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This study explored how co-oleogelator type, concentration, and water addition affect lipid digestion and β-carotene (βC) bioaccessibility in corn oil oleogels. Oleogels containing 0.1% βC, 20% glyceryl stearate (GS), with lecithin (L) or hydrogenated lecithin (HL) (at 0, 0.5, or 2.5%) and their water-filled counterparts (1% water) were examined. In vitro intestinal digestion revealed HL-oleogels experienced higher lipolysis due to their smaller crystal size enhancing surface area for lipase action, whereas L-oleogels presented lower digestibility, attributed to larger oil droplets and a minimized surface area. Water addition didn't significantly change lipid digestibility. βC bioaccessibility was inversely related to co-oleogelator concentration, with L-oleogels demonstrating the largest decrease, likely due to less free fatty acids released for micelle formation. However, water-filled oleogels enhanced βC bioaccessibility. These findings highlight that tailored microstructure in oleogels can control lipid digestion and βC bioaccessibility, paving the way for designing efficient delivery systems for targeted nutrient delivery. • Co-oleogelator Types Alter Oleogel Structure & Nutrient Access. • Lecithin (L) Reduces Oleogel In Vitro Lipid Digestibility. • Hydrogenated Lecithin (HL) Boosts Lipid Digestibility. • L and HL Decreases β-Carotene Bioaccessibility in Oleogels But Water Increases It. [ABSTRACT FROM AUTHOR]

• The effect of gamma irradiation on smoked bacon quality during storage was studied. • The artificial neural network was first used to predict irradiated smoked bacon quality. • Gamma irradiation can slow down the quality and sensory degradation of smoked bacon. • BP-ANN has great potential in predicting the gamma-irradiated bacon quality. This study investigated the effect of gamma irradiation on smoked bacon quality during storage and developed a multi-quality prediction model based on gamma irradiation. Gamma irradiation reduced moisture content and improved the microbial safety of smoked bacon. It also accelerated protein and lipid oxidation and altered free amino acids and fatty acids composition. It was effective in slowing down quality deterioration and sensory quality decline during storage. The backpropagation artificial neural network (BP-ANN) model was constructed by using physical and chemical indicators, irradiation dose, and storage time as input variables, and the total number of colonies and sensory scores as output layers. The transfer functions of the input-hidden layer and hidden-output layer were ReLu and Sigmoid, respectively. There were 13 neurons in the hidden layer. Results showed that BP-ANN based on physical and chemical indicators, irradiation dose, and storage time had great potential in predicting the multiple quality of smoked bacon. [ABSTRACT FROM AUTHOR]

[Display omitted] • Endogenous DHA in milk is more bioaccessible than exogenously added DHA. • Endogenous DHA in milk has higher digestive stability than exogenously added DHA. • The bound form of milk endogenous DHA facilitates its digestion and absorption. • Naturally enriched milk fat hydrolyzes easier than artificially enriched milk fat. This study aimed to compare the bioaccessibility of docosahexaenoic acid (DHA) in naturally and artificially enriched milk and investigate the potential mechanisms involved. The results indicated that the DHA in naturally enriched milk (NEM) had a higher bioaccessibility (76.9 %) and a lower digestive loss rate (18.1 %) compared to artificially enriched milk (ArEM). Moreover, NEM contained a higher proportion of DHA-containing glycerophospholipids and sn -2 DHA, with fewer long-chain fatty acids and more saturated fatty acids adjacent to DHA in the same lipid molecule. During simulated intestinal digestion, NEM had a higher free fatty acid release and lipid digestion rate than ArEM. These findings suggested that the bioaccessibility of endogenous DHA in milk was superior to that of externally added DHA due to its more easily digestible and absorbable chemical binding form and lower digestive loss rate. The easy digestibility of milk lipids in NEM also contributed to its high DHA bioaccessibility. [ABSTRACT FROM AUTHOR]

