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Aquatic products with high moisture and protein content are susceptible to bacterial growth and spoilage. Searching for efficient and safe natural antibacterial agents to preserve aquatic products has been concerned widely. In this study, ε-poly-lysine-epigallocatechin gallate/sodium alginate-chitosan nanoparticles (ε-PL-EGCG/SA-CS NPs) were prepared using sodium alginate and chitosan as wall materials and ε-PL-EGCG as core material. The size of nanoparticles was about 200 nm and the encapsulation efficiency was 78.2%. Transmission electron microscopy (TEM) images confirmed the prepared spherical nanoparticles. Fourier transform infrared spectroscopy (FTIR) and multifunctional polycrystalline X-ray diffraction (XRD) spectra indicated that ε-PL-EGCG was encapsulated in the nanoparticles. Thermo-gravimetric analysis (TGA) illustrated that the thermal stability of encapsulated ε-PL-EGCG was improved more than that of bare ε-PL-EGCG. In addition, in vitro release assays showed that the ε-PL-EGCG was released continuously over 36 h. Bacteria inhibition results showed that the ε-PL-EGCG/SA-CS NPs significantly inhibited specific spoilage bacteria E3 that screened out of aquatic products, Escherichia coli and Staphylococcus aureus. In conclusion, ε-PL-EGCG/SA-CS NPs are an effective antibacterial means with wide application prospects in the field of aquatic products preservation. • Green antimicrobial core-shell nanoparticles (ε-PL-EGCG/SA-CS NPs) were prepared. • Combination of ε-PL and EGCG showed synergistic antibacterial effect. • The releasing of ε-PL-EGCG was prolonged after nano-capsulation with SA-CS. • The nanoparticles significantly inhibited spoilage bacteria isolated from aquatic products. [ABSTRACT FROM AUTHOR]

In this study, zein-loaded cinnamaldehyde (Cin@ZN) nanoparticles were incorporated into Chitosan (CS)/dialdehyde carboxymethyl cellulose (DCMC) matrix to fabricate the active food packaging materials possessing outstanding antioxidant and antibacterial properties. The research investigated how varying levels of Cin@ZN nanoparticles affected the morphology, microstructure, physicochemical properties of CS/DCMC composite films. The inclusion of Cin@ZN could significantly improve the mechanical strength, reduce the water vapor and oxygen permeability of CS/DCMC composite films and endow films with UV-light blocking properties. It's worth noting that the antibacterial and antioxidant capacities of CS/DCMC films had an astonishing enhancement with Cin@ZN blending, in which ABTS scavenging ratio of the composite films (100 mg) with different Cin@ZN contents reached >90 %. Furthermore, CS/DCMC/Cin@ZN 35 % composite film has the ability to efficiently protect strawberries from microbial damage and decelerate the spoilage rate of strawberries under ambient condition. Consequently, the CS/DCMC/Cin@ZN composite film can be applied as packaging material to extend the lifespan of fruits. [ABSTRACT FROM AUTHOR]

The mechanical mismatch between soft hydrated tissues and sutures has become a common negative impact on wound healing process. A novel method of coating multilayer polymer shells is thus reported to improve the mechanical performance of hydrogel sutures. It is suitable for tissue patching and shows advantages of convenient, efficient, and biosafety. Specifically, a precursor hydrogel (Cu@CMC) consisted of carboxymethyl chitosan and copper modified by carbon dots was used as the inner sheath, and then bonding the precursor hydrogel sheath with toughening polyethylene glycol network by anchoring sites composited from rigid chitosan shell integrated a whole structure. Subsequently, the whole system was soaked with EtOH, and rapid dehydration of EtOH was used to accelerate the entanglement process between the two coatings by constricting the molecular chains. Finally, an ideal suture (Cu-fiber) with both toughness and rigidness was obtained. The data showed that the tensile strength and biosafety of the hydrogel sutures prepared by the new strategy were significantly improved, and the skin, liver and vessel of rodents can be sutured without secondary damage. Moreover, it can inhibit inflammation response and promote the healing process of skin wound, indicating that the Cu-fiber will become a great candidate for tissue patching. [ABSTRACT FROM AUTHOR]

In this study, heparin-like polysaccharides were successfully produced by sulfation of carboxymethylcellulose sodium, then a fully biobased bilayer composed of sulfated carboxymethylcellulose sodium (SCMC) and chitosan (CS) was composited on the surface of Poly (L-lactic acid) (PLA) through layer-by-layer (LBL) assembly for the potential blood-contact application such as bioresorbable vascular scaffold. The preliminary structure and bioactivity of SCMC with different degree of sulfation were investigated, and the SCMC with best performance was selected. The surface chemical compositions, morphologies and wettability of SCMC/CS multilayer-modified PLA films were researched by X-ray photoelectron spectrometer, scanning electron microscopy and water contact angle meter. A series of anticoagulation tests of SCMC/CS multilayer-modified PLA films were performed. In term of (SCMC/CS) 15 multilayer-modified PLA film, the protein adsorption and plate adhesion decreased by 44.6 % and 71.5 %, respectively, the activated partial thromboplastin time prolonged by 11.9 times and thrombin time exceed 300 s, the contact activation and hemolysis rate significantly reduced compared with unmodified PLA film. Besides, this modified PLA films performed good cytocompatibility to L929 fibroblast cells, excellent anti-inflammatory and antibacterial abilities. In conclusion, the multifunctional SCMC/CS multilayer-modified PLA films with hemocompatibility, cytocompatibility, anti-inflammatory and antibacterial properties may have promising potential in future clinical applications. • Sulfated CMC showed good anticoagulation and anti-inflammatory abilities in vitro. • SCMC/CS multilayer-modified PLA film exhibited improved anticoagulation. • SCMC/CS multilayer-modified PLA film possessed good cytocompatibility. • SCMC/CS multilayer-modified PLA film showed excellent anti-inflammatory. • SCMC/CS multilayer-modified PLA film had excellent antibacterial property. [ABSTRACT FROM AUTHOR]

