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Flowers and other ornamental plants are used for all occasions to meet consumers demands preferably novel flowers traits, e.g., fragrance, flower color and shape, early flowering, less water consumption, long shelf-life. The worldwide floricultural industry is worth over 50 billion Euros and can serve as a ‘food security', socio-economic impact, and generate employment. Ornamental industry is regarded as one of the fastest growing farm industries. This industry is sustained through novelty, thus there is increasing demand on plant breeders in both public and private sectors to fulfil consumer's needs. Biotechnological approaches such as genetic transformation, genomics, nanotechnology, and gene editing are well suited for designing custom-made novel traits of flowers benefiting both ornamental and cosmetic industry. Moreover, micropropagation is well exploited commercially for large-scale plant production along with vertical and digital farming, and artificial intelligence especially by the floriculture industry. This book focuses on advances in breeding strategies of diverse range of ornamental plants. It consists of 2 parts, Part I Flowering annuals and Part II Cut flowers. Each chapter, contributed by eminent authors, is devoted to an individual ornamental species or a group of related species. It provides an in depth understanding of modern breeding strategies including traditional methods and biotechnological approaches. Topics covered in each chapter, in relation to the subject species, include current cultivation practices and challenges, germplasm biodiversity and conservation, traditional breeding, molecular breeding, tissue culture applications, genetic engineering and gene editing, mutation breeding, hybridization, and future research directions. Major concepts are illustrated with color photos.

This book explains the advancements of plant biotechnology and advanced molecular biology and explores the details of influential tools that complement conventional breeding and accelerate the development of plants resilient to adverse agroclimatic conditions and biofortified plants. Plant biotechnology from the basic sciences to current applications, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors, and scaling up in bioreactors, have been included in these chapters to improve the production of secondary metabolites from different medicinal plants. It also highlights important factors often overlooked by methodologies used to develop plants'tolerance against biotic and abiotic stresses and in developing special foods, bio-chemicals, and pharmaceuticals. This book is valuable for researchers or students working on biosciences. It is also an updated and advanced reference material for the agriculture and pharmaceutical industries.

This reference is a comprehensive review of genome editing in bacteria. The multi-part book meticulously consolidates research findings and insights on the applications of bacteria across several industries, including food processing and pharmaceutical development. The book covers four overarching themes for readers: a historical perspective of genome editing, genome editing in probiotics, applications of genome editing in agricultural microbiology and genetic engineering in environmental microbiology. The editors have also compiled chapters that provide an in-depth analysis of gene regulation and metabolic engineering through genome editing tools for specific bacteria.Key topics in part 2:- Targeting pathogenic microbes for plants and animals using CRISPR-CAS- Genome editing microbes to improve crop yield plant growth for sustainable agriculture- Applications of genome editing for bioremediation- Microbial genome editing for environmental bioprocessing- Genetic engineering for methanotrophs- Genome engineering in Cyanobacteria- Genome editing in StreptomycesGenome Editing in Bacteria is a definitive reference for scholars, researchers and industry professionals navigating the forefront of bacterial genomics.

This book is a collection of information about applying CRISPR-Cas systems for genome editing in plants. The main focus of this book is to address the recent advances and future prospects of CRISPR-Cas technology in crops.Genome editing technology is important because it can be used to improve plant traits. The earlier genome-engineering tools, zinc finger nucleases (ZFNs), and TAL effector nucleases (TALENs) are complicated to design and not flexible. The novel genome editor, CRISPR-Cas systems, has advantages over ZFNs and TALENs. The advantages are simple and easy to design precision in targeting and efficiency. Due to its precision and simplicity, the CRISPR-Cas technology has rapidly become the most popular genome-editing platform in life-science fields. CRISPR-Cas technology has been used widely for human gene therapy to treat diseases and for plant breeding programs for crop improvement.This book is of interest and useful to genome-editing professionals, plant breeders, horticulturists, field-level extension workers, nurserymen, planters, ecologists, and valuable source of reference to the relevant researchers.

Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging'CRISPR/Cas9'technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach. This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases. - Explains basic mechanism and implementation of CRISPR/Cas9 technology in a wide range of plants. - Provides practical guidance on the applications of CRISPR/Cas9 in different scientific disciplines of plant science. - Discusses the risks and challenges of genome engineering.

This book entitled, The Pearl Millet Genome, is the first comprehensive compilation of deliberations on history, domestication, genetic and genomic resources, traditional breeding, genetic diversity, molecular maps and mapping of important biotic stress as well as nutritional quality traits, whole genome sequencing and comparative genomics, functional genomics, genetic transformation. The economic, nutritional, and health importance of the pearl millet is also discussed. It also presents the input use efficiency and wide adaptation of the crop. Altogether, the book will contain about 200 pages over 10 chapters authored by globally reputed experts on the relevant field in this crop. This book will be useful to the students, teachers, and scientists in the academia and relevant private companies interested in genetics, pathology, molecular genetics and breeding, genetic engineering, structural and functional genomics, and nutritional quality aspects of the crop. This book will also be also useful to seed and pharmaceutical industries.