[Display omitted] • The evolution of volatiles in the lean and fat portions of bacon showed different patterns. • The aroma compounds were mainly provided by lean portion, and the optimal drying time was 24 h. • Eight key aroma compounds derived from different lipid fractions and fatty acids. • Linoleic acid was vital for the generation of key aroma compounds in non-smoked bacon. This study explored the evolution of key aroma compounds and their lipid precursors in the lean (LN) and fat (FT) portions of non-smoked bacon during hot air drying. The results showed that the LN portion contained most of the aroma compounds in the bacon (>88%). The volatile content of the FT portion increased as the drying time increased, whereas that of the LN portion reached a maximum within 24 h and then decreased. Based on the highest volatile contents (4889.48 ± 202.06 µg/kg) and sensory scores, 24 h was considered the optimal drying time. For key aroma compounds, hexanal and 2,3-octanedione were derived from free fatty acids and polar lipids. Notably, 1-octen-3-ol was generated only from polar lipids in the FT and LN portions. The 2-undecenal and (E, E)-2,4-decadienal were produced by the oxidation of neutral lipids in the FT portion. Dihydro-5-pentyl-2(3H)-furanone was derived from polar lipids in the LN portion. Altogether, these findings provide theoretical insights into improving the aroma of bacon by optimizing raw material selection and processing methods. [ABSTRACT FROM AUTHOR]

[Display omitted] • Toasted wheat bran protects RP in oil better from oxidation than native wheat bran. • A high bran-to-oil ratio lowers the stabilisation of vitamin A by native wheat bran. • High vitamin A and oil levels limit vitamin A stabilisation by toasted wheat bran. • Toasting reduces the capacity of wheat bran to protect lipids from oxidation. • Vitamin A acts as a pro-oxidant when mixed with oil and toasted wheat bran. Wheat bran stabilises vitamin A (retinyl palmitate, RP) in oil during storage, but the stabilisation mechanism remains unknown. We here studied the effect of the concentration of RP in oil (0.1–2%) and of RP-enriched oil in the system (5–50%) on the RP retention during accelerated storage of systems with native and toasted wheat bran. Generally, toasted bran showed better RP stabilisation than native bran. After four weeks of storage, up to 65% RP was retained in toasted bran systems, whereas the RP retention for native bran was below 10%. For native bran, a higher oil-to-bran ratio and, thus, a lower wheat lipase level resulted in better RP retention. For toasted bran, combined high oil and high RP concentrations resulted in the lowest RP retention. We, therefore, conclude that wheat bran protects RP and lipids from oxidation. This protection is reduced by the pro-oxidative effect of RP, lipid oxidation and lipase. [ABSTRACT FROM AUTHOR]

Highlights • A rapid lipid profiling platform was established using shotgun mass spectrometry. • An accurate quantification method for determination of TAGs and PLs was developed. • TAGs, PLs and FFAs in cold-pressed rapeseed oils were analyzed. • The PLs content raised up to 40 folds after microwave treatment of rapeseeds. Abstract Microwave pretreatment of rapeseeds prior to cold-pressing is a simple and desirable method for producing high quality oils. In this study, a rapid and sensitive lipid profiling platform employing an accurate quantification strategy was established based on direct infusion electrospray ionization tandem mass spectrometry. Using this developed platform, we further investigated the effect of microwave pretreatment of rapeseeds on the contents of important lipids such as triglycerides (TAGs), phospholipids (PLs), and free fatty acids (FFAs) in 15 different rapeseed oils. Our results demonstrated that no significant changes of total FAs and TAGs contents were observed after microwave pretreatment, while FFA contents increased and PLs contents significantly increased up to 40 folds. The potential mechanism of lipid changes was also discussed. The established lipidomics profiling platform can provide reliable lipids profiling data and help to better understand the potential mechanism of microwave pretreatment in oil processing. [ABSTRACT FROM AUTHOR]

