC are conducive to the enrichment of hydrogenotrophic methanogens (+29.9%). These findings are expected to provide a conceptual framework for developing second-generation pretreatment methods and provide a methodological reference for revealing the details of the 'black-box' anaerobic digestion process. [Display omitted] [ABSTRACT FROM AUTHOR]

• Performance overview of full-scale sewage sludge mesophilic and thermophilic AD • Species-level identification of microbiota with ecosystem-specific MiDAS3 database • More than 60% of the taxa accounting for 40% of biomass did not grow in the AD • Growing bacteria abundance correlated with mesophilic AD parameters and feed sludge • PLS regression revealed potential ecological function of many uncharacterized taxa Anaerobic digestion (AD) is a key technology at many wastewater treatment plants (WWTPs) for converting primary and surplus activated sludge to methane-rich biogas. However, the limited number of surveys and the lack of comprehensive datasets have hindered a deeper understanding of the characteristics and associations between key variables and the microbial community composition. Here, we present a six-year survey of 46 anaerobic digesters, located at 22 WWTPs in Denmark, which is the first and largest known study of the microbial ecology of AD at WWTPs at a regional scale. For three types of AD (mesophilic, mesophilic with thermal hydrolysis pretreatment, and thermophilic), we present the typical value range of 12 key parameters including operational variables and performance parameters. High-resolution bacterial and archaeal community analyses were carried out at species level using amplicon sequencing of >1,000 samples and the new ecosystem-specific MiDAS 3 reference database. We detected 42 phyla, 1,600 genera, and 3,584 species in the bacterial community, where 70% of the genera and 93% of the species represented environmental taxa that were only classified based on MiDAS 3 de novo placeholder taxonomy. More than 40% of the bacterial species were found not to grow in the mesophilic and thermophilic digesters and were only present due to immigration with the feed sludge. Ammonium concentration was the main driver shaping the bacterial community while temperature and pH were main drivers for the archaea in the three types of ADs. Sub-setting for the growing microbes improved significantly the correlation analyses and revealed the main drivers for the presence of specific species. Within mesophilic digesters, feed sludge composition and other key parameters (organic loading rate, biogas yield, and ammonium concentration) correlated with specific growing species. This survey provides a comprehensive insight into community structure at species level, providing a foundation for future studies of the ecological significance/characteristics and function of the many novel or poorly described taxa. Image, graphical abstract [ABSTRACT FROM AUTHOR]

• cPAM had no negative effects on methane production from SS with and without HTT • The degradability of cPAM was increased from 7.4% without HTT to 76.3% with HTT • HTT decreased the molecular weight of cPAM • The degradation products of cPAM during HTT were identified • HTT resulted in the production of easily biodegradable organics from cPAM Cationic polyacrylamide (cPAM) is a widely used flocculant to dewater sewage sludge (SS) for high-solids anaerobic digestion (AD), and its degradation is crucial since it would release toxic acrylamide (AM) once entering environment. Hydrothermal treatment (HTT) is an efficient method to enhance the AD efficiency of SS. However, the effects of cPAM on AD of SS and the degradation of cPAM during HTT-AD process have not be studied. The study showed cPAM at 20 mg/g TS increased methane yield of SS from 127.0 to 138.9 ml CH 4 /g TS in HTT-AD process, and the biodegradability of cPAM was 76.3%, which was much higher than that (7.4%) without HTT. In HTT-AD process, the enrichment of certain microbes (e.g. Gelria sp.) was observed, which might be related with cPAM degradation. HTT decreased the molecular weight (MW) of cPAM, and resulted in the production of 2-hydroxy-ethyl-trimethylammonium, ammonia, trimethylamine, and ethanol. Methane potential tests of the main HTT products also showed they were easily to be degraded. Overall, HTT-AD integrated process was an efficient method to reduce environmental risk of cPAM as well as increase energy output (biogas), and the study also provided insights into the degradation mechanism of cPAM during HTT. Image, graphical abstract [ABSTRACT FROM AUTHOR]

