1. Spectroscopic studies on the interaction of γ-cyclodextrin–daunorubicin inclusion complex with herring sperm DNA
- Author
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Xu, Dongling, Wang, Xingming, and Ding, Lisheng
- Subjects
- *
CYCLODEXTRINS , *DAUNOMYCIN , *SPERMATOZOA , *DNA , *FLUORESCENCE spectroscopy , *ENTHALPY , *ABSORPTION , *X-ray diffraction , *VISCOSITY - Abstract
Abstract: The interaction of daunorubicin (DNR) with herring sperm DNA in cyclomaltooctaose (γ-cyclodextrin, abbrev. γ-CD) supramolecular system was studied by UV–vis absorption spectroscopy, fluorescence spectroscopy and viscosity method. On the condition of physiological pH, the results indicate that DNR prefers to form the 1:1 inclusion complex with γ-CD. These results are further supported by infrared spectrometry (IR), and X-ray diffraction (XRD). An inclusion constant (K) for the formation of γ-CD–DNR inclusion complex has been evaluated to be 9412.39Lmol−1 from the absorbance changes. It is observed that the interaction mode between γ-CD–DNR and DNA are a mixed binding, which contains partial intercalation and groove binding. The binding ratio of γ-CD–DNR with DNA is n γ-CD–DNR:n DNA =10:1, binding constant is K Θ 298.15K =2.44×105 Lmol−1, enthalpy and entropy work as driven force in this action. [Copyright &y& Elsevier]
- Published
- 2011
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