• Lipidomics and volatilomics methods were employed. • 5 free fatty acids, 43 triglycerides, and 22 degradation products were identified. • EHT and EOL were the main biochemical behaviors during 0–28 days aging. • AOL was the main chemical change during 28–70 days aging. • A possible mechanism of brown rice quality deterioration during aging was proposed. Brown rice exhibits higher nutritional value and attracts more and more attentions; however, lipid alteration in brown rice during aging is poorly understood. In this study, lipidomics and volatilomics were employed to investigate free fatty acids, triglycerides, and volatile oxidative degradation products of lipids in brown rice during accelerated aging for 70 days. The results showed that the total free fatty acids in brown rice increased significantly (2.90–4.14 times) while triglycerides decreased remarkably at the initial stage of aging. Monounsaturated and polyunsaturated aldehydes, ketones, and acids increased obviously in brown rice during accelerated aging for 70 days. The screening of significantly different compounds indicated that the enzymatic hydrolysis of triglycerides (EHT) and enzymatic oxidation of lipids (EOL) were the main biochemical behaviors at the initial stage of aging (0–28 day) while automatic oxidation of lipids (AOL) was the primary chemical reaction for 28–70 days aging. [ABSTRACT FROM AUTHOR]

• The grape lipidome includes a wide range of species that goes far beyond traditional classes. • Approximately 300 lipid species covering 26 lipid classes were identified in grape samples. • Annotated lipids were complex lipids, mainly (lyso)glycerophospholipids and glycerolipids. • The most common fatty acyl chains were C16:0, C18:1, C18:2 and C18:3. • The lipidomic profile of grapes evolves during ripening. The aim of this paper is to provide a detailed characterisation of grape lipidome. To achieve this objective, it starts by describing a pipeline implemented in R software to allow the semi-automatic annotation of the detected lipid species. It also provides an extensive description of the different properties of each molecule (such as retention time dependencies, mass accuracy, adduct formation and fragmentation patterns), which allowed the annotations to be made more accurately. Most annotated lipids in the grape samples were (lyso)glycerophospholipids and glycerolipids, although a few free fatty acids, hydroxyceramides and sitosterol esters were also observed. The proposed pipeline also allowed the identification of a series of methylated glycerophosphates never previously observed in grapes. The current results highlight the importance of expanding chemical analyses beyond the classical lipid categories. [ABSTRACT FROM AUTHOR]

The micellar incorporation of carotenoids (lycopene, α - and β -carotene) and lipid digestion products (free fatty acids, FFAs, and monoacylglycerides, MAGs) during in vitro digestion of oil-in-water emulsions was investigated by a kinetic approach. A fractional conversion model could adequately describe the hydrolysis of triacylglycerides, formation of FFAs and MAGs, and micellar incorporation of carotenoids, FFAs and MAGs. The release of FFAs and MAGs from TAGs proceeded faster than their incorporation into micelles. Rate constants of carotenoid micellar incorporation were inversely proportional to their hydrophobicity and dependent on the isomeric configuration, being the incorporation of the cis faster than their all- trans isomers. Furthermore, a positive linear relation was found between the micellar incorporation of carotenoids and lipid digestion products. The isomeric form of carotenoids did not affect such relation. The present kinetic approach can be useful to gain mechanistic insight into carotenoid bioaccessibility as affected by various process- and product-related factors. [ABSTRACT FROM AUTHOR]

• The dynamic GI model was valuable to simulate and predict lipolysis rates; • Gastric lipase activity limited the extent of lipolysis during gastric digestion; • The dynamic protocol delivery of simulated gastric fluid regulated intragastric pH; • Free fatty acid profiles and concentrations related directly to lipase activities. Investigating the digestion of lipids is paramount for developing new lipid-based products. This work evaluated the gastrointestinal (GI) digestion of medium-chain fatty acids (MCFAs) rich lipids. The dynamic GI in vitro system was used to simulate gastric, duodenal, jejunal, and ileal GI tract portions. Results from the dynamic protocol were compared against static in vitro assays and GC analyses were conducted to assess the FA profile of FFA released during digestion. Caprylic and capric acids released during the gastric digestion of MCT oil varied from 61–63% and 36–38% of total esterified FA, respectively. Lauric acid was the most representative FFA released (31–54%) during the gastric digestion of coconut oil samples. It was observed that the gastric digestion phase plays a crucial role in the MCFA lipolysis and the lipase activity restricted the amount of free MCFA liberated during the GI digestion, resulting in incomplete lipids hydrolysis. [ABSTRACT FROM AUTHOR]

