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Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume One provides effective ways for organizing precision and sustainable agriculture. The methods include the use of advanced molecular techniques covering multiple omics, high-throughput technology, computational biology, epigenetic manipulation, and CRISPR genome editing. These methods can advance the development of high-yield, high-quality, and stress-resilient crops that meet the requirements for supporting global food and nutrition security.The book proposes strategies for omics-assisted and speed breeding techniques, exploring molecular mechanisms of plant abiotic stress caused by temperature, drought, salinity, and various pollutants. These are uncovered by quantitative trait loci analysis and mapping, genomic selection, functional genomics, multiple omics, high-throughput sequencing, and high-throughput phenotyping, and are integrated into the various systems of crop improvement.Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume One presents emerging and comprehensive knowledge and is an ideal reference for students, researchers, teachers, and professors. It inspires ideas for investigations in the fields of plant stress physiology, plant functional genomics, plant multiple omics, plant genetic engineering, systems biology, and crop breeding.

This book deliberates on the concept, strategies, tools, and techniques of allele mining in grain legume crops and its application potential in genome elucidation and improvement, including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and also studies of gene expression and gene prediction. Available gene pools in global germplasm collections specifically consisting of wild allied species and local landraces for almost all major crops have facilitated allele mining. Development of advanced genomic techniques, including PCR-based allele priming and EcoTILLING-based allele mining, is being widely used now for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs, and also with genome plasticity to adapt to climate change scenarios. All these concepts and strategies, along with precise success stories, are presented in the chapters dedicated to the major grain legume crops.1. The first book on the novel strategy of allele mining in grain legume crops for precise breeding.2. Presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources.3. Depicts case studies of PCR-based allele priming and EcoTILLING-based allele mining.4. Elaborates on gene discovery and gene prediction in major grain legume crops.This book will be useful to students and faculties in various plant science disciplines, including genetics, genomics, molecular breeding, agronomy, and bioinformatics; to scientists in seed industries; and also to policymakers and funding agencies interested in crop improvement.

This book covers the biotechnological advances being used for the understanding of plant diseases and the subsequent enhancement of disease resistance in crop plants. Chapters are focused on recent advances in sequencing technologies, computational resources and genomics tools useful for the identification of loci governing disease resistance. In addition, emphasis is given to novel approaches like genomic selection for achieving significant genetic gain for quantitative disease resistance. The book thoroughly describes sequencing-based approaches like whole genome sequencing, resequencing, and transcriptome profiling being explored for the understanding of disease resistance mechanisms. Finally, several chapters systematically describing the utilities and concerns of high-end technologies like genome-editing are provided. Simplified Illustrations are provided in every chapter to explain different biotechnological approaches and strategies. The book will help to better explore the biotechnological advances in development of disease resistant crop varieties.

This book deliberates on the concept, strategies, tools, and techniques of allele mining in vegetable crops and its application potential in genome elucidation and improvement including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and studies of gene expression and gene prediction. Available gene pools in global germplasm collections specifically consisting of wild allied species and local landraces for almost all major crops have facilitated allele mining. Development of advanced genomic techniques including PCR-based allele priming and Eco-TILLING based allele mining are being widely used now for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs and with genome plasticity to adapt the climate change scenarios. All these concepts and strategies along with precise success stories are presented in the chapters dedicated to the major vegetable crops. The first book on the novel strategy of allele mining in vegetable crops for precise breeding Presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources Depicts case studies of PCR-based allele priming and Eco-TILLING based allele mining Elaborates on gene discovery and gene prediction in major vegetable crops This book will be useful to the students and faculties in various plant science disciplines including genetics, genomics, molecular breeding, agronomy, and bioinformatics; the scientists in seed industries; and, the policymakers and funding agencies interested in crop improvement.

This edited volume presents recent advancements in genetic transformation and genome editing, offering a comprehensive understanding of their applications for creating more sustainable crops. These innovations are revolutionizing crop development, enhancing yields, and mitigating environmental challenges.The demand for sustainable crop production, driven by a growing global population and mounting environmental challenges, has never been more pressing. Genetic transformation and genome editing techniques offer precise and targeted ways to enhance crop traits, increase yields, and reduce the need for chemical inputs. The book traces the evolution of these technologies, from the early days of genetic modification to the current era of CRISPR-based genome editing. It covers cutting-edge technologies, from CRISPR-Cas9 to advanced gene editing techniques, while discussing the current scenario and future prospects of GM crops developed either by transgenic or genome editing approaches. The book explores the ethical and regulatory aspects surrounding genetic transformation, providing a complete perspective on this evolving domain.It is an essential read for researchers, students, and professionals in the fields of agriculture, plant sciences, biotechnology, agronomy, as well as policymakers.

