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The non-scanning radiometer with short-wavelength (SW: 0.2–5.0 μm) and total-wavelength (TW: 0.2–50.0 μm) channels is the primary payload of the Integrated Earth–Moon Radiation Observation System (IEMROS), which is designed to provide comprehensive Earth radiation measurements and lunar calibrations at the L1 Lagrange point of the Earth–Moon system from a global perspective. This manuscript introduces a radiometer preflight calibration methodology, which involves background removal and is validated using accurate and traceable reference sources. Simulated Earth view tests are performed to evaluate repeatability, linearity, and gain coefficients over the operating range. Both channels demonstrate repeatability uncertainties better than 0.34%, indicating consistent and reliable measuring performance. Comparative polynomial regression analysis confirms significant linear response characteristics with two-channel nonlinearity less than 0.20%. Gain coefficients are efficiently determined using a two-point calibration approach. Uncertainty analysis reveals an absolute radiometric calibration accuracy of 0.97% for the SW channel and 0.92% for the TW channel, underscoring the non-scanning radiometer's capability to provide dependable global Earth radiation budget data crucial to environmental and climate studies. [ABSTRACT FROM AUTHOR]