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This fully updated and expanded second edition of a highly popular text book focuses on the structure and mechanism in carbohydrate chemistry and biochemistry. Carbohydrates play important roles in biological systems as energy sources, as structural materials, and as informational structures (when they are often attached to proteins or lipids). Their chemical reactivity and conformational behaviour is governed by mechanistic and stereochemical rules, which apply as much to enzymic as to non-enzymic reactivity. The same principles of reactivity and conformation govern changes brought about in the process industries, such as pulp, paper and food. Extensively referenced with citations and a detailed index, the book contains everything the reader needs to know to start a carbohydrate research project with one of the real strengths being the treatment and integration of the important physical-chemical principles and methods (though lead references only are given to the finer points of carbohydrate synthesis). The book is suitable for both researchers who are new to the subject and those more established as well as a readership from diverse backgrounds and interests, including chemists, biochemists, food scientists and technologists involved with the processing of polysaccharides in the paper, textile, cosmetics, biofuels and other industries.

This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.

Biotechnology refers to the use or manipulation of an organism or parts of an organism. While the early applications were certainly simpler (though still relevant), modern plant biotechnology is primarily associated with molecular biology, cloning and genetic engineering. Over the last 50 years, several key discoveries have revolutionized the biological sciences and enabled the rapid growth of the biotechnology industry.This book gathers handpicked articles presented by national and international scientists at the International Conference on Biotechnology and Biological Sciences, BIOSPECTRUM 2017. It highlights the works of researchers and students in India and abroad on plant biotechnology and its applications in addressing various agricultural and food production-related issues.The respective papers explore a range of advances in plant biotechnology, e.g.: the cytotoxic potential of Moringaoleifera lam; the use of the entomo-pathogenic fungi Cordyceps sp. as unique and valuable sources of bioactive compounds; and strain improvement strategies for Cordyceps sp. In addition, they discuss the use of low-cost blue green algal biofertilizer comprising four blue green algal strains in rice fields; and the use of lignocellulosic materials as potential renewable energy resources for the production of fuels.This book will be extremely useful for researchers and students of biotechnology and plant science, providing an essential update on the latest findings and trends.

This proceedings volume contains a selection of invited and contributed papers of the 9th International Workshop on Sulfur Metabolism in Plants, which was hosted by Heinz Rennenberg, Albert-Ludwigs-University Freiburg and was held at Schloss Reinach, Freiburg-Munzigen, Germany from April 14-17, 2014. The focus of this workshop was on molecular physiology and ecophysiology of sulfur in plants and the content of this volume presents an overview on the current research developments in this field.

This book brings together the molecular aspects of the pathogenesis of entomopathogenic fungi, various aspects of mycoinsecticide development, and regulatory aspects to highlight the immense contribution of the agricultural sector. The chapters address aspects of entomopathogenic fungi, including host-pathogen interactions (susceptibility and resistance), fungus-insect and fungus-fungus dual interactions, phylogeny and taxonomy, biochemistry, and molecular basis of enteropathogenesis, market potential of entomopathogens, regulatory aspects, bioprospecting of fungi, fungi as crop bodyguards and in disease suppression, and consortia for the control of insect pests and pathogens in single crop systems. Written by experts in academia and industry from Algeria, Brazil, Hungary, India, Italy, Malaysia, Oman, Pakistan, and the United States, the book overviews entomopathogenic fungi used as biological control agents against insect pests and other arthropods, their mode of action, pathogenesis, bioactive metabolites, mycotoxins involved, their mass production, limitations, and agricultural- and environment-related issues. It also includes developing various biopesticide products, with special references to formulations and prospects. In agriculture, crop protection and production depend on insect pests controlled by entomopathogenic fungi. Understanding the pathogenicity, mechanism of action, formulations, and applications of a wide array of entomopathogenic fungi as biopesticides is a valid eco-friendly approach. The book provides advances in pathogenicity, biochemistry, virulence, and interaction that facilitate the application of Beauveria, Metarhizium, and other entomopathogens as potential biopesticides against major insect pests. Developments in bioactive compounds, secondary metabolites, enzymes, and toxins of entomopathogenic fungi have facilitated precise applications and management to target insect pests. Further advances in molecular aspects, the formulation of nanobiopesticides, and biofabricated technology paved the way for insect pest control. This book will be a helpful resource for students, teachers, researchers, scientists, and professionals in the industry working on biological sciences, applied entomology, mycology, zoology, forestry, biochemistry, molecular biology, and nanotechnology.

This book focuses on the fundamental processes involved in calcium signaling through fractional mathematical modeling. The intended reader of the book is mathematically proficient students of cell biology, applied mathematics and mathematical biology who are interested in learning about modeling in cell biology. The book will inspire the graduate students and researchers to collaborate with experimentalists while studying the direction needed to explore the modeling literature. Organized into four chapters, the book introduces to fractional calculus and devoted to understanding the receptor activation by the ligand, that is Thrombin via fractional model incorporating the Caputo-Fabrizio fractional operator. It studies the calcium profile in red blood cells with the help of the advection-diffusion equation. The book further studies the process of buffering, aiding to the lowering of the calcium concentration in the cytosol, which rises due to calcium influx from extracellular space and intracellular storage. A mathematical model is studied to characterize the anomalous subdiffusion of cytosolic calcium.

Biochemistry is concerned with the chemical processes that occur within living organisms and microorganisms. There have been a number of publications focusing on biochemistry and its use for understanding biochemical and molecular mechanisms, with the majority of the literature focusing on bench scale items. To date there has not been a comprehensive work focusing on the techno-economic and industrial aspects of biochemistry from the microeconomic and pilot scales. This text covers current innovations and advances in plant biochemistry, animal biochemistry, microbial biochemistry and medicinal biochemistry plus potential uses of proteomics, genomics, recombinant DNA technology and protein application. Recent Advances in Industrial Biochemistry focuses on methods for recombinant proteins production and purification plus metabolic engineering and other source technologies from the industrial viewpoint, providing comprehensive, up-to-date information and evidence on contemporary development in the field of industrial biochemistry. The major focus of this book is the key issues, opportunities, approaches, advancements, products, innovations and technologies in current biochemistry from micro scale to production at pilot scale. Chapters highlight the many potential commercial prospects in various industries from food to to pharmaceuticals to bioenergy, providing a valuable and unique single resource for researchers.