This book deliberates on the concept, strategies, tools, and techniques of allele mining in cereal crops and its application potential in genome elucidation and improvement, including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and studies of gene expression and gene prediction. Available gene pools in global germplasm collections specifically consisting of wild allied species and local landraces for almost all major crops have facilitated allele mining. Development of advanced genomic techniques including PCR-based allele priming and Eco-TILLING-based allele mining are being widely used now for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs and with genome plasticity to adapt the climate change scenarios. All these concepts and strategies along with precise success stories are presented in the chapters dedicated to the major cereal crops. The first book on the novel strategy of allele mining in cereal crops for precise breeding Presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources Depicts case studies of PCR-based allele priming and Eco-Tilling-based allele mining Elaborates on gene discovery and gene prediction in major cereal crops This book will be useful to the students and faculties in various plant science disciplines including genetics, genomics, molecular breeding, agronomy, and bioinformatics; the scientists in seed industries; and the policymakers and funding agencies interested in crop improvement.

With over 820 million people facing hunger in today's world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops

Maize is one of the most generally grown cereal crops at global level, followed by wheat and rice. Maize is the major crop in China both in terms of yield and acreage. In 2012, worldwide maize production was about 840 million tons. Maize has long been a staple food of most of the global population (particularly in South America and Africa) and a key nutrient resource for animal feed and for food industrial materials. Maize belts vary from the latitude 58° north to the latitude 40° south, and maize ripens every month of the year. Abiotic and biotic stresses are common in maize belts worldwide. Abiotic stresses (chiefly drought, salinity, and extreme temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, production and productivity. In the recent past, intense droughts, waterlogging, and extreme temperatures have relentlessly affected maize growth and yield. In China, 60% of the maize planting area is prone to drought, and the resultant yield loss is 20%–30% per year; in India, 25%–30% of the maize yield is lost as a result of waterlogging each year. The biotic stresses on maize are chiefly pathogens (fungal, bacterial, and viral), and the consequential syndromes, like ear/stalk rot, rough dwarf disease, and northern leaf blight, are widespread and result in grave damage. Roughly 10% of the global maize yield is lost each year as a result of biotic stresses. For example, the European corn borer [ECB, Ostrinianubilalis (Hübner)] causes yield losses of up to 2000 million dollars annually in the USA alone in the northern regions of China, the maize yield loss reaches 50% during years when maize badly affected by northern leaf blight. In addition, abiotic and biotic stresses time and again are present at the same time and rigorously influence maize production. To fulfill requirements of each maize-growing situation and to tackle the above mentions stresses in an effective way sensibly designed multidisciplinary strategy for developing suitable varieties for each of these stresses has been attempted during the last decade. Genomics is a field of supreme significance for elucidating the genetic architecture of complex quantitative traits and characterizing germplasm collections to achieve precise and specific manipulation of desirable alleles/genes. Advances in genotyping technologies and high throughput phenomics approaches have resulted in accelerated crop improvement like genomic selection, speed breeding, particularly in maize. Molecular breeding tools like collaborating all omics, has led to the development of maize genotypes having higher yields, improved quality and resilience to biotic and abiotic stresses. Through this book, we bring into one volume the various important aspects of maize improvement and the recent technological advances in development of maize genotypes with high yield, high quality and resilience to biotic and abiotic stresses

This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN's sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.

This book highlights the recent progress on the applications of mutation breeding technology in crop plants. Plant breeders and agriculturists are faced with the new challenges of climate change, human population growth, and dwindling arable land and water resources which threaten to sustain food production worldwide. Genetic variation is the basis which plant breeders require to produce new and improved cultivars. The understanding of mutation induction and exploring its applications has paved the way for enhancing genetic variability for various plant and agronomic characters, and led to advances in gene discovery for various traits. Induced mutagenesis has played a significant role in crop improvement and currently, the technology has resulted in the development and release of more than 3600 mutant varieties in most of the crop plants with great economic impact. The field of ‘mutation breeding'has come long way to become an important approach for crop improvement. This book covers various methodologies of mutation induction, screening of mutants, genome editing and genomics advances and mutant gene discovery. The book further discusses success stories in different countries and applications of mutation breeding in food crops, horticultural plants and plantation crops.This informative book is very useful to plant breeders, students and researchers in the field of agriculture, plant sciences, food science and genetics.