With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese, and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing.While molecular biotechnology offers unlimited opportunities for improving human health and well-being, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to dampen the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the Introduction provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse, and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors.Key features Presents state-of-the-art reviews on DNA, RNA and protein technologies and their biotechnological applications Discusses key strategies, effective thinking, and experimental execution for scientific research and development Fills the gap left by detailed-ridden laboratory manuals and insight-lacking standard textbooks Includes expert contributions from international scientists at the forefront of molecular biotechnology research and development Written by international scientists at the forefront of molecular biotechnology research and development, chapters in this volume cover the histories, principles, and applications of individual techniques/technologies, and constitute stand-alone, yet interlinked lectures that strive to educate as well as to entertain. Besides providing an informative textbook for tertiary students in molecular biotechnology and related fields, this volume serves as an indispensable roadmap for novice scientists in their efforts to acquire innovative skills and establish solid track records in molecular biotechnology, and offers a contemporary reference for scholars, educators, and policymakers wishing to keep in touch with recent developments in molecular biotechnology.

Over the years, the interventions of genomics tools have paved the way for molecular breeding to meet the challenges of food security and climate resilience. Advances in plant molecular breeding encompassing approaches of genomics, molecular markers, genetic transformation and genome editing have revolutionized crop improvement. Successful application of these tools has led to improvement of a wide range of traits of agronomic relevance. This book provides a comprehensive coverage of successful applications of molecular approaches that can be integrated within plant breeding programs aimed at improvement of crop plants. The book covers all relevant areas of molecular breeding applications in plants, with many examples drawn from the advanced genomics and molecular breeding research. Chapters present a critical appraisal of the current literature in the respective fields of molecular breeding written by expert authors. Each chapter provides in-depth discussion of the subject supported with high-quality color illustrations relevant data and future research perspective and, a comprehensive list of pertinent references.

This is the first comprehensive monograph dedicated to using Nicotiana species for tobacco genetic improvement. Unlike typical chapters constrained by size and scope, this book offers a detailed species-by-species analysis, with 27 tables providing relevant information. It marks a shift from focusing on specific topics to giving due attention to individual species. The review reflects on a century of exploiting Nicotiana species for usable germplasm and serves as an exhaustive guide to global literature on the subject, citing nearly 1000 literature items.The review delves into the involvement of Nicotiana species in interspecific hybrids with cultivated tobacco, covering sexual and asexual methods, including grafting. It addresses challenges in hybridization, such as cross incompatibility, maternal phenotypes, interspecific incongruity, lethality, and sterility, providing methods to overcome these barriers. Records on disease resistance for each Nicotianae species are compiled, and mechanisms of introgression, barriers, and limitations are discussed. The book lists Nicotiana species as successful donors of cytoplasmic genetic factors leading to cytoplasmic male sterility (CMS) and discusses negative effects of alien cytoplasm, providing a list of agronomically acceptable CMS sources. Controversial issues, including the identity of genetic accessions, are explored. The book concludes with an updated list of all reported interspecific combinations for Nicotiana.Primarily aimed at scholars and students interested in the genus for genetic diversity, this book serves as a historical reference guide to the realized and potential uses of Nicotiana species in tobacco improvement.

This new 2-volume set explores new research and perspectives in genetic engineering, which enables the precise control of the genetic composition and gene expression of organism. This powerful technology can be used for environmental sustainability, food and nutritional security, medicinal advancement, and more. Genetic Engineering aims to provide a deep understanding of the many aspects of this emerging technology and its diverse applications. Genetic Engineering, Volume 1: Principles, Mechanism, and Expression covers genetic engineering concepts, molecular tools, and technologies utilized in the manipulation, amplification, and introgression of DNA. The volume explains the concepts of genetic engineering, enzymes of genetic engineering, and tools used in genetic engineering. It provides an introduction of recombinant DNA into host cells and discusses the linking of desired gene with DNA vector/gene cloning vector, polymerase chain reactions, the concept and nature of genes, blotting techniques, chromosome jumping, electrophoresis, genetically engineered microorganisms, and molecular markers and their applications. Genetic Engineering, Volume 2: Applications, Bioethics, and Biosafety expresses the various appreciation and challenges of genetic engineering and issues related to bioethics and biosafety. Chapters cover the legal issues of genetic engineering, including intellectual property rights (IPR) and protection (IPP) and the patenting of living organisms, copyrights, trade secrets, and trademarks. The volume considers the safety and benefits of genetic engineering in human welfare, such as in genetically engineered Bt and Bt cotton, along with the biohazards of recombinant DNA technology. Chapters explain genetically modified organisms and microorganisms, genetic engineering of horticultural crops, genetic engineering in the agricultural sciences, and more. This 2-volume book will be a valuable asset to upper-level students in cell biology as well as to faculty and researchers involved in genetics, molecular genetics, biochemistry, biotechnology, botany, zoology and agriculture sciences.