This book overviews ongoing and upcoming clinical applications of basic immunology. Recent advances in our knowledge of immunology coupled with new technologies have aided in the development of efficient cancer immunotherapy, as well as the control of emerging microorganisms such as SARS-CoV-2. However, knowledge of basic immunology has not been fully utilized even after the discoveries of immune checkpoint inhibition for cancer immunotherapy and the development of mRNA vaccination against SARS-CoV-2. There is still room for improving the clinical application of basic immunology. The book summarizes the achievements in clinical applications of basic immunology and highlights what can be further extended to make immunology a more practical human science. Basic immunology and its clinical applications are two wheels of the same cart in the immunology field, which aids in the development of more efficient cancer immunotherapy and rapid control of infectious diseases against microorganisms, including new viruses and classical toxoplasmosis. The exploration of ongoing and upcoming applications of basic immunology in this book makes it a useful resource for immunologists, physicians, molecular and genome biologists, bioinformaticians, and students in these fields.

Molecular hydrogen (H2) has emerged as a promising therapeutic and preventive medical gas. Hydrogen gas has garnered significant attention in recent years due to its remarkable antioxidant and anti-inflammatory properties. H2 exhibits exceptional pharmacokinetics, swiftly traversing cellular biomembranes to access subcellular organelles indirectly regulating hormones and cytokines through various signal transduction pathways. H2 has the potential to address a wide range of issues, including clinical medical treatment in the areas of healthcare: myocardial ischemia, heart failure, cardiac arrest, metabolic syndrome, advanced-stage cancer, inflammatory diseases, Alzheimer's dementia, aging-related disorders, sports activities, and even beauty, agriculture, etc.Selected world-known authors in the field provide readers with a comprehensive overview of molecular hydrogen's remarkable effects and its physical, chemical, and therapeutic properties and potential in biomedical applications. This contributed volume fills the current gap in information and is intended for everyone who wants to be better informed about the wide possibilities of using molecular hydrogen. However, it is mainly intended for use by medical students, physiologists, pharmacologists, physicians, and other healthcare personnel who can use molecular hydrogen to improve the health of everyone who needs it.

This textbook introduces the clinical applications of biomolecules in disease diagnosis, covering a range of topics in biochemistry. The book discusses carbohydrate and protein metabolism abnormalities, lipid digestion and absorption defects, disorders of purine and pyrimidine metabolism, and the chemistry and biological roles of water- and fat-soluble vitamins. It also covers the roles of different macroelements and essential trace elements in physiological processes and the prevention of chronic diseases. The final chapter focuses on recent developments in our understanding of the role of enzymes in disease development and diagnosis. The book chapter also explores the causes and effects of free radicals in the body, including oxidative stress and diseases such as cardiovascular and neurodegenerative diseases. Finally, the chapter discusses cancer development, causes, diagnosis methods, biomarkers, and current treatments such as targeted drug therapy, chemotherapy, and radiation therapy. This book is a useful resource for undergraduate and postgraduate students, researchers and practitioners in biochemistry, medicine, and health sciences.

The second edition of this book presents the role of osmolytes in human health and diseases. Some of the chapters deal about the possibility of the use of osmolytes as diagnostic biomarkers and potential drug design for neurodegenerative and other human diseases. Other chapters also include reviews on the role of osmolytes in cancer, metastasis, infectious diseases, metabolic disorders, immunological disorders, and tissue regeneration. Importantly, the book also contain recent updates on the role of naturally occurring osmolytes in protein folding pathway, protein stability, and their underlying mechanisms. The book also covers the aspects that osmolytes could promote conformational alterations of transcription factors that favor metastatic behavior. Potential of the osmolytes in the various process of vaccine development, including enhancing the efficacy, production, and purification steps are also succintly described. Towards the end, the book also elucidates the use of specific molecules for the prevention of toxic gain of functions and restoration of function to disease-causing mutant protein. This book is an invaluable asset for the researchers especially working in osmolyte biology and scientists involved in basic and clinical research particularly neurodegeneration, diabetes, cancer, and metabolic disorders.

This book focuses on the various invasive and non-invasive techniques which can be used for the characterization of starch macromolecules along with, the various types of physical, chemical, and enzymatic modifications of starch to enhance its usage in the food industry. It discusses various biophysical techniques, including scanning electron microscopy, Fourier transforms infrared spectroscopy, X-ray diffraction, Raman scattering, and second harmonic generation microscopy for the understanding of the Physico-chemical properties of starch. The book also sheds light on the visual, rheological characterization of different types of starches that are responsible for altered digestibility. The chapters also cover the applications of starch in food industries, non-food industries, pharmaceuticals, drug delivery systems, and green flexible electronics. Towards the end, the book reviews the chemical, physical, and enzymatic modifications of the starch for improving its properties and applications. This book provides a valuable reference for students and researchers in the field of food science and technology, food science, and nutrition.

