1. Fungal Secretomes of Aspergillus terreus Repertoires Cultivated on Native and acid/alkali Treated Paddy Straw for Cellulase and Xylanase Production.
- Author
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Kaur, Gurkanwal, Taggar, Monica Sachdeva, Kalia, Anu, Krishania, Meena, and Singh, Alla
- Subjects
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ASPERGILLUS terreus , *CELLULASE , *XYLANASES , *STRAW , *LIGNIN structure , *ALKALIES , *FILTER paper , *HEMICELLULOSE , *AMYLOLYSIS - Abstract
The potential of native as well as acid/alkali pre-treated paddy straw was investigated as inducer substrate for Aspergillus terreus mediated cellulase and xylanase production. The cellulose, hemicellulose and lignin contents of native straw were found to be 25.94%, 17.64% and 15.82%, respectively. However, in the acid/alkali treated straw, cellulose content substantially increased to 48.09%, whereas hemicellulose and lignin contents were reported to be particularly low (11.72%, 5.08%, respectively). The FTIR spectra of native and pre-treated paddy straw further supported the variation in their chemical constituents. A. terreus repertoires were cultivated on native and treated paddy straw under solid state fermentation (SSF) and liquid fermentation (LF) conditions. The LF cultures showed higher cellulase and xylanase activities (Filter Paper cellulase: 45.33 U/L, Carboxymethyl cellulase: 104.75 U/L, β-glucosidase 80.44 U/L, Avicelase: 22.72 U/L and Xylanase: 623.69 U/L) than the respective SSF cultures. Significantly high activities of exoglucanases and endoglucanases were obtained when native straw was used as the growth substrate. The scanning electron micrographs of native and treated straw exhibited disintegration after being subjected to biological treatments, confirming the hyphal adhered growth and production of hydrolysing secretomes by the fungus. Cultivation of A. terreus on native paddy straw in LF culture system resulted in better enzyme production, and holds promise for further optimization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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