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Neonicotinoid insecticides pose risks that need to be managed for conservation of aquatic ecosystems. In this study, we evaluated the associations between the estimated environmental concentrations of two neonicotinoid insecticides (imidacloprid and dinotefuran) and the total abundances of seven functional feeding groups (FFGs) of benthic invertebrate communities in rivers in urban rice-paddy areas in four Japanese regions. Regional datasets of benthic invertebrate communities and environmental variables available for Japan were analyzed. The associations between neonicotinoid exposure and benthic functional groups in each region were evaluated by applying a partial redundancy analysis to each regional dataset. We then examined whether there was an interregionally consistent pattern in the observed associations to ascertain the general applicability of the associations. In two of the four regions, the associations of the total abundances of the seven FFGs with neonicotinoid concentrations were significant, suggesting negative effects of imidacloprid and dinotefuran on river ecosystem functions in these two Japanese regions. Moreover, although the associations in the remaining two regions were not significant, the pattern of associations of the total abundances of six of the FFGs (shredders, filter feeders, collectors, grazers, predators, and scavengers) with imidacloprid concentrations seemed to be consistent among the four regions. This implies broad-scale negative effects of imidacloprid on river ecosystem functions in urban rice-paddy areas. We did not, however, find any interregionally consistent patterns in the associations with dinotefuran concentrations. This may be related to the multicollinearity with the imidacloprid concentrations and/or the low maximum dinotefuran concentration relative to the toxicity values of this neonicotinoid. Therefore, the association between dinotefuran and river ecosystem functions requires additional investigation. We believe that this type of hypotheses-generating research using country-wide biomonitoring and exposure databases can be a great aid in future ecological risk assessment studies. Unlabelled Image • Neonicotinoid (NN) risk assessment requires the use of observational field datasets. • Data from country-wide biomonitoring and exposure databases were analyzed. • Associations of functional groups of benthic invertebrates with NNs were evaluated. • Consistency in the associations among four Japanese regions were also examined. • Broad-scale effects of imidacloprid use on river ecosystem functions were found. [ABSTRACT FROM AUTHOR]

• Dragonfly abundance in Japan's agricultural landscapes has decreased. • We monitored the population density of the dragonfly Sympetrum frequens. • We estimated systemic insecticide use and analyzed its effect on the dragonfly. • The results suggest a negative effect of systemic insecticide use on population size. • Some agronomic factors need to be further examined as potential confounders. Since the mid-1990s, populations of the common Japanese dragonfly Sympetrum frequens in rice fields have declined severely. Application of systemic insecticides—especially fipronil—to nursery boxes of rice seedlings is suspected to be the main cause of the decline. However, until now there have been insufficient population data to test the causality. We conducted a dragonfly survey from 2009 to 2016 in four prefectures of Japan and compiled the data to enable the comparison of population growth rates along five main census routes over the years. We also estimated the use ratio of each insecticide applied to nursery boxes in rice fields (i.e., the area exposed to insecticide as a ratio of the total rice field area) by prefecture. We then statistically analyzed the effects of the insecticides on the dragonfly's population growth rates, taking into account the potential confounding factors based on current knowledge. There was a significant negative association between the annual increase in use ratio of the sum of systemic insecticides (e.g., fipronil, imidacloprid, and chlorantraniliprole) and the annual population growth rate of S. frequens. This association suggests that systemic insecticide use affected the decrease in population density of the dragonfly, although some agronomic factors need to be further examined as potential confounders. [ABSTRACT FROM AUTHOR]