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The purpose of this paper is to present a detailed zooarchaeological study conducted on the bone assemblage from the Laguna Cabeza de Buey 2 site, located on the margins of a small shallow lake in the center of the Pampa grasslands (province of Buenos Aires, Argentina). Taxonomic structure, taphonomic information, site formation processes, and subsistence strategies for different occupation events are described and discussed. At least three events of pre-Hispanic occupation (two in the Middle Holocene [6800 and 4150 14C years BP] and one in the Late Holocene) and one in Historical moments were identified. Human occupations are developed in relation to a shallow lake under a climate more arid during the Middle Holocene, which decreased up to the current environmental conditions; humid temperate. The camelid Lama guanicoe (guanaco) was always the most important economic resource during the three events of human occupation in the Holocene, followed by Ozotoceros bezoarticus (pampean deer) and, to a lesser extent, by three species of small armadillos ( Chaetophractus sp., Zaedyus pichiy , and Tolypeutes matacus ). In Historical times, modern domestic faunal remains ( Equus caballus and Bos taurus ) were exploited. A subsistence strategy focused on artiodactyls, mainly guanaco, allows suggesting certain specialization through time in the exploitation of animal resources by hunter-gatherer groups in the center of the Pampa grasslands, strategy that contrasts with what is recorded in other areas of the Pampean region. [ABSTRACT FROM AUTHOR]