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Metalloproteins and Motor Proteins, Volume 141 focuses on recent advances in studying metalloproteins and motor proteins, along with their roles in different pathologies and drug-resistance. Chapters include Transcriptomic Analysis Reveals Zinc-Mediated Virulence and Pathogenicity in Multidrug-Resistant Acinetobacter baumannii, The Mechanistic Insights into Different Aspects of Promiscuity in Metalloenzymes, Role of Metal ion in ion channel Mechanisms, A model of microtubule depolymerization by kinesin-8 motor proteins, Metalloproteins and metalloproteomics in health and disease, Decoding Genetic and Pathophysiological Mechanisms in Amyotrophic Lateral Sclerosis and Primary Lateral Sclerosis: A Comparative Study of Differentially Expressed Genes and Implicated Pathways in Motor Neuron Disorders, and much more.Additional chapters cover Exploring the effect of disease causing mutations in metal binding sites of human ARSA in Metachromatic Leukodystrophy, Role of transmembrane proteins in regulating metalloproteins and motor proteins in human cancer, Role of transmembrane proteins in metalloproteins and motor proteinsin human neurogenesis, Role of transmembrane proteins in metalloproteins and motor proteins in human aging, Vesicle transport of metalloproteinases, Motor proteins and spermatogenesis, and much more. - Integrates experimental and computational methods for studying structure and function of metalloproteins and motor proteins and their implication in drug design - Presents timely chapters written by well-renowned authorities in their field - Contains a high number of high quality illustrations, figures, and tables and targets a very wide audience of specialists, researchers, and students

Sulfurtransferases: Essential Enzymes for Life stands as the first comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field from the Editors, each chapter covers a specific sulfurtransferase, including its basic biology and roles in healthy functioning, disease, drug discovery, and other biotechnological applications. The physiological function for each enzyme is considered in depth, along with regulation mechanisms, pharmacological inhibitors, and pathology and conditions related to altered enzymatic activity. Sulfurtransferases discussed include rhodanese, MST, thiosulfate-thiol sulfurtransferase, tRNA uracil 4-sulfurtransferase, thiosulfate-dithiol sulfurtransferases, biotin synthase, cysteine desulfurase, lipoyl synthase, molybdenum cofactor sulfurtransferase, thiazole synthase, molybdopterin synthase sulfurtransferase, molybdopterin synthase, tRNA-uridine 2-sulfurtransferase, tRNA-5-taurinomethyluridine 2-sulfurtransferase, tRNA-5-methyluridine (54) 2-sulfurtransferase, and L-aspartate semialdehyde sulfurtransferase, among others. Here, researchers will discover key knowledge and recent advances to bring forward new studies on this increasingly relevant class of enzymes, while clinicians may apply new findings in medical practice. - Brings together basic knowledge and applied research across a wide range of sulfurtransferases - Considers biochemical functioning, enzymatic regulation and activity, and related pathologies for each enzyme - Features chapter contributions from global leaders in the field

This book overviews purinergic receptors that are playing key roles in human and pathophysiological processes. The book elaborates on how selective P1 and P2 modulators have been developed as therapeutics for a variety of diseases. It also provides an overview of current perspectives in the design of purinergic receptor modulators and future challenges such as the availability of selective ligands for all receptor subtypes.Divided into 12 chapters, this comprehensive volume also offers a multidisciplinary perspective on the historical evolution, starting with a chapter devoted to the roots and early discoveries of adenosine and its receptors, followed by a twenty-year retrospective on the synthesis, properties, and functional potential of adenosine receptor ligands, probes, and functional conjugates. In the next chapters, experts in the field delve into topics such as the therapeutic potential of adenosine receptor ligands in wound healing and fibrosis, the therapeutic benefits of A2A receptor antagonists, the A2B adenosine receptor as a target for brain ischemia or demyelination, the development and latest advancements in clinical trials of A3 adenosine receptor ligands. Other chapters describe bifunctional tools to study adenosine receptors, allosteric modulators of adenosine receptors, and new computational approaches to inspect adenosine receptor-ligand recognition processes. Furthermore, the book discusses the role of P2X4 receptors in immunity and inflammation. The final chapters illustrate CD73 inhibitors as antitumor agents, and bacterial ectonucleotidases as underexplored antibacterial drug targets. This book is a valuable resource for scholars working in the field of medicinal chemistry, as well as researchers in the industry, providing readers with a comprehensive understanding of adenosine receptor biology and its therapeutic potential.

