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The discovery of adenoviruses naturally induced a new interest in viruses of the human upper respiratory tract since previously unknown viruses infecting this portion of the human body had not been identified in 20 years, and their unique characteristics stimulated investigations into the biochemical events essential for replication of animal viruses. Indeed, the field of molecular virology has evolved during the period since their dis­ covery, and adenoviruses have played a major role in this development. The exciting discoveries made with adenoviruses have had such a pro­ found effect on knowledge in basic virology, molecular biology, viral ge­ netics, human and animal infections, and cell transformation that this seemed a propitious time to have some of the major contributors review this field. This volume pays tribute to the late Wallace Rowe, Robert Huebner, and Maurice Hilleman whose initial discoveries of adenoviruses have tremendously enriched virology. Harold S. Ginsberg vii Contents Chapter 1 An Overview 1 Harold S. Ginsberg Chapter 2 The Architecture of Adenoviruses M. V. Nermut I. Introduction.................................... 5 II. Chemical and Physical Properties.................... 6 III. Virus Capsid: Composition and Organization........... 7 A. Hexon...................................... 10 B. Penton..................................... 12 C. Other Virus Polypeptides Associated with the Capsid 13 D. Organization of the Capsid...................... 14 IV. Virus Core..................................... 15 A. Evidence for the Core Shell...................... 17 B. Organization of the DNA-Protein Complex (Nucleoc- sid)........................................ 18 C. Tentative Model of the Adenovirus Nucleocapsid.... 22 V. Model ofthe Adenovirion.......................... 29 32 References...........................................

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.