'An indispensable source for researchers, teachers, and graduate and postgraduate students interested in mutation breeding and genetic engineering. It introduces readers to contemporary knowledge and state-of-the-art technologies in the field of mutation breeding, including fundamental mechanisms and applications.... It will provide new directions, and avenues for enhancement of food security and food quality by using the latest techniques for the'mutation as breeding'approach.'- From Prof. Jameel M. Al-Khayri, King Faisal University, Saudi Arabia This comprehensive three-volume set book aims to help combat the challenge of providing enough food for the world by the use of advanced genetic processes to improve crop production, both in quantity and quality. Volume 1: Mutagenesis and Crop Improvement discusses mutagenesis, cytotoxicity, and crop improvement, covering the processes, mutagenic effectiveness, and mechanisms. The volume emphasizes the improvement of agronomic characteristics by manipulating the genotype of plant species, resulting in increased productivity. Volume 2: Revolutionizing Plant Biology covers the use of mutagenesis and biotechnology to explore the variability of mutant genes for crop improvement. The chapters deal with in-vitro mutagenesis to exploit the somaclonal variations induced in cell culture and highlight the importance of in-vitro mutagenesis in inducing salt resistance, heat resistance, and drought resistance. Volume 3: Mechanisms for Genetic Manipulation of Plants and Plant Mutants reviews the genetic engineering techniques used to mutate genes and to incorporate them into different plant species of cereals, pulses, vegetables, and fruits. Also discussed are the principles of genetic engineering by which desired genes can be transferred from plants to animals to microorganisms and vice versa.

This edited book is an in-depth compilation of recent tools and techniques, concepts and strategies used globally in plant molecular farming (PMF) for the cost-effective bulk production of recombinant proteins, secondary metabolites, and other biomolecules. The book presents an overview of success stories of PMF applications from developing countries to address poverty, achieve zero hunger, good health and well-being, thus achieving the UN SDGs 1, 2, and 3.The book deep dives into recent extraction and downstream processing methodologies, its co-existence with conventional agriculture, global governance and finally opportunities, challenges, and future perspectives in plant molecular farming. It focuses on plastid/chloroplast transformation (transplastomics) and its application in plant molecular farming. The books highlight recent advances in genome editing, synthetic biology, glycosylation and glyco-engineering for improved plant molecular farming by marker-free andtissue-specific systems via cisgenic and transgenic crops. In depth discussions on biosafety issues and bio-containment strategies have also been included.The book has 15 chapters authored by globally leading experts on the subject, presenting opportunities & challenges for bio-industrial researchers and entrepreneurs. It is useful to researchers, industrialists, entrepreneurs, policy planners, academician, and students across the disciplines.

With over 820 million people facing hunger in today's world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops

Grassland covers 26% of the world's total land area. It produces feed for livestock; maintains soil fertility; protects and conserves soil and water resources; creates a habitat for wildlife; provides recreational space for sport and leisure and contributes to the general landscape. This book provides an up-to-date account of progress and potential in the genetic improvement of grassland to meet all needs. It encompasses work on a wide range of temperate and tropical grassland species (including grasses, clovers and other forage legumes) and will interest all those concerned with grassland use in livestock-based agriculture, recreation, environmental protection, bio-industry etc. Specifically, it demonstrates how recent advances in molecular techniques are being used to develop breeding objectives and strategies with key-note papers on: Objectives and benefits of molecular breeding, Linkage/physical mapping and map-based cloning, QTL analysis and trait dissection, Genomics, model species, gene discovery and functional analysis, Use of molecular markers and bioinformatics for breeding, Molecular genetics and breeding of endosymbiont and grass/legume associations, Transgenics, Genetic diversity, breeding systems and resources Future directions for research and breeding. State-of-the-art molecular techniques and resources are described that encompass a unique range of expertise in genetic mapping, trait dissection, comparative genomics, bioinformatics, gene discovery and risk assessment. Examples of work in progress or recently completed are provided from across the world. The book has broad educational value and will interest plant geneticists and breeders as well as grassland users and policy makers.

Maize is one of the most generally grown cereal crops at global level, followed by wheat and rice. Maize is the major crop in China both in terms of yield and acreage. In 2012, worldwide maize production was about 840 million tons. Maize has long been a staple food of most of the global population (particularly in South America and Africa) and a key nutrient resource for animal feed and for food industrial materials. Maize belts vary from the latitude 58° north to the latitude 40° south, and maize ripens every month of the year. Abiotic and biotic stresses are common in maize belts worldwide. Abiotic stresses (chiefly drought, salinity, and extreme temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, production and productivity. In the recent past, intense droughts, waterlogging, and extreme temperatures have relentlessly affected maize growth and yield. In China, 60% of the maize planting area is prone to drought, and the resultant yield loss is 20%–30% per year; in India, 25%–30% of the maize yield is lost as a result of waterlogging each year. The biotic stresses on maize are chiefly pathogens (fungal, bacterial, and viral), and the consequential syndromes, like ear/stalk rot, rough dwarf disease, and northern leaf blight, are widespread and result in grave damage. Roughly 10% of the global maize yield is lost each year as a result of biotic stresses. For example, the European corn borer [ECB, Ostrinianubilalis (Hübner)] causes yield losses of up to 2000 million dollars annually in the USA alone in the northern regions of China, the maize yield loss reaches 50% during years when maize badly affected by northern leaf blight. In addition, abiotic and biotic stresses time and again are present at the same time and rigorously influence maize production. To fulfill requirements of each maize-growing situation and to tackle the above mentions stresses in an effective way sensibly designed multidisciplinary strategy for developing suitable varieties for each of these stresses has been attempted during the last decade. Genomics is a field of supreme significance for elucidating the genetic architecture of complex quantitative traits and characterizing germplasm collections to achieve precise and specific manipulation of desirable alleles/genes. Advances in genotyping technologies and high throughput phenomics approaches have resulted in accelerated crop improvement like genomic selection, speed breeding, particularly in maize. Molecular breeding tools like collaborating all omics, has led to the development of maize genotypes having higher yields, improved quality and resilience to biotic and abiotic stresses. Through this book, we bring into one volume the various important aspects of maize improvement and the recent technological advances in development of maize genotypes with high yield, high quality and resilience to biotic and abiotic stresses