This fully updated and expanded second edition of a highly popular text book focuses on the structure and mechanism in carbohydrate chemistry and biochemistry. Carbohydrates play important roles in biological systems as energy sources, as structural materials, and as informational structures (when they are often attached to proteins or lipids). Their chemical reactivity and conformational behaviour is governed by mechanistic and stereochemical rules, which apply as much to enzymic as to non-enzymic reactivity. The same principles of reactivity and conformation govern changes brought about in the process industries, such as pulp, paper and food. Extensively referenced with citations and a detailed index, the book contains everything the reader needs to know to start a carbohydrate research project with one of the real strengths being the treatment and integration of the important physical-chemical principles and methods (though lead references only are given to the finer points of carbohydrate synthesis). The book is suitable for both researchers who are new to the subject and those more established as well as a readership from diverse backgrounds and interests, including chemists, biochemists, food scientists and technologists involved with the processing of polysaccharides in the paper, textile, cosmetics, biofuels and other industries.

Carbon materials play a significant role in the development of alternative clean and sustainable energy technologies. This new volume focuses on the new applications of different carbon nanomaterials and graphene-carbon-nanotube hybrids for energy generation, energy storage, and energy conversion. It presents a comprehensive overview of recent developments on carbon-based nanomaterials with a focus on sustainable and clean energy applications. With chapters written by the leading academicians and researchers working in the field, the volume explores state-of-the-art developments using both commercially available and emerging materials and their potential applications for energy storage and energy harvesting.

Biotechnology of Microbial Enzymes: Production, Biocatalysis, and Industrial Applications, Second Edition provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions. The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last three decades have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book. This fully revised, second edition is updated to address the latest research developments and applications in the field, from microbial enzymes recently applied in drug discovery to penicillin biosynthetic enzymes and penicillin acylase, xylose reductase, and microbial enzymes used in antitubercular drug design. Across the chapters, the use of microbial enzymes in sustainable development and production processes is fully considered, with recent successes and ongoing challenges highlighted. - Explores advances in microbial enzymes from basic science through application in multiple industry sectors - Includes up-to-date discussions of metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and more - Provides a holistic approach to the research of microbial enzymes and their use in sustainable processes and innovation - Features all new chapters discussing microbial enzyme classes of growing interest, as well as enzymes recently applied in drug discovery and other applications

The book provides a detailed state-of-the-art overview of inorganic chemistry applied to medicinal chemistry and biology. It covers the newly emerging field of metals in medicine and the future of medicinal inorganic chemistry. Further it includes metal based medicines used in alternative systems of Ayurveda as well as Tibetan Zuotai to make it a holistic approach. It is an essential reading for every researcher and student in medicinal and bioinorganic chemistry.

Bioelectrochemistry is a fast growing field linking together electrochemistry, biochemistry, medicinal chemistry and analytical chemistry. The current book outlines the recent progress in the area and the applications in biological materials design and bioenergy, covering in particular biosensors, bioelectronic devices, biofuel cells, biodegradable batteries and biomolecule-based computing.

Cytokine Storm Syndromes, including HLH and MAS, are frequently fatal disorders, particularly if not recognized early and treated during presentation. The genetics of Cytokine Storm Syndromes are being defined with many of the risk alleles giving rise to mutations in the perforin-mediated cytolytic pathway used by CD8 cytotoxic T cells and natural killer cells. These are being studied using murine models. Up to 10% of the general population may carry risk alleles for developing Cytokine Storm Syndromes, and Cytokine Storm Syndromes are being increasingly recognized around the world in pediatric and adult hospitals. A variety of infectious, rheumatic, and oncologic triggers are commonly associated with Cytokine Storm Syndromes, but understanding this disorder is critical for all researchers and physicians to ensure timely and appropriate therapy. This textbook, the first of its kind, addresses all aspects of the disorder – from genetics, pathophysiology, and ongoing research,to clinical presentations, risk factors, and treatment.

