Search

This study deals with the sea sand disruption method (SSDM) in sample preparation for chromatographic analysis of quercetin in different plants. Several SSDM variables were tested in relation to quercetin recovery: sample to sand mass ratio, type of dispersing liquid, volume of the solvent applied for analyte elution, and the time of grinding. The optimized procedure has been applied for the extraction of quercetin from the edible and inedible parts of different varieties of onion, apples, and tea. The SSDM results are comparable with those obtained by pressurized liquid extraction, regarded as one of the most effective sample preparation techniques. The presented results show that the hydrolysis of quercetin glycoside form in SSDM conditions does not occur or is negligible. Hence, the method is especially useful when the estimation of the real amount of aglycones in plants is required. [ABSTRACT FROM AUTHOR]

Four types of solvent extraction methods (ultrasound and microwave assisted extraction, pressurized liquid extraction, and extraction in the Soxhlet apparatus) for paclitaxel, cephalomannine, and 10-deacetylbaccatin, taxoids recovered from common yew twigs, were compared. By use of pressurised liquid extraction (PLE), the most effective extractant of taxoids was determined. HPLC was used for the analysis of the extracts. Comparison of the obtained results revealed differences in the extraction power of the applied methods. The greatest yields were obtained by multiple PLE, which can be recommended as the best sample preparation method for taxoids analysis in yew twigs. [ABSTRACT FROM AUTHOR]

The accelerated solvent extraction (ASE) procedure has been examined as a sample preparation method for the HPLC analysis of rutin and isoquercitrin in Sambucus nigra L. The experimental extraction parameters (solvent composition, temperature, operation pressure, and static extraction time) have been optimized in order to receive the best recovery of both analytes from the flowers, leaves, and berries of black elder. The ASE recoveries obtained under the optimal extraction conditions were compared with analogous ones obtained by means of the maceration technique. [ABSTRACT FROM AUTHOR]