This book is exploring molecular insight of plant disease resistance, enhancing plant immunity as well as the latest omics or approaches in plant disease management. In the recent past, microbial strains or products frequently utilized to inhibit the growth of phytopathogen and disease management. However, it is well known that plants respond to numerous biotic and abiotic stresses by morphological, biochemical, and molecular mechanisms. But still there is much more to study about their molecular aspect of interaction between host- pathogens- biocontrol agents that will be helpful in formulation and applications of microbial antagonistic for effective management of phytopathogens. This book attempt to fill this gap in the literature. This book is of interest to teachers, researchers, agronomist, horticulturalist scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, microbiology, environment science.

This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the abiotic stresses caused by heat, cold, drought, flooding, submergence, salinity, acidity, etc., important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in technical crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing in many of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for'designing'crop genomes with resistance to abiotic stresses. The ten chapters each dedicated to a technical crop and one chapter devoted to a crop group in this volume elucidate different types of abiotic stresses and their effects on and interaction with the crops; enumerate the available genetic diversity with regard to abiotic stress resistance among available cultivars; illuminate the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing abiotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genesfor generating abiotic stress-resistant crops.

This book discusses genetic engineering of both plants and microbes for making agricultural practices more productive and sustainable. It chapters explore the understanding of the interaction between plants and microbes, and genomic information to modify the metabolism of plants or microbes to further enhance the beneficial interaction. The book covers the development of commercial inoculants including selection of appropriate plant growth-promoting rhizobacteria/ phosphate solubilize bacteria based on target host plant, soil type, indigenous microbial communities, environmental conditions, inoculant density, suitability of carriers and compatibility with integrated crop management. This is a relevant content for scientists and researchers working on soil biology, sustainable agricultural and plant physiology. Also, this book is a useful read for graduate and post graduate students of agriculture, botany and microbiology.

This volume explains the recent findings on the mutualistic plant–microbe interactions and how they can be utilized for sustainable agriculture practices including land reclamation. The book covers mainly plant growth promoting microorganisms (PGPMs) including both the symbiotic bacteria and fungi and their role in mobilization of nutrients, providing protection to the crops from phytopathogens and abiotic stresses. PGPMs play important roles in survival and health of the plant. These useful microorganisms provide plants with nutrients, protect them from pathogens and help them combat abiotic stresses. It is important that these mutualistic interactions between plant and soil microbes are well understood so as to develop reliable products in the form of biostimulants and biopesticides, as well as managing biotic and abiotic stresses in crops. Apart from enhancing crop productivity plant–microbe interactions can also perform activities such as reclamation of degraded lands, degradation of pollutants and remediation of saline or marginal lands. This book is of interest to teachers, researchers, plant scientists and microbiologists. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture, microbiology, biotechnology, ecology, soil science and environmental sciences.

This book covers the important diseases and pests of potato which are of global significance. The pests and diseases in potato lead to huge economic losses by reducing the yield and quality of the produce. This book describes major pests and diseases in detail with particular emphasis on the latest developments with respect to their biology, ecology, and management. It highlights the importance of virus infection for seed potato production and diagnostic symptoms, along with management guidelines. The book brings forth tips for judicious use of pesticides for sustainable potato production and management of pesticide resistance. Use of novel approaches such as RNA interference, genome editing, and other genomic resources for drug designing in diseases and pest management is also emphasized in the book. This book is of interest to teachers, researchers, extension workers, potato growers, and policy makers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture and plant pathology. National and international agricultural scientists and policy makers will also find this to be a useful read.

This book is an advanced textbook and a reference book for the post-graduate plant-breeding students and the plant breeders. It consolidates fundamental concepts and also the latest advances in plant-breeding practices including development in crop genomics. It contains crop wise explanation on origin, reproduction, genetics of yield contributing traits, biotic and abiotic stresses, nutritional improvement and crop specific plant-breeding procedures and techniques. The chapters are planned to describe crop-focused breeding procedure for the major crop plants as per their economic importance. The recent developments in breeding of field crops have been reported. The recent progress made in mapping traits of economic importance has been critically reviewed for each crop. The progress made in markers assisted selected in few crops has been summarized. This book bridges the knowledge gap and bring to the researchers and students information on modern breeding tools for developing biotic and abiotic stress tolerant, climate resilient and micronutrient rich varieties of field crops. The chapters in book are contributed by experienced Plant Breeders.

Biotic stresses cause yield loss of 31-42% in crops in addition to 6-20% during post-harvest stage. Understanding interaction of crop plants to the biotic stresses caused by insects, bacteria, fungi, viruses, and oomycetes, etc. is important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding and the recently emerging genome editing for developing resistant varieties in technical crops is imperative for addressing FHEE (food, health, energy and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing have facilitated precise information about the genes conferring resistance useful for gene discovery, allele mining and shuttle breeding which in turn opened up the scope for'designing'crop genomes with resistance to biotic stresses. The 15 chapters dedicated to 13 technical crops and 2 technical crop groups in this volume will deliberate on different types of biotic stress agents and their effects on and interaction with crop plants; will enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; will brief on the classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; will enunciate the success stories of genetic engineering for developing biotic stress resistant varieties; will discuss on molecular mapping of genes and QTLs underlying biotic stress resistance and their marker-assisted introgression into elite varieties; will enunciate on different emerging genomics-aided techniques including genomic selection, allele mining, gene discovery and gene pyramiding for developing resistant crop varieties with higher quantity and quality; and will also elaborate some case studies on genomeediting focusing on specific genes for generating disease and insect resistant crops.