Herein, a novel magnetic cross-linked chitosan CS-BA@Fe 3 O 4 was rationally synthesized by cross-linked with epichlorohydrin and coated with Fe 3 O 4 to the acylated chitosan, which was prepared by the reaction of chitosan with benzenetricarboxylic anhydride. The as-obtained absorbent was characterized by FTIR, XRD, VSM, TGA, TEM, BET, SEM and EDS. The results showed that the maximum adsorption capacities of CR and CV were 471.46 ± 16.97 mg/g and 515.91 ± 25.12 mg/g at 318.15 K, respectively. The main adsorption mechanisms were H-bonding and electrostatic interaction. The kinetic data were in good agreement with the pseudo-second-order model and closed to adsorption equilibrium at 30 min. Thermodynamic studies showed that the adsorption on CS-BA@Fe 3 O 4 were spontaneous and endothermic. More importantly, the adsorbent exhibited excellent regeneration properties after 6 cycles and remarkable stability under harsh environments including strong acid, strong alkali, multi-salt and mixed dyes conditions. Therefore, abundant efforts revealed a broad application prospect of CS-BA@Fe 3 O 4 in water remediation. • A novel magnetic cross-linked functionalized chitosan adsorbent was synthesized. • Langmuir, Freundrich and Tempkin models fitted the data at three temperatures. • Maximum adsorption capacity for CR and CV were 471.5 and 515.9 mg/g, respectively. • At high concentration, the adsorption process were close to equilibrium at 30 min. • The adsorbent showed good selectivity, salt resistance and reusability. [ABSTRACT FROM AUTHOR]

Carbon dioxide (CO 2) assisted synthesis of water-soluble silver nanoparticle with a narrow particle size distribution is reported here based on the phase-inversion procedure. Bio-derived chitosan (CS) is used to stabilize the metal nanoparticles according to its abundant functional groups. Formic acid is employed as both a solvent (for the polymer) and a reductant for in-situ reducing the silver precursor along with the solvent evaporation. CO 2 is utilized to combine with the amino groups of CS, reducing the viscosity of chitosan/formic acid solution and limiting the formation of hydrogen bonds. This promotes the stabilization and reduction efficiency of silver nanoparticles. In particular, 100% of Ag metal nanoparticles with the size of 7.5 ± 2.3 nm is successfully synthesized with the assistance of CO 2. Interestingly, the synthesized CS/Ag nanocomposites are water-soluble owing to the formation of carbamate groups. This water-soluble silver nanoparticle presents an exceptional performance in the selective reduction of 4-nitrophenol, where the turnover frequency (TOF = 599 h−1) is even double with respect to the CO 2 free system. • CO 2 was used to reduce the viscosity of chitosan/formic acid solution, so as to limit the hydrogen bonds of the chitosan. • The carbon dioxide modified chitosan promotes the stabilization and reduction efficiency of silver nanoparticles. • The produced chitosan/silver nanocomposites are water soluble and present an exceptional catalytic performance. [ABSTRACT FROM AUTHOR]

Carboxymethyl chitosan and resistant starch exhibit good performance in diabetes regulation. We prepared carboxymethyl chitosan - resistant starch complex. Test the properties of composite resistant starch by using X-ray diffraction, water contact angle, infrared spectroscopy, and scanning electron microscopy, interactions with intestinal microbiota and mouse experiments were also conducted. The results indicated that the composite resistant starch had a good effect on promoting the proliferation of probiotics on Bifidobacterium and a significant inhibitory effect on Escherichia coli than resistant starch (P < 0.05). After administration, the water intake and weight of diabetic mice were significantly reduced. The blood glucose of diabetic mice was also reduced, and oral glucose tolerance showed that the glucose degradation rates of composite resistant starch were significantly improved compared to model mice. Cholesterol, triglycerides, high-density lipoprotein and low-density lipoprotein were significantly lower than those in the diabetes group (P < 0.05). The diversity of the gut microbiota was also proven. • Carboxymethyl chitosan can enhance the efficacy of resistant starch. • Carboxymethyl chitosan-resistant starch complex can adjust the gut flora better the resistant starch. • Carboxymethyl chitosan-resistant starch complex can improve the diabetes mice better the resistant starch. [ABSTRACT FROM AUTHOR]