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CRISPRized Horticultural Crops: Genome Modified Plants and Microbes in Food and Agriculture summarizes applications of CRISPR/Cas systems and its advanced variants e.g., CRISPR/Cpf1, base editing and prime editing, for precise editing of horticultural crops. The book discusses vector transformations methods, epi-genome, deep learning, synthetic biology, and precision breeding for improving yield and quality related attributes in horticultural crops. With coverage of the relevant technologies and their applications, the book also includes bioinformatics and large-scale databases and their potential application in fruits, vegetables and ornamental plants and sections on regulatory concerns related to CRISPR edited crops. Horticultural crops, including fruit, vegetable and ornamental plants are an important component of agriculture production systems and play an important role in sustaining human life. - Reviews CRISPR for editing horticultural crops - Discusses vector transformation methods, epigenome, deep learning, synthetic biology and precision breeding - Includes bioinformatics and large-scale databases - Contributes engineering approaches for crop improvement programs

Biotechnology and Crop ImprovementThe green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in biotechnology, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenic, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. Biotechnology and Crop Improvement: Tissue Culture and Transgenic Approaches focuses on important field crops to highlight germplasm enhancement for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits and improved tolerance to increasing temperature and introduces significant recent achievements in crop improvement using methods such as micropropagation, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture, and compressing the breeding cycle for accelerated breeding and early release of crop varieties. Plant biotechnology has now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenic now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research, and industry and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. It describes the recent biotechnological advancement and developments in plant tissue culture and transgenic. Plant tissue culture techniques such as such as micropropagation, regeneration, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture are discussed for genetic improvement of crop plant. Transgenic techniques are discussed for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits, and improved tolerance to increasing temperature.Key Features Shows the importance of plant tissue culture and transgenic technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant tissue culture and transgenic technology This book is devoted to topics with references at both graduate and postgraduate levels. The book traces the roots of plant biotechnology from the basic sciences to current applications in the biological and agricultural sciences, industry, and medicine. The processes and methods used to genetically engineer plants for agricultural, environmental, and industrial purposes along with bioethical and biosafety issues of the technology are vividly described in the book.

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book is the first attempt for in-depth compilation of current knowledge on cisgenic crops and their potential prospects as a sustainable substitute for the controversial genetically modified crops. Innovative methodologies for the development of cisgenic crops for disease resistance, improved nutritional contents, suitability for organic farming, survival under climate change, and their role in conservation of plant genetic resources have been highlighted. Combined with molecular markers and genome editing, an advanced approach for crop improvement is reported. The book has 14 chapters authored by globally leading experts on the subject. This book is useful to the students, teachers, researchers and policy planners working across the disciplines of classical plant breeding up to the recent genetically modified and genome edited crops.

Genetic transformation is a key technology, in which genes are transferred from one organism to another in order to improve agronomic traits and ultimately help humans. However, there is apprehension in some quarters that genetically modified crops may disturb the ecosystem. A number of non-governmental organizations continue to protest against GM crops and foods, despite the fact that many organisms are genetically modified naturally in the course of evolution. In this context, there is a need to educate the public about the importance of GM crops in terms of food and nutritional security. This book provides an overview of various crop plants where genetic transformation has been successfully implemented to improve their agronomically useful traits. It includes information on the gene(s) transferred, the method of gene transfer and the beneficial effects of these gene transfers and agronomic improvements compared to the wild plants. Further, it discusses the commercial prospects of these GM crops as well as the associated challenges. Given its scope, this book is a valuable resource for agricultural and horticultural scientists/experts wanting to explain to the public, politicians and non-governmental organizations the details of GM crops and how they can improve crops and the lives of farmers.

Policy Issues in Genetically Modified Crops: A Global Perspective contains both theoretical and empirical evidence of a broad range of aspects of GM crop policies throughout the world. Emphasizing world agriculture production and ethics of GM crops, the book balances insights into the various discussions around the use of GM crops including soil health, effects on animals, environmental sustainability impact, and ethical issues. The book presents aspects of GM crop policies and prevailing controversies throughout the world, in 5 sections containing 23 chapters. Beginning with the discussion of the policies related to GM crops, the book dives deep into issues related to food insecurity, agricultural sustainability, food safety, and environmental risks. Section 5 also captures the recent advances in agricultural biotechnology encompassing research trends, the nano-biotech approach to plant genetic engineering, and other transformation techniques in crop development. The contributors of the book represent different backgrounds, providing a holistic overview of diverse approaches and perspectives. Policy Issues in Genetically Modified Crops: A Global Perspective is a valuable resource for researchers in agricultural policy and economics, agricultural biotechnology, soil science, genetic engineering, ethics, environmental management, sustainable development, and NGOs. - Discusses ethics, varieties, research trends, success, and challenges of genetic modification - Addresses both crop production and potential health impacts - Includes extensive theoretical research and studies