This book offers a comprehensive perspective of herbal medicine phytochemistry and explores the application of plant extracts as bioactive compounds in disease prevention and treatment in modern or traditional medicine. The book starts with an introduction to the history and value of herbal medicine, followed by 3 parts covering the main phytochemical components and metabolites in herbal medicine, different uses and practices in herbal medicine, including a region-wise analysis of methods and practices and an overview of regulations and policies for herbal medicinal practitioners, and the advances and challenges in quality assessment of herbal medicine. Plants generally have the tendency to bioaccumulate trace metals from the environment and they are easily contaminated by microorganisms from water sources and poor hygiene practices of the herbalist. Quality assessment and assurance is, thus, a pertinent challenge in herbal medicine practice (i.e., in remedy formulation and application), and this book offers an authoritative perspective on this topic, covering aspects such as quality control strategies, preparation techniques, chemical quantification in phytomedicine, and the efficacy and safety of herbal remedies. Moreover, in this book, readers will find valuable insights into the latest trends and developments in the field, and a critical review of the application of medicinal plants to treat cardiovascular, digestive, respiratory neurological and reproductive diseases. Particular attention is given to the advances and trends in the field, and readers will learn about the latest biotechnological approaches, the use of nanotechnology in herbal medicine, metabolomic analysis of medicinal plants, big data application in herbal medicine, and the value of herbal medicine towards sustainability. Given its breadth, this book is aimed at researchers, academics, practitioners and professionals working in the fields of natural, life, health, clinical, and biomedical sciences, and interested in herbal remedies, pharmacology, pharmacognosy, human nutrition and dietetics, plant biology, and biotechnology/microbiology.

This book reviews the potential of metallodrugs against different cancer. It summarizes the classification of metal-based anti-cancer drugs, their plausible biochemical and mechanistic pathways, combining drug strategies for hitting multiple therapeutic targets at the intracellular level, and advanced drug delivery strategies. The book covers the metallodrugs for the efficacious treatment of diverse cancerous strains and recent advances in drug delivery strategies that are used for developing these metal-based therapeutics as potent anticancer agents in vitro and in vivo. The book also covers different biophysical and analytical techniques for studying metal-ligand and metal-macromolecular interactions. The book further presents the recent examples of metallomics studies on the different types of cell death induced by metal-based anticancer drugs, especially on the three major forms of programmed cell death (PCD) in mammalian cells: apoptosis, autophagy, and regulated necrosis, alsocalled necroptosis. Lastly, the book explores the modulation of reactive oxygen species (ROS) by metallodrugs.

Modern research in biology increasingly relies on multiple techniques for describing structures and mechanisms. This book provides an overview of the contemporary integrated biology approaches for solving structures and understanding mechanisms of complex biological systems. It includes several methodology chapters discussing the current developments in the areas of cryo-electron microscopy (EM) and cryo-electron tomography (ET), computational biophysics, solution NMR spectroscopy, solid-state NMR spectroscopy and dynamic nuclear polarization (DNP), electron paramagnetic resonance (EPR), (photo-)chemically induced dynamic nuclear polarization (CIDNP), X-ray crystallography and small-angle X-ray and neutron scattering (SAXS/SANS). Several subsequent chapters demonstrate how these methods are used in synergy to address problems at the forefront of structural biology, with particular emphasis on examples where individual techniques are insufficient. Examples of biological systems include membrane proteins, viral protein assemblies, cytoskeleton protein assemblies, photosynthetic reaction centers, large enzyme complexes and whole cells. The book is targeted to both the current practitioners of structural biology and scientists who are interested in entering the fields of structural biology or biophysical chemistry.

This book provides new knowledge about the nutrition and metabolism, as well as the roles of nutrients in the immunity, health, and management of companion animals (dogs and cats). The domestic dog (facultative carnivore) and the domestic cat (obligate carnivore) have evolved differentially in their digestive tract, nutrition, metabolism, chemical sensing, and feeding behavior. These animals have been human companions for at least 12,000 and 9,000 years, respectively, and continue to contribute to the mental health and well-being of children, adolescents, and adults. Both dogs and cats have become increasingly popular in many countries and worldwide over the past decades. Comprehensive update about how the animals utilize dietary nutrients for optimum growth, development, and health is beneficial for their owners, as well as students and researchers. Chapter 4: Characteristics of Nutrition and Metabolism in Dogs and Cats is available open access under a Creative Commons Attribution 4.0 International License

This textbook is for students studying medicine and other biosciences. Understanding biochemistry requires basic understanding of organic chemistry. The main purpose of this book is, therefore, to help students to understand biomolecule-related organic chemistry. Fundamental theories such as the molecular orbital method, thermodynamic law, frontier orbital theory, and molecular interactions, which have not been covered in basic organic chemistry textbooks, are.

This book helps readers discover the forefront of personalized medicine on clinical metabolomics and its applications in genetic diseases. This comprehensive guide offers a functional relationship map between cell components and genetic variations in various diseases, providing insights that can be applied to personalized medicine. The book covers the latest developments in metabolomics for health, with practical guidance for clinical experts looking to advance their laboratory techniques and career. The metabolomics profile is a powerful tool that has revolutionized our understanding of the relationship between genetics, clinical readouts, and disease outcomes. By integrating metabolomics with genomics and clinical phenotypes, the authors have developed diagnostic and prediction models that have vastly improved patient outcomes and deepened the understanding of disease mechanisms. This model has been successfully applied in various conditions, including inborn errors of metabolism, primary immunodeficiency, and endocrine disorders. However, integrating metabolomics with other omics datasets and clinical phenotypes requires careful study design, analytical tools, and data analysis and interpretation. This groundbreaking new book provides essential guidance for researchers, students, and professionals looking to leverage metabolomics in their own work, including biochemical and clinical geneticists, pharmacogenomics and pharmacometabolomics experts, pharmaceutics and diagnostic researchers, medical scientists, clinical dietitians, metabolic engineers, clinical chemists, and personalized medicine specialists.

