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[Objective] Heat shock factors (HSFs) are the major transcription factors of eukaryotic heat shock responses. This study aims to investigate the adversity stress tolerance functions of Arabidopsis heat shock factor AtHsfA1a, which has important significance for in-depth understanding of adversity stress tolerance mechanisms of plants and further utilization of heat shock factor genes. [Method] Genomic DNA of Arabidopsis was extracted with CTAB method and purified to obtain Arabidopsis DNA samples for in vitro site-specific recombination cloning (Gateway cloning) to construct plant expression vector of heat shock factor AtHsfA1a. Firstly, donor vector pDONR 201/AtHsfA1a was constructed based on attB and attP site-specific recombination method (BP reaction), to identify E. coli transformants harboring correct sequence of AtHsfA1a by sequencing; secondly, plant expression vector pB7WG2/AtHsfA1a overexpressing Arabidopsis heat shock factor AtHsfAla was constructed based on attL and attH site-specific recombination method (LR reaction), to screen E. coli transformants harboring target plas-mid. [Result] Plant expression vector of Arabidopsis heat shock factor gene AtHsfAla was constructed successfully. [Conclusion] This study not only provided experimental materials for acquiring transgenic plants overexpressing heat shock transcription factor AtHsfA1a, but also laid the foundation for further investigation of the diversity of adversity stress tolerance functions regulated by HSFs. [ABSTRACT FROM AUTHOR]

[Objective] This study aimed to investigate the influence of high temperature on the expression of heat shock transcription factor AtHsfAla in different genotypes of Arabidopsis. [Method] Arabidopsis plants overexpressing heat shock transcription factor AtHsfAla were used as experimental materials and treated under high temperature at 39 °C for 1 min and 5 min; total RNA of AtHsfAla was extracted, and the reverse transcription and amplification were conducted using RT-PCR technology, the amplification products were detected by electrophoresis. [Result] The expression levels of AtHsfAla in Arabidopsis plants overexpressing heat shock transcription factor AtHsfAl a at high temperature and room temperature were higher than wild-type Arabidopsis; the expression levels of AtHsfAla in both wild-type Arabidopsis and transgenic Arabidopsis plants overexpressing heat shock transcription factor AtHsfAl a at high temperature of 39 °C were higher than that at room temperature of 25 °C, but the expression levels of AtHsfAla in wild-type Arabidopsis and transgenic Arabidopsis plants overexpressing heat shock transcription factor AtHsfAla varied little after high temperature treatment at 39 °C for 1 min or 5 min. [Conclusion] The expression of AtHsfAla is induced rapidly by high temperature, thus regulating the expression of early adversity-resistant genes. This study will lay the foundation demonstrating the mechanism of Arabidopsis heat shock transcription factor AtHsfA1a. [ABSTRACT FROM AUTHOR]