In the last couple of decades, research in the area of tissue engineering has witnessed tremendous progress. The focus has been on replacing or facilitating the regeneration of damaged or diseased cell, tissue or organs by applying a biomaterial support system, and a combination of cells and bioactive molecules. In addition new smart materials have been developed which provide opportunities to fabricate, characterize and utilize materials systematically to control cell behaviours and tissue formation by biomimetic topography that closely replicate the natural extracellular matrix. Following on from Smart Materials for Tissue Engineering: Fundamental Principles, this book comprehensively covers the different uses of smart materials in tissues engineering, providing a valuable resource for biochemists, materials scientists and biomedical engineers working in industry and academia.

This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research.This book covers:The last one-hundred years of innovative research done in enzyme immobilizationRecent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its propertiesThe physiological and industrial significance of enzymesfrom plants and the implementation of immobilized enzymes in the treatment of waste water and polluted airBiomedical and bioanalytical applications of immobilized enzymes

These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant. - Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field - Covers conventional and new hydrogen sulfide detection methods - Covers the pharmacological activity of hydrogen sulfide donors - Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation

These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant. - Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field - Covers conventional and new hydrogen sulfide detection methods - Covers the pharmacological activity of hydrogen sulfide donors - Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation

Global warming, pollution, food and water shortage, cyberspace insecurity, over-population, land erosion, and an overburdened health care system are major issues facing the human race and our planet. These challenges have presented a mandate to develop “natural” or “green” technologies using nature and the living system as a guide to rationally design processes, devices, and systems. This approach has given rise to a new paradigm, one in which innovation goes hand-in-hand with less waste, less pollution, and less invasiveness to life on earth. Bioinspiration has also led to the development of technologies that mimic the hierarchical complexity of biological systems, leading to novel highly efficient, more reliable multifunctional materials, devices, and systems that can perform multiple tasks at one time. This multi-volume handbook focuses on the application of biomimetics and bioinspiration in medicine and engineering to produce miniaturized multi-functional materials, devices, and systems to perform complex tasks. Our understanding of complex biological systems at different length scales has increased dramatically as our ability to observe nature has expanded from macro to molecular scale, leading to the rational biologically-driven design to find solution to technological problems in medicine and engineering.The following three-volume set covers the fields of bioinspired materials, electromechanical systems developed from concepts inspired by nature, and tissue models respectively.The first volume focuses on the rational design of nano- and micro-structured hierarchical materials inspired by the relevant characteristics in living systems, such as the self-cleaning ability of lotus leaves and cicadas'wings; the superior walking ability of water striders; the anti-fogging function of mosquitoes'eyes; the water-collecting ability of Namib Desert Beetles and spider silk; the high adhesivity of geckos'feet and rose petals; the high adhesivity of mussels in wet aquatic environments; the anisotropic wetting of butterflies'wings; the anti-reflection capabilities of cicadas'wings; the self-cleaning functionality of fish scales; shape anisotropy of intracellular particles; the dielectric properties of muscles; the light spectral characteristics of plant leaves; the regeneration and self-healing ability of earthworms; the self-repairing ability of lotus leaves; the broadband reflectivity of moths'eyes; the multivalent binding, self-assembly and responsiveness of cellular systems; the biomineral formation in bacteria, plants, invertebrates, and vertebrates; the multi-layer structure of skin; the organization of tissue fibers; DNA structures with metal-mediated artificial base pairs; and the anisotropic microstructure of jellyfish mesogloea. In this volume, sensor and microfluidic technologies combined with surface patterning are explored for the diagnosis and monitoring of diseases. The high throughput combinatorial testing of biomaterials in regenerative medicine is also covered.The second volume presents nature-oriented studies and developments in the field of electromechanical devices and systems. These include actuators and robots based on the movement of muscles, algal antenna and photoreception; the non-imaging light sensing system of sea stars; the optical system of insect ocellus; smart nanochannels and pumps in cell membranes; neuromuscular and sensory devices that mimic the architecture of peripheral nervous system; olfaction-based odor sensing; cilia-mimetic microfluidic systems; the infrared sensory system of pyrophilous insects; ecologically inspired multizone temperature control systems; cochlea and surface acoustic wave resonators; crickets'cercal system and flow sensing abilities; locusts'wings and flapping micro air vehicles; the visual motion sensing of flying insects; hearing aid devices based on the human cochlea; the geometric perception of tortoises and pigeons; the organic matter sensing capability of ca