This book will be of immense helpful to the students of plant biotechnology, Agricultural sciences, Microbiology of both undergraduate and postgraduate levels in universities, colleges, and Research institutes. Besides the book will be quite supportive researchers who work in the field of plant biotechnology and agricultural sciences. In this book, the main focus will be on advanced genome editing approaches for the production of GM crops besides their socioeconomic, ethical and risk-biosafety assessments. Nanotechnology is the new emerging and fascinating field of science finds its application in almost all the major research areas and its uses in agriculture and food sectors are incipient.The books seems to be first in summarizing the two way interactive approach in the field of plant biotechnology and setting of a new arena in shaping the new bio techniques towards the sustainable cause.

Genetic Engineering of Horticultural Crops provides key insights into commercialized crops, their improved productivity, disease and pest resistance, and enhanced nutritional or medicinal benefits. It includes insights into key technologies, such as marker traits identification and genetic traits transfer for increased productivity, examining the latest transgenic advances in a variety of crops and providing foundational information that can be applied to new areas of study. As modern biotechnology has helped to increase crop productivity by introducing novel gene(s) with high quality disease resistance and increased drought tolerance, this is an ideal resource for researchers and industry professionals. - Provides examples of current technologies and methodologies, addressing abiotic and biotic stresses, pest resistance and yield improvement - Presents protocols on plant genetic engineering in a variety of wide-use crops - Includes biosafety rule regulation of genetically modified crops in the USA and third world countries

This book analyzes the impacts of current and possible future GM crop applications and shows that these technologies can contribute substantially to sustainable agricultural development and food security.

This book provides a comprehensive and in-depth discussion on the development of herbicide resistance during the past 50 years, emphasizing the biochemical pathways of herbicide resistance in weeds. It discusses the principles of plant genetics, different methods of genetic engineering, makingof transgenic plants, various transgenic crops conferred

This book presents a holistic view of the complex and dynamic responses of plants to nanoparticles, the signal transduction mechanisms involved, and the regulation of gene expression. Further, it addresses the phytosynthesis of nanoparticles, the role of nanoparticles in the antioxidant systems of plants and agriculture, the beneficial and harmful effects of nanoparticles on plants, and the application of nanoparticles and nanotubes to mass spectrometry, aiming ultimately at an analysis of the metabolomics of plants. The growing numbers of inventions in the field of nanotechnology are producing novel applications in the fields of biotechnology and agriculture. Nanoparticles have received much attention because of the unique physico-chemical properties of these compounds. In the life sciences, nanoparticles are used as “smart” delivery systems, prompting the Nobel Prize winner P. Ehrlich to refer to these compounds as “magic bullets.” Nanoparticles also play an important role in agriculture as compound fertilizers and nano-pesticides, acting as chemical delivery agents that target molecules to specific cellular organelles in plants. The influence of nanoparticles on plant growth and development, however, remains to be investigated. Lastly, this book reveals the research gaps that must be bridged in the years to come in order to achieve larger goals concerning the applications of nanotechnology in the plants sciences.In the 21st century, nanotechnology has become a rapidly emerging branch of science. In the world of physical sciences, nanotechnological tools have been exploited for a broad range of applications. In recent years, nanoparticles have also proven useful in several branches of the life sciences. In particular, nanotechnology has been employed in drug delivery and related applications in medicine.

Plant molecular biology came to the fore in the early 1980s and there has been tremendous growth in the subject since then. The study of plant genes and genomes and the development of techniques for the incorporation of novel or modified genes into plants eventually led to the commercialisation of genetically modified (GM) crops in the mid-1990s. This was seen as the start of a biotechnological revolution in plant breeding. However, plant biotechnology has become one of the hottest debates of the age and, in Europe at least, one of the greatest challenges that plant scientists have ever faced.This book covers the history and development of the science and techniques that underpin plant biotechnology. It describes the GM crops that are or have been grown commercially around the world, including failures as well as successes, and the new varieties that are being developed. The safety record of GM crops is reviewed together with the legislation that has been adopted to cover their use. The book also deals with the concerns of consumers, the GM crop debate and the prospects for the technology. In the second edition, sections on current GM crops and future developments in plant biotechnology have been greatly expanded, while those on techniques, legislation and the GM crop debate have also been updated.The book is a concise, comprehensive and readable study that is accessible to a general readership with a scientific background but also provides useful information for the specialist.