• The chilled aging time was closely related to the lipid content and species. • GP and FA were the main differential lipid classes during chilled aging. • 48 h of postmortem chilled aging was the turning point for lipid transformation. • Hydrolase activity and oxidation degree might be responsible for lipid changes. • Glycerophospholipid metabolism and thermogenesis are the major aging pathways. This present study aimed to investigate the effects of postmortem chilled aging on lipid and molecular transformation mechanisms in Mongolian sheep. UPLC-ESI-MS/MS was used to identify 812 lipids in Mongolian sheep within four days of chilled aging. Using multivariate analysis, 95 significant differential lipids were identified. The levels of phosphatidylcholine, phosphatidylinositol, and certain free fatty acids were observed to rise significantly, while other phospholipids and acylcarnitines showed a downward trend. In addition, levels of most of the lysophospholipids increased significantly in the early postmortem period; however, their levels did not alter significantly after 48 h. The Kyoto encyclopedia of genes and genomes pathway analysis revealed that these differential lipids are primarily involved in glycerophospholipid metabolism, linoleic acid metabolism, arachidonic acid metabolism, and thermogenesis. Our results have important implications in terms of understanding lipid transformation and changes in meat quality during postmortem chilled aging in Mongolian sheep. [ABSTRACT FROM AUTHOR]

[Display omitted] • Emulsifiers regulated the crystallization behavior of lipids. • The storage and photothermal stability of lutein were enhanced. • Rhamnolipid-stabilized lipid carriers exhibited better functional properties. • Emulsifiers affected the digestive characteristics of lutein loaded in lipid carriers. This study investigated the impacts of individual emulsifiers on the physicochemical stability, antioxidant ability, and in vitro digestion behavior of lutein-loaded nanostructured lipid carriers (NLCs). NLCs particles stabilized by ethyl lauroyl arginate, rhamnolipid, or tea saponin were fabricated by high-pressure microfluidization method. Differential scanning calorimetry and X-ray diffraction results confirmed the regulatory effect of emulsifiers on the crystallization behavior of NLCs. NLCs stabilized by rhamnolipid presented higher encapsulation efficiency (94.73%) for lutein than those stabilized by tea saponin (90.39%) or ethyl lauroyl arginate (88.86%). Meanwhile, the stability of embedded lutein during storage or photothermal treatments was greatly enhanced. Individual emulsifiers, together with lutein, endowed NLCs with excellent antioxidant capacity. During in vitro digestion, rhamnolipid-stabilized NLCs showed the slowest release of free fatty acids (50.87%) and provided an optimal sustained release for lutein with relatively high bioaccessibility (23.01%). [ABSTRACT FROM AUTHOR]

• Cur-OGs and Cur-NLC-OGs were successfully developed as a curcumin delivery system. • Application of NLC and biopolymer types affected the stability and lipid digestion of Cur-OGs. • Cur-NLC-OGs showed higher stability and FFAs release rate than those of Cur-OGs. • Carboxymethyl cellulose (CMC) based OGs retarded the lipid digestion. Curcumin-nanostructured lipid carrier-loaded oleogels (Cur-NLC-OGs) have been developed with biopolymer cryogels as an efficient delivery system to overcome the extremely low water solubility and instability of curcumin. The effect of NLC and biopolymer types on the encapsulation and release of curcumin from Cur-OGs was investigated. Alginate, carboxymethyl cellulose (CMC), and pectin solutions were firstly freeze dried to make biopolymer cryogels and they were mixed with Cur and Cur-NLC to obtain stable and self-standing Cur-OGs and Cur-NLC-OGs, respectively. As compared to Cur-OGs, Cur-NLC-OGs had higher encapsulation efficiency and showed slower release of curcumin under acidic condition. Although Cur-NLC affected the rapid release of free fatty acids, the Cur-NLC-OGs prepared with CMC cryogel was most efficient in delaying lipid digestion. Overall, NLC and CMC-based OGs could be effectively used to improve encapsulation efficiency and control lipolysis of lipid droplets. These results will be advantageous for the development of oleogels with desirable functionality. [ABSTRACT FROM AUTHOR]

