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This well-accessible textbook constitutes a practical guide to metabolomics approaches and applications in an integrative manner. Illustrated by numerous real-life examples and case studies the book teaches how to set up and carry out metabolomics experiments, from sample collection to storage techniques, and guides the reader through the potentials and challenges of metabolite analysis. In addition, this textbook shows how to obtain insights into molecular mechanisms deployed in different physiological contexts (e.g., exercise, healthy ageing) as well as in different (metabolic) diseases, be it Diabetes, Cancer, Alzheimer´s Disease, Asthma or others. The book brings together knowledge from biochemical, biological, and medical aspects, and further equips the reader with know-how from sample collection and handling to data processing, analysis and interpretation. This textbook is a must-have for advanced undergraduates and graduate students interested in applied biochemistry and metabolomics, as well as for instructors who teach courses in biomedicine, physiology or biochemistry.