1. Profiling of dynamic changes in non-volatile metabolites of shaken black tea during the manufacturing process using targeted and non-targeted metabolomics analysis.
- Author
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Xue, Jinjin, Liu, Panpan, Guo, Guiyi, Wang, Weiwei, Zhang, Jianyong, Wang, Wei, Le, Ting, Yin, Junfeng, Ni, Dejiang, and Jiang, Heyuan
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AMINO acid metabolism , *TIME-of-flight mass spectrometry , *MANUFACTURING processes , *METABOLOMICS , *CATECHIN , *PROCYANIDINS , *METABOLITES , *MULTIVARIATE analysis - Abstract
Shaken black tea (SBT) is a new type of black tea that combines the traditional black tea processing technology with the shaking process of oolong tea. In this study, non-targeted metabolomics analysis based on ultra-high-pressure liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) was applied to comprehensively analyze the non-volatile metabolites of SBT during the manufacturing process. Using multivariate statistical analysis, 93 compounds were screened as differential compounds. During the whole process of SBT, amino acids, dipeptides, theaflavins and lipids contents showed significant increases, while that of catechins and procyanidin C1 significantly decreased. Compared with fresh leaves, the content of proteinaceous amino acids (PAAs) in the dried black tea increased 1.61-fold, and total catechins decreased to 7.25%. The results of hierarchical clustering analysis (HCA) showed that fermentation is the key process that causes the variations of these metabolites, followed by drying and shaking. The biosynthesis metabolism of amino acids, flavonoids and linoleic acid are abundant, mainly due to the stress response of fresh tea leaves to dehydration and mechanical damage during shaking processing. It is beneficial to explore the quality formation during the production of SBT through comprehensive analysis of the changes in metabolites. • Fermentation led to significant changes in metabolites, followed by drying, shaking. • Shaking process promoted the accumulation of amino acids, dipeptides and theaflavins. • Remarkable increase of dipeptides is mainly in the fermentation and drying process. • Variation of taste compounds, amino acids, flavonoids, and dipeptides, were studied. • Metabolic pathway of key compounds like amino acids, flavonoids, lipids, were revealed. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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