This book presents the latest cutting-edge research on phase separation. It discusses the benefits and risks of phase separation for living cells from the perspectives of physics, chemistry, biology, and medicine.Phase separation is a physico-chemical process that induces a single solution of solvent and intrinsically disordered proteins (IDPs) to separate into two phases, one phase containing only solvent and the other containing IDPs in the solvent. A key molecule in phase separation is the intrinsically disordered region (IDR) proteins (IDPs), mostly comprised of RNA-binding proteins (RBPs). One of the major roles of phase separation is to generate condensates, membrane-less organelles including stress granule, Cajal body, and nucleolus.Biological actions of phase separation in various aspects of science have received increasing attention in recent years. The book consists of four parts; Part I on physics and chemistry includes topics on structural biology of RBP FUS/TLS and chaperone for phase separation, computational approach of phase separation, and chemistry of G-quadruplex of DNA and RNA. Part II on molecular biology presents molecular mechanism of IDR sequence phase separation and potential cellular function of these labile fibers, formation of membrane-less organelles, and roles of noncoding RNAs in phase separation. Part III on biology covers topics from nuclear pore complex, developmental biology regarding force-dependent remodeling, and neurobiology and higher order brain functions. Finally, Part IV on medicine presents condensates and cancer therapy, pathogenesis of neurodegenerative diseases based on IDPs, and responses of microglia to amyloid. The last chapter highlights the latest topic on how the SARS-Cov-2 virus takes advantage of phase separation for its infections.This book brings together the studies of phase separation in each area in a single volume. The comprehensive approach provides new insights to the research andwill benefit the readers by responding to the emerging needs for understanding phase separation.

This book presents a major research program on epigenetic mechanisms of cell programming and reprogramming. The program was launched by the National Natural Science Foundation of China during the 11th Five-Year Plan period, which was started in October 2008 and concluded at the end of 2016. It first summarizes the epigenetic research plans and the current situation of epigenetic research in China and the international frontier. With the application of interdisciplinary research methods, the book discusses the development trends of epigenetic research, such as mechanisms of establishment and maintenance of epigenetic information and epigenetic regulation on reproduction and development. It also presents a variety of research achievements. For example, the discovery of new epigenetic regulators and chromatin remodulaters and their biological functions and mechanisms are highlighted. Several regulation mechanisms of cell differentiation and trans-differentiation are discussed, especially the discovery of the key factors to promote the trans-differentiation of somatic cells to hepatocytes. In addition, this book puts forward some key academic fields with increasing importance, such as biochemistry, cell biology, and clinical medicine. Given its scope, the book is of interest to researchers who work in cell biology, biochemistry, structural biology, bioinformatics, clinical medicine, and other related fields.

This book provides a comprehensive and authoritative review of cyanobacteria and their applications as solar cell factories. Cyanobacteria are able to perform oxygenic photosynthesis and they are utilized in many different bioprocesses. The book covers two major aspects of a cyano-based bioprocess: the biological whole-cell catalyst and the technical environment in which the catalyst is applied. In the biocatalyst itself electron and carbon flow play an essential role for the performance of the cell and need to be tackled likewise for successful biocatalyst development. In the first chapters of this volume, cyanobacterial biotechnology and the fundamentals of cyanobacterial bioenergetics are introduced followed by an overview on tools and strategies for cyanobacteria engineering. Further on, examples of applications, engineering and production of different industrially relevant compounds in these organisms are provided, and finally process technology specific for cyanobacteria is covered. In this book, particular attention is given to topics such as cyanobacterial bioenergetics, metabolic engineering design strategies, utilizing cyanobacteria in biophotovoltaics, and production of pigments as well as photobiohydrogen in cyanobacteria, among others. This book will provide interested students and researchers in the area of photo-biotechnology with a deeper understanding of the cyanobacterial cell-factory, including the latest innovations and persistent challenges in the field.

The book is aimed at providing an exposure to some important topics which are generally not covered adequately in formal courses in biotechnology. It informs the readers about: How micro-fluidics are proving useful in enzyme kinetics. Chemi-proteomics; combinatorial chemistry and high-throughput screening in the context of drug discovery. How enzymes can be used with gaseous substrates? How to source more robust enzymes from marine resources for diverse applications? Why some nano-materials can be chiral? Synthesis of diverse quantum dots as powerful fluorescent probes in biology. How basics of surface chemistry and immunology are vital in dealing with endemics/pandemics like Covid-19.

Biochemistry is the study of the structure and functions of biological macromolecules such as nucleic acids, proteins, carbohydrates and lipids. The book is organized in five chapters which covers the basic concepts and fascinating chemistry of biomolecules. It also exposes students to different metabolic pathways and concept of energy in biological system, and provides valuable material for the students of Chemistry, Biochemistry, Biotechnology and Bioscience.

This thesis addresses deep questions that cut to the physical and informational essence of central chemical quantities such as transition paths and reaction mechanisms and proposes fundamental new connections between transition-path theory, linear-response theory, nonequilibrium thermodynamics, and information theory. The author investigates slow, energetically efficient driving protocols that drive a system between conformations corresponding to endpoints of a reaction, aiming to find connections between principles of efficient driving and the spontaneous transition mechanism in the absence of driving. First, an alternative perspective of transition-path theory is developed that unifies it with stochastic thermodynamics to describe flows of entropy, energy, and information during the reaction. This also provides an optimization criterion for selecting collective variables. Next, protocols are designed which invert the magnetization of a 3×3 Ising model with minimal energetic cost,and it is determined that using multiple control parameters allows the system to be driven along a fast-relaxing pathway between reaction endpoints. Finally, the author compares these protocols with the spontaneous transition mechanism for magnetization inversion in the same Ising model, finding that designed protocols capture general features of the spontaneous mechanism and energetics given the constraints on the control parameters. This work represents a major step forward in our understanding of rare events and provides a basis for investigating the connection between efficient protocols and spontaneous transition mechanisms which can be further probed in a wider variety of systems.