Fungi are an important link in the food webs of all ecosystems. They have immense potential and comprise a myriad of useful bioactive compounds. Fungi feature in a wide range of diverse processes and applications in modern agriculture, the food science industry, and the pharmaceutical industry. In the food and drink arena, the role of fungi is historically important in the form of mushrooms and in fermented foods as yeasts for baking and brewing. These roles are supplemented by the use of fungal food processing enzymes and additives, and more recently in the development of protein-based foodstuffs from fungi. Additionally, they are used in the formulation of biofertilizers and biopesticides used as biostimulants and bioprotectants of crops. The practical use of newer techniques such as genetic recombination and robotics have revolutionized the modem agricultural biotechnology industry, and have created an enormous range of possible further applications of fungal products.Myco-materials created from mycelia (the root-like parts of fungi) are gaining attention as a sustainable alternative for a wide range of materials. They are being used as insulation, sustainable packaging, foam inserts, and even'eco-leather.” In fact, mycelium bricks are pound-for-pound stronger than concrete. In addition, medicinal uses of fungal species have been historically recorded as important agents in the pharmaceutical sciences. The potential for myco-materials seems limitless. The field of mycology and its application has become an increasingly important component in the education of industrial biotechnology. This book on applied mycology provides information helpful for developing entrepreneurial opportunities with fungi. This volume explains both the basic science and the applications of mycology and bio-resource technology with special emphasis on entrepreneurial applications. It offers a complete, one-stop resource for those interested in microbiology, food and agricultural science, medical mycology, and for those in industrial biotechnology.

Through this book, the readers will learn about the different aspects of Actinobacteria- beginning with its ecology and occurrence, to the ways of its adaptation to harsh climates, and finally to its practical applications. The book also presents methods of identifying and characterizing this diverse group of bacteria through advanced techniques like MALDI-TOF, 16S rRNA analysis, etc. Different chapters describe the various biotechnological applications of Actinobacteria, including bioremediation, secondary metabolite production, and in producing antibiotics, anti-cancer therapeutics. It also provides insights into the applications in agriculture and forestry by inhibiting plant pathogenic bacteria's growth.

This edited book presents the latest research on cucumber, its genetic resources and diversity, tissue culture and genetic transformation, mapping of economic genes and QTLs, whole genome sequencing, comparative genomics, and breeding strategies. The mechanism of sex expression, interspecific hybridization, and cell biology are also described. The book discusses the genome draft of cucumber and the application of genome editing. This book is useful to the students, teachers and scientists in academia and relevant private companies interested in horticulture, genetics, breeding, and related areas.

This book becomes an invaluable reference on the genetic resources, genome, genes, chemical compounds, and their therapeutic effects for the Lingzhi mushrooms. It is the first comprehensive compilation of genetic resources, nuclear genome, mitochondrial genome, genes, noncoding RNAs, such as long intergenic noncoding RNAs, microRNAs, circular RNAs, genes in the biosynthetic pathway, chemical compounds and their therapeutic effects, transformation system for the expression of key genes, a bibliometric analysis to identify the past research work and the future research direction, and a survey of products derived from the Lingzhi mushrooms. Each chapter of this book is written by authors of globally reputed experts on the relevant field who had published high-quality articles in the corresponding subject. The book has 12 chapters and each chapter has a length of approximately ten thousand words, including ten items (tables or figures), about 30–50 references. This book isuseful to the students, teachers, and scientists in academia and relevant private companies interested in horticulture, genetics, physiology, molecular genetics, and breeding, in vitro culture and genetic engineering, and structural and functional genomics. This book is also useful to seed and pharmaceutical industries.

Blast is an important foliar disease that infects the majority of cereal crops like rice, finger millet, pearl millet, foxtail millet and wheat, and thus resulting in a huge economic impact. The pathogen is responsible for causing epidemics in many crops and commonly shifts to new hosts. Magnaporthe spp. is the most prominent cause of blast disease on a broad host range of grasses including rice as well as other species of poaceae family. To date, 137 members of Poaceae hosting this fungus have been described in Fungal Databases. This book provides information on all blast diseases of different cereal crops. The pathogen evolves quickly due to its high variability, and thus can quickly adapt to new cultivars and cause an epidemic in a given crop. Some of the topics covered here include historical perspectives, pathogen evolution, host range shift, cross-infectivity, and pathogen isolation, use of chemicals fungicides, genetics and genomics, and management of blast disease in different cereal crops with adoption of suitable methodologies.In the past two decades there have been significant developments in genomics and proteomics approaches and there has been substantial and rapid progress in the cloning and mapping of R genes for blast resistance, as well as in comparative genomics analysis for resolving delineation of Magnaporthe species that infect both cereals and grass species. Blast disease resistance follows a typical gene-for-gene hypothesis. Identification of new Avr genes and effector molecules from Magnaporthe spp. can be useful to understand the molecular mechanisms involved in the fast evolution of different strains of this fungal genus. Advances in these areas may help to reduce the occurrence of blast disease by the identification of potential R genes for effective deployment. Additionally, this book highlights the importance of blast disease that infects different cereal hosts in the context of climate change, and genomics approaches that may potentially help in understanding and applying new concepts and technologies that can make real impact in sustainable management of blast disease in different cereal crops.