• A novel strategy to improve volatile fatty acid (VFA) yield from sludge is proposed. • The isoelectric-point (pI) pretreatment loosens the physical structure of sludge. • The pI pretreatment promotes the spatial extension of biopolymer's chains in sludge. • The pI pretreatment increases the driving forces of interfacial enzymatic reactions. • The loose physical structure and hydrophobic surface of sludge facilitate VFA yield. The poor biodegradability of waste activated sludge (WAS) is widely regarded as one of the main bottlenecks in the fermentation of sludge and is attributed mainly to the complex nature of sludge. In this study, the physical structure and interfacial thermodynamics of sludge, which reflect its complex nature, were explored to reveal the effects of isoelectric-point (pI) pretreatment on enhancing the production of volatile fatty acids (VFA). It was observed that the maximum VFA production and the initial VFA production rate increased by 151.2% and 46.6%, respectively, after pI pretreatment, which indicates that pI pretreatment significantly improved the generation efficiency of VFA. The experimental results of 12-day acidogenic fermentation assays following pI pretreatment show that the maximum concentrations of soluble total organic carbon, soluble protein and soluble polysaccharide increased by 209.8%, 148.9% and 84.5%, respectively, and the maximal proportion of low molecular weight (<1 kDa) soluble organic substances increased by 92.4%, thus confirming that pI pretreatment can promote organic solubilisation and hydrolysis in sludge. The analyses of changes in the fractal dimension (D f), the spatial configuration of extracellular polymeric substances, and the interfacial non-covalent interaction energy of sludge during the fermentation process reveal that pI pretreatment can loosen the physical structure, promote the spatial extension of biopolymer molecular chains, and increase the driving forces of solid-liquid interfacial enzymatic reactions. It is thus hypothesised that these changes could be responsible for the high degree of organic solubilisation, hydrolysis and acidification of WAS, which is further confirmed by correlation analyses of the D f and interfacial free energy versus VFA production. These findings are expected to provide a possible means to improve the biodegradability of sludge via its pI to trigger dismantling of the sludge structure and increase the driving forces of interfacial enzymatic reactions. Image, graphical abstract [ABSTRACT FROM AUTHOR]

High-solid anaerobic digestion (HAD) can directly treat dewatered sewage sludge (total solid content ≥15%) with superior volume efficiency. Sludge stabilization during HAD is expected to achieve by throughout organic degradation and conversion towards methane-rich biogas release and humic formation. Sewage sludge is the combination of microbial zoogleas and theirs adsorption of organic and inorganic matter, in which the extracellular polymeric substances (EPS) account 60–80% of total sludge organic matter, inevitably participating most extracellular metabolic pathways. The interactions between EPS transformation and genetically annotated metabolic pathways were found in this research. In brief, noticing the highly cross-linked structures in EPS with major active components of humic substances (HS) and protein (PN), as PN hydrolysis and decomposition in EPS were enhanced in the high-solid anaerobic condition, the exposure of aromatic groups and sites in HS were considerable. HS release was the main factor shifting the electron exchange capacity and activity, which aided in energy metabolism of sludge microorganisms involved in redox reactions, especially the methanogenesis, thus in turn facilitating the PN degradation; Then, the screened humic groups and active protein derives might act as the beneficial precursors to regenerate neo-humic structures, whose significant bridging effect and signal role on stimulating amino acid biosynthesis, member transport and metallic complexation could further contribute to proteolytic condensation and EPS reconstruction. Hence, the in-depth sludge stabilization mechanism during HAD process was established for developing enlightening strategies. Image 1 • Considerable HS initially released from EPS protein-binding structures disruption. • HS most determine the KEGG modules distribution (p < 0.05) during HAD. • HS exposure enhanced redox capacity of EPS and microbial energy metabolism. • HS release facilitated PN decomposition and prepared for aromatic regeneration. • PN condensation and EPS reconstruction mediated by HS achieve sludge stabilization. [ABSTRACT FROM AUTHOR]

Iron-based coagulants are dosed in enormous amounts and play an essential role in various segments of our urban water infrastructure. In order for the water industry to become circular, a closed-loop management strategy for iron needs to be developed. In this study, we have demonstrated for the first time that in-sewer dosed iron, either in the form of FeCl 3 or ferric-based drinking water sludge (Fe-DWS) as a means to combat sewer corrosion and odour, can be recovered in the form of vivianite in digested sludge in down-stream wastewater treatment plants. Importantly, about 92 ± 2% of the in-sewer dosed Fe was estimated to be bound in vivianite in digested sludge. A simple insertion of Neodymium magnet allowed to recover 11 ± 0.2% and 15.3 ± 0.08% of the vivianite formed in the digested sludge of the in-sewer dosed iron in the form of FeCl 3 and Fe-DWS, respectively. The purity of recovered vivianite ranged between 70 ± 5% and 49 ± 3% for in-sewer dosed FeCl 3 and Fe-DWS, respectively. Almost complete (i.e. 98 ± 0.3%) separation of Fe in the form of ferrihydrite was achieved from vivianite after alkaline washing. Subsequent batch experiments demonstrated that the recovered ferrihydrite can be directly reused for efficient sulfide control in sewers. At a ferrihydrite-Fe:S molar ratio of 1.2:1, sewage dissolved sulfide concentrations was reduced from 15 mgS/L to below 0.5 mgS/L within 1 h of reaction. Overall, the results obtained in our study flag a first step for utilities towards a closed-loop iron-based coagulant management approach. • Fe dosed to sewers as FeCl 3 was recovered from digested sludge as vivianite. • Ferric DWS dosed to sewers formed vivianite in down-stream anaerobic digestion. • Fe dosed to sewers as ferric DWS was recovered from digested sludge as vivianite. • Recovered Fe in the form of ferrihydrite effectively removed sulfide in sewage. • Practical feasibility of a closed-loop iron dosing strategy demonstrated. [ABSTRACT FROM AUTHOR]

