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Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more. - Contains contributions from experts in the field from across the world - Covers a wide array of topics on both mitosis and meiosis - Includes relevant, analysis based topics

This book critically evaluates the causal link between cell division machinery and disease. Further, it identifies key open questions in the field and the means for exploring them. Throughout the various chapters, internationally known contributors present the evidence for and against a causal link between key elements of the cell division machinery and diseases such as cancer, neuropathologies, aging, and infertility. A more clinically oriented chapter further discusses the current and future applications of anti-mitotic drugs in these diseases. Cell Division Machinery and Disease is essential reading for graduate or advanced graduate students, researchers or scientists working on cell division as well as clinicians interested in the molecular mechanisms of the discussed diseases.

This book provides readers with an overview of the frequent occurrence of asymmetric cell division. Employing a broad range of examples, it highlights how this mode of cell division constitutes the basis of multicellular organism development and how its misregulation can lead to cancer.To underline such developmental correlations, readers will for example gain insights into stem cell fate and tumor growth. In turn, subsequent chapters include descriptions of asymmetric cell division from unicellular organisms to humans in both physiological and pathological conditions. The book also illustrates the importance of this process for evolution and our need to understand the background mechanisms, offering a valuable guide not only for students in the field of developmental biology but also for experienced researchers from neighboring fields.

This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells. - Covers sections on model systems and functional studies, imaging-based approaches and emerging studies - Chapters are written by experts in the field - Cutting-edge material

The molecular mechanisms controlling cell cycle progression are highly conserved in eukaryotes. In addition to the basic protein machinery involved in cell cycle regulation, higher plants have also evolved unique molecular mechanisms that allow integration of environmental, physiological, and developmental signals into networks to control proper cell division and expansion. Rapid and exciting research progress in these fields has been achieved from experimental observations on plants over the past decade. The scope of this volume is focused on the molecular basis of all aspects of cell division and cytokinesis in plants. It is an essential reference book for instructors and scientists working in the areas of molecular, cell, and developmental biology of plants. The editors of this book are veterans in the field of plant molecular biology and highly respected worldwide.

Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation and processing through to locating and quantifying cells in different stages of the cell cycle. This book is an essential and comprehensive practical guide to these important and expanding areas.

Centrosomes play an integral role in the growth of cells and the ultimate development of many animals, and sometimes plants. In addition to the normal growth of cells, centrosomes can also play a key role in the spread of cancer and are of increased interest to both the genetics and oncology communities. Volume 49 of Current Topics in Developmental Biology will present all known research surrounding the centrosome, across a variety of systems, will be well referenced, and speculate where the research is headed. - Discusses centrosomes and cancer, centrosomes and early development, and molecular biology of the centrosome - Heavily illustrated, with many color figures - Chapters written by international leaders in the field

Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art'how to'secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle.Chapters Contain Information On:• How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies• Techniques to best image fluorescent and nonfluorescent tagged dividing cells• The use and action of mitoclastic drugs• How to generate antibodies to mitotic components and inject them into cells• Methods that can also be used to obtain information on cellular processes in nondividing cells