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Plants whose DNA is modified using genetic engineering techniques are known as transgenic plants. In most cases the aim is to introduce a new trait to the plant which does not occur naturally in this species. Examples include resistance to certain pests, diseases or environmental conditions, or the production of a certain nutrient or pharmaceutical agent. This new book gathers and presents current research on transgenic plants including an examination of the release, persistence and transport of transgenic plant DNA in saturated and unsaturated mediums, such as soils and sediments; high-efficient plant cultivation with artificial light sources; the impact of three antibiotics on seed germination, hydroponic growth and nutritional qualities of vegetables; growth responses of Bt and non-Bt cottons to soil phosphorous, copper and cadmium levels; site-specific recombination systems for genome manipulation in transgenic plants; and amplification of small interfering RNAs in transgenic plants.

Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.

Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 2 with 13 chapters contributed by 41 eminent scientists from nine countries deliberates on the utilization of transgenic crops for resistance to herbicides, biotic stress and abiotic stress, manipulation of developmental traits, production of biofuel, biopharmaceuticals and algal bioproducts, amelioration of ecology and environment and fostering functional genomics as well as on regulations and steps for commercialization, patent and IPR issues, and compliance to concerns and compulsions of utilizing transgenic plants.

Genetic engineering is a powerful tool for crop improvement. The status of crop biotechnology before 2001 was reviewed in Transgenic Crops I-III, but recent advances in plant cell and molecular biology have prompted the need for new volumes. Following Transgenic Crops IV (2007) on cereals, vegetables, root crops, herbs, and spices, and Transgenic Crops V (2007) on fruits, trees, and beverage crops, this volume, Transgenic Crops VI, contains the following sections: Oils and Fibers, Medicinal Crops, Ornamental Crops, Forages and Grains, Regulatory and Intellectual Property of Genetically Manipulated Plants. It is an invaluable reference for plant breeders, researchers and graduate students in the fields of plant biotechnology, agronomy, horticulture, forestry, genetics, and both plant cell and molecular biology.

Genetic engineering is a powerful tool for crop improvement. The status of crop biotechnology before 2001 was reviewed in Transgenic Crops I-III, but recent advances in plant cell and molecular biology have prompted the need for new volumes.Following Transgenic Crops IV (2007) dealing with cereals, vegetables, root crops, herbs and spices, this volume, Transgenic Crops V, is devoted to fruit, trees and beverage crops. Fruit crops targeted include banana, citrus, mango, papaya, pineapple, watermelon, avocado, grape, melon, apple, Prunus spp., strawberry, and kiwifruit, while trees include walnut, rubber, eucalyptus, tropical tree legumes, Casuarina glauca and conifers. The section on beverage crops reports studies on coffee, cacao, tea, and sugarcane.This volume is an invaluable reference for plant breeders, researchers and graduate students in the fields of plant biotechnology, agronomy, horticulture, forestry, genetics, and both plant cell and molecular biology.

Genetic engineering is a powerful tool for crop improvement. Crop biotechnology before 2001 was reviewed in Transgenic Crops I-III, but recent advances in plant cell and molecular biology have prompted the need for new volumes. Transgenic Crops IV deals with cereals, vegetables, root crops, herbs and spices. Section I is an introductory chapter on the impact of plant biotechnology in agriculture. Section II focuses on cereals (rice, wheat, maize, rye, pearl millet, barley, oats), while Section III is directed to vegetable crops (tomato, cucumber, eggplant, lettuce, chickpea, common beans and cowpeas, carrot, radish). Root crops (potato, cassava, sweet potato, sugar beet) are included in Section IV, with herbs and spices (sweet and hot peppers, onion, garlic and related species, mint) in Section V. This volume is an invaluable reference for plant breeders, researchers and graduate students in the fields of plant biotechnology, agronomy, horticulture, genetics and both plant cell and molecular biology.

En dix ans, les plantes transgéniques ont atteint plus de 90 millions d'hectares dans le monde. En revanche, leur développement est bloqué en France et en Europe. Sujet de société, elles sont au cœur de débats polémiques et l'objet de prises de positions politiques. Le but de cet ouvrage est de rassembler dans un même document les informations pour mieux comprendre les objets du débat. Qu'est-ce que la transgénèse, qu'apporte-t-elle ou que peut-elle apporter? Quels sont les risques éventuels pour l'environnement et la santé? Comment sont-ils gérés ou peuvent-ils être gérés? Quelles sont les raisons de l'opposition au développement des plantes transgéniques? Cet ouvrage s'adresse à un public assez large s'intéressant aux questions posées par le développement des plantes transgéniques.