The use of coagulants and flocculants in the water and wastewater industry is predicted to increase further in the coming years. Alum is the most widely used coagulant, however, the use of ferric chloride (FeCl 3) is gaining popularity. Drinking water production that uses FeCl 3 as coagulant produces waste sludge rich in iron. We hypothesised that the iron-rich drinking water sludge (DWS) can potentially be used in the urban wastewater system to reduce dissolved sulfide in sewer systems, aid phosphate removal in wastewater treatment and reduce hydrogen sulfide in the anaerobic digester biogas. This hypothesis was investigated using two laboratory-scale urban wastewater systems, one as an experimental system and the other as a control, each comprising sewer reactors, a sequencing batch reactor (SBR) for wastewater treatment, sludge thickeners and anaerobic digestion reactors. Both were fed with domestic wastewater. The experimental system received in-sewer DWS-dosing at 10 mgFe L−1 while the control had none. The sulfide concentration in the experimental sewer effluent decreased by 3.5 ± 0.2 mgS L−1 as compared with the control, while the phosphate concentration decreased by 3.6 ± 0.3 mgP L−1 after biological wastewater treatment in the experimental SBR. The dissolved sulfide concentration in the experimental anaerobic digester also decreased by 15.9 ± 0.9 mgS L−1 following the DWS-dosing to the sewer reactors. The DWS-doing also enhanced the settleability of the mixed liquor suspended sludge (MLSS) (SVI decreased from 193.2 ± 22.2 to 108.0 ± 7.7 ml g−1), and the dewaterability of the anaerobically digested sludge (the cake solids concentration increased from 15.7 ± 0.3% to 19.1 ± 1.8%). The introduction of DWS into the experimental system significantly increased the COD and TSS concentrations in the wastewater, and consequently the MLSS concentration in the SBR, however, this did not affect normal operation. The results demonstrated that iron-rich waste sludge from drinking water production can be used in the urban wastewater system achieving multiple benefits. Therefore, an integrated approach to urban water and wastewater management should be considered to maximise the benefits of iron use in the system. Image 1 • Fe-rich DWS dosed to sewers effectively decreases dissolved sulfides in sewers. • Fe-rich DWS dosed to sewers aids in P removal during wastewater treatment. • Fe-rich DWS dosed to sewers decreases sulfides in the AD sludge. • Fe-rich DWS dosed to sewers improves settleability and dewaterability of sludge. • Fe-rich DWS-dosing elevates MLSS in the WWTP. [ABSTRACT FROM AUTHOR]

The growing amount of sewage sludge from wastewater treatment plant is an emerging challenge in China. The efficient anaerobic digestion of sludge filtrate generated from hydrothermally pretreated sewage sludge can promote the disposal of sewage sludge. Herein, a pilot-scale anaerobic baffled reactor (ABR) assisted by symbionts of short chain fatty acid-oxidation syntrophs (SFAS) and exoelectrogens was developed to improve its stability and efficiency for filtrate treatment. The results demonstrated that the symbionts of exoelectrogens and SFAS, which were enriched by introduction of electrodes in the ABR system, promoted the degradation of butyric, propionic and acetic acids. Therefore, the COD removal efficiency increased from 74.1% to 86.6% and the methane content increased from 81.5% to 92.2% with methane production rising from 241 to 282 mL/g COD removed. Furthermore, the economic evaluation indicated that the energy consumption of electrodes was 0.600 kWh/m3 of sludge filtrate, the net energy profited from increased methane was 2.344 kWh/m3 of sludge filtrate. These results confirmed that the ABR system assisted by symbionts of SFAS and exoelectrogens was feasible for treatment of sludge filtrate in terms of both technical and economic level through pilot-scale verification. Image 1 • Anaerobic digestion of sludge filtrate assisted by SFAS and exoelectrogens was assessed. • The COD removal efficiency reached 86.6% with methane yield of 282 mL/g COD removed. • The operating cost of MECs was $ 0.084/m3 of filtrate. • Applying ABR system assisted by SFAS and exoelectrogens was feasible for sludge treatment. [ABSTRACT FROM AUTHOR]

Handling excess sludge produced by wastewater treatment is a common problem worldwide. Due to limited space available in landfills, as well as difficulties involved in using excess sludge in agriculture, there is a need for alternative disposal methods. Although anaerobic digestion (AD) is widely used in processing sludge, only partial energy recovery from methane and sludge volume reduction can be achieved, resulting in a substantial amount of sludge remaining, which needs to be disposed of. Direct incineration after sludge drying is one possible option, a practice that is already in place in some cities in China. A comparison between direct incineration and conventional AD (with or without pretreatment by thermal hydrolysis) has to be made with respect to the energy balance and investment & operational (I & O) costs. This comparison reveals direct incineration to have the lowest energy deficit and I & O costs. Therefore, it is expected that direct incineration without AD will become the preferred sustainable approach to handling sludge. Image 1 • Landfills and agricultural use of excess sludge is becoming increasingly constrained. • Alternative sludge disposal routes must be sought. • Anaerobic digestion (AD) is not a final solution for excess sludge. • Direct incineration without AD has the lowest energy deficit and I & O costs. • Focus on more efficient dewatering instead of AD is recommended. [ABSTRACT FROM AUTHOR]