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Objective To investigate the role of DNMT3A in IDH1 mutation induced acute myeloidleukemia genome methylation . Methods To study the effect of DNMT3A expression level on IDH1 induced genomic hypermethylation, human AML cell lines HL60 and THP-1 were transfected with IDH1，DNMT3A mutant expression plasmid. Results （1） The levels of 2-HG and 5-methylcytosine （5-mC） were increased in the primary AML cells with IDH1 mutation and the human AML cell lines HL60 or THP-1 transfected with IDH1 mutant expression plasmid was increased，while the level of DNMT3A mRNA and its protease activity also increased（P<0.05）.（2） Inhibiting the expression of DN MT3Ain HL60 or THP-1 cells transfected with IDH1 mutant expression plasmid could reduce the content of 5-mC（ P< 0.05），but the expression level of 2-HG was not affected （P = 0.989）.（3）Compared with HL60 cells transfected with IDH1 mutation （IDH1 R132H） expression plasmids alone， the expression plasmids transfected IDH1 mutation （IDH1 R132H） and DNMT3A mutation （DNMT3A R882H） decreased the content of 5-mC in cells（ P< 0.05）， but the expression of 2-HG wasn't affected （p=1.294） . Conclusion DNMT3A participates in the genomic hypermethylation induced by IDH1 mutation. [ABSTRACT FROM AUTHOR]