1. Exposure to Traffic-Related Air Pollution and Serum Inflammatory Cytokines in Children.
- Author
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Gruzieva, Olena, Merid, Simon Kebede, Gref, Anna, Gajulapuri, Ashwini, Lemonnier, Nathanaël, Ballereau, Stéphane, Gigante, Bruna, Kere, Juha, Auffray, Charles, Melén, Erik, and Pershagen, Göran
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NITROGEN oxide analysis , *RNA analysis , *AIR pollution , *ASTHMA , *AUTOMOBILES , *BIOMARKERS , *CONFIDENCE intervals , *CYTOKINES , *GENE expression , *INFLAMMATION , *INTERLEUKINS , *PROBABILITY theory , *QUESTIONNAIRES , *REGRESSION analysis , *RESEARCH funding , *PARTICULATE matter , *DATA analysis software , *DESCRIPTIVE statistics , *MANN Whitney U Test , *CHILDREN - Abstract
BACKGROUND: Long-term exposure to ambient air pollution can lead to adverse health effects in children; however, underlying biological mechanisms are not fully understood. OBJECTIVES: We evaluated the effect of air pollution exposure during different time periods on mRNA expression as well as circulating levels of inflammatory cytokines in children. METHODS: We measured a panel of 10 inflammatory markers in peripheral blood samples from 670 8-y-old children in the Barn/Child, Allergy, Milieu, Stockholm, Epidemiology (BAMSE) birth cohort. Outdoor concentrations of nitrogen dioxide (NO2) and particulate matter (PM) with aerodynamic diameter <10 μm (PM10) from road traffic were estimated for residential, daycare, and school addresses using dispersion modeling. Time-weighted average exposures during infancy and at biosampling were linked to serum cytokine levels using linear regression analysis. Furthermore, gene expression data from 16-year-olds in BAMSE (n=238) were used to evaluate links between air pollution exposure and expression of genes coding for the studied inflammatory markers. RESULTS: A 10 μg=m³ increase of NO2 exposure during infancy was associated with a 13.6% (95% confidence interval (CI): 0.8; 28.1%) increase in interleukin-6 (IL-6) levels, as well as with a 27.8% (95% CI: 4.6, 56.2%) increase in IL-10 levels, the latter limited to children with asthma. However, no clear associations were observed for current exposure. Results were similar using PM10, which showed a high correlation with NO2. The functional analysis identified several differentially expressed genes in response to air pollution exposure during infancy, including IL10, IL13, and TNF. CONCLUSION: Our results indicate alterations in systemic inflammatory markers in 8-y-old children in relation to early-life exposure to traffic-related air pollution. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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