[Display omitted] • Bulk compound chocolate products were developed as potential functional food. • Saturated fatty acids were less bioavailable using structured lipids. • The in vitro lipid digestion was reduced as well as its caloric impact. • Serum lipids were reduced in healthy C57BL/6J mice. The bioavailability impact of serum lipids in compound chocolate products based on structured lipids was studied. Compound chocolate products containing fat with and without structured lipids were digested in vitro under simulated gastrointestinal lipolysis conditions and were studied in vivo in healthy C57BL/6J mice. The in vitro digestion results show that products containing structured lipids, milk compound chocolate filling and white compound coating, significantly reduced the release rate of Free Fatty Acids (FFA) and improved the caloric reduction between 12.49% and 13.71% compared to products without structured lipids, suggesting that FFA were not absorbed. Animal feeding studies revealed no adverse effects on the compound products intake; in fact, these products reduced total cholesterol, LDL-c, VLDL-c and triacylglycerols. The present work shows the relevance of developing functional compound chocolate as providing a potential healthy initiative through the biological effect of the bioactive ingredients incorporated. [ABSTRACT FROM AUTHOR]

Abstract: Calanus finmarchicus is a marine zooplankton of interest for the aquaculture industry, as well as for nutraceuticals and the cosmetic industry. The chemical composition of C. finmarchicus rapidly changes postmortem due to autolytic processes; in particular phospholipids rapidly degrade to give free fatty acids. The aim of this study was to inactivate autolytic enzymes in C. finmarchicus by applying heat (72°C, 5–30min) through mixing with boiling, fresh water, and further to explore the effects of heat (70°C, 15min) combined with long time storage (−20°C, 12months) of treated and untreated material. Heat treatment (5min) inactivated all tested enzymes and maintained the initial amount of phospholipids, total lipids and crude protein. Storage of untreated material led to complete degradation of all phospholipids, whereas heat treatment resulted in a stable product containing the initial amount of phospholipids and astaxanthin. [Copyright &y& Elsevier]

Abstract: Vibrational spectroscopy (mid FTIR and FT-Raman) was used to monitor lipids extracted from hake fillets during frozen storage. Kramer shear resistance was used as a marker of texture changes and lipid damage was also investigated by following the development of conjugated dienes and free fatty acids by spectrophotometric methods. Results show that the intensity of the free fatty acid carboxylic ν(Ceasured by ATR-FTIR spectroscopy can be used for monitoring the development of lipid hydrolysis in hake lipids. Changes in the Raman ν(Ching region (1658cm−1 band), partially attributed to conjugated dienes development, were the only observed spectroscopic alterations related to lipid oxidation of hake lipids during frozen storage at −10°C. The high correlation of free fatty acids with instrumental texture and the disappearance of the ν as(PO2 −) band are consistent with membrane lipid hydrolysis being one of the factors directly related with toughening of lean fish flesh. [Copyright &y& Elsevier]

Abstract: Free fatty acids are strong prooxidants in both bulk and emulsified oils. Addition of oleic acid to an oil-in-water emulsions increased lipid hydroperoxide and hexanal formation at free fatty acid concentrations as low as 0.1% of the lipid. The prooxidant effect of free fatty acids was dependent on fatty acid type with lipid oxidation rates being in the order of linolenic

Abstract: The processing of fish roe leads to changes in its chemical composition, the extent of which depends on the techniques and additives employed. This study aimed to investigate the effects of ripening temperature and the use of sodium benzoate and citric acid on the quality of ripened cod roe, with respect to the contents of volatile base nitrogen (VBN), trimethylamine (TMA), biogenic amines (BA) and on the lipid composition. In comparison with fresh roes, ripened roes presented higher contents of VBN, TMA, BA and the proportion of free fatty acids regardless of the temperature and additives used during the ripening process. The greatest increases were observed in the samples ripened at 17°C without additives, in which histamine was detected at 8.8mg/100g. A low ripening temperature was the main factor responsible for minimising changes in the cod roe composition. The addition of sodium benzoate as a preservative or citric acid to decrease the pH value had a significant effect in maintaining the quality of the cod roes, mainly at high ripening temperature. [Copyright &y& Elsevier]