Orphan drugs are designated drug substances that are intended to treat rare or ‘orphan'diseases. More than 7000 rare diseases are known that collectively affect some 6-7% of the developed world's population; however, individually, any single, rare disease may only affect a handful of people making them commercially unattractive for the biopharmaceutical industry to target. Ground breaking legislation, starting with the Orphan Drug Act that was passed in the US in 1983 to provide financial incentives for companies to develop orphan drugs, has sparked ever increasing interest from biopharmaceutical companies to tackle rare diseases. These developments have made rare diseases, and the orphan drugs that treat them, sufficiently attractive to pharmaceutical development and many pharmaceutical companies now have research units dedicated to this area of research. It is therefore timely to review the area of orphan drugs and some of the basic science, drug discovery and regulatory factors that underpin this important, and growing, area of biomedical research. Written by a combination of academic and industry experts working in the field, this text brings together expert authors in the regulatory, drug development, genetics, biochemistry, patient advocacy group, medicinal chemistry and commercial domains to create a unique and timely reference for all biomedical researchers interested in finding out more about orphan drugs and the rare diseases they treat. Providing an up-to-date monograph, this book covers the basic science, drug discovery and regulatory elements behind orphan drugs and will appeal to medicinal and pharmaceutical chemists, biochemists and anyone working within the fields of rare disease research and drug development or pharmaceuticals in industry or academia.

It was just about ten years ago that platelet membrane glycoproteins were first charac­ terized and their abnormalities in congenital bleeding disorders first recognized. Dur­ ing this decade there has been a remarkable growth in our understanding of the structure and membrane organization of the platelet surface glycoproteins, their in­ teractions with external ligands during the process of hemostasis, and their defects causing hemorrhagic disease. These studies have advanced the knowledge of platelet involvement in hemostasis from a cellular to a molecular level, and they have provided a model for contact interactions among other cell types. This seemed a proper time to ask those who contributed major observations and insights during these past years to review their progress and to assess the state of our present knowledge. We have planned this volume to begin with the biochemistry of platelet membrane glycoproteins themselves and proceed through their involvement in platelet function to the final considerations of the platelet's role in maintaining the integrity of the vascular system. Our aim was an integrated presentation on the blood platelet from the perspective of its highly specialized and reactive cell surface. James N. George Alan T. Nurden David R. Phillips vii Contents I. INTRODUCTION 1. Plasma Membrane Receptors and Platelet Response Ernst F. Wscher 1. Introduction.................................................... 3 2. Platelet Receptor Functions.............................. 4.......... 2. 1. Receptors Involved in Platelet Adhesion and in Cell-Cell Contact... 5 2. 2. Receptors Involved in Platelet Activation................... 6.... 3.Conclusion..................................................... 8 References..................................................... 8 II.

Steroid hormones secreted from peripheral reproductive endocrine tissues regulate many important physiological functions not only during development, but also into adulthood and play a key role in the growth, differentiation and function of reproductive tissues. In this book, the authors present current research in the study of the biosynthesis, functions and health implications of steroids. Topics include cytotoxic steroids and derivatives in the development of anticancer drugs; identifying steroid hormones in biological and environmental samples; steroid receptors involvement in the pathogenesis of abnormal ovarian follicular cysts; estrogen treatment after menopause to protect against dementia and cognitive decline; the evolving role of steroids in immunosuppression and transplantation; nasal steroids in adenoidal hypertrophy; and cytogenetic damage following anabolic steroids exposure.

Over the last decades cell biology and biological chemistry have increasingly turned their attention to the space between cells and revealed an elaborate network of macromolecules essential for structural support, cell adhesion and signaling. This comprehensive handbook of the extracellular matrix will give an overview of the current state of knowledge of matrix components (structure and function), their role in heath and disease (matrix pathobiology) and new aspects related to pharmacological targeting.It will provide an introduction to the extracellular matrix and detailed sections and chapters on: Importance of extracellular matrix in health and disease Matrix proteoglycans (aggrecan, versican, perlecan, SLRPs, syndecans, glypicans, serglycin) Matrix proteinases (remodeling, would healing, regulatory roles in health and disease, metalloproteinases, cystein proteases, plasmin and plasminogen activator system) Glycobiology (hyaluronan and sulfated glycosaminoglycans in cancer, inflammation and metabolic control) Collagens (supramolecular assembly, proteins binding collagen, scaffolds, bacterial and mutated collagens, procollagen proteinases) Cell surface receptors (integrins, syndecans, mechanical strain and TGFb, CD44 and DDR). Biotechnological and pharmacological outlook (matrix regulation by growth factors, hyaluronidases, pathobiology, disease targeting, delivery systems, EMT and proteomics).'The book Extracellular Matrix: Pathobiology and Signaling provides a comprehensive and up to date collection of very relevant topics for understanding the various facets of extracellular matrix and its interactions with cells in normal tissue as well as in disease. It represents the current front-line and will serve as a reference for extracellular matrix and posttranslational modifications.'Dick Heinegård, Department of Clinical Sciences Lund, Section Rheumatology, Lund University, Sweden