This book focuses on the application of microorganisms in various aspects of life such as plant protection and improvement, environmental remediation, and the improvement of plant & human health. Various applications of microorganisms are examined in depth, e.g. applied microbiology in agriculture, microbes in the environment, the development of new microbial enzymes, and microbes in human health. In turn, the book shares insights into the diverse microorganisms that have been explored and exploited in the development of various applications for agricultural improvements. It also discusses the detection and exploitation of microorganisms in the diagnosis of human diseases, which offer potential holistic approaches to health. Presenting the latest information and findings on the applications of microbial biotechnology, the book offers a valuable resource.

Mulberry (Morus spp.) is an important horticultural plant in the sericulture industry. It belongs to the family Moraceae. The leaf of mulberry is used to feed the silkworm Bombyx mori L. It is also used as a fodder. Due to its economic and agricultural importance, mulberry is cultivated in many parts of the world. An estimated 60% of the total cost of silk cocoon production is for production and maintenance of mulberry plants. Therefore, much attention is needed to improve the quality and quantity of mulberry leaves. It is vital to increase the production of superior quality mulberry leaves with high nutritive value for the sericulture industry. Although a lot of research is going on in mulberry, very little effort has been made to compile the results of this research in a single book. This book provides an update of recent research works going on in this plant. It describes the taxonomy, conservation of germplasm, genetic diversity of various mulberry species, application of breeding techniques to improve the quality of mulberry, in vitro conservation, application of tissue culture techniques to improve mulberry species, production of haploids and triploids in mulberry and improvement of abiotic stress adaptive traits in mulberry with relevance to adaptiveness to global warming.

This book is the first comprehensive compilation of deliberations on whole genome sequencing of sesame including genome assembly, annotation, structure and synteny analysis, and sequencing of its chloroplast genome and also its wild species. It presents narratives on classical genetics and breeding, tissue culture and genetic transformation, molecular mapping and breeding. Other chapters describe the beneficial components in sesame protein and oil, botanical depictions and cytological features. Prospects of designed breeding in the post-genomics era including gene discovery have also been enumerated.Altogether, the book contains 19 chapters authored by globally reputed experts on the relevant field in this crop. This book is useful to the students, teachers, and scientists in the academia and relevant private companies interested in classical and molecular genetics, biotechnology, breeding, biochemistry, traditional and molecular breeding, and structural and evolutionary genomics. The work is also useful to seed and oil industries.

Genetic transformation is a key technology, in which genes are transferred from one organism to another in order to improve agronomic traits and ultimately help humans. However, there is apprehension in some quarters that genetically modified crops may disturb the ecosystem. A number of non-governmental organizations continue to protest against GM crops and foods, despite the fact that many organisms are genetically modified naturally in the course of evolution. In this context, there is a need to educate the public about the importance of GM crops in terms of food and nutritional security. This book provides an overview of various crop plants where genetic transformation has been successfully implemented to improve their agronomically useful traits. It includes information on the gene(s) transferred, the method of gene transfer and the beneficial effects of these gene transfers and agronomic improvements compared to the wild plants. Further, it discusses the commercial prospects of these GM crops as well as the associated challenges. Given its scope, this book is a valuable resource for agricultural and horticultural scientists/experts wanting to explain to the public, politicians and non-governmental organizations the details of GM crops and how they can improve crops and the lives of farmers.

This volume provides a comprehensive overview of the major applications and potential of fungal biotechnology. The respective chapters report on the latest advances and opportunities in each topic area, proposing new and sustainable solutions to some of the major challenges faced by modern society. Aimed at researchers and biotechnologists in academia and industry, it represents essential reading for anyone interested in fungal biotechnology, as well as those working within the broader area of microbial biotechnology. Written in an accessible language, the book also offers a valuable reference resource for decision-makers in government and at non-governmental organizations who are involved in the development of cleaner technologies and the global bioeconomy. The 21st century is characterized by a number of critical challenges in terms of human health, developing a sustainable bioeconomy, facilitating agricultural production, and establishing practices that support a cleanerenvironment. While there are chemical solutions to some of these challenges, developing bio-based approaches is becoming increasingly important. Filamentous fungi, ‘the forgotten kingdom,'are a group of unique organisms whose full potential has yet to be revealed. Some key properties, such as their exceptional capacity to secrete proteins into the external environment, have already been successfully harnessed for the production of industrial enzymes and cellulosic biofuels. Many further aspects discussed here –such as feeding the hungry with fungal protein, and the potential applications of the various small molecules produced by fungi –warrant further exploration. In turn, the book covers the use of fungal cell factories to produce foreign molecules, e.g. for therapeutics. Strategies including molecular approaches to strain improvement, and recent advances in high-throughput technologies, which are key to finding better products and producers, are also addressed. Lastly, the book discusses the advent of synthetic biology, which is destined to greatly expand the scope of fungal biotechnology. The chapter “Fungal Biotechnology in Space: Why and How?” is available open access under a Creative Commons Attribution 4.0 International License at link.springer.com.