Cholesterol is an essential component of the plasma membrane. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), although a minor phospholipid, is the most abundant membrane phosphoinositide. Both lipids play key roles in a variety of cellular functions including as signalling molecules and major regulators of protein function. Studies on these important lipids have traditionally focused on the effect of each lipid individually. Accumulating evidence indicates, however, that these lipids may cross-regulate each other's levels. Furthermore, it is becoming evident that cholesterol and PI(4,5)P2 can act together to modulate protein function and biological processes. This book provides an overview of cellular functions and molecular mechanisms in which cholesterol and PI(4,5)P2 functions extend from parallel existence to crosstalk. It includes four sections. The first section introduces the reader to cholesterol and PI(4,5)P2. The second section demonstrates the mutual influence of these two critical lipids on their levels. The third section, divided into two parts, describes the co-modulation of protein function by cholesterol and PI(4,5)P2. The first part focuses on ion channels and the second - on lipid transfer proteins. The fourth section highlights other cellular processes at the intersection of cholesterol and PI(4,5)P2 involvement. Collectively, the book portrays the emerging relationship between cholesterol and PI(4,5)P2 in a broad array of biological systems and processes. The book will be of interest to a wide audience of research scientists with an interest in the biophysical properties of lipids and the physiological consequences of their presence in biological systems, as well as graduate students, postdoctoral trainees, basic and clinical researchers, and pharmaceutical scientists. Specifically, the content will be relevant to researchers in the fields of biochemistry, molecular biophysics, pharmacology, neurobiology, cardiovascular biology, among others.Provides a comprehensive overview of the current knowledge of the interplay between cholesterol and PI(4,5) P2Provides an overview of the emerging relationship between cholesterol and PI(4,5)P2 in biological systems and processesDiscusses cellular processes and molecular mechanisms where lipid functions extend from parallel existence to crosstalk

Biochemistry is the foundation for solving the complexity of life on earth. All living organisms on earth contains nucleic acids, lipids, carbohydrates, proteins, enzymes and hormones that are crucial for survival. Biochemistry plays essential role in understanding the mechanism, action and regulation of biomolecules in different metabolic pathways. Several analytical techniques are reported to characterize, analyse and quantify the biomolecules present in living organism. This book covers the diversity of biomolecules present in living organisms, their characterization techniques, clinical biochemistry and genetic information transfer process. This book has been designed to suit the knowledge and pursuit of the researcher and scholars and to empower them with various aspects of biochemistry so that they are updated with the information.

This book offers a comprehensive review of the latest developments in medicinal mushroom biochemical engineering and biotechnology, and it also analyses the circular economy of mushroom bioproduction. Divided into 13 chapters, the book begins with a historical perspective of medicinal mushrooms, followed by authoritative chapters that explore the farming of medicinal mushrooms and bioeconomy, as well as the limitations of using medicinal mushrooms to produce metabolites. Subsequent chapters cover topics such as solid-state and submerged cultivation of medicinal mushroom mycelia in bioreactors, pilot and industrial bioreactor cultivation experiences, downstream processing of medicinal mushroom products, and biochemistry of medicinal mushroom bioactive compounds. Particular attention is given to the recent genetic engineering techniques applied in mushroom cultivation. The book closes with a chapter devoted to the health and clinical benefits of medicinal fungi, where readerswill find expert insights into the therapeutic implications of medicinal fungi. In this book, readers will find an authoritative perspective on the past, present and future of medicinal mushrooms, and will also learn about some recent clinical studies with isolates from these natural products. Given its breadth, this book will appeal to biotechnologists working in mushroom cultivation, as well as to professionals interested in traditional pharmacy and medicine.

This book outlines the use of supramolecules as different pharmaceutical drugs. Supramolecular chemistry in pharmaceutical sciences is quite a young and rapidly developing field. Supramolecular assemblies might offer an alternative for existing pharmaceutical formulations, as they facilitate the improvement of physicochemical and pharmacological properties i.e., higher bioavailability, better biocompatibility and drug-targeting, fewer multidrug-resistances. This book offers an overview of the recent advances in supramolecular structures and discusses the future aspects and challenges related to the development of these molecules, providing also a perspective on how to overcome these issues. Divided into 13 chapters contributed by experts in their field, the book provides a deeper understanding of intermolecular forces playing pivotal roles in mediating the interactions between chemical molecules and biological systems by focusing on different applications of supramolecularcompounds. In this book, readers will find valuable insights into the preparation of supramolecules and the latest research and development trends of supramolecules as anticancer drugs, including liquid-crystalline supramolecular assemblies, and as antimicrobial, antiviral, anti-inflammatory and cardiovascular drugs. Particular attention is given to the application of supramolecules in the fields of biomedicine, bioimaging, and vaccine development. Given its breadth, this book will appeal to a wide readership from researchers and students interested in these fields to professionals in the pharma industry.

This book aims to summarize the latest research progress of biomedical nanozymes in diagnostic and therapeutic related fields. In terms of diagnostics, it will cover in vitro diagnostics, live analysis, and some emerging bioanalysis. In terms of therapeutics, it will introduce antioxidant therapy, peroxidation therapy and emerging therapy respectively. Furthermore, the research results previously scattered in many journals and conferences worldwide are methodically edited and presented in a unified form. Based on the new ideas and developments presented in this book, it will be of interest to readers from different areas, such as chemistry, materials, nanoscience and nanotechnology, biomedical and clinical studies, etc.

This book presents a collection of chapters on Covid-19 topics, with focus on the metabolomics and diagnosis. The chapters report on diagnostic tests for detection of SARC-CoV-2 virus and related proteins as well as post-Covid omics. This book covers Fourier-Transform Infrared spectroscopy and spectromicroscopy applied to Covid-19 studies and Carbon Nanomaterials as Covid-19 Electrochemical sensors. Moreover, the reader will find content on 3D printing for virus diagnosis, as well as organ-on-a-chip models for viral infection proteomics interaction.

This book covers foundational topics in the emerging field of radiopharmaceutical therapy. It is divided into three sections: fundamentals, deeper dives, and special topics. In the first section, the authors examine the field from a bird's-eye view, covering topics including the history of radiopharmaceutical therapy, the radiobiology of radiopharmaceutical therapy, and the radiopharmaceutical chemistry of both metallic and non-metallic radionuclides. The second section provides a more in-depth look at specific radiotherapeutics. Chapters include broader discussions of the different platforms for radiopharmaceutical therapy as well as more focused case studies covering individual radiotherapeutics. The third and final section explores a number of areas for further study, including medical physics, artificial intelligence, in vivo pretargeting, theranostic imaging, and the regulatory review process for radiotherapeutics.This book is the first of its kind andis useful for a broad audience of scientists, researchers, physicians, and students across a range of fields, including biochemistry, cancer biology, nuclear medicine, radiology, and radiation oncology.

