1. MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production.
- Author
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Zhu, Xun, He, Zhenjian, Hu, Yiwen, Wen, Weitao, Lin, Cuiji, Yu, Jianchen, Pan, Jing, Li, Ran, Deng, Haijing, Liao, Shaowei, Yuan, Jie, Wu, Jueheng, Li, Jun, and Li, Mengfeng
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DENGUE hemorrhagic fever , *DENGUE viruses , *VIRAL replication , *VIRUS diseases , *IMMUNOREGULATION , *PUBLIC health - Abstract
MicroRNAs have been shown to contribute to a repertoire of host-pathogen interactions during viral infection. Our previous study demonstrated that microRNA-30e* (miR-30e*) directly targeted the IκBα 3′-UTR and disrupted the NF-κB/IκBα negative feedback loop, leading to hyperactivation of NF-κB. This current study investigated the possible role of miR-30e* in the regulation of innate immunity associated with dengue virus (DENV) infection. We found that DENV infection could induce miR-30e* expression in DENV-permissive cells, and such an overexpression of miR-30e* upregulated IFN-β and the downstream IFN-stimulated genes (ISGs) such as OAS1, MxA and IFITM1, and suppressed DENV replication. Furthermore, suppression of IκBα mediates the enhancing effect of miR-30e* on IFN-β-induced antiviral response. Collectively, our findings suggest a modulatory role of miR-30e* in DENV induced IFN-β signaling via the NF-κB-dependent pathway. Further investigation is needed to evaluate whether miR-30e* has an anti-DENV effect in vivo. Author Summary: Dengue is one of the most prevalent mosquito-borne viral diseases; though it is caused by the Dengue virus (DENV) in tropical/subtropical areas, it has shown tendency toward becoming a global public health concern, with estimated annual numbers of 50–100 million dengue infection cases and 500,000 people with severe disease who require hospitalization worldwide. Thus far no licensed vaccine or specific anti-DENV treatment for dengue is clinically available. Understanding the interaction of DENV with their human hosts is key to identifying potential therapeutic targets. In this work, we found that microRNA miR-30e* significantly suppressed DENV replication by promoting NF-κB-dependent IFN-β production. Our findings identifies miR-30e* as a possible restriction host factor for DENV infection, via positively modulating the antiviral innate immune response. Thus, this work broadens the understanding of the pivotal roles of miR-30e* in the interaction between DENV and the host. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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