This book focuses on the conventional breeding approach, and on the latest high-throughput genomics tools and genetic engineering / biotechnological interventions used to improve rice quality. It is the first book to exclusively focus on rice as a major food crop and the application of genomics and genetic engineering approaches to achieve enhanced rice quality in terms of tolerance to various abiotic stresses, resistance to biotic stresses, herbicide resistance, nutritional value, photosynthetic performance, nitrogen use efficiency, and grain yield. The range of topics is quite broad and exhaustive, making the book an essential reference guide for researchers and scientists around the globe who are working in the field of rice genomics and biotechnology. In addition, it provides a road map for rice quality improvement that plant breeders and agriculturists can actively consult to achieve better crop production.

This fully revised third edition includes up-to-date topics and developments in the field, which has made tremendous strides since the publication of the second edition in 2004. Many novel techniques based on Next Generation Sequencing have sped up the analysis of fungi and major advances have been made in genome editing, leading to a deeper understanding of the genetics underlying cellular processes as well as their applicability. At the same time, the relevance of fungi is unbroken, both due to the serious threats to human health and welfare posed by fungal pests and pathogens, and to the many benefits that fungal biotechnology can offer for diverse emerging markets and processes that form the basis of the modern bioeconomy. With regard to these advances, the first section of this volume, Genetics, illustrates the basic genetic processes underlying inheritance, cell biology, metabolism and “lifestyles” of fungi. The second section, Biotechnology, addresses theapplied side of fungal genetics, ranging from new tools for synthetic biology to the biotechnological potential of fungi from diverse environments. Gathering chapters written by reputed scientists, the book represents an invaluable reference guide for fungal biologists, geneticists and biotechnologists alike.

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commerical export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.

This book summarizes the various areas of research in metagenomics and their potential applications in medicine, the environment and biotechnology. The book presents the recent advances in theoretical, methodological and applied aspects of metagenomics and highlights their applications in the fields of environmental microbial forensics, bioremediation, drug-discovery and agriculture. In addition, the book discusses various metagenomics approaches used for understanding the microbial physiology and biochemistry. Lastly the book describes a range of bioinformatics tools and computational methods for metagenomics analysis as well as the functional diversity and dynamics of microbial communities colonizing the human skin.

This book compiles the fundamental advances resulting from of oil-palm genome and transcriptome sequencing, and describes the challenges faced and strategies applied in sequencing, assembling and annotating oil palm genome sequences. The availability of genome and transcriptome data has made the mining of a high number of new molecular markers useful for genetic diversity as well as marker-trait association studies and the book presents high-throughput genotyping platforms, which allow the detection of QTL regions associated with interesting oil palm traits such as oil unsaturation and yield components using classical genetic and association mapping approaches. Lastly, it also presents the discovery of major genes governing economically important traits of the oil palm.Covering the history of oil palm expansion, classical and molecular cytogenetics, improvements based on wild and advanced genetic materials, and the science of oil palm breeding, the book is a valuable resourcefor scientists involved in plant genetic research.

The green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in genetic engineering, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenics, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. This book discusses the commercial applications of plant transgenic technologies, including the use of transgenic cell culture approachesto improve the production of metabolites and high-value therapeutics as well as transgenic plants in pest management. It also explores generation of novel vectors, protein production using chloroplast engineering and the latest developments in this area, such as genome editing in plants. Featuring general discussions and research papers by leading international experts, it is a valuable resource for scientists, teachers, students and industrialists working in the field.

Advances in molecular biology and genome research in the form of molecular breeding and genetic engineering put forward innovative prospects for improving productivity of many pulses crops. Pathways have been discovered, which include regulatory elements that modulate stress responses (e.g., transcription factors and protein kinases) and functional genes, which guard the cells (e.g., enzymes for generating protective metabolites and proteins). In addition, numerous quantitative trait loci (QTLs) associated with elevated stress tolerance have been cloned, resulting in the detection of critical genes for stress tolerance. Together these networks can be used to enhance stress tolerance in pulses. This book summarizes recent advances in pulse research for increasing productivity, improving biotic and abiotic stress tolerance, and enhancing nutritional quality.

Cell Biology and Genetics give an integrated picture of the genetic material, its behavior and transmission in heredity. Molecular Biology is largely a component of Cell Biology and Genetics which include the study of structure, function of cell molecules and the pathways they follow. The book attempts to provide the students with the basics of Cell Biology, Genetics and Molecular Biology, the laws they follow, and the strategy of their application. The subject matter is concise with the latest information bearing necessary diagrams, tables, examples and photographs.

Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.

This book examines the development of innovative modern methodologies towards augmenting conventional plant breeding, in individual crops, for the production of new crop varieties under the increasingly limiting environmental and cultivation factors to achieve sustainable agricultural production, enhanced food security, in addition to providing raw materials for innovative industrial products and pharmaceuticals. This is Vol 7, subtitled Legumes, focuses on advances in breeding strategies using both traditional and modern approaches for the improvement of individual legume crops. Included in this volume are Adzuki bean, Black gram, Chickpea Cluster bean, Common bean, Cowpea, Faba bean, Hyacinth bean, Lentil, Mung bean, Pigeonpea and Soybean. This volume is contributed by 57 internationally reputable scientists from 9 countries. Each chapter comprehensively reviews the modern literature on the subject and reflects the authors own experience.