This contributed volume provides an overview of the recent advances in solid-state nanopore technology, featuring contributions by leading experts in the field. It discusses several aspects of solid-state nanopores, covering their fabrication as well as multiple biosensing applications. It successfully bridges the gap between various scientific and engineering disciplines and highlights the progress made in this area. This title is a useful tool for acquiring basic knowledge of this field and following recent progress. It is a valuable contribution to the area of nanopore biosensing and is of interest to graduate students, postdocs, or senior researchers working in the fields of physical chemistry, biochemistry, bio- and electrical engineering, and biophysics.

This book provides an up-to-date overview of the biological functions of melanins, their biotechnological production, and their industrial applications. The melanins form a group of polymeric pigments found in most organisms. These natural products are generated by the oxidation of phenolic and indolic molecules yielding melanins, which include eumelanin, pheomelanin, pyomelanin, and the allomelanins. These pigments have diverse biological functions, including photoprotection, thermoregulation, antioxidation, virulence, and metal ion sequestration. Melanins have physicochemical properties that have proven useful in the optical, electronic, material, agriculture, cosmetic, pharmaceutical, and medical industries. The chapters in this book provide an in-depth analysis of the current issues and challenges in this field. Topics covered include the properties, biogenesis, and evolution of melanosomes. The book also addresses the synthesis and industrial production of melanins by microorganisms. The metabolic pathways for the synthesis of several types of polymeric pigments in microorganisms and their role in pathogenesis are reviewed. The large-scale production and purification of melanins to provide this product for industrial applications is a formidable challenge. Two chapters present and discuss the state-of-the-art used for the generation of microbial melanin-production strains and the processes for the biotechnological synthesis and purification of these pigments. Melanins are polymers that can interact with metals, a chapter reviews this capacity in the context of technological applications, including electrochemical energy storage and metal recovery. These polymers are materials having favorable characteristics for photonic applications, including UV blocking and broadband absorption. The current advances in the application of these pigments in optically active materials are presented and discussed.

This book focuses on the different compounds (polyphenols, sterols, alkaloids terpenes) that arise from the secondary metabolism of plants and fungi and their importance for research and industry. These compounds have been the backbone and inspiration of various industries like the food, pharmaceutical and others to produce synthetic counterparts. Furthermore, many of these compounds are still widely used to carry out specific functions in all these industries. This book offers a compilation of different texts from world leading scientists in the areas of chemistry, biochemistry, plant science, biotechnology which compile information on each group of secondary metabolism compounds, and their most important applications in the food, pharmaceutical, cosmetic and textile industry. By showcasing the best uses of these compounds, the chemistry behind their production in plants and fungi, this book is a valuable resource and a'go to'artifact for various audiences. The new approach this book offers, by linking research and the application of these compounds, makes it interesting as an inspiration for new research or as a hallmark of what has been done in the secondary metabolism of plants and fungi in recent years. Although this book may be technical, it is also enjoyable as an integral reading experience due to a structured and integrated flow, from the origins of secondary metabolism in organisms, to the discovery of their effects, their high intensity research in recent years and translation into various industries. Beyond learning more on their chemistry, synthesis, metabolic pathway, readers will understand their importance to different research and industry.

This book offers to provide a brief explanation of the key terms and concepts of Biochemistry. Biochemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism.

This book provides a comprehensive reference for various plant bioactive compounds for research and pharmacological significance across the entire spectrum of phytochemical genomics. The book opens with general information on diversity, analysis and genomic basis of phytochemicals, computational approaches, databases for responsible genes, and biosynthetic pathways, and it delves very much into the details behind phytochemical diversity and diverse roles of plant metabolites. The later parts of the book also explore the direct drug discovery and omics approaches including metabolomics, transcriptomics, as well as gene editing technology experiments to further inspire readers into its unlimited potentials. Each chapter includes detailed analysis and relevant experiments for better and deeper understanding of the concepts. The book will be an invaluable aid for medicinal plant researchers and a rich source of information and advice for advanced undergraduates and graduates in the fields of medicine, nutraceuticals, cosmetics, flavor, and fragrance studies.

This book provides comprehensive information on starch modification using physical approaches – a field that has attracted increasing interest in recent years due to the fact that it is no longer desirable to label starch a modified. The required functionalities can be conveniently achieved by physical methods that are less expensive and more environmentally friendly. In the second edition, chapters are updated according to the recent research progress. Three new chapters are added including pulsed electric fields, dry heating and physical treatments that produce chemical changes. Chapter one is rewrote into three individual chapters including Molecular Structure of Starch, Granular Structure of Starch and Physicochemical Properties of Starch, aiming to help the readers better understand the structure of starch. This book summarizes recent developments in the areas of starch physical modifications and reviews the structure, function and potential industrial applications of modified starch. It provides valuable information for researchers and product developers to work on starch.

Ideal for readers encountering biochemistry for the first time, Garrett and Grisham's BIOCHEMISTRY, Seventh Edition, makes even complex course concepts more accessible while revealing the beauty and usefulness of biochemistry in the everyday world. This engaging text illuminates the fundamental principles governing the structure, function and interactions of biological molecules, providing a solid foundation in biochemistry whether you are an undergraduate majoring in life sciences, chemistry or premedical programs, or a medical or graduate health sciences student seeking a deeper understanding of human physiology. Updated to reflect tremendous recent developments in biochemistry, the Seventh Edition features new and revised material and presentations throughout the text, incorporating additional content while maintaining a balanced and streamlined presentation. In addition, the authors emphasize the interrelationships of ideas to help you appreciate the overarching questions of biochemistry. The text is also integrated with OWLv2, a powerful online learning system for chemistry with text-specific end-of-chapter material to help you improve your grades and master course concepts.