Abstract: The oxidative stability of lipids in processed quinoa was investigated in this study. Ground quinoa was subjected to accelerated aging for 30 days at 25, 35, 45, and 55°C. Three samples were removed from each temperature treatment every 3 days. Free fatty acids, conjugated diene hydroperoxides, and hexanal were used as indicators of lipid oxidation. Storage time and temperature had significant effects (p ⩽0.05) on all three parameters, while the interaction between storage time and temperature was not significant for conjugated diene hydroperoxides produced. The results from these tests suggest that quinoa lipids are stable for the period of time studied. With vitamin E as a naturally antioxidant occurring abundantly in quinoa, the potential for quinoa to be a new oilseed could be enhanced. This study provided some preliminary information on the oxidative stability of quinoa. [Copyright &y& Elsevier]

The influence of feeding Iberian pigs with and without α-tocopheryl acetate (α-TAc) on fatty acid composition of intramuscular lipids, throughout dry-cured ham maturation was investigated. Supranutritional levels of α-TAc (100 mg α-TAc/kg) increased α-tocopherol content in m. Semimembranosus in comparison to a basal diet containing 5 mg α-TAc/kg (Control: 6.38 vs. E-supplemented: 10.31 μg/g muscle dry matter). Fatty acid composition of lipids in the raw hams was similar whatever the level of vitamin E in the diet. Fatty acid composition of neutral lipids did not change much during ham maturation. In contrast, fatty acid content of polar lipids decreased considerably (∼90%) after 700 days of maturation. At the end of ripening (700 days), the contents of polyunsaturated fatty acids from neutral and polar lipids were significantly higher in hams from Iberian pigs fed on α-TAc-enriched diet than those fed with a basal diet. This result indicates that α-tocopherol-enriched diets maintain polyunsaturated fatty acids in neutral and polar lipid fractions of intramuscular fat in dry-cured hams from Iberian pigs. [Copyright &y& Elsevier]

• A mixture of free fatty acids inhibited lipid oxidation in ground, turkey thigh. • Oleic acid and linoleic acid could convert hemoglobin to hemichrome in spectra. • Increased number of double bonds in FFA facilitated hemichrome formation. • ESR spectra indicates the heme-Fe was in a low-spin state after Hb reacted with FFA. • Hemichrome formation due to added FFA diminished the lipid oxidation capacity of Hb. The pro-oxidant and anti-oxidant effects of free fatty acids (FFA) in meat remain ambiguous. To clarify the role of FFA in lipid oxidation of muscle food, the FFA was added into two systems (turkey mince and washed turkey muscle (WTM)) and lipid oxidation was investigated. A mixture of FFA inhibited lipid oxidation in both systems. The absorbance spectrum of oxyHb and metHb was determined in the presence of C18:1 and C18:2. Conversion to hemichrome spectra was observed and was particularly rapid when metHb was mixed with C18:2. C18:2 was also reacted with metHb followed by a chromatography step to remove unbound C18:2, and termed 'modified Hb'. Electron spin resonance (ESR) spectra of the modified Hb was indicative of hemichrome formation. The modified Hb did not promote lipid oxidation in washed turkey muscle during storage. This suggested that hemichrome formation due to added FFA diminished the lipid oxidation capacity of Hb. [ABSTRACT FROM AUTHOR]

• Understanding of the endogenous factors which effect lipid oxidation are needed. • Myoglobin promoted lipid oxidation in WCM but not WPM. • Two phospholipids (polyenoic indexes: 282, 24) showed similar oxidation rate in WPM. • Muscle microstructure plays critical roles in Mb-mediated lipid oxidation. • Accessibility of phospholipids to Mb was more important than its unsaturation. The roles of lipid oxidation substrates and muscle microstructure in lipid oxidation were investigated in two muscle models (cod and pig). Added myoglobin (Mb) promoted lipid oxidation in washed cod muscle (WCM) but not in washed pig muscle (WPM). The differing microstructure of WCM e.g. more exposed fat cells or membrane of muscle cells compared to the "denseness" or "wrapped" structure of WPM, may have contributed to the better ability of Mb to facilitate lipid oxidation in the WCM. Added phospholipids with polyenoic indexes of 282 and 24 activated Mb as an oxidant similarly in WPM while added neutral lipids and added free fatty acids had little effect. It is suggested that muscle microstructure and accessibility of Mb to phospholipids play critical roles in relation to Mb-mediated lipid oxidation while the degree of unsaturation in the phospholipids was less important. [ABSTRACT FROM AUTHOR]

