7 results on '"Kawaoka, Yoshihiro"'
Search Results
2. A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus.
- Author
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Fonseca, Wendy, Ozawa, Makoto, Hatta, Masato, Orozco, Esther, Martínez, Máximo, and Kawaoka, Yoshihiro
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RECOMBINANT viruses , *INFLUENZA viruses , *VIRAL vaccines , *CHIMERIC proteins , *RESPIRATORY syncytial virus infections , *VIRAL replication , *RNA polymerases - Abstract
Infections with influenza and respiratory syncytial virus (RSV) rank high among the most common human respiratory diseases worldwide. Previously, we developed a replication-incompetent influenza virus by replacing the coding sequence of the PB2 gene, which encodes one of the viral RNA polymerase subunits, with that of a reporter gene. Here, we generated a PB2-knockout recombinant influenza virus expressing the F protein of RSV (PB2-RSVF virus) and tested its potential as a bivalent vaccine. In mice intranasally immunized with the PB2-RSVF virus, we detected high levels of antibodies against influenza virus, but not RSV. PB2-RSVF virus-immunized mice were protected from a lethal challenge with influenza virus but experienced severe body weight loss when challenged with RSV, indicating that PB2-RSVF vaccination enhanced RSV-associated disease. These results highlight one of the difficulties of developing an effective bivalent vaccine against influenza virus and RSV infections. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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3. Attenuation of an influenza A virus due to alteration of its hemagglutinin-neuraminidase functional balance in mice.
- Author
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Gen, Fumihiro, Yamada, Shinya, Kato, Kentaro, Akashi, Hiroomi, Kawaoka, Yoshihiro, and Horimoto, Taisuke
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INFLUENZA A virus , *HEMAGGLUTININ , *NEURAMINIDASE , *ANIMAL models in research , *MEMBRANE glycoproteins , *SIALIC acids - Abstract
Influenza A viruses possess two surface glycoproteins, hemagglutinin (HA), which binds to sialic-acid-containing receptors, and neuraminidase (NA), which removes sialic acid from host cells. It is well established that the HA-NA functional balance regulates the efficiency of virus replication. Here, we selected a plaque variant of the WSN (H1N1) strain that grew better than the wild-type virus in NA-expressing MDCK cell culture. A reverse genetics study revealed that the single mutation HA E190K, which occurs infrequently in naturally isolated H1N1 viruses, was responsible for the phenotype of this variant. Receptor assays indicated that this mutation did not affect the receptor specificity of HA but enhanced its receptor-binding affinity, resulting in altered HA-NA functional balance relative to that of the wild-type virus. We also found that this variant replicated in nasal turbinates at an equivalent level but in lungs at a lower level compared with wild-type virus, demonstrating its attenuation in mice. Together, our data demonstrated the importance of the HA-NA functional balance for influenza virus replication in an in vivo biological setting. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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4. Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations.
- Author
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Kuhn, Jens, Becker, Stephan, Ebihara, Hideki, Geisbert, Thomas, Johnson, Karl, Kawaoka, Yoshihiro, Lipkin, W., Negredo, Ana, Netesov, Sergey, Nichol, Stuart, Palacios, Gustavo, Peters, Clarence, Tenorio, Antonio, Volchkov, Viktor, and Jahrling, Peter
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CLASSIFICATION of viruses , *TAXONOMY , *PHYLOGENY , *PLETHORA (Pathology) , *BLOOD circulation disorders , *MORPHOLOGY , *VIROLOGISTS , *RNA - Abstract
The taxonomy of the family Filoviridae (marburgviruses and ebolaviruses) has changed several times since the discovery of its members, resulting in a plethora of species and virus names and abbreviations. The current taxonomy has only been partially accepted by most laboratory virologists. Confusion likely arose for several reasons: species names that consist of several words or which (should) contain diacritical marks, the current orthographic identity of species and virus names, and the similar pronunciation of several virus abbreviations in the absence of guidance for the correct use of vernacular names. To rectify this problem, we suggest (1) to retain the current species names Reston ebolavirus, Sudan ebolavirus, and Zaire ebolavirus, but to replace the name Cote d'Ivoire ebolavirus [sic] with Taï Forest ebolavirus and Lake Victoria marburgvirus with Marburg marburgvirus; (2) to revert the virus names of the type marburgviruses and ebolaviruses to those used for decades in the field (Marburg virus instead of Lake Victoria marburgvirus and Ebola virus instead of Zaire ebolavirus); (3) to introduce names for the remaining viruses reminiscent of jargon used by laboratory virologists but nevertheless different from species names (Reston virus, Sudan virus, Taï Forest virus), and (4) to introduce distinct abbreviations for the individual viruses (RESTV for Reston virus, SUDV for Sudan virus, and TAFV for Taï Forest virus), while retaining that for Marburg virus (MARV) and reintroducing that used over decades for Ebola virus (EBOV). Paying tribute to developments in the field, we propose (a) to create a new ebolavirus species ( Bundibugyo ebolavirus) for one member virus (Bundibugyo virus, BDBV); (b) to assign a second virus to the species Marburg marburgvirus (Ravn virus, RAVV) for better reflection of now available high-resolution phylogeny; and (c) to create a new tentative genus ( Cuevavirus) with one tentative species ( Lloviu cuevavirus) for the recently discovered Lloviu virus (LLOV). Furthermore, we explain the etymological derivation of individual names, their pronunciation, and their correct use, and we elaborate on demarcation criteria for each taxon and virus. [ABSTRACT FROM AUTHOR]
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- 2010
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5. Induction of TNF-α in human macrophages by avian and human influenza viruses.