The 3rd edition of this textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern chemistry, biochemistry and biophysics.Topics included are: electronic and vibrational absorption fluorescence symmetry operations and normal-mode calculations electron transfer from excited moleculesenergy transferexciton interactions electronic and vibrational circular dichroismcoherence and dephasingultrafast pump-probe and photon-echo spectroscopy single-molecule and fluorescence-correlation spectroscopyRaman scatteringmultiphoton absorption quantum optics and non-linear opticsentropy changes during photoexcitationelectronic and vibrational Stark effects studies of fast processes in single moleculestwo-dimensional electronic and vibrational spectroscopyThis revised and updated edition provides expanded discussions of laser spectroscopy, crystal symmetry, birefringence, non-linear optics, solar cells and light-emitting diodes. The explanations are sufficiently thorough and detailed to be useful for researchers, graduate students and advanced undergraduates in chemistry, biochemistry and biophysics. They are based on time-dependent quantum mechanics, but are developed from first principles so that they can be understood by readers with little prior training in the field. Additional topics and highlights are presented in special boxes in the text. The book is richly illustrated with color figures throughout. Each chapter ends with a section of questions for self-examination.

This book aims to provide an association of the major redox-sensitive pathways and networks involved in cancer. The initial chapter of the book discusses basic information about oxidative stress, its generation, and regulation (redox regulation or redox homeostasis) via cell signaling in normal and cancer cells. The book also explores antioxidants and metabolic events in cancer cells compared to normal cells. It further covers the application of nanoparticles in redox regulation in cancer cells. The role of redox regulation in cancer therapy, its influence, and its involvement in programmed cell death (PCD), metastasis, immune system, p53, and cell cycle/DNA damage repair pathways have been discussed in separate chapters. It further reviews the importance of dietary phytochemicals in redox regulation in normal and cancer pathophysiology. Towards the end, the book focuses on the role of redox balance, especially in ROS-dependent cellular processes in cancer stem cells.

This textbook for undergraduate students aims at providing an in-depth understanding of the relationship between diet, nutrients, health, diseases, and drug treatment. The book presents a comprehensive but detailed view of the field of Nutritional Biochemistry; balancing the historical with contemporary findings, the descriptive with the experimental, structure with function as well as the mechanistic and the clinical aspects of any particular nutrient. Though the major emphasis of the book is on Nutritional Biochemistry, the book also attempts to provide an insight into other related and relevant areas. Amongst the topics that are covered are: nutraceuticals, food, and nutrient interactions; the newly emerging field of the human microbiome, its interdependence on diet and human health as well as the public health concerns which is a looming burden of non-communicable diseases. Each chapter begins with an insight into the history of discovery and structure of the nutrient, its absorption, and metabolism, physiological functions, ending with diseases associated with nutrient deficiency/toxicity along with a clinical perspective. Apart from this, the book emphasizes the biochemical basis of physiological responses and correlates the same with symptoms identifying the pathophysiology. This textbook caters to students of undergraduate courses like Biochemistry, Biomedical Sciences, Biological Sciences, Life Sciences, Home Science; Nutrition and Dietetics, Clinical Nutrition and Dietetics, and Nursing. ​

This book focuses on the current status of our understanding of RNA, a key biological molecule. The various RNAs covered are messenger RNA, ribosomal RNA, transfer RNA, noncoding RNAs, modified nucleosides, and RNA enzymes. The different chapters detail methods to investigate RNA structure and function, the chemistry of modified RNAs, and the latest advances in our understanding of the vast array of biological processes in which RNA is involved.RNA, in one form or another, touches almost everything in a cell. RNA has both structural and catalytic properties. RNA fulfills a broad range of functions. These molecules are no longer seen as passive elements transferring the genetic information from DNA into proteins but regulate the activity of genes during development, cellular differentiation, and changing environments. RNAs are involved in various aspects of cell physiology and disease development. Discoveries of RNA with unexpected diverse functions in healthy and diseased cells,such as the role of RNA as both the source and countermeasure to cancer or severe viral infection, stimulate new trends, passion, and solutions for molecular medicine.In this book, fundamental questions about the biochemical and genetic importance of RNA, how mRNAs are generated and used to produce proteins, how noncoding and catalytic RNAs mediate key cellular processes, how to determine RNA structure and how to apply RNA in treatment of diseases.This book is an essential resource for researches in academia and industry contributing to the development of new RNA therapeutics. The book is geared toward scientists from the graduate level on up and particularly appeals to active investigators in RNA biology, molecular biology, and biochemistry.

This book overviews purinergic receptors that are playing key roles in human and pathophysiological processes. The book elaborates on how selective P1 and P2 modulators have been developed as therapeutics for a variety of diseases. It also provides an overview of current perspectives in the design of purinergic receptor modulators and future challenges such as the availability of selective ligands for all receptor subtypes.Divided into 12 chapters, this comprehensive volume also offers a multidisciplinary perspective on the historical evolution, starting with a chapter devoted to the roots and early discoveries of adenosine and its receptors, followed by a twenty-year retrospective on the synthesis, properties, and functional potential of adenosine receptor ligands, probes, and functional conjugates. In the next chapters, experts in the field delve into topics such as the therapeutic potential of adenosine receptor ligands in wound healing and fibrosis, the therapeutic benefits of A2A receptor antagonists, the A2B adenosine receptor as a target for brain ischemia or demyelination, the development and latest advancements in clinical trials of A3 adenosine receptor ligands. Other chapters describe bifunctional tools to study adenosine receptors, allosteric modulators of adenosine receptors, and new computational approaches to inspect adenosine receptor-ligand recognition processes. Furthermore, the book discusses the role of P2X4 receptors in immunity and inflammation. The final chapters illustrate CD73 inhibitors as antitumor agents, and bacterial ectonucleotidases as underexplored antibacterial drug targets. This book is a valuable resource for scholars working in the field of medicinal chemistry, as well as researchers in the industry, providing readers with a comprehensive understanding of adenosine receptor biology and its therapeutic potential.