Research on thrombin structure and function has progressed significantly over the past three decades. We are continually discovering new functions f()r this enzyme in biology. Yet, until quite recently, a full, detailed, three­ dimensional picture of its structure was difficult to attain. We believe that this text represents a turning point and, more appropriately, a new start­ ing point for thrombin studies. Our goal for this text is to present a thorough and rounded-out coverage of thrombin chemistry and biochem­ istry in order to provide the biochemist and physiologist with an excellent desk reference on almost any thrombin-related problem. This volume is organized into three general thrombin topic areas: Structure, Biochemistry, and Physiology. In Part 1, Structure, we open with the complete three-dimensional x-ray structures of two inhibited human thrombin complexes, one of which is the thrombin-hirudin com­ plex. These complexes are also addressed in the chapter on structural studies in solution, which include NMR, ESR, and fluorescence. Part 2, Biochemistry, includes chapters on synthetic thrombin inhibitors, protein inhibitors (e.g., antithrombin III, hirudin), and thrombin interactions with factor XIII. Part 3, Physiology, covers such topics as chemotactic activities, interactions with cell surfaces, and the vascular endothelium.

Proteomics is the large-scale study of proteins, particularly their structures and functions. Proteins are vital parts of living organisms, as they are the main components of the physiological metabolic pathways of cells. The proteome is the entire complement of proteins, including the modifications made to a particular set of proteins, produced by an organism or system. This book presents and discusses various topical data on proteomics, including: proteomic technologies used in celiac disease, protein location in plant proteomics, the prediction of protein-protein interactions (PPIs) using proteomics, proteomic studies of mild cognitive impairment, among others

Since its inception in 1945, this serial has provided critical articles written by research specialists that integrate industrial, analytical, and technological aspects of biochemistry, organic chemistry, and instrumentation methodology in the study of carbohydrates. - Features contributions from leading authorities and industry experts - Informs and updates on all the latest developments in the field

Understanding how angiogenesis'works'and how to control it will have massive implications on the management, treatments, and ultimately the prevention of many common (and not so common) diseases. Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over angiogenesis. Diseases that are angiogenesis-dependent result when blood vessels either grow excessively or insufficiently. - Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition - Provides the'builder's manual'for essential techniques--a one-stop shop that eliminates needless searching among untested techniques - Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular Biology™ series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

Programmed cell death (PCD) has become a topic of widespread interest and experimentation over the past decade. Written by experts in the field,Apoptosis: Pharmacological Implications and Therapeutic Opportunities concentrates on presenting an overview of PCD pathways as they are currently understood, and strives to identify important unanswered questions as well as other therapeutic possibilities suggested by recent biochemical discoveries. Understanding the biochemical pathways that participate in the cell death process has become an important goal in developmental biology, neurobiology, cardiology, and infectious disease research. The control of apoptosis has also become a major area of investigation in the field of cancer biology.Apoptosis: Pharmacological Implications and Therapeutic Opportunities contains an overview for those with a general interest in apoptosis, and provides discussions of sufficient depth to be useful for specialists in the field.

Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.

Since its inception in 1945, this serial has provided critical articles by research specialists in the industrial, analytical, and technological aspects of biochemistry, organic chemistry, and instrumentation methodology. The articles provide a definitive interpretation of the current status and future trends in carbohydrate chemistry and biochemistry.

Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.Key Features• This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume• Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics• Provides useful, detailed, easy-to-follow, step-by-step protocols• Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types• Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates• Cites over more than references keyed to concepts covered in the book• Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications• Suggests sources for all key reagents