- Author
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Monteerarat, Yuwarat, Sakabe, Saori, Ngamurulert, Somying, Srichatraphimuk, Sirawat, Jiamtom, Wasana, Chaichuen, Kridsada, Thitithanyanont, Arunee, Permpikul, Parichart, Songserm, Taweesak, Puthavathana, Pilaipan, Nidom, Chairul A., Le Quynh Mai, Iwatsuki-Horimoto, Kiyoko, Kawaoka, Yoshihiro, and Auewarakul, Prasert
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INFLUENZA A virus, H5N1 subtype , *TUMOR necrosis factors , *INFLUENZA viruses , *MACROPHAGES , *GENETICS - Abstract
The highly pathogenic avian influenza virus H5N1 is known to induce high level of tumor necrosis factor α (TNF-α) from primary macrophages. However, it is still unclear whether current H5N1 strains also induce high TNF-α production, as most of the data were derived from extinct clade 0 H5N1 strain. Here, we show that current clade 1 and 2 H5N1 strains induce variable levels of TNF-α that are not necessarily higher than those induced by seasonal influenza viruses. The result suggests that hyper-induction of TNF-α in human macrophages is not always associated with a highly pathogenic phenotype. We further tested the contribution of the NS gene segment from H5N1 isolates to TNF-α induction by using reverse genetics. While NS conferred some variation in TNF-α induction when incorporated into an H1N1 virus genetic background, it did not affect TNF-α induction in an H5N1 virus genetic background, suggesting that other viral genes are involved. [ABSTRACT FROM AUTHOR]
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- 2010
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6. A comparison of the pathogenicity of avian and swine H5N1 influenza viruses in Indonesia.
- Author
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Takano, Ryo, Nidom, Chairul A., Kiso, Maki, Muramoto, Yukiko, Yamada, Shinya, Shinya, Kyoko, Sakai-Tagawa, Yuko, and Kawaoka, Yoshihiro
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PATHOGENICITY of enteroviruses , *INFLUENZA , *RESPIRATORY infections , *COMMUNICABLE diseases - Abstract
Highly pathogenic avian influenza H5N1 viruses are circulating in many countries. We recently discovered that these viruses have been transmitted to pigs on multiple occasions in Indonesia. To investigate whether avian H5N1 influenza viruses adapted to mammals through their introduction into pigs, we examined the growth of avian and swine isolates in cell culture and compared their pathogenicity in mice. We found that swine isolates were less virulent to mice than avian isolates, suggesting that the viruses became attenuated during their replication in pigs. Continuous surveillance of H5N1 viruses among pigs is clearly warranted. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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7. Pathogenicity of Chinese H5N1 highly pathogenic avian influenza viruses in pigeons.
- Author
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Beibei Jia, Jianzhong Shi, Yanbing Li, Shinya, Kyoko, Muramoto, Yukiko, Xianying Zeng, Guobin Tian, Kawaoka, Yoshihiro, and Hualan Chen
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AVIAN influenza , *PIGEONS , *VIRUS diseases in poultry , *PATHOGENIC microorganisms , *RESPIRATORY infections , *DISEASES - Abstract
It has long been thought that pigeons are resistant against H5 highly pathogenic avian influenza (HPAI) viruses. Recently, however, highly pathogenic H5N1 avian influenza viruses have demonstrated distinct biological properties that may be capable of causing disease in pigeons. To examine the susceptibility of domestic pigeons to recent H5N1 viruses, we inoculated pigeons using H5N1 viruses isolated in China from 2002 to 2004. Within 21 days following inoculation, all pigeons had survived and fully recovered from temporary clinical signs. However, seroconversion assays demonstrated that several viruses did in fact establish infection in pigeons and caused a certain amount of viral shedding in the oropharynx and cloaca. There was not, however, a definitive relationship between viral shedding and viral origin. Viruses were also inconsistently isolated from various organs of pigeons in infected groups. Pathological examination revealed that the infection had started as respiratory inflammation and caused the most severe lesions in the brain in later stages. These results indicate that pigeons are susceptible to the more recent Asian H5N1 HPAI and could be a source of infection to other animals, including humans. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
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