The book is written for policy experts, life scientists, government and business leaders, biotechnology writers and social activists. Few decision-makers realize the unprecedented degree to which transgenic technology is now possible for forests on a commercial scale. Only a handful of the 550 living conifer species is used for commodity value and even fewer species are being developed for transgenic plantations. Transgenic field trials started within the last decade but no transgenic pine plantations exist in 2005. But emergence of transgenic forest trees is still so recent that dialogue about the pros and cons is confined to the scientific community. And dialogue must move out into the public domain. The goal of this volume is to provide content for public deliberations about the genetic composition of future forests. Its Section I is composed of provocative and opposing views on the question of transgenic conifer plantations. Sections II and III follow with research advances on relevant conifer genomics and ecology research, respectively. Section IV forecasts rates of technology adoption for different case studies. Finally, Section V compares the status of regulatory oversight of transgenic forest trees between Canada and the United States.

At a time when much of humanity is already but one failed harvest removed from starvation, we cannot afford to ignore any potential danger to food security, especially when that danger poses a threat to rice, the staff of life for so much of the world. Crop Ferality and Volunteerism brings together research pioneers from various disciplines

This book focuses on critical issues in the development of transgenic crops, their problems and prospects, both in developed and developing countries. It deals with genetically-modified herbicide tolerant crops and transgenic crops that resist diseases.

Gain state-of-the-art knowledge of new research and developments in transgenic technology! Genetically Modified Crops: Their Development, Uses, and Risks provides groundbreaking information on the integration of foreign DNA into the nucleus of a plant cell to produce a positive transformation. This volume details methods of gene delivery, laboratory tools and techniques to increase success rates, and the benefits, risks, and limitations of these methods. Authors at the forefront of this developing technology provide a comprehensive overview of transgenic crops and vital research on specific plant genera that have undergone transgenic transformation. Agricultural biotechnology has become a national and necessary mainstay of farming and food production, and this book is an important scientific tool to keep you informed of the latest protocols of genetic transformation. This book also outlines the goals that scientists are striving to reach, such as targeted gene expression where the gene only expresses itself at a certain time in the plant's life cycle, but disappears before human consumption. One of the greatest concerns is maintaining the welfare of the consumer, and in this volume the authors repeatedly discuss their findings in terms of safety for human consumption. With Genetically Modified Crops: Their Development, Uses, and Risks, you'll explore: the history of crop transformation and the techniques most commonly used for gene delivery, including biolistic bombardment and Agrobacterium-mediated transformation various methods of determining successful gene transfer in putative transgenic plants, such as blotting, functional assaying, and progeny testing the utilization of recombinase-directed plant transformation to improve faithful and consistent gene delivery and transference the successful reproduction of an insecticidal protein from chicken eggs in transgenic corn—and its benefits to society the current status of risk assessment and examples of incidents that have raised the level of concern about genetically modified plants outside the lab This book also contains several chapters about current methods of transformation involving specific crops such as: cotton wheat alfalfa sorghum rice and more! Genetically Modified Crops: Their Development, Uses, and Risks is an indispensable guidebook for agronomists, plant and molecular geneticists, and students in agronomy, genetics, entomology, horticulture, and plant pathology. This manual is also useful to concerned consumers who wish to know the latest scientific findings on genetically modified crops. Complete with references, figures, and photographs, this book is a must-read to keep up to date with science and technology.

Genetically engineered plant products line the shelves of our grocery stores but we don't know which ones they are because no label identifies them. Should we be concerned? Biotech companies claim that engineered corn and canola are safe, but are they telling the truth? Should we, like the Europeans, be engaging in violent protests against biotechnology? In High Tech Harvest, Paul Lurquin answers these questions and more, believing that the public has a right to know and understand how its food is manipulated at the most basic level, that of the DNA itself. With the goal to inform, and a mission to reinforce the importance of the scientific method, Paul Lurquin writes a comprehensive and user-friendly description of the scientific origins, the development, and the applications of genetically modified plants throughout the world today.

The world's food production is undergoing a rapid and revolutionary transformation, but little is known about it and less is being done to question the wisdom of it. Within a very few years, much of what we eat will have been genetically engineered, without proper consideration of the issues of public health, consumer choice and ecological stability. Against the Grain argues that the consequences of this huge experiment could be catastrophic, and at the very least have been underestimated or ignored by the industries exploiting the new technologies. The authors have unearthed government and in.

Use of genetically engineered plants for food production has raised many questions about food safety. Scientists, environmentalists, and government regulators have debated safety issues since the advent of genetic engineering.

Biotechnology offers tremendous potential for improving crop production, animal agriculture, and bioprocessing. It can provide scientists with new ways to develop higher-yielding and more nutritious crop varieties, to improve resistance to disease, or to reduce the need for inputs of fertilizers and other expensive agricultural chemicals. This book explores the United States'ability to solve important agricultural problems, effectively use funds and institutional structures to support biotechnology research for agriculture, train researchers in new scientific areas, efficiently transfer technology, and regulate and test recombinant DNA organisms in the field.