This book highlights 12 major plants in the Asteraceae family from the aspects of traditional uses as food and medicine, phytochemistry, and pharmacological activities. Asteraceae is one of the largest family of flowering plants comprising over 1,600 plant genera and 32,000 plant species. Plants belonging to this family have a long history of being used as medicinal plants for the treatment of various diseases. Many of them are also used in the preparation of foods, beverages, and also used in pharmaceutical and cosmetic industries. In addition, plants such as Artemisia annua have played an important role in the discovery of novel drugs. The book summarizes the traditional uses of the plants in the family Asteraceae and their scientific validation, which helps readers understand their relation and impact on human health. It also explains the Phytochemistry of the species and presents the pharmacological activities and mechanisms in detail. Understanding current scientific knowledge will help in the commercialization of products based on these plants and also helps to find the research gaps that should be fulfilled in the future for their optimal use. It also helps in increasing the awareness of the plant species related to conservation, cultivation, and sustainable utilization. This edited volume comprises chapters contributed by experts from around the world.

This book covers edge-point applications in science and engineering. The chapters discuss the functional properties of advanced engineering materials and biomolecules, improving the comprehension of their chemical physical properties and potential for new technological and medicinal applications. The book presents a small number of experimental techniques and computational simulation models from basic concepts of classical/quantum mechanics, physics, chemistry, biology, statistical methods that can predict important applications and properties of these materials/biomolecules. The content shows how improving design of new systems helps in addressing future world problems (health, energy, food, environment, transportation, housing, clothing, etc.), i.e., almost every aspects of our daily lives.

This book provides an interdisciplinary review of one of the great unsolved mysteries that has fascinated scientists for over 150 years: the origin of chirality in biomolecules. Current advances in fields as diverse as space exploration, prebiotic chemistry and high-energy physics may help to provide an answer. Important pieces of information will come from observations at the two frontiers of science: outer space and the subatomic world. Observation of distant planets, galaxies, and even actual sampling of celestial objects from beyond the solar system are projects currently underway. At the other end of the spectrum, there are experiments that study the elemental properties of matter, such as symmetry, and interactions with the fundamental forces. Completely revised and updated this new edition once again unifies all the theories of the origin of biomolecular homochirality together in a single source. This complete, interdisciplinary review of an intriguing subject condenses a large and disparate range of contributions from journals in almost every scientific field. The various theories have been organized, interrelated and explained in a unified way. It is fundamental, comprehensive and structured to be accessible for educational purposes.

This second edition of Medical Biochemistry is supported by more than 45 years of teaching experience, providing coverage of basic biochemical topics, including the structural, physical, and chemical properties of water, carbohydrates, lipids, proteins, and nucleic acids. In addition, the general aspects of thermodynamics, enzymes, bioenergetics, and metabolism are presented in straightforward and easy-to-comprehend language. This book ties these concepts into more complex aspects of biochemistry using a systems approach, dedicating chapters to the integral study of biological phenomena, including cell membrane structure and function, gene expression and regulation, protein synthesis and post-translational modifications, metabolism in specific organs and tissues, autophagy, cell receptors, signal transduction pathways, biochemical bases of endocrinology, immunity, vitamins and minerals, and hemostasis. The field of biochemistry is continuing to grow at a fast pace. This edition has been revised and expanded with all-new sections on the cell plasma membrane, the human microbiome, autophagy, noncoding, small and long RNAs, epigenetics, genetic diseases, virology and vaccines, cell signaling, and different modes of programmed cell death. The book has also been updated with full-color figures, new tables, chapter summaries, and further medical examples to improve learning and better illustrate the concepts described and their clinical significance. - Integrates basic biochemistry principles with molecular biology and molecular physiology - Illustrates basic biochemical concepts through medical and physiological examples - Utilizes a systems approach to understanding biological phenomena - Fully updated for recent studies and expanded to include clinically relevant examples and succinct chapter summaries

Dieses Buch liefert einen sehr persönlichen Einblick in das Leben und Wirken von Felix Hoppe-Seyler. Er war durch seine Entdeckungen und seinen Einfluss auf die Entstehung eines neuen Forschungsgebietes, der Physiologischen Chemie oder Biochemie, eine der einflussreichsten Forscherpersönlichkeiten seiner Zeit. Hoppe-Seylers eigene Entdeckungen sind zahlreich. Sie schließen Erkenntnisse aus der Physiologie, der Pathologie und der Chemie ein. Im Mittelpunkt seiner Leistungen stehe aber die nie erlahmenden Bemühungen der Physiologischen Chemie, also der Wissenschaft von den chemischen Grundlagen der Biologie ihren Platz an den deutschen Universitäten zu erkämpfen. Seine Waffen waren die von ihm gegründete Zeitschrift für Physiologische Chemie und das Handbuch der Physiologischen- und Pathologischen Analyse. Georg Hoppe Seyler, ein Nachfahre, hat sich bemüht ein möglichst objektives, von überkommenen durch die Jahre veränderten Vorstellungen freies Bildauf der Grundlage von Briefen und Dokumenten zu zeichnen. Briefe des Nachlasses, Zitate von mit Felix Hoppe (-Seyler) arbeitenden Zeitgenossen die ihn als der Persönlichkeit und Wissenschaftler kennen lernten und seine Publikationen bilden den Hintergrund der kurzen Zusammenstellung.

Antimicrobial Peptides, Volume 663 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Unifying the classification of antimicrobial peptides in the Antimicrobial Peptide Database, Optimizing peptide library creation for PepSAVI-MS (RP libraries, etc.), Discovery of novel Antimicrobial peptides using BioProspecting, Screening for cysteine-stabilized scaffolds for developing protelytic-resistant AMPs, Exploring synergy and its role in antimicrobial peptide biology, Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens, and much more. Other chapters cover Liquid chromatography-mass spectrometry-based analysis of naturally occurring neuropeptide diastereomers, Multiplexed Quantitative Neuropeptidomics via DiLeu Isobaric Tagging, In vitro evaluation of antibiotic resistance via proteomics, Molecular networking-based strategies in mass spectrometry, Development of Macrocyclic antimicrobial peptides and peptoids, and a host of other timely topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Antimicrobial Peptides