This book highlights modern strategies and methods to improve oilseed crops in the era of climate change, presenting the latest advances in plant molecular breeding and genomics-driven breeding. Spectacular achievements in the fields of molecular breeding, transgenics and genomics in the last three decades have facilitated revolutionary changes in oilseed- crop-improvement strategies and techniques. Since the genome sequencing of rice, as the first crop plant, in 2002, the genomes of about one dozen oilseed crops have been sequenced and more are to follow. This has made it possible to decipher the exact nucleotide sequence and chromosomal positions of agroeconomic genes. Most importantly, comparative genomics and genotyping-by-sequencing have opened up new vistas for exploring available biodiversity, particularly of wild crop relatives, for identifying useful donor genes.

This book is the first comprehensive volume on the genetics and genomics of pineapple and provides an overview of the current state of pineapple research. Pineapple [Ananas comosus (L.) Merr.] is the second most important tropical fruit after banana in term of international trade. Its features are advantageous for genomic research: it has a small genome of 527 Mb which is diploid and vegetatively propagated; it is monocot, closely related to the grass family that includes major cereal crops, wheat, rice, corn, sorghum, and millet; and it serves as an out group for genetic and genomic research in grasses. In addition to exploring the evolution and improvement of pineapple, this work examines the pineapple genome with respect to genome structure and organization, comparative analyses with other angiosperm genomes, transcription factors, disease resistance, and circadian clock regulation of CAM related genes. With chapters covering botanical, genetic, genomic, and applied aspects ofpineapple, this text also encourages the application of genomic technologies and suggests future prospects.

This new volume provides a better understanding of molecular plant breeding in order to boost the quality of agriculture produce, to increase crop yields and to provide nutritious food for everyone by 2050. Scientists believe the challenge can be met by implementing new and improved techniques of quantitative trait inheritance in plant breeding. Integrating genomics and molecular biology into appropriate tools and methodologies can help to create genetically engineered plants, such as by using biotic and abiotic stress tolerance, molecular markers, ‘-omics'technology, and genome editing.

This book addresses various aspects of the current castor bean research, including genetics, biotechnology, comparative genomics, and more specific topics such as oil metabolism and the ricin toxin. It also presents the whole genome sequencing of the castor bean and its impact on the mining of gene families and future plant breeding. Castor bean (Ricinus communis), an oilseed plant, belongs to the Euphorbiaceae (spurge) family. It is a tropical and subtropical crop valued for the high quality and uniform nature of its oil, which is mostly composed of the uncommon fatty acid ricinoleate. Castor bean oil has important industrial applications for the production of lubricants, cosmetics, medicines, and specialty chemicals, and castor bean has also been proposed as a biodiesel crop that does not pose concerns regarding the “food versus fuel” debate. However, it accumulates the type 2 ribosome-inactivating protein ricin in its seeds, and health concernsposed by ricin's high toxicity have prevented broader cultivation. Recently, there has been renewed interest in castor bean due to potential biosecurity issues.

Plant molecular biology came to the fore in the early 1980s and there has been tremendous growth in the subject since then. The study of plant genes and genomes, coupled with the development of techniques for the incorporation of novel or modified genes into plants, eventually led to the commercialisation of genetically modified (GM) crops in the mid-1990s. This was seen as the start of a biotechnological revolution in plant breeding. However, plant biotechnology became one of the hottest debates of the age and, in Europe at least, has been mired in controversy and over-regulation. Nevertheless, recent years have seen further technological innovation in the development of a range of techniques that enable scientists to make specific changes to target genes.Through a detailed history and development of the science and techniques that underpin crop biotechnology, this title is concise, comprehensive and readable. As well as new sections on genome editing, this edition includes expanded sections on current GM crops and future developments in plant biotechnology, and updated sections on techniques, legislation and the GM crop debate.The previous edition of this book, titled Genetically Modified Crops, 2nd Edition, was published in November 2011.

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen “case studies of important plant crops” intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

This book concerned with the importance of genetically modified organism to plants and human. The book concerned also on what is gene, structure of gene and the role of gene in heredity. The role of DNA in plants and its structure is discussed. Synthesis of protein and role of DNA and RNA in this process. A genetically modified organism (GMO) means that any organism whose genetic material has been altered using genetic engineering techniques. GMO crops are now commercially planted in some 22 developed and developing countries.

This book provides in-depth insights into the biology, taxonomy, genetics, physiology and biotechnological applications of Actinobacteria. It especially focuses on the latter, reviewing the wide variety of actinobacterial bioactive molecules and their benefits for diverse industrial applications such as agriculture, aquaculture, biofuel production and food technology. Actinobacteria are one of the most promising sources of small bioactive molecules and it is estimated that only a small percentage of actinobacterial bioactive chemicals have been discovered to date. Identifying new diverse gene clusters of biotechnological relevance in the genome of Actinobacteria will be crucial to developing advanced applications for pharmaceutical, industrial and agricultural purposes. The book offers a unique resource for all graduate students, researchers and practitioners in the fields of microbiology, microbial biotechnology, and the genetic engineering of Actinobacteria.