• An updated in vitro digestive lipolysis model was adapted to preterm infant conditions. • Lower prelipolysis but similar digestive lipolysis were obtained in pasteurised vs raw human milk. • Intracellular lipid droplets were quantified by an adapted image analysis method. • Mean lipid droplet area in Caco-2 cells was smaller with pasteurised than with raw human milk. Donor human milk, pasteurised for safety reasons, is the first alternative for feeding preterm infants when mothers' own milk is unavailable. Breastmilk pasteurisation impact on lipid digestion and absorption was evaluated by a static in vitro digestion model for preterm infants coupled with intestinal absorption using Caco-2/TC7 cells. Lipid absorption was quantified by digital image analysis of lipid droplets, by measurement of basolateral triglyceride concentration and by analysing the expression of major genes involved. After in vitro digestion, lipolysis extent was 13% lower in pasteurised human milk (PHM) than in raw human milk (RHM). In Caco-2/TC7 cells, the number of lipid droplets was identical for both milk types, while the mean droplet area was 17% smaller with PHM. Altogether, pasteurisation decreased the pre-lipolysis of human milk. This initial difference in free fatty acid amount was only partially buffered by the subsequent processes of in vitro digestion and cellular lipid absorption. [ABSTRACT FROM AUTHOR]

• Glyceryl stearate concentration does not impact lipid digestibility. • Hydrogenated palm oil delays lipolysis kinetics of solid lipid nanoparticles. • β-Carotene concentration is determined by micelle fraction composition. • Monounsaturated fatty acids render micelles with higher solubilization capacity. Solid lipid nanoparticles (SLNs) are emulsion-based carriers of lipophilic bioactive compounds. However, their digestibility may be affected by the solid lipid phase composition. Hence, the aim of this work was to study the in vitro lipolysis kinetics as well as the relationship between the lipid digestion, micelle fraction composition and β-carotene bioaccessibility of SLNs with different solid lipids, being blends of medium chain triglyceride (MCT) oil, glyceryl stearate (GS) or hydrogenated palm oil (HPO) as compared to liquid lipid nanoparticles (LLNs) with pure MCT. SLNs formulated with GS were fully digested, similarly to LLNs. However, HPO-containing SLNs presented slower lipolysis kinetics during the intestinal phase at increasing HPO concentration. Despite this, HPO-SLNs showed higher β-carotene bioaccessibility, which was related to the higher amount of monounstaturated free fatty acids in the micelle fraction. Thus, this work provides valuable insight for designing delivery systems of bioactive compounds with optimal functionality. [ABSTRACT FROM AUTHOR]

• Optimum pH values of faba bean lipase and lipoxygenase (LOX) were characterised. • Faba bean had higher lipase activity than oat with triacylglycerols as substrates. • Specific profiles of volatiles from linoleic and α-linolenic acids for faba bean. • Lipid oxidation occurred during preparation of extracts and emulsions. • pH and addition of oil or fatty acids to extracts had a major effect on reactions. Faba bean can respond to the need for plant-based proteins for human consumption. The aim of this work was to study the role of lipid-modifying enzymes in faba bean in causing off-flavour compounds during processing. The faba bean exhibited high lipase and lipoxygenase (LOX) activities, with pH optima being 8.0 and 6.0, respectively. Faba bean LOX preferred free fatty acids (FFAs) over triacylglycerols as substrates, and together with other LOX pathway enzymes, it formed specific volatile products, as measured using headspace solid-phase microextraction–gas chromatography. During the preparation of the food models (i.e. the extracts and emulsions), enzymatic lipid oxidation occurred. The inclusion in the emulsions of rapeseed oil, especially of rapeseed oil FFAs, remarkably increased the amounts of volatile products. The largest quantities of products were formed in food models at pH 6.4, which is close to the pH optimum of LOX. Further studies on lipase in food models are needed. [ABSTRACT FROM AUTHOR]